Labshake search
Citations for Promega :
151 - 200 of 403 citations for LDH A Human His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Immunology 2021Quote: Cell death was assessed by measuring lactate dehydrogenase (LDH) release into the supernatant using a CytoTox 96 Non-Radioactive Cytotoxicity Assay (Promega) according to the manufacturer’s instructions ...
-
bioRxiv - Immunology 2020Quote: The ability of T cells to lyse organoids was also measured using LDH-Glo™ Cytotoxicity Assay Kit (Promega, USA) according to the manufacturer’s instructions ...
-
bioRxiv - Cell Biology 2023Quote: ... diluted 1:100 in storage buffer and analyzed as indicated in the manufacturer’s protocol (LDH-Glo Cytotoxicity Assay, Promega, #J2381).
-
bioRxiv - Cell Biology 2022Quote: ... Lactate dehydrogenase (LDH) release was measured using the Cyto Tox 96 © Non-Radioactive Cytotoxicity Assay (Promega, Madison, Wisconsin, USA) according to manufacturer’s instructions ...
-
bioRxiv - Microbiology 2023Quote: ... and then collected and used to quantify lactate dehydrogenase (LDH) release (in a 50 μl aliquot) using the CytoTox 96 Non-Radioactive Cytotoxicity Assay kit (Cat No. G1780, Promega) according to the manufacturer’s protocol ...
-
bioRxiv - Biochemistry 2023Quote: ... Cells were incubated for 24hr at 37°C and cell death was measured through a lactate dehydrogenase (LDH) assay using the CytoTox 96 kit (Promega). 50 μL of media was collected from all experimental wells with two replicates each and placed in a fresh 96-well plate ...
-
bioRxiv - Physiology 2023Quote: In vitro cytotoxicity was determined by measuring lactate dehydrogenase (LDH) release upon cell lysis according to the manufacturer’s instructions (Promega, G1780). In brief ...
-
bioRxiv - Immunology 2023Quote: ... The supernatants were used to determine lactate dehydrogenase activity and IL-1β level using LDH-GloTM Cytotoxicity Assay (Promega, J2380) and Mouse IL-1 beta DuoSet ELISA (R&D Systems ...
-
bioRxiv - Cancer Biology 2023Quote: ... Target and effector cells were cultured 4 hours or overnight and cytotoxicity was measured using the CytoTox96 LDH assay (Promega) or using click beetle green luciferase expressing cells and read on a BioTek Synergy H1 plate reader (BioTek Instruments) ...
-
bioRxiv - Microbiology 2024Quote: ... The presence of released LDH in the supernatant was assessed using the Cytotox 96 Non-Radioactive Cytotoxicity Assay (Promega, G1780). The extended protocol can be found in the supplementary materials.
-
bioRxiv - Microbiology 2024Quote: Lytic cell death was indirectly studied by measuring lactate dehydrogenase (LDH) release in cell culture medium using the CytoTox 96 nonradioactive cytotoxicity assay kit (G1780, Promega) according to the manufacturer’s instructions ...
-
bioRxiv - Neuroscience 2021Quote: Cell cytotoxicity was detected by measuring lactate dehydrogenase (LDH) levels using the CytoTox 96® Non-Radioactive Cytotoxicity Assay (Promega, G1780). The manufacturer’s instructions were followed and the absorbance reflecting the LDH content in the cell supernatant was measured at 492 nm (600 nm reference ...
-
bioRxiv - Immunology 2021Quote: ... Cell supernatants were analyzed for LDH activity using the CytoTox 96® Non-Radioactive Cytotoxicity Assay Kit (Promega, Cat. no: G1780).
-
bioRxiv - Biophysics 2021Quote: ... We detected the cytotoxicity by a lactate dehydrogenase (LDH) assay using the Cytotox 96 non-radioactive cytotoxicity assay kit (Promega, USA) according to the kit instructions ...
-
bioRxiv - Neuroscience 2020Quote: 50 μl supernatant were collected as designed time point into a 96-well plate for lactate dehydrogenase (LDH) release assay as manufacture’s protocol (Promega, cat# G1780). Briefly ...
-
bioRxiv - Immunology 2019Quote: ... Cytotoxicity was then measured from cell supernatants based on release of lactate dehydrogenase (LDH) released from dying cells based on the CytoTox 96® Non-Radioactive Cytotoxicity assay from Promega and done according to manufacture protocol ...
-
bioRxiv - Pathology 2019Quote: ... Lactate Dehydrogenase (LDH) release was measured regularly (twice per week) using a CytoTox 96® Non-Radioactive Cytotoxicity Assay kit (Promega).
