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Citations for Promega :
151 - 200 of 1604 citations for Phthalic Acid Unlabeled 100 Ug Ml In Mtbe since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2024Quote: ... 400 μl cold cell lysis buffer (20 mM HEPES, pH 7.4, 100 mM KCl, 5 mM, MgCl2, 500U/ml RNasin-Plus (Promega), 1x protease inhibitor cocktail (200X ...
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bioRxiv - Cell Biology 2020Quote: ... and 10 μM methionine-free amino acid mixture (Promega). The Click-iT reaction was performed with the Click-&-Go Protein Reaction Buffer Kit (Click Chemistry Tools ...
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bioRxiv - Systems Biology 2019Quote: ... and 50 μM of each amino acid (Promega, #L446A). T7 RNA polymerase (NEBs ...
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bioRxiv - Cell Biology 2021Quote: ... 14 μM amino acid mix w/o methionine (Promega)) ...
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bioRxiv - Neuroscience 2023Quote: ... and stained in Diamond Nucleic Acid Stain (Promega, H1181). All experiments were performed in 8-12 week old animals ...
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bioRxiv - Cell Biology 2020Quote: ... Columns were washed with 90% methanol/100 mM TEAB 10 times prior to on-column digestion with 1 ug Trypsin/Lys-C in 50 mM TEAB (47° C, 2 hours; Cat. #V5073, Promega, Madison, WI). The resulting peptides were eluted with sequential washes of 50 mM TEAB ...
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bioRxiv - Molecular Biology 2023Quote: ... Japan) at 37 C for 2 h before digestion overnight with 1 ug Trypsin/Lys-C mix (V5071, Promega, Madison, WI, USA) at 37 C ...
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bioRxiv - Biophysics 2023Quote: ... They were then diluted 5-fold with aqueous 50 mM TEAB and incubated overnight with Sequencing Grade Modified Trypsin (1 ug in 10 uL of 50 mM TEAB; Promega, Madison, WI). Following this an equal volume of ethyl acetate/trifluoroacetic acid (TFA ...
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bioRxiv - Plant Biology 2021Quote: ... Treatment with Proteinase K was carried out in 500 μl of PK Buffer (100 mM Tris-HCl pH8; 50 mM NaCl; 10 mM EDTA; Proteinase K 4 mg/ml; RNasin PROMEGA) 2 h at 55 °C and 15 min at 95 °C ...
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bioRxiv - Plant Biology 2021Quote: ... Three washes were performed with ChIP Dilution Buffer as in DRIP assays before incubating the IP fraction with Proteinase K for 2 h at 55°C plus 15 min at 95°C in PK Buffer (100 mM Tris-HCl pH8; 50 mM NaCl; 10 mM EDTA; 4 mg/ml Proteinase K; RNasin PROMEGA). We added 2 U DNase I (ThermoFisher ...
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bioRxiv - Molecular Biology 2022Quote: ... a total of 225 μg of protein extract isolated using the aforementioned hypotonic buffer (additionally supplemented with 100 U/ml RNasin Plus RNase Inhibitor (Promega) and 2 U/mL Turbo DNase (Ambion) ...
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bioRxiv - Microbiology 2022Quote: ... which were equilibrated in 100 mM ammonium bicarbonate and digested overnight with 20 µg/mL trypsin (Trypsin Gold, Mass Spectrometry grade; Promega) or 20 µg/mL chymotrypsin prior to LC-MS analysis ...
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bioRxiv - Biochemistry 2023Quote: ... dehydrated with 0.2 mL of 100% acetonitrile and rehydrated with 5 μL of the digestion solution containing 15 μg/mL sequencing-grade trypsin (Promega) in 20 mM aqueous solution of NH4HCO3 ...
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bioRxiv - Cancer Biology 2023Quote: 500 cells/well were seeded in 96-well flat-bottom plates in 100 ml medium and an equal volume of the CellTiter-Glo Luminescent Assay Reagent (#G7571; Promega) added at the indicated time-points ...
