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Citations for Promega :
1951 - 2000 of 2982 citations for 1 6 Bismaleimidoethane since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2022Quote: ... cDNA synthesis was prepared with 1 µg of RNA using an AMV reverse transcription system (Promega) and random primers according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2020Quote: ... Proteins were digested by incubation with sequencing-grade modified trypsin (1/50 w/w; Promega,V5113) for 12 h at 37°C ...
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bioRxiv - Neuroscience 2021Quote: ... HEK-293 cells were transfected with pmirGLO Dual-Luciferase miRNA Target Expression Vector (1 μg, Promega) containing a control reporter gene Renilla luciferase (hRluc ...
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bioRxiv - Microbiology 2020Quote: ... 1 μg of genomic DNA from the corresponding isolates was digested by EcoRI (Promega, Madison, WI), the amplified DNA fragments were subjected to ethanol precipitation (65) ...
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bioRxiv - Cell Biology 2021Quote: ... Samples were then subjected to immunoblotting and probed for anti-p75NTR (Promega, Cat: G323A, 1:300) and anti-GAPDH (Sigma ...
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bioRxiv - Cell Biology 2021Quote: ... for 4 h at 800 rpm and 42°C or thermolysine (1:50) (Promega, Walldorf, Germany) for 2 h at 800 rpm and 60°C ...
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bioRxiv - Biochemistry 2021Quote: ... Reverse transcription was performed with 1 unit of Avian Myoblastosis Virus (AMV) reverse transcriptase (Promega®) at 42°C for 2 min ...
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bioRxiv - Developmental Biology 2022Quote: ... first-strand cDNA was synthesized using 1 µg total RNA with MMLV Reverse Transcription Kit (Promega) and poly-T primer ...
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bioRxiv - Cancer Biology 2022Quote: ... 10 µL of PBS containing 4 μg/mL Hoechst and 1/10000 CellTox Green Dye (Promega) were were dispensed per well 1 h prior to imaging at Cytation5 image cytometer or Opera Phenix (Perkin Elmer ...
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bioRxiv - Cell Biology 2020Quote: ... DLD-1 cells were transfected with guide plasmids and donor plasmid using ViaFect™ (#E4981, Promega) on 3.5cm dishes ...
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bioRxiv - Cancer Biology 2019Quote: ... and trypsin digestion was performed with a 1:50 mass ratio of sequencing-grade trypsin (Promega) to total protein for 16h at 37°C ...
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bioRxiv - Molecular Biology 2019Quote: ... 1mM DTT and RNasin Plus (1:50) and 25 units of ProTEV Plus Protease (Promega V6101) and incubated 2 hrs at 30°C ...
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bioRxiv - Cancer Biology 2019Quote: ... were secondary incubated with anti-mouse IgG (H+L) horseradish peroxidase coupled (1:3,000, W402b, Promega) and polyclonal anti-rabbit IgG (1:5000 ...
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bioRxiv - Developmental Biology 2020Quote: ... Primary antibodies used were: rabbit and mouse anti-βGal (1/1000; Promega and MP Biomedicals, respectiveley), rat anti-Ser (1/1000 ...
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bioRxiv - Cell Biology 2021Quote: ... 400 ng of RNA was treated with 1 unit of RQ1 RNase-Free DNase (Promega, M610A) at 37°C for 30 minutes and was inactivated by RQ1 DNase Stop Solution at 65°C for 10 minutes ...
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bioRxiv - Molecular Biology 2021Quote: ... The adapter ligated aRNA was then mixed with 1 µl of dNTPs (10 mM each) (Promega) and 2 µl of RTP oligonucleotide (20 µM ...
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bioRxiv - Neuroscience 2019Quote: ... Proteins were digested overnight at 37°C with 1 μg of mass spectrometry grade Trypsin (Promega). The resulting peptide samples were cleaned up for mass spectrometry in a Sep-Pak C18 column (Waters ...
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bioRxiv - Cancer Biology 2020Quote: ... and then with 1:5000 of anti-rabbit (#W401B) or anti-mouse (#W402B) secondary antibodies (Promega).
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bioRxiv - Biochemistry 2021Quote: ... along with Renilla luciferase (50 ng of pGL4.70[hRluc] or 1 ng of pRL-null; Promega), and pCI expression vector encoding either FL-PC1 ...
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bioRxiv - Systems Biology 2021Quote: ... Both 1° and 2° assays were performed with 2x PCR master mix (Promega Corporation, Wisconsin, USA). The final PCR product from the 2° nested PCR was checked by electrophoresis in a 1.5% agarose gel ...
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bioRxiv - Biochemistry 2021Quote: ... followed by adding sequencing grade Trypsin at the ratio of 1:50 (Promega, cat. no. V5111) for digestion at 37 °C overnight ...
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bioRxiv - Microbiology 2021Quote: ... Viral RNA was quantified by real-time RT-qPCR (GoTaq 1 step RT-qPCR kit, Promega). Quantification was provided by serial dilutions of an appropriate T7-generated synthetic RNA standard ...
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bioRxiv - Synthetic Biology 2021Quote: ... The remaining cells were harvested and lysed with 30 μl of 1× passive lysis buffer (Promega).
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bioRxiv - Neuroscience 2021Quote: ... 25 uL of lysate was mixed with NanoGlo substrate diluted 1:50 in NanoGlo buffer (Promega) and 25 uL of lysate was mixed with ONE-Glo luciferase assay buffer (Promega ...
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bioRxiv - Bioengineering 2021Quote: ... RNA (1 μg) was subjected to RT-PCR in accordance with the protocol provided by Promega. The transcripts were quantitated and normalized to the internal GAPDH control ...
