Labshake search
Citations for Promega :
1901 - 1950 of 5313 citations for 6 Bromo 3 3H imidazol 4 ylmethylene 1 3 dihydro indol 2 one since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2021Quote: ... the activities of the Firefly and NanoLuc luciferases were measured in a TriStar2 LB942 luminometer (Berthold) using the ONE-Glo Luciferase Assay System (Promega) and the Nano-Glo Live Cell Assay System (Promega).
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bioRxiv - Microbiology 2020Quote: ... MTS assays were performed to measure the cell viability using CellTiter 96 AQueous One reagent (G3580, Promega, Madison, WI, USA) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2019Quote: ... The CellTiter-Glo® Assay System and the ONE-Glo™ Luciferase Assay System were obtained from Promega (Madison WI).
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bioRxiv - Cancer Biology 2020Quote: Short term cell viability was assessed using the Cell Titer 96 AQeous One Solution MTS cell proliferation assay (Promega, UK), as previously described {Beeby ...
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bioRxiv - Cancer Biology 2022Quote: Relative cell viability was measured by MTS assays using the CellTiter 96® AQueous One Solution Cell Proliferation Assay (Promega). Briefly ...
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bioRxiv - Microbiology 2021Quote: ... Luciferase activity was measured 16 h post-infection using the Bright-Glo Luciferase Assay System or ONE-Glo Luciferase Assay System (Promega) and Centro xS960 luminometer (Berthold).
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bioRxiv - Microbiology 2021Quote: ... The virus-serum mix was subsequently used to infect A549-hACE2-TMPRSS2 cells7 for 18 hours at 37 °C before adding ONE-Glo reagent (Promega) for measurement of the luciferase signal by relative luminescence units (RLUs) ...
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bioRxiv - Molecular Biology 2019Quote: Cell viability was determined 72 h after PDS or cisplatin treatment using a CellTiter 96 AQueous One Solution Cell Proliferation Assay (Promega), according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2019Quote: ... Relative numbers of viable cells in each well were determined using CellTiter 96 AQueous One Solution Cell Proliferation Assay (Promega). Experiments were performed in six biological replicates ...
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bioRxiv - Microbiology 2021Quote: ... The virus-serum mix was subsequently used to infect A549-hACE2-TMPRSS2 cells [41] for 18 hours at 37°C before adding ONE-Glo reagent (Promega) for measurement of the luciferase signal by relative luminescence units (RLUs) ...
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bioRxiv - Microbiology 2019Quote: ... Parasites viability was assessed by direct light microscope observations and MTS colorimetric assays (CellTiter Aqueous One Solution Cell Proliferation Assay -Promega); they maintained shape and motility at 5 ...
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bioRxiv - Microbiology 2020Quote: ... Cell viability was evaluated using the MTS-based CellTiter 96® AQueous One Solution Cell Proliferation Assay kit (Promega, G3582), which determines viable cell number measuring the conversion at 490 nm of 3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium (MTS ...
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bioRxiv - Microbiology 2021Quote: ... culture supernatant was removed from each well and replaced with 0.3 ml of ONE-Glo luciferase reagent (Promega, Madison, WI). The plates were shaken at 400 rpm for 10 min at room temperature ...
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bioRxiv - Immunology 2021Quote: Toxicity was measured by quantifying the cell viability using the CytoTox-ONE™ Homogeneous Membrane Integrity Assay kit (Promega, G7891) according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2021Quote: ... cellular cytotoxicity was evaluated using the CellTiter 96® AQueous One Solution Cell Proliferation Assay kit using manufacturer’s instructions from Promega. Briefly ...
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bioRxiv - Microbiology 2020Quote: ... the purified products were quantified with the QuantiFluor® ONE dsDNA System by a QuantusTM Fluorometer E6150 (Promega, Madison, USA).
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bioRxiv - Immunology 2021Quote: ... cells were transfected with one of the Gag-mCherry variants (8 µg) using FuGene HD according to the manufacturer’s instructions (Promega, USA) in a 3:1 ratio with DNA ...
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bioRxiv - Immunology 2022Quote: ... Cell viability was assessed using a Cell Titer 96 AQueous One Solution Cell Proliferation Assay (Promega Corp, Madison, Wisconsin, USA). Absorbance measurements at 490 nm were normalized to the maximum read per cell line ...