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bioRxiv - Neuroscience 2023Quote: ... HEK293 cells were then plated onto 35 mm glass coverslips pre-coated with poly-D-lysine (100 g/mL) and transfected with 300 ng of each plasmid using FuGene transfection reagent (Promega) or Lipofectamine 2000 (ThermoFisher) ...
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bioRxiv - Microbiology 2023Quote: ... transfected cells were treated with human IFN-γ at (100 U/mL for 24 hr) and firefly luciferase activity was determined using the dual luciferase reporter assay system (Promega) on SpectraMax iD3 (Molecular Devices ...
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bioRxiv - Developmental Biology 2024Quote: ... To quantify the activity of reports groups of 5 embryos were collected 8 hours post-injection in triplicates and lysed in 100 mL of lysis buffer (Promega). Reporter expression was quantified with the Nano-Glo Dual-Luciferase Reporter Assay System (Promega ...
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bioRxiv - Microbiology 2023Quote: ... Nucleic acid was recovered using the Maxwell RSC instrument (Promega). After DNAse treatment and elution ...
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bioRxiv - Molecular Biology 2020Quote: ... and then Renilla luciferase activities were measured after the addition of 100 ml of Stop & Glo® Reagent (Luciferase Assay Reagent, Promega). Finally ...
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bioRxiv - Microbiology 2020Quote: ... Virus-mAb-containing media was then aspirated from cells and 100 mL of a 1:4 dilution of Bio-glo (Promega, G7940) in PBS was added to cells ...
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bioRxiv - Molecular Biology 2020Quote: ... cell pellets were lysed in polysome lysis buffer (gradient buffer containing 100 mM KCl, 10 mM MgCl2, 0.1% NP-40, 2 mM DTT, and 40 U/ml RNasin; Promega, Leiden, Netherlands), and onto 17–50% sucrose gradients and ultracentrifuged for 2 h at 40,000 rpm ...
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bioRxiv - Biochemistry 2023Quote: ... The sample was then resuspended in 30 μL of 8M urea in PBS and 30 μL of ProteaseMax surfactant (20 μg/mL in 100 mM ammonium bicarbonate, Promega, V2071) with vortexing ...
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bioRxiv - Biochemistry 2023Quote: ... The sample was then resuspended in 30 μL of 8 M Urea and 30 μL of ProteaseMax surfactant (20 μg/mL in 100 mM ammonium bicarbonate, Promega, V2071).
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bioRxiv - Biochemistry 2023Quote: ... cells were washed with 1 mL of 1X PBS and incubated in 100 μL of 1X Passive Lysis Buffer (Promega E1941) at room temperature for at least 30 minutes ...
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bioRxiv - Biochemistry 2021Quote: ... A 15-amino acid linker and SmBiT subunit (peptide 86, Promega) were attached to the C-terminus of rat Gβ1 ...
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bioRxiv - Immunology 2022Quote: ... the Maxwell Viral Total Nucleic Acid Purification kit (Promega, Madison, WI) was used to isolate viral RNA (vRNA ...
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bioRxiv - Microbiology 2022Quote: ... Nucleic acid extracts were treated using RQ1 RNAse-free DNAse (Promega) according to manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... a complete standard mixture of amino acids (Promega, Medison, WI, USA) was analyzed by CE-MS to obtain MS and MS/MS spectra of the proteinogenic amino acids ...
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bioRxiv - Bioengineering 2023Quote: ... and subsequently stained with Diamond™ Nucleic Acid Dye (Promega, H1181) for 10 minutes ...
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bioRxiv - Molecular Biology 2023Quote: ... 100 nM streptavidin (Promega) was included in the master mix ...
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bioRxiv - Microbiology 2024Quote: ... 100 µl of samples were mixed with 100 µl BacTiter-GloTM reagent (Promega) and incubated for 5 min in the dark ...
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bioRxiv - Molecular Biology 2020Quote: ... 150 mM NaCl, 1 mM MgCl2, 2 mM EDTA, 1% NP-40, 1 mM DTT, 100 U/mL RNasin [Promega], 1X protease inhibitor) at 7 dpi ...
