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Citations for Promega :
151 - 200 of 886 citations for Saporin Goat Polyclonal affinity purified Alexa488 labeled since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2022Quote: ... HaloTag was labeled with tetramethylrhodamine (TMR) by incubating the transfected cells with 2.5 mM HaloLigand-TMR (Promega) for 15 min ...
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bioRxiv - Cell Biology 2020Quote: KNL-2-MBP fragments were [35]S labeled using TnT® Quick Coupled Transcription/Translation System (Promega). 10 μl of the in vitro translation lysate was incubated for 1hr at 4°C with 50 μg CENP-AHCP-3(1-109)-MBP-His (in 20 mM Tris [pH7.5] ...
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bioRxiv - Cell Biology 2021Quote: Proteins labeled with [35S]-methionine were produced by coupled transcription and translation reactions (SP6 or T7, Promega). Translation products were partially purified by ammonium sulfate precipitation (Bardwell & Shah ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... The purified RNA was treated with DNase I (Promega) to remove genomic DNA contaminants and then converted to cDNA using Applied Biosystems High Fidelity RT kit (ThermoFisher) ...
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bioRxiv - Molecular Biology 2021Quote: ... Purified DNA samples were quantified by Quantus (Promega Inc.) Fluorometer™ ...
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bioRxiv - Neuroscience 2021Quote: ... purified with phenol:chloroform:isoamyl alcohol (PCI) and Wizard resin (Promega), digested with XbaI and BamHI-HF in Cutsmart Buffer (NEB) ...
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bioRxiv - Genetics 2020Quote: ... Plasmids were purified with Wizard Plus SV Minipreps (Promega) and sequenced.
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bioRxiv - Genetics 2019Quote: ... purified and cloned into pGEM-T easy vector (Promega). Recombinant clones were then sent to Macrogen (South Korea ...
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bioRxiv - Genomics 2021Quote: ... and purified using SV Total RNA Isolation System (Promega). RNA samples were quantified ...
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bioRxiv - Biochemistry 2021Quote: ... GST-4xSUMO2 was purified using MagneGST Glutathione particles (Promega) as previously described150 ...
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bioRxiv - Microbiology 2022Quote: ... purified using a Wizard Genomic DNA Purification kit (Promega), and sent for Sanger sequencing (ACGT) ...
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bioRxiv - Synthetic Biology 2020Quote: ... and purified with the Wizard SV Gel kit (Promega). The purified DNA was incubated with DpnI (New England BioLabs® ...
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bioRxiv - Molecular Biology 2020Quote: ... and then purified using Magne HaloTag Beads (G7281) (Promega) and Magne HaloTag Beads ...
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bioRxiv - Plant Biology 2022Quote: ... and then purified using Magne HaloTag Beads (G7281) (Promega). The Magne HaloTag Beads and ZmEREB57-HaloTag mixture were incubated with 500 ng DNA library in 40 µL PBS (Phosphate Buffered Saline ...
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bioRxiv - Cell Biology 2022Quote: ... and purified by incubation with GSH-magnetic beads (Promega) after extensive washings ...
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bioRxiv - Microbiology 2023Quote: ... PCR products were purified using PCR cleanup kit (Promega). Sanger sequencing was performed at Psomagen ...
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bioRxiv - Microbiology 2023Quote: ... gel-purified and digested with DpnI restriction endonucleases (Promega) before assembly ...
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bioRxiv - Biochemistry 2020Quote: ... and Alexa Fluor 680 goat anti-mouse antibody (Promega, W402B). Blots were imaged on a Peqlab Fusion SL Vilber Lourmat ...
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bioRxiv - Molecular Biology 2020Quote: ... or goat anti-rabbit IgG at 1:3000 (Promega W4011). Washes were with high-salt TBST (50 mM Tris pH 7.5 400 mM NaCl 0.1% Tween-20).
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bioRxiv - Immunology 2019Quote: ... HRP-conjugated goat anti-mouse IgG (Promega, Madison, WI, USA) were added into each well ...
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bioRxiv - Immunology 2022Quote: ... Goat anti-rabbit IgG conjugated to HRP (1:5,000) (Promega) was used to detect the bound protein ...
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bioRxiv - Immunology 2023Quote: ... goat anti-rabbit IgG conjugated to horseradish peroxidase (HRP) (Promega) was added and incubated for one h at 37°C ...
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bioRxiv - Biochemistry 2022Quote: ... and goat anti-rabbit HRP conjugated antibody (Promega Cat # W4011). Blots were detected using Clarity Western ECL Substrate (Bio-Rad Cat# 1705061 ...
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bioRxiv - Microbiology 2024Quote: ... mNeonGreen and Halotag fusion proteins were detected using polyclonal anti-mNeonGreen (NC, unpublished) and anti-Halotag (Promega) antibodies diluted at 1:10,000 and 1:2,000 respectively ...
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bioRxiv - Genomics 2019Quote: ... 35S labeled proteins were synthesized in vitro using the TNT T7 Quick for PCR DNA kit (Promega, L5540). Labeled proteins were incubated with MBP-tagged proteins in TNEN50 (50mM Tris pH7.5 ...
