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Citations for Promega :
1901 - 1950 of 6132 citations for Cortisol ELISA Kit 1 Strip plate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2022Quote: ... Beads were washed three times for 10 min with gentle rotation in high-salt buffer (50 mM Tris, pH 7.5, 300 mM KCl, 12 mM MgCl2, 1% Nonidet P-40, 1 mM DTT, 100U/mL Promega RNasin ...
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bioRxiv - Cell Biology 2022Quote: ... polysome were stabilised by washing cells in 20 ml ice-cold water containing 0.1 mg/ml CHX before being resuspended in 1 ml RiboTag Lysis Buffer (50 mM Tris, pH 7.5, 100 mM KCl, 12 mM MgCl2, 1% Nonidet P-40, 1 mM DTT, 100U/mL Promega RNasin ...
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bioRxiv - Biophysics 2020Quote: ... All samples were brought to room temperature and mixed with a 1:1 volume of nGlow solution (Promega, #N1110). Samples (90% of total volume ...
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bioRxiv - Cancer Biology 2021Quote: ... the samples were diluted to 1 M Urea with 10 mM EPPS pH 8.2 and incubated overnight with 1:50 LysC (Wako Chemicals) and 1:100 Sequencing grade trypsin (Promega). Digests were acidified using TFA and tryptic peptides were purified by Oasis Prime HLB columns (30 mg ...
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bioRxiv - Biophysics 2021Quote: ... All samples were brought to room temperature and mixed with a 1:1 volume of nGlow solution (Promega, #N1110). Samples (90% of total volume ...
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bioRxiv - Developmental Biology 2022Quote: HEK293T cells were simultaneously transfected with 1 μg pCS2Cachd1-eGFP or pCS2Cachd1_V1122D-eGFP and 1 μg KDEL-tRFP plasmid using Fugene 6 transfection reagent (Promega). eGFP/tRFP expression was confirmed 24 hours post transfection and the cells fixed at 42 hours post transfection and imaged on a spinning disk microscope (see below).
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bioRxiv - Molecular Biology 2019Quote: ... Cell pellets were lysed in 300µl of Lysis Buffer (50mM Tris-HCl pH 7.5, 150mM NaCl, 1% Triton® X-100, 0.1% deoxycholate, Protease Inhibitor and RNasin Plus [1:500, Promega]),diluted to a final concentration of ~1µg/µl with 100mM Tris-HCl pH 7.5 ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... Cell media was then replaced with a 1:1 mixture of fresh media and Nano-Glo Luciferase reagent (Promega) and luminescence was measured using a SpectroMax M5e (Molecular Devices) ...
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bioRxiv - Immunology 2021Quote: ... The media from infected cells was then removed and 100 μl of 1:1 diluted PBS:Bio-Glo (Promega, G7940) luciferase substrate was added to each well ...
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bioRxiv - Cancer Biology 2020Quote: ... the samples were diluted to 1 M Urea with 10mM EPPS pH 8.2 and incubated overnight with 1:50 LysC (Wako Chemicals) and 1:100 Sequencing grade trypsin (Promega). Digests were acidified using TFA and tryptic peptideswere purified by tC18 SepPak (50 mg ...
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bioRxiv - Microbiology 2021Quote: ... the cells were lysed with a 1:1 ratio of virus to Bright-Glo luciferase assay media (Promega, #E2610) and the contents were analyzed on a luminometer (LUMISTAR Omega ...
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bioRxiv - Microbiology 2022Quote: ... cells were lysed by adding 100 µl of 1:1 (v:v) mixture of phenol red-free DMEM and Bright-Glo substrate (Promega) and luminescence detected using a Glomax Navigator (Promega) ...
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bioRxiv - Molecular Biology 2022Quote: ... 1 mM DTT) in presence of 1 mM Adenylyl-imidodiphosphate (AMP-PNP) and RNase inhibitor RNAsin (0.5 U, Promega) for 25 min at 4°C with head-over-end rotation ...
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bioRxiv - Immunology 2022Quote: ... caspase-1 activity was quantified in supernatants using a commercially available assay (Caspase-GLO 1 Assay; Promega, Madison, WI) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2023Quote: ... Media were removed and 100 μl of CTG reagent (CellTiter-Glo®, Promega GmbH, Germany; 1:1 with PBS) per well was added ...