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bioRxiv - Microbiology 2022Quote: Cell viability of different 2D cultures at different time points of infection or exposure to virus proteins or HAE supernatant was determined using the CellTiter 96 AQueous One Solution cell proliferation assay (G3582; Promega), while HTO viability was determined by the CellTiter-Glo 3D cell viability assay (Promega G9681 ...
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bioRxiv - Molecular Biology 2023Quote: ... The indicated DNA oligonucleotide and oligonucleotide ‘HDVrt’ were mutually extended upon one another using three thermocycles with GoTaq Hot-start DNA polymerase (Promega). This generated a double-stranded DNA comprising the DNA sequence of the desired RNA oligonucleotide ...
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bioRxiv - Microbiology 2022Quote: Cell viability was measured using a commercially available kit [CellTiter 96 Aqueous One Solution Cell Proliferation Assay (Promega, Madison, USA)] ...
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bioRxiv - Zoology 2022Quote: ... The sperm suspension was then divided into two samples: one for determining ATP using a CellTiter-Glo Luminescent Cell Viability Assay kit (Promega) and the other for assessing DNA content (to calibrate the ATP content by the number of sperm cells ...
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bioRxiv - Synthetic Biology 2024Quote: ... Four microliters of CFPS sample were added to 30 μL of ONE-Glo Luciferase Assay System (Promega, Madison WI, USA) in a white 96-well plate (Costar 3693 ...
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bioRxiv - Plant Biology 2024Quote: ... total RNA was extracted from one week-old Arabidopsis seedlings and reverse transcribed into cDNA using M-MLV reverse transcriptase (Promega) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2024Quote: ... The quality of the DNA was estimated using Nanodrop and the concentration was measured using the QuantiFluor ONE dsDNA System (Promega).
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bioRxiv - Evolutionary Biology 2024Quote: ... Genomic DNA was isolated and purified using the Wizard Genomic DNA Purification Kit protocol for Gram negative bacteria at one-third volume (Promega), then quantified using SYBR Green I Nucleic Acid Stain (Invitrogen ...
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Phospholipase C β4 promotes RANKL-dependent osteoclastogenesis by interacting with MKK3 and p38 MAPKbioRxiv - Cell Biology 2024Quote: ... the existing media was removed and replaced with 100 µL of medium containing 20 µL of CellTiter 96 AQueous One solution (Promega). The cells were then incubated for 4 h at 37°C in a humified atmosphere with 5% CO2 ...
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bioRxiv - Genomics 2024Quote: ... The final DNA yield (17.3 µg) was quantified using a Quantus Fluorometer (QuantiFluor ONE dsDNA Dye assay; Promega, Madison, WI). The size distribution of the HMW DNA was estimated using the Femto Pulse system (Agilent ...
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bioRxiv - Bioengineering 2024Quote: ... Cell viability and in vitro luciferase expression was evaluated via ONE-Glo + Tox luciferase reporter and cell viability assay kit (Promega) and a multimode microplate reader.
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bioRxiv - Microbiology 2023Quote: ... were infected with VSV-G-pseudotyped NL4-3Δenv-luc at a MOI of 0.0001 and Luciferase expression was quantified after two days using the One-Glo™ luciferase assay (Promega) as described previously [8].
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bioRxiv - Microbiology 2022Quote: ... allowing to adhere for 24 h before addition of a serial dilution of inhibitor and measurement of cell viability 72 h later using the CellTiter AQueous One solution (Promega), following manufacturer’s instructions ...
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bioRxiv - Immunology 2022Quote: ... Cell viability was assessed using a Cell Titer 96 AQueous One Solution Cell Proliferation Assay (Promega Corp, Madison, Wisconsin, USA). Absorbance measurements at 490 nm were normalized to the maximum read per cell line ...
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bioRxiv - Molecular Biology 2022Quote: ... Next two overlapping fragments containing one of the homologies and a part of the antibiotic marker were generated by PCR using Taq polymerase (Promega), which overlap for a length of 594 bp ...
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bioRxiv - Microbiology 2022Quote: ... allowing to adhere for 24 h before addition of a serial dilution of protease inhibitors and measurement of cell viability 72 h later using the CellTiter AQueous One solution (Promega), following manufacturer’s instructions.
