Labshake search
Citations for Promega :
151 - 200 of 4438 citations for 6H Purin 6 one 2 amino 8 chloro 1 9 dihydro since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Cell Biology 2023Quote: ... 30 μL of CellTiter-Glo reagent solution (Promega, diluted 1:6 in water) was added to each well after 24 h and 72 h of compound treatment ...
-
bioRxiv - Microbiology 2023Quote: The genes were cloned one by one into in pGEM-T Easy Vector (Promega, Madison, USA) and finally in pMG36e vector (Addgene ...
-
bioRxiv - Microbiology 2021Quote: ... and the SARS-CoV-2 spike protein (23) into HEK 293T cells using the FuGENE 6 Transfection Reagent (Promega). Spike sequences from the following global strains REF were used ...
-
bioRxiv - Cell Biology 2019Quote: ... Cells were transfected with 2 μg of FLAG-tagged β2 or β1 adrenergic receptor DNA using Fugene 6 (Promega) and seeded at a density of ∼70,000 cells.ml−1 on 18 mm glass coverslips 4-8 hours after transfection.
-
bioRxiv - Cell Biology 2022Quote: ... were transfected with 2 μg of fresh bacmid DNA and 6 μL of FuGene HD transfection reagent (Promega, E2311) according to the manufacture’s instruction ...
-
bioRxiv - Synthetic Biology 2022Quote: ... Peptide digestion was initiated by adding 25 μl of 50 mM ammonium bicarbonate buffer (pH 8) containing 2 μg trypsin (Sequencing Grade Modified Trypsin, Promega #V5117) directly on top of the column and incubating overnight at 37 °C in a drying oven ...
-
The human sperm basal body is a complex centrosome important for embryo pre-implantation developmentbioRxiv - Developmental Biology 2021Quote: ... The resulting protein extract was first diluted to 2M urea for overnight digestion with LysC (Wako, USA) at 37°C and then diluted 2-fold for 8 h digestion with trypsin (Promega, USA) at 37°C.
-
bioRxiv - Neuroscience 2024Quote: ... Peptide digestion was initiated by adding 25 µL of 50 mM ammonium bicarbonate buffer (pH 8) containing 2 µg trypsin (Sequencing Grade Modified Trypsin, Promega, # V5117) directly on top of the column and incubating overnight at 37 °C ...
-
bioRxiv - Cell Biology 2020Quote: ... washed again in DPBS and stained with 1mg/ml X-gal (5-bromo-4-chloro-3-indolyl-β-galactopyranoside, Promega, Madison, WI, US) in DMF (Dimetil formamide ...
-
bioRxiv - Microbiology 2023Quote: ... and the reaction was revealed with a solution of NBT (nitroblue tetrazolium) and BCIP (5-bromo-4-chloro-3-indolyl-phosphate) (Promega, Madison, WI, USA).
-
bioRxiv - Microbiology 2020Quote: ... or Vpr F34I/P35N mutant using 6 μl Fugene-6 (Promega). For KPNA-cargo IPs HEK293T cells were grown in 10 cm dishes and co-transfected with 1 μg of a plasmid expressing FLAG-tagged KPNA1 ...
-
bioRxiv - Cancer Biology 2021Quote: ... MTS reagent (CellTiter96 AQueous One; Promega) was added and the optical density was read at 490 nm to assess viability.
-
bioRxiv - Biochemistry 2019Quote: ... Luciferase reagent (Promega One-Glo, E6120) was added to cells after compound treatment and incubated for 10 minutes according to manufacturer recommendations ...
-
bioRxiv - Systems Biology 2020Quote: ... ONE-GloTM Luciferase Assay System (Promega) and Renilla-Glo® Luciferase Assay System (Promega ...
-
bioRxiv - Molecular Biology 2019Quote: ... using QuantiFluor ONE dsDNA kit (Promega) and library size was checked on the Bioanalyzer (Agilent Technologies ...