-
bioRxiv - Microbiology 2021Quote: ... cell viability was determined by measuring LDH release using the CellTiter 96® Aqueous Non-Radioactive Cell Proliferation Assay (Promega, MI) as per manufacturer’s instructions ...
-
bioRxiv - Neuroscience 2020Quote: Cell cytotoxicity was detected by measuring lactate dehydrogenase (LDH) levels using the CytoTox 96® Non-Radioactive Cytotoxicity Assay (Promega, G1780). The manufacturer’s instructions were followed and the absorbance reflecting the LDH content in the cell supernatant was measured at 492 nm (600 nm reference ...
-
bioRxiv - Immunology 2021Quote: The cytotoxicity of drugs on the cultured cells was measured by the release of LDH (an indicator of cytotoxicity) using a CytoTox 96 Non-Radioactive Cytotoxicity Assay (Promega, G1780) following the provided technical bulletin.
-
bioRxiv - Cancer Biology 2022Quote: ... by quantifying the activity of lactate dehydrogenase (LDH) released into the supernatant using a colorimetric assay (CytoTox 96® Non-Radioactive Cytotoxicity Assay kit from Promega) and following the manufacturer’s instructions.
-
bioRxiv - Neuroscience 2023Quote: Cytotoxicity was assessed by measuring the lactate dehydrogenase (LDH) release from cells with damaged membranes using the CytoTox-ONE Homogeneous Membrane Integrity assay (#7891, Promega, USA). For this ...
-
bioRxiv - Neuroscience 2023Quote: Cell death was evaluated in 30 day-old non-embedded organoids using LDH-Glo™ Cytotoxicity Assay (Promega, cat. no J2380) accordingly to the manufacturer’ instructions ...
-
bioRxiv - Microbiology 2023Quote: ... LDH was measured in 13h-culture supernatants using CytoTox 96 Non-Radioactive cytotoxicity assay according to manufacturer instructions (Promega, WI, USA).
-
bioRxiv - Physiology 2024Quote: ... The toxicity of MNP was determined 24 h upon MNP treatment using the LDH-toxicity Assay (Promega Corp, Fitchburgh, WI, US) according to the manufacturer’s instructions ...
-
bioRxiv - Immunology 2024Quote: ... 18-24 hours later the supernatant was harvested for cytotoxicity evaluation by measuring LDH release with CytoTox 96 nonradioactive cytotoxicity assay kit (Promega, #G1780), followed the protocol suggested by the manufacturer.
-
bioRxiv - Microbiology 2024Quote: ... Treatments did not affect bacterial or cell viability as assessed by Lactate dehydrogenase release in the culture supernatant (LDH cytotoxicity kit®, Promega, according to the manufacturer’s instructions ...
-
bioRxiv - Synthetic Biology 2023Quote: ... His-tag proteins have been purified by using MagneHis system (Promega) and DynaMag spin magnet (Thermo Fisher Scientific) ...
-
bioRxiv - Cell Biology 2021Quote: ... Cell death was determined according to the activity of lactate dehydrogenase (LDH) in the culture supernatants using a CytoTox® Kit (Promega, USA) according to the manufacturer’s instructions.
-
bioRxiv - Cell Biology 2021Quote: ... Cell death of infected cardiomyocytes was determined according to the activity of lactate dehydrogenase (LDH) in the culture supernatants using a CytoTox® Kit (Promega, USA) according to the manufacturer’s instructions ...
-
bioRxiv - Neuroscience 2021Quote: ... Cytotoxicity was determined according to the activity of lactate dehydrogenase (LDH) in the culture supernatants using a CytoTox® Kit (Promega, USA) according to the manufacturer’s instructions ...
-
bioRxiv - Neuroscience 2022Quote: The CytoTox 96 Non-Radioactive Cytotoxicity Assay or LDH-Glo Cytotoxicity Assay was performed as per manufacturer’s instructions using 50 uL conditioned media replicates to measure lactate dehydrogenase (LDH) release (Promega cat# G1780, J2380). For the CytoTox 96 assay 490 nm absorbance readings were taken on a SpectraMaxM5plate reader with SoftMax Pro 7.1 software and data is presented as 490 nm absorbance ...
-
bioRxiv - Immunology 2022Quote: Cytotoxicity was determined by the lactate dehydrogenase (LDH) release-based assay using the CytoTox 96® non-radioactive cytotoxicity assay kit (Promega Corporation) as previously described.11 Live-cell imaging assays were performed using the IncuCyte platform (Essen BioScience) ...
-
bioRxiv - Cancer Biology 2023Quote: T cell cytotoxicity induced by BiTEs and BiTE-sialidase fusion proteins was measured by lactate dehydrogenase (LDH) release using CytoTox 96® Non-Radioactive Cytotoxicity Assay (Promega, G1780). 10000 tumor cells and 50000 hPBMCs per well in 100 μL media were exposed to different treatments and incubated in 96 well plates at 37 °C (unless different ratio was specified elsewhere) ...
