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Citations for Promega :
151 - 200 of 5483 citations for rno mir 16 Real time RT PCR Detection Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2022Quote: ... RT-PCR to validate splicing changes was performed using GoTaq (Promega) and quantified with Bioanalyzer RNA 6000 Nano assay (Agilent).
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bioRxiv - Neuroscience 2021Quote: ... RT-PCR analyses were performed using GoTaq DNA polymerase (#M7841, Promega) in a reaction volume of 25 μl comprising 1 μl of cDNA and primer pairs specific for the 5’-end (exon 1-3 ...
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bioRxiv - Genetics 2021Quote: ... RT-PCR was carried out using GoTaq Green Master Mix (Promega), following recommended protocol ...
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bioRxiv - Cell Biology 2023Quote: ... RT-PCR was performed using GoTaq Green Master Mix (Promega, M7123). 2 µl of diluted cDNA was added to 12.5 µl GoTaq Green Master Mix and 0.4 µM of forward and reverse primers and nuclease-free water in a 25 µl reaction ...
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bioRxiv - Developmental Biology 2019Quote: ... RT-qPCR was performed with GoTaq® qPCR Master Mix for Dye-Based Detection (Promega, A6001) using a CFX Connect Bio-Rad qPCR System ...
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bioRxiv - Pathology 2021Quote: RT-qPCR (SARS-CoV-2 and MS2 viral genome detection) were performed with the GoTaq 1-step qRt-PCR kit (Promega) using 3.8µL of extracted RNA and 6.2µL of RT-qPCR mix that contains 250nM of each primer and 75nM of probe ...
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bioRxiv - Molecular Biology 2022Quote: ... and RNA extracted (Maxwell 16 LEV simplyRNA purification kit, Promega).
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bioRxiv - Genomics 2023Quote: ... using the Maxwell® 16 LEV plant RNA kit (Promega). The RNA concentration was measured by Nanodrop (Thermofisher) ...
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bioRxiv - Cancer Biology 2024Quote: ... Then Maxwell 16 FFPE Plus LEV DNA Purification Kit (Promega) was used to extract FFPE DNA ...
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bioRxiv - Molecular Biology 2023Quote: ... Libraries were amplified by 16 cycles of PCR with GoTaq Flexi DNA Polymerase (Promega), purified using Agencourt AMPure XP (Beckman Coulter ...
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bioRxiv - Plant Biology 2024Quote: ... mRNA accumulation was assessed by real-time qPCR (GoTaq® qPCR Master Mix, Promega) and expression values were normalized to the expression of plant genes At4g26410 and At3g01150 as previously described as a stable reference genes (54) ...
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bioRxiv - Cancer Biology 2022Quote: ... Using the dual-luciferase detection kit (Promega #E1910), cells were lysed in the lysis buffer for 30 min ...
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bioRxiv - Biochemistry 2023Quote: ... 15 μl of Kinase detection reagent (Promega kit) was added to the wells ...
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bioRxiv - Neuroscience 2021Quote: ... and used for reverse transcription (Promega RT kit) to generate cDNA ...
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bioRxiv - Molecular Biology 2021Quote: ... Quantitative RT-PCRs were performed with the GoTaq qPCR Master Mix (Promega), 2% of cDNA per reaction ...
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bioRxiv - Plant Biology 2019Quote: ... RT-PCR was performed using GoTaq® DNA polymerase (Promega, https://www.promega.com) and corresponding primers for 28 cycles ...
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bioRxiv - Microbiology 2022Quote: Semi-quantitative RT-PCR was carried out using GoTaq DNA polymerase (Promega) with 16S rRNA primers (16S F and 16S R ...
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bioRxiv - Molecular Biology 2023Quote: RT-PCR was performed using the GoTaq G2 DNA Polymerase (M7845, Promega) with 3 μl of reverse transcriptase product and the following primers ...
