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Citations for Promega :
1951 - 2000 of 3433 citations for Borrelia burgdorferi sensu stricto B31 CRASP 1 Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2020Quote: ... with specific primers (Suppl. Table 1) from pNL1.1 (Promega, Fitchburg, WI) and inserted between the SalI and BglII sites using NEBuilder HiFi DNA Assembly Master Mix ...
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bioRxiv - Molecular Biology 2022Quote: ... and then incubated with 1 μM TMR HaloTag ligand (Promega, G8252) for indicated time ...
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bioRxiv - Developmental Biology 2019Quote: ... mouse anti-β-galactosidase (1:1000, Z3783, Promega, Madison, WI, USA), rabbit anti-β-galactosidase (1:1000 ...
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bioRxiv - Molecular Biology 2019Quote: ... for 1 hour at 37 °C with RNasin Plus (N2611, Promega). The RNA was extracted with Acid Phenol:Chloroform:Isoamyl alcohol (125:24:1 ...
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bioRxiv - Molecular Biology 2019Quote: A 3:1 ratio of FuGENE® HD Transfection Reagent (Promega) (μL ...
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bioRxiv - Molecular Biology 2021Quote: ... with a combination of 1) pGL4 Firefly luciferase reporter (Promega, E6651), 2 ...
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bioRxiv - Genomics 2019Quote: ... Polypeptides were trypsin digested at a ratio of 1:50 (Promega), overnight at 37 °C ...
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bioRxiv - Biochemistry 2020Quote: ... and 1 ng of Renilla luciferase reporter with FuGene HD (Promega) in OptiMEM (Invitrogen ...
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bioRxiv - Cell Biology 2020Quote: ... The harvested organs were immersed in 1/400 diluted furimazine (Promega) in sterilized PBS to detect Nluc/BRET-FL signal ...
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bioRxiv - Immunology 2021Quote: ... then each well was diluted 1:3 with TE buffer (Promega). A 2.7 μL aliquot from each sample was mixed with 2.5 μL of SsoFast EvaGreen Supermix with Low Rox (Bio-Rad ...
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bioRxiv - Immunology 2020Quote: ... Following stimulation cells were lysed in 1 × Passive lysis buffer (Promega) and Firefly luciferase and Renilla luminescence were measured using the MARS data analysis software on the FLUOstar Omega Luminometer (BMG Labtech) ...
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bioRxiv - Genomics 2019Quote: ... Reverse transcription reactions containing 1 uL GoScript reverse transcriptase (Promega, #A501C) in a final volume of 20 uL were placed in a thermal cycler with program sequence ...
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bioRxiv - Cell Biology 2019Quote: ... and mouse anti-β-galactosidase (Z3781; Promega, Madison, WI; 1:200). Alexa Fluor 488-conjugated (A21206 ...
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bioRxiv - Biophysics 2019Quote: ... 10 μl/well of NanoLuc substrate (Promega, after 1:20 dilution) was added followed by addition of 10 μl/well indicated concentration of 4×compounds solutions ...
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bioRxiv - Genetics 2020Quote: ... Alkaline phosphatase-conjugated secondary antibodies were used at 1:10000 (Promega). Western blot band intensity was analyzed with ImageJ Gel quantification tool.
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The heterogeneity of the DNA damage response landscape determines patient outcomes in ovarian cancerbioRxiv - Cell Biology 2021Quote: ... Pellets were resuspended in 1% low melting point agarose (V2111; Promega) in dPBS ...
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bioRxiv - Microbiology 2020Quote: ... Finally samples were digested by trypsin (1/100, w/w, Promega) for 16 h at 37°C ...
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bioRxiv - Biophysics 2021Quote: ... and 1:5000 goat anti-rabbit IgG HRP-conjugate (Promega, Denmark). Samples used for incorporation controls were treated as above ...
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bioRxiv - Cell Biology 2022Quote: ... 100 mM KCl) supplemented with 1 mM Ultra-Pure ATP (Promega). 3 μl of dsRNA dilutions or water were added to the mix and reactions were immediately incubated at 30 °C for 20 min ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... cells were resuspended in HBSS containing Furimazine (1:50 dilution; Promega) and dispensed into 96-well half-area white plates ...
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bioRxiv - Biochemistry 2021Quote: ... The samples were digested with 1 μg/μl trypsin gold (Promega), chymotrypsin (Promega) ...
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bioRxiv - Developmental Biology 2021Quote: ... purified genomic DNA (with 1% unmethylated lambda DNA spike-in, Promega) was sheared to a fragment size of 100 - 700 bp (primary size 250 bp) ...
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bioRxiv - Genomics 2021Quote: ... and 1% unmethylated lambda DNA (cat#: D1521, Promega, Madison, WI, USA) was spiked in to measure bisulfite conversion efficiency ...
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bioRxiv - Cell Biology 2021Quote: ... Primary antibodies used were anti-p75NTR (Promega, Cat: G323A, 1:300) and anti-GAPDH (Sigma ...
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bioRxiv - Cell Biology 2022Quote: ... or anti-goat IgG HRP (Promega, Madison, WI, V805A, 1:10,000). Signal was detected with Radiance ECL (Azure ...