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bioRxiv - Molecular Biology 2023Quote: ... Equal amounts of the lysate were transferred into two Microtiter plates (one for Renilla and the second for Bright Glo (Promega)) ...
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bioRxiv - Neuroscience 2023Quote: LDH release from macrophages treated with Aβ profect complexes was measured using the CytoTox-ONE Homogeneous Membrane Integrity Assay (cat no: G7891, Promega) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... Cell proliferation was assessed every 24 h by the MTS assay (CellTiter 96® AQueous One Solution Cell Proliferation, Promega). DMSO-treated cells were used as negative controls and the effects on cell proliferation were expressed as the percentage inhibition compared to the cells of the control group ...
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bioRxiv - Systems Biology 2023Quote: Cell viability was measured using a commercial kit (Cell Titre 96 AQueous One Solution cell proliferation assay; Promega, Madison, USA) that uses a tetrazolium salt-based colorimetric assay [20] ...
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bioRxiv - Developmental Biology 2023Quote: ... After 7 days of culture the MTS cell viability reagent (CellTiter 96® Aqueous One Solution Cell Proliferation Assay, Promega) was added and plates incubated for 4 hours at 37°C ...
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bioRxiv - Cancer Biology 2023Quote: Cell proliferation colorimetric-based assays were performed using 4,000 transduced or transfected cells and the CellTiter 96 Aqueous One Solution Cell Proliferation Assay (Promega, G3582) according to the manufacturer’s instructions for 96h ...
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bioRxiv - Synthetic Biology 2024Quote: ... 0.06pmol of donor backbone and 0.04pmol of All-in-One backbone were co-transfected in HEK293T using FuGENEHD (FuGENE® HD Transfection Reagent, Promega) as transfection reagent at a ratio of 3:1 FuGENEHD (μL) ...
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bioRxiv - Microbiology 2024Quote: ... Cell viability was measured using a commercially available kit [CellTiter 96 Aqueous One Solution Cell Proliferation Assay (Promega, Madison, USA)] ...
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bioRxiv - Evolutionary Biology 2024Quote: ... The total RNA of each species was extracted from male and female nymphs (each one individual, whole body) using ISOGEN □ (Nippon Gene) and ReliaPrep RNA Tissue Miniprep Systems (Promega). Total RNA extracted from a male and female nymph was mixed in equal amounts ...
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bioRxiv - Microbiology 2021Quote: ... 2 μg RNA was incubated at 30 °C for 2 hr in Spodoptera frugiperda (Sf21) extract (Promega) in the presence of [35S]methionine-cysteine (Perkin-Elmer ...
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bioRxiv - Molecular Biology 2023Quote: ... cells were transfected with 20 ng/well of psiCHECK-2 plasmid (psiCHECK-2 Vector (V0) (Promega, C8021) or let-7a-mi6 targeting six regions of the 3ill UTR ...
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bioRxiv - Immunology 2021Quote: ... in a mixture with 20 μl OptiMEM and 1μl FuGENE HD or FuGENE 6 (Promega E2691) per well ...
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bioRxiv - Molecular Biology 2020Quote: ... Proteins were digested with Lys-C (Wako Chemicals) for 6 hours and trypsin (Trypsin Gold, Promega) overnight.
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bioRxiv - Cell Biology 2022Quote: ... HeLa cells were cultured in DMEM+10% FBS+Penicillin/Streptomycin and transfected using FuGENE 6 (Promega). Co-recruitment assays were performed 24 hours after transfection ...
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bioRxiv - Immunology 2020Quote: All VLPs were produced by transient transfection of HEK293T cells with Fugene 6 (Promega, ref E2691). HEK293T were seeded in 15-cm dishes to reach 60-70% confluency the next day and VLPs were produced by co-transfecting plasmids encoding Gag-eGFP and the VSV-G envelope (pGag-EGFP and pCMV-VSV-G ...
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Safety and efficacy of C9ORF72-repeat RNA nuclear export inhibition in amyotrophic lateral sclerosisbioRxiv - Systems Biology 2021Quote: Three wells of a 6-well plate were lysed were lysed in Reporter lysis buffer (Promega) for 10 min on ice before centrifugation at 17,000 g ...