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bioRxiv - Plant Biology 2023Quote: ... and RT-PCR was performed using Go-Taq polymerase (Promega, WI, USA).
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bioRxiv - Molecular Biology 2024Quote: ... RT-qPCR was performed using SYBR Green PCR Master Mix (A6002, Promega) with the gene-specific primers (Table S9) ...
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bioRxiv - Evolutionary Biology 2022Quote: ... column purification kits or Maxwell 16 LEV simplyRNA Purification Kits (Promega, Madison, WI, USA) and prepared cDNA libraries using TruSeq RNA Library Prep Kit (Illumina ...
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bioRxiv - Zoology 2023Quote: Total RNA was extracted using the Maxwell 16 LEV Plant RNA Kit with the Maxwell 16 Research Instrument (Promega, USA) following the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2021Quote: ... Samples were washed twice with UA buffer and twice with 50mM ammonium bicarbonate prior to digest of the immobilized proteins on the filter for 2 h at RT using 1 mg Lys-C (Wako Chemicals) and for 16 h at 37C using 2 mg trypsin (Promega). Tryptic peptides were collected by centrifugation (10 min at 14,000 g) ...
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bioRxiv - Microbiology 2023Quote: RT-qPCR was performed using the GoTaq 1-Step RT-qPCR kit (Promega Cat. No. A6020) using nifH and 16S rRNA (reference gene ...
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bioRxiv - Zoology 2024Quote: One-step RT-qPCR was conducted using GoTaq 1-Step RT-qPCR System kit (Promega, France) in total reaction volume of 20 µl containing 2 µl of RNA extracts and 300 nM of the same forward and reverse primers as above ...
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bioRxiv - Cancer Biology 2020Quote: The wild-type 3’UTR region of SALL4 mRNA or a mutant without the miR-205 binding site (Figure 4E) was amplified using PCR and cloned into the pGL3 vector (Promega, Madison, USA). HEK 293T cells were seeded into 24-well plates ...
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bioRxiv - Microbiology 2022Quote: ... was extracted with Maxwell® 16 LEV miRNA Tissue Kit (Promega) according to the manufacturer’s instructions and was subjected to an extra TURBO DNase (Invitrogen ...
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bioRxiv - Developmental Biology 2019Quote: GENEprint PowerPlex 16 kit (Promega performed by Cell Line Genetics. LLC) was used for STR analysis.
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bioRxiv - Molecular Biology 2021Quote: ... RNA was obtained using a Maxwell 16 LEV simplyRNA kit (Promega). One microgram of total RNA was used for PAT assays as described in Sallés and Strickland (1995) ...
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bioRxiv - Cancer Biology 2022Quote: ... GB02 and GB03 using the Maxwell® 16 Instrument with the Maxwell® 16 Tissue DNA Purification Kit (Promega, Madison, WI). DNA concentration was determined using the Qubit Fluorometer (Life Technologies ...
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bioRxiv - Microbiology 2021Quote: ... DNA was extracted from samples using Maxwell® 16 automated system (Maxwell® 16 Cell DNA kits, Promega, Madison, WI, USA). Extracted DNA samples were quantified using NanoDropTM 2000 UV-Vis Spectrophotometer (Thermo Fisher Scientific ...
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bioRxiv - Genetics 2019Quote: ... with a Maxwell 16 instrument using Maxwell 16 LEV SimplyRNA Blood kits according to the manufacturer’s protocol (Promega, Madison, WI, USA). Complementary DNA (cDNA ...
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bioRxiv - Cell Biology 2019Quote: ... RT-PCR reactions were conducted using the GoTaq qPCR Master Mix (A6001, Promega) and a MasterCycler Ep-Realplex thermal cycler (Eppendorf).
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bioRxiv - Neuroscience 2020Quote: ... RT-PCR was performed using GoTaq® qPCR Master Mix (Promega, Shanghai, China) and specific primers (Sangon Biotech ...