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bioRxiv - Cancer Biology 2022Quote: ... Nano-Glo Luciferase Assay Substrate furimazine (2 µl ml-1, Promega) was added and the nLuc released upon proteolytic cleavage of the receptor was measured on a plate reader Mithras LB 940 (Berthold Technologies ...
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bioRxiv - Cell Biology 2022Quote: ... incubated with HRP-conjugated donkey anti-mouse IgG (1:10,000; Promega), and revealed with Super Signal chemiluminescent substrate reagents (Pierce ...
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bioRxiv - Cell Biology 2022Quote: ... and 1 μl reverse transcription enzyme M-MLV-RT (Promega, USA). PCR was performed using each specific primer set in a total volume of 20 μl containing 10 pmol of each primer ...
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bioRxiv - Cancer Biology 2022Quote: ... and 1 U HotStarTaq DNA Polymerase and 250mM dNTPs (Promega, USA). PCR was performed with thermal conditions as follows ...
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bioRxiv - Molecular Biology 2023Quote: ... 0.5% bovine serum albumin] supplemented with RNAsin (1:1000, Promega, #N2511) for FACS purification ...
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bioRxiv - Neuroscience 2022Quote: ... and recombinant RNasin ribonuclease Inhibitor (1 uL/ml, Promega, Cat#N2511). The components of the high salt wash buffer are as follows ...
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bioRxiv - Genetics 2023Quote: ... and 1 unit of GoTaq G2 Flexi DNA Polymerase (Promega, USA). DNA concentrations was rarely quantified as amplification was often successful across a range of values (e.g ...
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bioRxiv - Microbiology 2023Quote: ... The lysis buffer contained 1:4000 NanoGlo (furimazine) substrate (Promega, USA), to generate a bioluminescence signal through substrate hydrolysis 59 ...
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bioRxiv - Molecular Biology 2023Quote: ... 1 µL of 100 ng/µL random hexamer primers (Promega: C1181) was added to each sample ...
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bioRxiv - Molecular Biology 2023Quote: ... this reaction was spiked at 1% into a GoTaq HotStart (Promega) PCR reaction with primers P3HDV and P5XHPγn ...
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bioRxiv - Evolutionary Biology 2023Quote: ... Each 25 μL PCR consisted of 1 x GoTaq Buffer (Promega), 3 μM MgCl2 ...
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bioRxiv - Cell Biology 2023Quote: ... The following antibodies were used: HaloTag mAB (Promega, G9211, 1:500), GAPDH (Cell Signaling 2118 ...
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bioRxiv - Evolutionary Biology 2023Quote: ... a DNase I (RQ1 RNASE-FREE DNASE 1* from Promega®) treatment was done to all samples ...
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bioRxiv - Genomics 2023Quote: ... 1 μl of RNasin Plus RNase Inhibitor (catalog num. N2611, Promega), 4 μl of nuclease-free water (UltraPure DNase/RNase-Free Distilled Water ...
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bioRxiv - Genomics 2023Quote: ... 1 mM EDTA) and quantified by QuantiFluor® dsDNA System (Promega). The DNA (1-2 µg DNA / 100 µl volume ...
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bioRxiv - Biophysics 2023Quote: ... and covalent anchoring of 20 µg·mL−1 HaloTag amine ligand (Promega) in PBS overnight ...
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bioRxiv - Microbiology 2023Quote: ... using the GoTaq® Probe 1-Step RT-qPCR System (Promega). Methods for the generation of the 18S and sgE RNA standards have been outlined previously.31 Both PCR products were serially diluted to produce standard curves in the range of 5 x 108 - 5 copies/reaction via a 10-fold serial dilution ...
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bioRxiv - Microbiology 2023Quote: ... using the GoTaq® Probe 1-Step RT-qPCR System (Promega). Quantification of 18S RNA utilised previously described primers and probe sequences,30 and were used at 300 nM and 200 nM ...
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bioRxiv - Biochemistry 2022Quote: ... 20 μl of 5 ng·μl−1 trypsin (sequencing grade, Promega®) in 50 mM ABC ...
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bioRxiv - Cancer Biology 2023Quote: ... and cloned into a pGEM-T Easy Vector System 1 (Promega). Plasmid DNA from ~ 20 positive clones for each cell line was isolated using QIAprep Spin Miniprep Kit (Qiagen ...
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bioRxiv - Genetics 2023Quote: ... and 1 unit of GoTaq G2 Flexi DNA Polymerase (Promega, USA). Thermocycling conditions for S ...
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The tetrapeptide sequence of IL-1β regulates its recruitment and activation by inflammatory caspasesbioRxiv - Immunology 2023Quote: ... and pMD2.G (1 μg) using Fugene HD transfection reagent (Promega) into HEK 293T cells ...
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bioRxiv - Cancer Biology 2022Quote: ... The harvested organs were immersed in diluted Fz (1/400; Promega) in sterile PBS then imaged using IVIS (Perkin Elmer ...
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bioRxiv - Immunology 2023Quote: ... 120 ng plasmid of interest and 1 µl FuGENE HD (Promega) diluted to 10 µl total volume with OptiMEM.
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bioRxiv - Plant Biology 2023Quote: ... 40 µL of trypsin 1:100 (Sequencing grade modified trypsin, Promega) in 50 mM ammonium bicarbonate were added to the sample and incubated in a wet chamber at 37 °C overnight ...