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bioRxiv - Bioengineering 2021Quote: ... The 1 μg linearized plasmid was serving as a template for in vitro transcription (SP6, Promega), and the products was then transfected into cells with Lipofectamine 2000 (Invitrogen) ...
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bioRxiv - Biochemistry 2021Quote: ... precipitated by trichloroacetic acid precipitation and digested with 1:50 (w/w) chymotrypsin (Promega, Cat #V106A) in 100 mM Tris-HCl and 10 mM CaCl2 for 18 h at 37 °C with shaking ...
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bioRxiv - Developmental Biology 2021Quote: ... and MleKlfX were amplified from cDNA (Table 1) and cloned into pGEM-T Easy vector (Promega). The cloned fragments were used as templates for in vitro transcription (MEGAscript ...
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bioRxiv - Molecular Biology 2021Quote: HEK-293 cells treated with 1 μM thapsigargin were lysed in 1X Passive Lysis Buffer (Promega) supplemented with Halt™ Protease and Phosphatase Inhibitor Cocktail (ThermoScientific ...
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bioRxiv - Immunology 2021Quote: ... Purified mAb (2 mg/mL in PBS) was treated with 1 unit of IdeS protease (Promega) per 1 µg of mAb ...
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bioRxiv - Genetics 2019Quote: ... As secondary antibodies horseradish peroxidase (HRP)-conjugated α-mouse or α-rabbit IgG (Promega, 1:4000) were used ...
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bioRxiv - Genetics 2020Quote: ... 1 microgram of total genomic DNA (gDNA) was added with 0.1% of unmethylated lambda DNA (Promega), followed by one-hour digestion with three enzymes (MspI ...
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bioRxiv - Cell Biology 2022Quote: ... After two days, images were captured by CCD (TANON 5200, China) after 1 mM luciferin (Promega) was sprayed onto the leaves ...
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bioRxiv - Immunology 2022Quote: ... Supernatants were harvested and subjected to Caspase-Glo 1 inflammasome assay following manufacturer’s protocol (G9951, Promega). Luminescence from caspase-1 activity was detected with a FLUOstar Omega plate reader (BMG ...
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bioRxiv - Biochemistry 2022Quote: ... The protein from lysed cells were digested in 1 µL 10ng/µL of trypsin/LysC (Promega) in 100mM TEAB (ProtiFi) ...
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bioRxiv - Cancer Biology 2022Quote: ... On-bead tryptic digestion was performed with 1 μg MS-grade TPCK trypsin (Promega, Madison, WI) dissolved in 200 μl of 50 mM ammonium bicarbonate pH 8.3 overnight at 37°C ...
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bioRxiv - Cancer Biology 2022Quote: ... 20 µL of PBS containing 10 μg/mL Hoechst and 1/4000 CellTox Green Dye (Promega) were dispensed per well 8 h prior to imaging at Opera Phenix (Perkin Elmer ...
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bioRxiv - Genomics 2022Quote: ... Sections were then incubated with a horseradish peroxidase-labeled anti-rabbit antibody (1:300, Promega, W401B) for 1 hour at room temperature ...
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bioRxiv - Genomics 2022Quote: ... the NE1 and Wash buffers were supplemented with 1 U/μL of RNasin Ribonuclease Inhibitor (Promega).
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bioRxiv - Plant Biology 2022Quote: ... for 2 h as primary antibody and peroxidase-conjugated goat anti-rabbit antibody (1:10,000; Promega) for 1 h with 5 times 10 min washes in-between the incubations ...
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bioRxiv - Microbiology 2022Quote: ... 1 μL of ligation product was amplified using PCR with a GO Taq DNA polymerase (Promega) and Illumina primers (P7 5’-CAAGCAGAAGACGGCATACGAGATAGACCGGGGACTTATCATCCAACCTGT-3’ ...
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bioRxiv - Molecular Biology 2023Quote: RNA isolated from cells (1 μg) was digested using the RQ1 RNase-Free Dnase (M6101, Promega) and reverse-transcribed using the High-Capacity cDNA Reverse Transcription Kit (4368814 ...
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bioRxiv - Microbiology 2022Quote: ... The solution was diluted to below 1 M urea and digested using sequencing grade Trypsin (Promega). The proteolytic peptides were finally desalted using an Oasis HLB solid phase extraction well plate (Waters ...
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bioRxiv - Microbiology 2022Quote: ... Reactions were performed at 1/5th volume with the inclusion of Transcend Biotin-Lysyl-tRNA (Promega) to allow antibody independent detection of all translated products ...
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bioRxiv - Plant Biology 2022Quote: ... 500 ng of total RNAs were treated with 1 unit of RQ1 RNase-Free DNase (Promega) for 30 min at 37 °C ...
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bioRxiv - Microbiology 2023Quote: ... 15 µl samples were allowed to cool to room temperature and 1 µl RQ1 DNase (Promega) diluted to 0.1 U/µl in storage buffer (10 mM Hepes pH 7.5 ...
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bioRxiv - Molecular Biology 2023Quote: ... Luminescence was measured at 1 day post infection using the Nano-Glo Luciferase Assay System (Promega) on the Envision plate reader after cell lysis with 1X passive lysis buffer (E1941 ...
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bioRxiv - Biophysics 2023Quote: ... the proteins were digested into tryptic peptides by incubation with 1□µg sequencing grade trypsin (Promega) overnight at 37□°C ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... the cells were transfected with 1 or 1.5 μg plasmid DNA using FuGene HD (Promega, #E2311) according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2023Quote: ... the cells were co-transfected using Fugene HD transfecting reagent at a 1:3 ratio (Promega) along with pRL-TK Renilla (Renilla Luciferase ...