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bioRxiv - Microbiology 2023Quote: ... CM was then harvested by centrifugation and active caspase-1 measured with the Caspase-Glo 1 Inflammasome Assay (Promega) following the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2023Quote: ... Sample C was treated with 1% Triton X-100 (TX100) followed by treatment with 1μg mL-1 trypsin (Promega). For the detergent treatment ...
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bioRxiv - Bioengineering 2023Quote: ... The reaction was initiated by the addition of 1-250 nM acylated peptide (Vivitide) and a 1:10,000 dilution of LgBiT subunit of the NanoBiT luciferase (Promega) and read using an Envision plate reader (XCite 2105 ...
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bioRxiv - Genomics 2022Quote: ... Nuclei were resuspended in 200 μL resuspension buffer (1x PBS, 1% BSA and 0.2U μl/1 RNasin Plus RNase Inhibitor (Promega)) and transferred to a 1.5 mL Eppendorf tube tube ...
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bioRxiv - Neuroscience 2023Quote: ... and Dounce homogenized in 500 µL of ice-cold RNase-free buffer polysome buffer (50 mM Tris pH 7.5, 100 mM KCl, 12 mM MgCl2, 1% NP-40, 1 mM DTT, 200U/ml Promega RNasin ...
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bioRxiv - Developmental Biology 2023Quote: ... a minimum of 1 µg of RNA was combined with 1 µl of Oligo (dT)15 primer (Promega, C110B) and nuclease-free water to make up a total of 5 µl and incubated at 70°C and 4°C for 5 min each ...
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bioRxiv - Developmental Biology 2023Quote: ... A minimum of 1 µg of RNA was combined with 1 µl of Oligo (dT)15 primer (Promega, C110B) and nuclease-free water to make up a total of 5 µl and incubated at 70°C and 4°C for 5 min each ...
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bioRxiv - Molecular Biology 2023Quote: ... Two μL of reverse transcription buffer 5X were added and the samples were incubated at 20°C for 15 min before addition of 1.5 μL of dNTP mix (1 mM of dATP, dGTP, dCTP, dUTP) and 1 μL of AMV enzyme (PROMEGA). Reverse transcription was performed at 42°C for 45 min and synthesized cDNA was recovered by ethanol precipitation as described above ...
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bioRxiv - Molecular Biology 2023Quote: ... The secondary antibody was a horseradish peroxidase polyclonal goat anti-mouse (from 1:5,000 to 1:10,000, depending on the primary antibody; Promega, W4021). Proteins were detected with the ECL chemiluminescence reagent (GE Healthcare ...
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bioRxiv - Biophysics 2023Quote: ... we stained the cells with 1 ml of a 1:2,000 TMR Halo-ligand solution (Promega, Cat. No.: G8251) to fluorescently label the Halo-tagged proteins ...
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bioRxiv - Molecular Biology 2024Quote: ... cells were incubated with the primary antibodies (1D4 1:1500, Anti-Calnexin Sigma c4731 1:600, Anti-LgBiT Promega N7100 1:100 ...
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Bacteria Are a Major Determinant of Orsay Virus Transmission and Infection in Caenorhabditis elegansbioRxiv - Microbiology 2024Quote: ... Orsay Virus RNA1 level was quantified by qPCR using 1 μl of 1/5 diluted cDNA (GoTaq Promega A6001) and run on QuantStudio 3 Real Time PCR system ...
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Bacteria Are a Major Determinant of Orsay Virus Transmission and Infection in Caenorhabditis elegansbioRxiv - Microbiology 2024Quote: ... Orsay virus RNA1 level was quantified by qPCR using 1 μl of 1/5 diluted cDNA (GoTaq Promega A6001) and run on QuantStudio 3 Real Time PCR system (RNA1 qPCR primers GW194 and GW195)23.
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bioRxiv - Microbiology 2020Quote: ... Both NAD+ and NADH were quantified using NAD/NADH™-GLO kit (Promega) according to manufacturer’s instructions ...
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bioRxiv - Plant Biology 2019Quote: ... Genomic DNA was extracted from leaves using Wizard® Genomic Purification kit (Promega). Plant Tissue Protocol (Manufacturer protocol 3.E. ...