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bioRxiv - Microbiology 2020Quote: ... Cells were lysed 48 hr post-transfection in co-IP buffer supplemented with both protease inhibitors and RNAsin (Promega N2515, 100 units/mL). Lysates were processed and immunoprecipitations were performed as described above for the polymerase formation assay ...
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bioRxiv - Developmental Biology 2021Quote: ... cells were transfected pCAG-Lgr6 or control plasmid (100 ng/ml or as noted) using the Fugene HD transfection reagent (Promega, Madison, WI, USA). Subsequent cell culturing and treatment is as noted for individual assays ...
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bioRxiv - Molecular Biology 2021Quote: ... 2mM Mg(OAc)2 and 10μM of the amino acid mixture (Promega). Reactions were incubated with 5μM of the indicated nsp1 protein for 30 min on ice ...
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bioRxiv - Neuroscience 2020Quote: ... samples containing 100 μg total protein were mixed with 100 μl Luciferase Substrate (Promega). The kinetics of the luminescence was recorded for 10 min using a TriStar2 S LB 942 Plate Reader (Berthold Technologies ...
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bioRxiv - Microbiology 2024Quote: ... Briefly, 100 µl elution buffer 1 (100 mM ammonium bicarbonate, 1 µg trypsin (Promega)) was added to each sample ...
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bioRxiv - Cancer Biology 2023Quote: ... 20 µl of lysate was added to a 1.5 ml Eppendorf tubes containing 100 µl of luciferase assay reagent II (Promega luciferase assay system, Cat# E1500) and the mixture pipetted in and out three times ...
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bioRxiv - Developmental Biology 2021Quote: α-βgal (1:100, Promega, Z3781) was used to visualize rP298.lacZ ...
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bioRxiv - Bioengineering 2020Quote: ... Trypsin gold (100 nM; Promega) was added to each sample and proteolysis proceeded for 2 h at 37 °C ...
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bioRxiv - Neuroscience 2022Quote: ... and RNasin (Promega, 1:100) on ice gently ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... and trypsin (100 ng; Promega). We mixed the resulting peptides with a MALDI matrix consisting of an aqueous 50% acetonitrile/0.1% TFA solution of α-cyano-4-hydroxycinnamic acid (5 mg/ml ...
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bioRxiv - Neuroscience 2021Quote: ... 100 ng Random Primers (Promega), 400 ng Anchored Oligo (dT)20 Primer (Invitrogen) ...
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bioRxiv - Molecular Biology 2023Quote: ... and 1:100 RNasin (Promega). Cells were then indirectly fluorescently immunolabeled against Somatostatin and Insulin and sorted ...
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bioRxiv - Cell Biology 2024Quote: ... and 100 μM luciferin (Promega). Chondrocytes were incubated in the presence of 100 nM of dexamethasone for 60-min prior to the media change ...
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bioRxiv - Cell Biology 2024Quote: ... and 100 μM luciferin (Promega). Samples were maintained in 35 mm dishes sealed with 40 mm glass cover slips (Thermo Fisher Scientific ...
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bioRxiv - Plant Biology 2021Quote: The optimised HaloTag®-7 sequence (298 amino acids) from Promega (https://www.promega.de/) was genetically split on position 155/156 aa into the N-terminal fragment “NHalo” (aa 1-155 ...
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bioRxiv - Developmental Biology 2023Quote: ... followed by DNA staining with Diamond™ Nucleic Acid Dye (Promega, Madison, WI)
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bioRxiv - Neuroscience 2019Quote: ... 100 µg of protein was then added to 100 µl of prewashed HaloLink resin (Promega G1914), which was prepared by washing and pelleting for 2m at 800xg 3x in wash buffer (100 mM Tris pH 7.5 ...
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bioRxiv - Molecular Biology 2021Quote: ... and 100 bp DNA ladder (Promega). Clones with an XbaI restriction site were then deep sequenced to confirm correct editing and zygosity ...