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Multimodal tubulin binding by the yeast kinesin-8, Kip3, underlies its motility and depolymerizationbioRxiv - Biophysics 2021Quote: ... The protein was fluorescently labeled with 10uM TMR (dimer constructs) or 30uM TMR (monomer constructs, HALO-TMR; Promega) on beads for 1h at 4°C ...
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bioRxiv - Microbiology 2021Quote: ... 35S methionine-labeled full-length cGAS and E5 was generated with the TnT Coupled Reticulocyte Lysate System (Promega) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2021Quote: ... Halo-tagged proteins were labeled by incubating cells with 200nM HaloTag Ligand conjugated to Janelia Fluor 646 (Promega) for 30 min before imaging ...
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bioRxiv - Microbiology 2023Quote: ... The fluorescently labeled PCR products were digested by using the 4-bp cutter TaqI (Promega, Southampton, United Kingdom) as described by the manufacturers ...
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bioRxiv - Neuroscience 2024Quote: ... cells were washed with HBSS and labeled with 100 nM HaloTag NanoBRET 618 ligand (Promega Corp., Fitchburg, WI) in phenol red-free Opti-MEM I reduced serum medium (Gibco/Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2024Quote: ... HaloTag-receptor expressing mESCs were fluorescently labeled with either 1 µM of HaloTag AlexaFluor 488 ligand (Promega, G1001) or 3.5µM of HaloTag AlexaFluor 660 ligand (Promega ...
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bioRxiv - Genomics 2021Quote: ... Barcoded and amplified cDNAs were purified using ProNex beads (Promega), following the workflow targeting transcripts around 2 kb in length ...
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bioRxiv - Cell Biology 2019Quote: ... purified chromatin was recovered using a PCR purification kit (Promega). Sequencing libraries were generated using standard methods and samples were sequenced on a MiniSeq instrument (Illumina ...
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bioRxiv - Zoology 2019Quote: ... purified using Wizard SV Gel and PCR Cleanup kit (Promega), and sequenced directly (Sequetech ...
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bioRxiv - Cell Biology 2019Quote: ... coli and purified with the PureYield Plasmid Miniprep System (Promega). The plasmids were then transfected into HEK293T cells using jetPRIME transfection reagent (Polyplus Transfection ...
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bioRxiv - Developmental Biology 2019Quote: ... purified RNA was retrotranscribed with Promega GoScript Reverse Transcriptase (Promega). Custom SYBR probes were used for quantitative real-time PCR (qPCR ...
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bioRxiv - Molecular Biology 2020Quote: ... Native 80S ribosomes were purified from 200 μl RRL (Promega), which was layered onto 10-30% sucrose density gradients in buffer A (20 mM Tris [pH 7.5] ...
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bioRxiv - Microbiology 2019Quote: ... the His-VqmA was purified using MagneHis Ni-Particles (Promega).
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bioRxiv - Synthetic Biology 2022Quote: ... PCR products were purified prior to quantification via QuantiFluor (Promega) and combined into an equimolar pool ...
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bioRxiv - Genetics 2019Quote: ... Purified PCR products were TA-cloned into pGEM-T (Promega), and sequenced at the McGill/Genome Quebec Innovation Centre ...
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bioRxiv - Molecular Biology 2022Quote: ... purified and cloned into PGEM-T Easy Vector (Promega, USA) using the manufacturer’s protocols ...
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bioRxiv - Molecular Biology 2022Quote: RNA was purified using ReliaPrep™ RNA Miniprep Systems (Promega), according to the manufacturer recommendations ...
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bioRxiv - Molecular Biology 2022Quote: ... His-tagged proteins were purified using Ni-NTA resin (Promega); bound proteins were washed with binding buffer and eluted into elution buffer (4 M urea ...
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bioRxiv - Microbiology 2023Quote: ... Recombinant proteins were purified using MagneHis nickel particles (Promega, WI), then dialyzed against EMSA buffer (50mM Tris-HCl ...
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bioRxiv - Molecular Biology 2023Quote: ... DNA was purified with ProNex size-purification system (NG2001, Promega) with 8:5 beads to sample ratio ...
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bioRxiv - Molecular Biology 2023Quote: ... Extracted RNA was purified using RQ1 RNase-free DNase (Promega) then cDNA generated using M-MLV reverse transcriptase and random primers (Promega) ...
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bioRxiv - Bioengineering 2023Quote: ... several concentrations of purified recombinant HaloTag protein standard (Promega, G4491) were labeled with ligand and run in duplicate to generate a calibration curve ...
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bioRxiv - Developmental Biology 2024Quote: ... and incubated with Anti-Beta-Galactosidase Purified Monoclonal Antibody (Promega), 1:200 in blocking solution for 2 h ...
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bioRxiv - Bioengineering 2024Quote: ... and purified using the PureYield MidiPrep System (Promega, Madison, WI).
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bioRxiv - Synthetic Biology 2021Quote: ... transferred by semi-dry Western Blot onto PVDF membranes visualised with polyclonal antibody and BCIP/NBT (Promega, S3771). Anti-bodies used in this study included a monoclonal α-his-antibody (BioLegend ...