-
bioRxiv - Molecular Biology 2022Quote: ... using QuantiFluor ONE Lambda DNA (Promega) as a DNA standard ...
-
bioRxiv - Molecular Biology 2022Quote: ... or QuantiFluor ONE dsDNA System (Promega) using QuantiFluor ONE Lambda DNA (Promega ...
-
bioRxiv - Evolutionary Biology 2023Quote: ... one volume of CellTiter-Glo (Promega) assay reagent was added to each well ...
-
bioRxiv - Microbiology 2023Quote: One step RT-PCR kit (Promega) was used ...
-
bioRxiv - Genetics 2023Quote: ... One microgram of Trypsin Gold (Promega) was added to each sample and incubated in an end-over-end mixer at 37°C for 16 hours ...
-
bioRxiv - Developmental Biology 2023Quote: ... The QauntiFluor ONE dsDNA System (Promega) is used to quantify the double stranded library ...
-
bioRxiv - Cell Biology 2023Quote: ... 2.5 units of RNase ONE (Promega) was added to the sample ...
-
bioRxiv - Immunology 2024Quote: ... 40 μL of One-GloEX (Promega) was added to the cells and incubated in the dark for 5 min prior to reading on an Agilent BioTek Neo2 plate reader ...
-
bioRxiv - Biochemistry 2024Quote: ... 40 μL of One-GloEX (Promega) was added to the cells and incubated in the dark for 5 min prior to reading on an Agilent BioTek Neo2 plate reader ...
-
bioRxiv - Molecular Biology 2021Quote: ... One hundred nanograms of genomic DNA spiked with 1% (w/w) of unmethylated lambda DNA (Promega) was used for the library preparation ...
-
bioRxiv - Molecular Biology 2023Quote: ... One-step RT-qPCR was performed using the GoTaq 1-Step RT-qPCR System (Promega, #A6020) and MicroAmp Fast Optical 96 Well Reaction Plates (Applied Biosystems ...
-
bioRxiv - Zoology 2024Quote: One-step RT-qPCR was conducted using GoTaq 1-Step RT-qPCR System kit (Promega, France) in total reaction volume of 20 µl containing 2 µl of RNA extracts and 300 nM of the same forward and reverse primers as above ...
-
bioRxiv - Microbiology 2022Quote: ... the virus-induced cytopathogenic effect was measured colorimetrically by the formazan-based 3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium (MTS) cell viability assay (CellTiter 96 AQueous One Solution Cell Proliferation Assay from Promega, Madison, WI), and the antiviral activity was expressed as the 50% effective concentration (EC50) ...
-
bioRxiv - Cell Biology 2019Quote: ... Caspase 8 activity was measured by using the Caspase Glo 8 assay system (Promega, G8202) according to the manufacturer’s protocol.
-
bioRxiv - Molecular Biology 2020Quote: ... [9] with minor modifications including the use of Magnetic Halolink beads (Promega). The PUM test proteins remained covalently bound via N-terminal Halotag to the beads.
-
bioRxiv - Biochemistry 2021Quote: ... A 15-amino acid linker and SmBiT subunit (peptide 86, Promega) were attached to the C-terminus of rat Gβ1 ...
-
bioRxiv - Microbiology 2023Quote: ... a complete standard mixture of amino acids (Promega, Medison, WI, USA) was analyzed by CE-MS to obtain MS and MS/MS spectra of the proteinogenic amino acids ...
-
bioRxiv - Cell Biology 2019Quote: Glucose uptake was measured based on the detection of 2-deoxyglucose-6-phosphate uptake by a commercially available luminescence-based kit (Glucose Uptake-GloTM Assay, Promega) on a SpectraMax M3 (Molecular Devices) ...