-
bioRxiv - Immunology 2024Quote: ... AP20187 dimerizer was added in cell culture media to a final concentration of 500 nM for 5 hours before taking the supernatants for LDH assay (G1780, Promega, Madison, WI). Cell death was measured on a scale with the media without cells set to 0% and cells lysed for 1 hour in 1% Triton X-100 set to 100%.
-
bioRxiv - Cell Biology 2024Quote: ... the supernatant was measured for LDH (Lactate dehydrogenase) release using the kit of CytoTox 96® Non-Radioactive Cytotoxicity Assay (Promega, G1780), according to the manufacturer’s instructions.
-
bioRxiv - Genomics 2020Quote: ... At the time of analysis samples were diluted with an equal volume of LDH Detection Reagent and equilibrated for 60 minutes at room temperature before reading the luminescence (GloMax 96 Microplate Luminometer, Promega Corp, Madison, WI) in relative luminescence units (RLU) ...
-
bioRxiv - Bioengineering 2021Quote: ... At time points of 1 h and 48 h samples were tested for cytotoxicity via a commercially available LDH-Glo™ Cytotoxicity Assay (#J2380, Promega, Madison, WI). In brief ...
-
bioRxiv - Microbiology 2021Quote: Apical and basolateral samples were used to quantify LDH release in RV-C15-infected HAE with the CytoTox 96® Non-Radioactive Cytotoxicity Assay kit (#G1780; Promega, Madison, WI) following the manufacturer’s protocol.
-
bioRxiv - Microbiology 2021Quote: LDH activity and percentage cell cytotoxicity in rabbit lungs (n=3 per group per time point) were determined using the LDH-Glow cytotoxicity kit as per the manufacturer’s instructions (Promega Corporation, Madison, WI, USA). Luminescence was recorded using a GloMax plate reader ...
-
bioRxiv - Pharmacology and Toxicology 2023Quote: ... membrane integrity (CytoTox-OneTM homogeneous membrane integrity assay kit: lactate dehydrogenase (LDH release) and caspase activity/ apoptosis (Caspase-Glo® 3/7 assay system kit) (all from Promega, WI, USA), as described previously17 (see Supplementary data for detailed description).
-
bioRxiv - Biochemistry 2021Quote: ... The 3xFlag-m-PKD1-2xMyc/His insert was subcloned into pCI vector (Promega). To produce CTF expression constructs of human (h ...
-
bioRxiv - Bioengineering 2021Quote: ... we measured the release of lactate dehydrogenase (LDH) into the media effluents using the CytoTox 96® Non-Radioactive Cytotoxicity Assay (G1780, Promega GmbH, Walldorf, Germany). The assay was performed in a 384-well plate according to the manufacturer’s instructions ...
-
bioRxiv - Genomics 2021Quote: ... thawed samples were diluted with an equal volume of LDH Detection Reagent and equilibrated for 60 minutes at room temperature before reading the luminescence (GloMax 96 Microplate Luminometer, Promega Corp Madison, WI USA) in relative luminescence units (RLU) ...
-
bioRxiv - Microbiology 2023Quote: ... Cytotoxicity was evaluated by quantification of lactate dehydrogenase (LDH) release in culture supernatant of transfected-cells using CytoTox96 nonradioactive cytotoxicity assay (Promega, Charbonnières-les-Bains, France) according to the manufacturer’s instructions ...
-
bioRxiv - Microbiology 2024Quote: ... Cytotoxicity was evaluated by quantification of lactate dehydrogenase (LDH) release in cell cultures using CytoTox 96 nonradioactive cytotoxicity assay (Promega, Charbonnières-les-Bains, France) according to the manufacturer’s instructions ...
-
bioRxiv - Molecular Biology 2024Quote: ... stored at 4°C and assayed the following day for lactate dehydrogenase (LDH) activity as a measure of cell death using a Cytotox 96® Non-radioactive Cytotoxicity Assay (Promega, Madison, Wisconsin, USA).
-
bioRxiv - Systems Biology 2020Quote: ... Human cell lysate (MS Compatible Human Protein Extract, Digest, V6951) were purchased from Promega.
-
bioRxiv - Neuroscience 2020Quote: ... All designed probes were tested by ddPCR using human genomic DNA (Human male, Promega) as a negative control ...
-
bioRxiv - Molecular Biology 2022Quote: ... Human Genomic DNA (G1521, Promega) was used as positive control and synthetic DNA was used to validate the LAMP assay performance ...