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bioRxiv - Cancer Biology 2021Quote: ... Glucose quantification was conducted using glucose bioluminescent assay kit (Glucose Glo Assay, Promega) as described per manufacturer instruction ...
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bioRxiv - Cancer Biology 2021Quote: ... and real-time PCR using GoTaq 2-Step RT-qPCR kit (Promega, A6110). All measurements were normalized against Actin as the internal control using the 2-ΔΔCt method (Figure 1—source data 3 and Figure 3—source data 2) ...
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bioRxiv - Cancer Biology 2021Quote: ... and DNaseI treated by Maxwell 16 LEV simplyRNA Cells Kit (Promega, Cat# AS1270) and Maxwell nucleic-acid extraction instrument (Promega) ...
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bioRxiv - Microbiology 2020Quote: ... Total GSH was measured from the supernatant using the GSH-Glo kit (Promega) following manufacturer’s instructions ...
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bioRxiv - Cell Biology 2020Quote: ... Relative NADPH levels were then determined using the NADPH-Glo assay kit (Promega) as per manufacturer’s instructions.
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bioRxiv - Cell Biology 2020Quote: ... and gene-body labeling was performed using the Prime-A-Gene kit (Promega).
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bioRxiv - Cell Biology 2020Quote: Endogenous ROS levels were measured using a ROS-GLO kit (Promega, Wisconsin, USA) according to manufacturer’s protocol ...
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bioRxiv - Immunology 2021Quote: ... while glutamate levels were measured using Glutamine/Glutamate-Glo™ Assay kit (Promega). αKG was measured using a colorimetric assay kit (Sigma Aldrich) ...
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bioRxiv - Neuroscience 2021Quote: ... OLIG2 immunostaining were applied after DeadEnd™ Fluorometric TUNEL System kit (Promega, G2350) to reveal TUNEL positive cells in OL lineage.
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bioRxiv - Developmental Biology 2021Quote: ... luciferase activity was measured with a dual-luciferase reporter assay kit (Promega; E1910), and the relative luciferase activity was determined by normalizing the firefly luciferase activity to the respective Renilla luciferase activity.
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bioRxiv - Developmental Biology 2021Quote: ... dual-luciferase activities were measured using the Dual-Glo Luciferase Assay Kit (Promega). A pRL-TK vector containing the Renilla luciferase gene was used as an internal control.
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bioRxiv - Microbiology 2022Quote: ... FLuc and RLuc activity were quantified using the Dual Luciferase Assay Kit (Promega) and read on a GloMax multi-detection system (Promega).
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bioRxiv - Molecular Biology 2021Quote: ... The PCR products were cleaned up using PCR clean up kit (# A9282, Promega). sgRNAs were synthesized using the MEGAShortScript™ Kit (# AM1354 ...
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bioRxiv - Molecular Biology 2020Quote: Total RNA extraction was performed using Maxwell RSC simply RNA cell kit (Promega) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2021Quote: ... and we performed the luciferase assays using a dual-luciferase assay kit (Promega) and quantified them with Monolight 3010 (Becton Dickinson).
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bioRxiv - Neuroscience 2021Quote: ... Luciferase activity was measured by lytic assay using NanoLuc (Promega Nano-Glo kit). Viafluor 405 was used to normalize for number of cells per well ...
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bioRxiv - Molecular Biology 2021Quote: ... cells were lysed and luciferase activity assayed using a luciferase assay kit (Promega) as described in the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2021Quote: ... and purified twice with a miniprep kit (PureYield™ Plasmid Miniprep System, Promega). Plasmids were eluted with RNase free water and used as templates for in vitro gene expression (E ...
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bioRxiv - Developmental Biology 2022Quote: ... followed by reverse transcription to generate cDNA with GoScript Reverse Transcription Kit (Promega). Quantitative PCR was performed by using the Agilent Mx3000P qPCR system with the 2×SYBR Green qPCR Master Mix (Bimake) ...
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bioRxiv - Immunology 2021Quote: ... Cartridges were further washed with Rapid digestion buffer (Promega, Rapid digestion buffer kit) and bound proteins were subjected to on-column digestion using mass spec grade Trypsin/Lys-C Rapid digestion enzyme (Promega ...