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bioRxiv - Microbiology 2019Quote: Reverse transcriptase (RT)-PCR was carried out using ImProm-II reverse transcriptase (Promega) following the manufacturer’s protocol with 1 µg of RNA ...
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bioRxiv - Cell Biology 2022Quote: ... RT-PCR was then performed using the SYBR Green qPCR Master Mix (Promega). Primer sequences are available upon request ...
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bioRxiv - Molecular Biology 2021Quote: ... According to the instructions of the detection kit (Promega), the cells were transfected with the luciferase reporter gene level plasmid ...
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bioRxiv - Genetics 2019Quote: ... using in situ DeadEnd Fluorometric detection kit (G3250-PROMEGA) according to technical protocol (#TB235) ...
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bioRxiv - Microbiology 2020Quote: ... RL activity was measured at the indicated times using a GloMax-Multi Plus Detection System (Promega). The effect of the peptide on membrane fusion activity was evaluated by the DSP assay ...
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bioRxiv - Plant Biology 2023Quote: ... The 16S rDNA PCR-amplified fragments were purified by Wizard® SV Gel and PCR Clean-Up System (Promega, New Delhi, India) and digested by HaeIII (SRL ...
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bioRxiv - Neuroscience 2024Quote: ... BMSCs were transfected with miR-335-3p mimic, miR-335-3p inhibitor and NC that synthesized by RiboBio (Guangzhou, China) via Fugene HD (Promega, Madison, WI, USA; E2311) when cells confluency reached 50%.
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bioRxiv - Microbiology 2022Quote: ... the 16s rRNA gene region was PCR amplified with GoTaq Green Master Mix (Promega, Madison, WI), 16S rRNA gene primers 27F (AGAGTTTGATCMTGGCTCAG ...
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bioRxiv - Genetics 2020Quote: The RNA was isolated using a Maxwell® 16 AS2000 instrument with a Maxwell® 16 LEV simply RNA Tissue Kit (both Promega) following the recommended protocol ...
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bioRxiv - Genetics 2019Quote: Genomic DNAs were isolated from whole blood EDTA samples with a Maxwell 16 instrument using Maxwell 16 LEV Blood DNA kits according to the manufacturer’s protocol (Promega, Madison, WI, USA). These genomic DNAs were used as templates for PCR with a panel of primers that flank the highly polymorphic peptide binding domains encoded by exon 2 of class I (Mamu-A ...
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bioRxiv - Genetics 2022Quote: ... Total RNA was extracted from 14-day-old seedlings (12-days old for the cordycepin experiments) using the Maxwell 16 LEV Plant RNA Kit and Maxwell 16 Instrument (Promega, Madison, WI). For RT-PCR and qRT-PCR ...
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bioRxiv - Microbiology 2021Quote: To clone the 3xant-miR-275 into psiCheck-2 (Promega), two complementary single-stranded oligos that encode 3xant-miR-275 and XhoI and NotI restriction endonuclease cut sites were synthesized at Yale Keck Oligo Synthesis Resource (Table 1) ...
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bioRxiv - Cancer Biology 2022Quote: Efficacy of compounds was validated by applying the real-time CellTox™ Green Cytotoxicity assay (Promega). Assays were performed according to manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2022Quote: ... RNA isolations were performed using Maxwell 16 LEV simplyRNA purification kit (Promega). The silencing of each target transcript was confirmed using qPCR and the appropriate gene-specific primers detailed above (three independent transfections ...
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bioRxiv - Neuroscience 2020Quote: ... RNA was isolated using the Maxwell 16 LEV RNA Isolation Kit (Promega). RNA libraries were prepared by the Genomic Sequencing and Analysis Facility at the University of Texas at Austin and sequenced on the Illumina HiSeq platform ...
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bioRxiv - Molecular Biology 2020Quote: ... total RNA was prepared with Maxwell 16 LEV simplyRNA Tissue kit (Promega).