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Citations for Promega :
1851 - 1900 of 2772 citations for Tubulin Delta 1 TUBD1 Antibody HRP since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2023Quote: ... qPCR reactions were performed using the GoTaq 1-Step RT-qPCR System (Promega GmbH, Walldorf, Germany). 100 ng of isolated mRNA were used per reaction ...
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bioRxiv - Biochemistry 2023Quote: ... 10 µg of reconstituted protein was digested in 0.05 N HCl with 1 µg pepsin (Promega) without prior reduction/alkylation at a volume of 200 µl 50 °C for 3 h ...
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bioRxiv - Molecular Biology 2023Quote: ... Cells were resuspended in 1 mL of the same buffer containing protease inhibitor cocktail (Promega, USA), and then transferred to 2 mL lysing matrix B tubes containing 0.1 mm diameter silica beads (MP Biomedicals ...
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bioRxiv - Bioengineering 2024Quote: ... RT-qPCR was performed using the GoTaq Probe 1-Step RT-qPCR System (Promega Cat# A6120). RT-qPCR primers and probes were listed in Supplementary Data 2.
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bioRxiv - Microbiology 2023Quote: ... Complimentary DNA was prepared from 1 µg of RNA using a Reverse Transcription Systems Kit (Promega). Quantitative PCR (qPCR ...
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bioRxiv - Microbiology 2024Quote: ... Viral RNA was quantified by real-time RT-qPCR (GoTaq 1 step RT-qPCR kit, Promega). Quantification was provided by serial dilutions of an appropriate T7-generated synthetic RNA standard ...
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bioRxiv - Molecular Biology 2024Quote: ... cDNA was synthesized with 1 μg/μL of RNA concentration using MMLV-RT enzyme (Promega, USA). HvHsfA6a gene specific and Actin primers designed by using Primer express 3.0 software (Thermoscientific USA ...
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bioRxiv - Cell Biology 2024Quote: ... Nano-Glo assay reagent consisted of a 1:1000 dilution of Nano-Glo substrate (Promega; N1110) in buffer (100mM 2-(N-Morpholino)ethanesulfonic acid hydrate (M8250 ...
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bioRxiv - Developmental Biology 2024Quote: ... They were then embedded on the side position in 1% low melting agarose (Promega, Cat# V2111) in a glass bottom 60μ-Dish (35mm high ...
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bioRxiv - Cell Biology 2024Quote: ... cells were transfected with TFG-mEGFP-FLAG at 1 µg/µL using Fugene (Promega, cat # PRE2311) and left to express for 24 hours ...
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bioRxiv - Cancer Biology 2024Quote: 1 microgram total RNA extracted using ReliaPrep™ RNA Cell Miniprep System (Promega, Madison, WI, USA), were checked using the Agilent-2100 Bioanalyzer System and subjected to library preparation utilizing the TruSeq stranded total RNA Gold library preparation kit with RiboZero Gold treatment and unique dual indexes according to the manufacturer’s instructions (Illumina Inc. ...
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bioRxiv - Molecular Biology 2023Quote: ... 1/20 of the cDNA was amplified by PCR using the GoTaq DNA polymerase mix (Promega) with specific antigenome primers (Appendix Table S1) ...
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bioRxiv - Cell Biology 2020Quote: ... Samples were diluted to 1 M urea with 50 mM ambic and digested with Trypsin (Promega #V5111) for 10 hours at 1:125 enzyme:protein at 37°C ...
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bioRxiv - Microbiology 2021Quote: ... ribosome positioning was determined by primer extension/reverse transcription using AMV reverse transcriptase (1 U/μl) (Promega) in the presence of 125 μM of each of dTTP ...
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bioRxiv - Microbiology 2019Quote: ... The pellet was dissolved in 1% (w/v) RapiGest (Waters) (10 µL) and trypsin (Sequencing grade, Promega) solution was added (90 µL ...
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bioRxiv - Molecular Biology 2021Quote: ... lysed in 1 × passive lysis buffer and analyzed using the Dual-Luciferase Reporter Assay System (Promega Corporation) and a SpectraMax L Microplate Reader (Molecular Devices ...
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bioRxiv - Microbiology 2019Quote: ... The reaction mixture (25 μL) used for cDNA synthesis contained 1⋅25 μM random hexamer primers (Promega), 250 μM of each deoxynucleoside triphosphate (dNTP) ...
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bioRxiv - Cancer Biology 2019Quote: ... Protein digestion on beads was carried out using a ratio 1:50 sequencing grade modified trypsin (Promega): protein extract in 100 mM ammonium bicarbonate at 37 °C overnight ...
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bioRxiv - Plant Biology 2019Quote: ... using 1 - 2 µg of total RNA and 250 ng of a mixture of random hexanucleotides (Promega) and incubated for 50 min at 50°C ...
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bioRxiv - Immunology 2019Quote: ... In vitro pmel-1 T cell-mediated cytotoxicity assay with CytoTox-ONE Homogeneous Membrane Integrity Assay (Promega). protocols.io dx.doi.org/10.17504/protocols.io.6j4hcqw
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bioRxiv - Developmental Biology 2021Quote: ... the reaction mixtures for in vitro transcription were treated with RQ-1 RNase-free DNase I (Promega). Synthesized mRNAs were treated with phenol-chloroform to remove protein components ...
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bioRxiv - Biophysics 2021Quote: ... the cells were transfected with 1 – 2 μg EphA2-mTurquoise plasmid DNA using FuGene HD (Promega, #E2311) according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2020Quote: ... cells were transfected with 500 ng of either HDM-SARS-Spike-delta21 or HDM_Spike_RBD_B7-1 plasmids and 1.5 ng of Transfection Carrier DNA (Promega, E4881) using BioT reagent (Bioland Sci ...