-
bioRxiv - Cell Biology 2019Quote: ... HEK293T cells were grown in two 175 cm2 flasks to ∼50% confluence and transfected with 25 μg plasmid and 75 μl FuGENE 6 in 2 ml Opti-MEM according to the manufacturers instructions (Promega). One day after transfection ...
-
bioRxiv - Biochemistry 2021Quote: ... HEK293T cells and C3H10T1/2 cells were transfected in 6-well plates with various constructs of pmirGlo vector (Promega®). Transfection was performed using Turbofect transfection reagent (Thermo Fischer scientific® ...
-
bioRxiv - Biochemistry 2021Quote: ... HEK293T cells were grown to 80% confluency before transfection with pCMV3-SARS-CoV-2-spike (kindly provided by Dr. Peihui Wang, Shandong University, China) using FuGENE 6 (Promega). Cells were cultured overnight at 37 °C with 5% CO2 ...
-
bioRxiv - Cell Biology 2019Quote: Desired protein amounts were aliquoted and chloroform methanol precipitated, followed by digestion with LysC (overnight at room temperature, vortex speed 2; Wako) and trypsin (6 hours, 37° Promega) digestion ...
-
bioRxiv - Molecular Biology 2021Quote: A total of 10 μg of bacmid DNA was transfected into 6 wells of 2×106 adherent Sf9 cells (at 5×105 cells/ml) using the transfection reagent Fugene HD (Promega). 42–72 h post transfection ...
-
bioRxiv - Immunology 2021Quote: ... HEK293T cells were grown to 80% confluency before transfection with pCMV3-SARS-CoV-2-spike (kindly provided by Dr. Peihui Wang, Shandong University, China) using FuGENE 6 (Promega). Cells were cultured overnight at 37°C with 5% CO2 ...
-
bioRxiv - Biochemistry 2023Quote: ... 1 × 106 Sf9 cells in 2 mL of media in a 6-well dish were transfected with up to 2 µg of fresh bacmid DNA using FuGene HD transfection reagent (Promega) at a ratio of 3:1 (Fugene reagent:DNA ...
-
bioRxiv - Immunology 2023Quote: ... and the corresponding SARS-CoV-2 spike construct were co-transfected in HEK293-T cells using FuGENE 6 Transfection Reagent (Promega) in Dulbecco’s Modified Eagle Medium (DMEM ...
-
bioRxiv - Microbiology 2020Quote: ... HeLa cells were transfected 6 hours before imaging with Fugene 6 (Promega) and 1000ng of DNA ...
-
bioRxiv - Cell Biology 2021Quote: ... 1 μg of both plasmids was mixed with 6 μl FuGENE reagent (Promega E269A) in 200 μl serum free DMEM and incubated for 30 minutes before adding the transfection mix to the cells ...
-
bioRxiv - Cell Biology 2023Quote: ... 1 μg of both plasmids was mixed with 6 μl FuGENE reagent (Promega E269A) in 200 μl serum free DMEM and incubated for 30 min before adding the transfection mix to the cells ...
-
bioRxiv - Molecular Biology 2023Quote: ... diluted with 4 volumes of 20 mM Tris-HCl pH 8 2 mM CaCl2 prior to overnight digestion with 100 ng sequencing grade trypsin (Promega, Cat# V5111) at room temperature ...
-
bioRxiv - Cell Biology 2020Quote: ... using Fugene 6 (Promega). Supernatant was harvested from the transfected cells for the next three days and then centrifuged ...
-
bioRxiv - Cell Biology 2020Quote: ... using Fugene 6 (Promega), with a linearized TCIS construct described previously6 ...
-
bioRxiv - Genetics 2021Quote: ... using FuGENE 6 (Promega) following manufacturer’s instructions ...
-
bioRxiv - Microbiology 2020Quote: ... using FuGENE 6 (Promega). Sixteen hours later ...
-
bioRxiv - Cancer Biology 2020Quote: Transfection: Fugene 6 (Promega); TransIT-2020 (Mirus Bio) ...