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Citations for Promega :
1951 - 2000 of 2934 citations for 1 Methoxy 4 1 methylbutyl benzene since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2024Quote: ... From there on all buffers were supplemented with 1:100 RNasin Plus RNase inhibitor (Promega, Cat. No. N2615).
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bioRxiv - Cancer Biology 2024Quote: ... G17-1 cell proliferation was measured by MTS assay (Cell Titer 96 Aqueous One, Promega, Madison, WI, USA).
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bioRxiv - Bioengineering 2024Quote: The primary antibodies used in this study were the following: rb-α-Nanoluc (non-commercial, Promega, 1:1000); rb-α-CD63 (ab68418 ...
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bioRxiv - Molecular Biology 2024Quote: ... flies were homogenized in 0.2N NaOH + 1% DTAB and processed according to the manufacturer’s instructions: NAD+ (Promega # G9071), cADPR (AAT Bioquest # 20305) ...
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bioRxiv - Cancer Biology 2024Quote: ... Beads were then resuspended in 100 µL of 50 mM ABC containing 1 mg trypsin (Promega, Cat. # V5113) and 0.1 mAu Lys-C (Wako Chemicals ...
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bioRxiv - Microbiology 2024Quote: ... Proteins were then digested into peptides by the addition of 1:1000 (g/g) Lys-C Protease (Promega) for 4 h at 37°C ...
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bioRxiv - Molecular Biology 2024Quote: ... samples were incubated with 80 μL of 2 M urea in 50 mM Tris-HCl (pH 7.5) containing 1 mM DTT and 0.5 μg trypsin (Promega) at 25°C with shaking for pre-digestion ...
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bioRxiv - Neuroscience 2024Quote: ... cDNA was generated from 1 μg total RNA per sample using the M-MLV Reverse Transcriptase kit (Promega). To detect RNA transcript of targeted genes ...
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bioRxiv - Microbiology 2024Quote: Swabs collected into 1% BSA in PBS were spun down using DNA IQ Spin Baskets (Promega, Madison, WI) and each sample was split into two equal 75 µl portions ...
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bioRxiv - Neuroscience 2024Quote: ... excised from the gel and protein enzymatic cleavage was carried out with trypsin (Promega; 1:20, w/w) at 37 °C for 16 h as previously described (Shevchenko et al. ...
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bioRxiv - Cancer Biology 2024Quote: ... Beads were then resuspended in 100 µl of 50 mM ABC containing 1 µg trypsin (Promega, Cat. # V5113) and 0.1 mAu Lys-C (Wako Chemicals ...
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bioRxiv - Bioengineering 2024Quote: ... All RNA samples first underwent a DNAse step (1 µL DNAse/12.2 µLRNA, RQ1 RNAse-Free DNAse, Promega), then reverse transcription using a High-Capacity cDNA Reverse Transcription kit (Thermo Fisher Scientific ...
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bioRxiv - Cancer Biology 2024Quote: ... Samples were washed twice with 50 mM HEPES, pH 8.5 (18,000g, 2 x 10min) before they were incubated with 1 μg trypsin (Promega) in 50 mM HEPES ...
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bioRxiv - Cancer Biology 2024Quote: ... cells were seeded in 96-well plates at 3,000-5,000/well in serial 1:2 dilutions for 72 hours and assessed by CellTiter-Glo (Promega). For synergy assays ...
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bioRxiv - Developmental Biology 2024Quote: ... The amount of enriched methylated DNA from 1 μg total DNA was measured using a Quantus Fluorometer (Promega). Libraries for MBD-Seq analysis were prepared using a NEBNext Ultra II DNA Library Prep Kit for Illumina according to the manufacturer’s instructions and validated for an average size of about 550-600 bp using a High Sensitivity DNAAssay kit ...
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bioRxiv - Cancer Biology 2021Quote: ... and then resuspended in 4 ml DMEM/F12 + 80 μl DNase (1U/μl) (Promega M6101). The DNase solution was gently shaken by hand for 2–5 min ...
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bioRxiv - Molecular Biology 2021Quote: ... Gel pieces were rehydrated in a mixture of 4 ng/µL trypsin (Promega, Madison, WI) and 0.01% ProteaseMAX (Promega ...
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bioRxiv - Cell Biology 2020Quote: ... followed by a 4 times dilution in Tris buffer and an overnight trypsin digestion (Promega) at a ratio 1/100 ...
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bioRxiv - Molecular Biology 2020Quote: ... Resuspended grindate was incubated (4°C, 20 min) with 660 U of DNase I (Promega). Insoluble cell debris was pelleted by spinning (16,000 g ...
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bioRxiv - Cell Biology 2020Quote: ... Cultures were then incubated overnight at 4°C with either anti-β3-tubulin (Promega #G712A) or anti-Oct4 (Santa Cruz Biotechnologies #SC-5279 ...
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bioRxiv - Molecular Biology 2021Quote: ... Protein samples were digested using 4 μg of trypsin (sequencing grade, low autolysis trypsin; Promega), at 37 °C for 16 h ...
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bioRxiv - Microbiology 2023Quote: ... 200 ml of media was treated with 4 U of RQ1 RNase-free DNase (Promega) for 1 hour at 37°C ...
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bioRxiv - Plant Biology 2024Quote: ... 4 hours incubation at 37 °C) and 5 μg of trypsin (Trypsin Gold from Promega, in 50 mM TEAB pH 8.5 ...
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bioRxiv - Molecular Biology 2023Quote: ... RNA (4 μg) was reverse-transcribed using oligo-dT and the Reverse Transcription System (Promega). cDNA samples were diluted 1:10 and 5 μl of each sample were used for PCR reactions (final volume of 20 μl ...
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bioRxiv - Biochemistry 2023Quote: ... The pellet was resuspended in 4 M urea containing 0.1 % Protease Max (Promega Corp. V2071) and diluted in 40 mM ammonium bicarbonate buffer ...
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bioRxiv - Genetics 2022Quote: ... cells were transfected with L1 reporter constructs using 4 μl FuGENE HD transfection reagent (Promega), 96 μl Opti-MEM (Life Technologies ...
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bioRxiv - Cancer Biology 2023Quote: ... Fisher) containing 4% FBS with or without HaloTag NanoBRET 618 Ligand (Cat. No. PRN1662, Promega) and incubated overnight at 37 °C ...
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bioRxiv - Biochemistry 2023Quote: ... The pellet was resuspended in 4 M urea containing 0.1 % Protease Max (Promega Corp. V2071) and diluted in 40 mM ammonium bicarbonate buffer ...
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HIVtat Alters Epithelial Differentiation State and Increases HPV16 Infectivity in Oral KeratinocytesbioRxiv - Microbiology 2023Quote: ... 200 ml of media was treated with 4 U of RQ1 RNase-free DNase (Promega) for 1 hour at 37°C ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: Cells were fixed with 4% paraformaldehyde and permeabilized in 0.5% Triton X-100 (Promega, H5142) prior to staining ...
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bioRxiv - Plant Biology 2020Quote: ... First-strand cDNA was synthesized from 1 μg total RNA using the M-MLV Reverse Transcriptase (Promega, Cat. #M1705) (primers are listed in Supplementary Table 8) ...
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bioRxiv - Cancer Biology 2021Quote: ... The digested vector (200 ng) was ligated with 1 µL of the oligonucleotides (0.45nmol/μl) using T4 DNA ligase (Promega) for 3 hrs ...
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bioRxiv - Microbiology 2020Quote: ... ratio 50:1 (protein:rLysC) for 3h/37°C and then digested with Sequencing Grade Modified Trypsin (#V5111, Promega, France) ratio 50:1 (protein:trypsin ...
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bioRxiv - Microbiology 2020Quote: ... Cytosine tails (C-tails) were added to 1 µg sheared DNA using terminal deoxynucleotidyl transferase (TdT, Promega, Madison, WI). Transposon containing fragments were amplified in a PCR containing DNA from the TdT reaction as template and primers specific to the ColE1 site on the 5’ end of the transposon ...
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bioRxiv - Developmental Biology 2021Quote: ... cells were washed twice (3000 x g for 5 min at 4°C) and resuspended in RNAse-free PBS with 1% BSA and 0.5 U/ul RNase inhibitor (Cat. N2615, Promega). Cells were then used for the 10x Genomics Chromium Single Cell System (10x Genomics ...
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bioRxiv - Molecular Biology 2022Quote: ... 0.05% (v/v) NP-40 and 0.5% (v/v) Triton X-100) containing 20 U ml−1 of RNasin (Promega), 1 mM DTT ...
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bioRxiv - Neuroscience 2021Quote: ... cells were washed twice (3000 x g for 5 min at 4°C) and resuspended in RNAse-free PBS with 1% BSA and 0.5 U/ul RNase inhibitor (Cat. N2615, Promega). Cells were then used for the 10x Genomics Chromium Single Cell System (10x Genomics ...
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bioRxiv - Molecular Biology 2021Quote: ... Fujifilm) and then incubated for 8 h at 37 °C with sequence-grade trypsin (1□g; Promega, Madison, Wisconsin). Peptides were desalted on Ultra Micro Spin Columns C18 (The Nest Group Inc. ...
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bioRxiv - Molecular Biology 2020Quote: Gently re-suspend cells in 100 μl of 3% Glyoxal fixation solution with 1:25 RNasin Plus (Promega N261B) and incubate for 15 minutes on ice.
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bioRxiv - Molecular Biology 2021Quote: ... Total RNA was quantified to 3 μg to react 1 μg/μL random hexamer (C1181; Promega, Madison, WI, USA) at 70°C for 5 min ...
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bioRxiv - Microbiology 2021Quote: ... 1 μL of forward and reverse primer (5 μM, see Supplementary Table 1) and 5.5 μL of Nuclease-free Water (Promega). The PCR I was performed as follows ...
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bioRxiv - Immunology 2021Quote: Total RNA was extracted from sorted cells with RiboEx (GeneAll) and 0.2–1 μg was subjected to cDNA synthesis using the GoScript Reverse Transcription system (Promega) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... The 1/10 of the sample was kept for injection and the 9/10 were digested with trypsin (Promega) (12.5 ng/µl ...
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bioRxiv - Plant Biology 2020Quote: ... 10 mg/L BSA and 1 mM PMSF) and assayed using the Dual-Luciferase® Reporter Assay System (Promega). Experiments were normalized to negative control set at 1.
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bioRxiv - Synthetic Biology 2021Quote: ... 1 µl of this crude lysate was used for PCR in a 10 µl GoTaq Green reaction (Promega M7123) with 0.25 µM of primers ...
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bioRxiv - Developmental Biology 2020Quote: ... Postnatal testes chemically synchronized for undifferentiated spermatogonia were homogenized at 2% w/v in polysome buffer (50 mM Tris, pH 7.4, 100 mM KCl, 12 mM MgCl2, 1% Nonidet P-40, 1mM DTT, 200 U/mL Promega RNasin ...
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bioRxiv - Biochemistry 2021Quote: ... The next day the sample was further diluted to 1 M urea and 750 ng Glu-C (Promega, V1651) were added and incubated for another 8 hours at 37°C.
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bioRxiv - Neuroscience 2021Quote: ... the supernatant was removed and the nuclei pellet was resuspended into 500ul nuclei storage buffer (NSB: 1x PBS; Invitrogen, 0.5% RNase free BSA;Serva, 1:200 RNaseIN plus inhibitor; Promega, 1x EDTA-free protease inhibitor ...
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bioRxiv - Cell Biology 2021Quote: ... membranes were subjected to three wash periods for 5 minutes in 1x TBS with 0.5% Tween and then probed with horseradish peroxidase–conjugated goat anti-mouse or goat anti-rabbit secondary antibodies at 1:2,500 (W4021, W4011, Promega) in 1x TBS with 5% nonfat dry milk and 0.5% Tween at room temperature for 1 hour ...
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bioRxiv - Cancer Biology 2021Quote: ... 1 mg/mL 50 μL aliquots of cleared lysates were incubated with 2 μL of indicated trypsin concentration (Promega) at 4°C for 15 min ...