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bioRxiv - Microbiology 2020Quote: ... RNA (1 μg) was converted to cDNA using the GoScript™ Reverse Transcription System (Promega, Madison, WI). The cDNA was synthesised was used for qPCR using the iQTM SYBR® Green Supermix (Bio-Rad Laboratories ...
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bioRxiv - Microbiology 2021Quote: ... At 1 × s after injection of 20 µl of the substrate solution (Renilla Luciferase Assay System, Promega), relative light units (RLUs ...
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bioRxiv - Cell Biology 2020Quote: 1 µg of total RNAs were reverse transcribed with Moloney murine leukemia virus Reverse Transcriptase (M170B, Promega). 100 ng cDNA and FastStart Universal SYBR Green Master (04913850001 ...
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bioRxiv - Cancer Biology 2022Quote: ... Proteins were digested in-gel using trypsin (Gold Promega, 1 μg per band, overnight at 30°C,) as previously described (Thouvenot et al. ...
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bioRxiv - Microbiology 2022Quote: ... Larvae were immobilized in the dish using a drop of 1% low-melting-point agarose (V2111, Promega) solution in 80% glycerol ...
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bioRxiv - Neuroscience 2022Quote: ... The samples were then incubated with 1:50 enzyme to protein ratio of sequencing-grade trypsin (Promega) overnight at 37 °C ...
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bioRxiv - Biophysics 2022Quote: ... The embryos were then incubated in a 1:5,000 dilution of TMR HaloTag Ligand (5 mM; Promega) in Danieau’s medium ...
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bioRxiv - Biochemistry 2022Quote: ... the samples were incubated with trypsin (trypsin to substrate ratio of 1:50, Promega, cat. no. V5111) and 1 mM CaCl2 for 16 h at 37°C ...
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bioRxiv - Plant Biology 2021Quote: ... For multiple day measurements plants were sprayed daily with 1 mM D-luciferin (Promega, Fitchburg, Wl, USA) at 10 am ...
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bioRxiv - Cancer Biology 2020Quote: ... Cells were incubated with cell-permeant TMR red Halo-tag ligand (1:2,000 for 15 min, Promega) 24 hours post-transfection ...
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bioRxiv - Microbiology 2020Quote: ... and 1 μg of a plasmid expressing the VSV G glycoprotein using the FuGene HD reagent (Promega). Forty-eight hours later ...
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bioRxiv - Neuroscience 2022Quote: ... The samples were then incubated with 1:50 enzyme to protein ratio of sequencing-grade trypsin (Promega) overnight at 37 °C ...
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bioRxiv - Evolutionary Biology 2022Quote: ... Bacterial DNA was extracted from 1 ml of culture using the Wizard genomic DNA purification kit (Promega). Evolved population DNAs were sequenced at the GeT-PlaGe core facility (https://get.genotoul.fr/) ...
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bioRxiv - Immunology 2019Quote: ... at 37ºC for 1 hr followed by treatment with 20 µg proteinase K (Promega, Madison, WI, USA) at 45ºC for 1 hr to remove protein ...
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bioRxiv - Developmental Biology 2019Quote: ... according to manufacturer’s instructions with the addition of 1 µl RNAse inhibitor (RNAsin Plus RNase Inhibitor, Promega).
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bioRxiv - Systems Biology 2019Quote: ... Proteins were digested on-filter (16 hr, 37 °C) with sequencing-grade modified trypsin (50:1, Promega). Proteolysis was terminated and peptides eluted with 20 μL of 10% trifluoroacetic acid followed by centrifugation (13,000 ×g ...
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bioRxiv - Cancer Biology 2020Quote: ... cells were resuspended in 400μL FACS buffer (1.5% BSA, 2.5mM HEPES, 1mM EDTA in PBS) containing 2.5% RNAsin and 1/10000 DAPI (Promega). Cells for re-injection experiments were isolated as above and subjected to a further two centrifugation steps (1min at 200xg ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... at a ratio of 1:166 to Opti-MEM media in combination with extracellular NanoLuc Inhibitor (Promega) diluted 1:500 (10 μL [30 mM stock] per 5 mL Opti-MEM plus substrate) ...
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bioRxiv - Genomics 2021Quote: ... cells were transfected in a 3:1 ratio of FuGENE® HD Transfection Reagent (Promega cat # E2311) and plasmid DNA (pcDNA3.1-HMGB1P1-3xHA ...
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bioRxiv - Physiology 2020Quote: ... proteins were digested by incubation with sequencing-grade modified trypsin (1/50, w/w; Promega, Madison, Wisconsin) for 12 h at 37°C ...
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bioRxiv - Cell Biology 2020Quote: ... Nluc and BRET-FL signals were sequentially acquired by IV-injection with 1/40 diluted furimazine (Promega) in 100 μL sterile PBS and applying open (for Nluc ...
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bioRxiv - Microbiology 2021Quote: ... proteins were digested overnight with sequencing-grade porcine trypsin (1:25, w/w, Promega, Fitchburg, MA, USA). The resulting vacuum-dried peptides were resuspended in water containing 0.1% (v/v ...
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bioRxiv - Immunology 2019Quote: ... were transfected with indicated 1.33 μg HIV-1 env DNA and 2.66 μg Q23gΔenv using Fugene6 (Promega), harvested 48 hr post-transfection ...
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bioRxiv - Molecular Biology 2019Quote: ... cDNA synthesis was performed on 1 µg of RNA using ProtoScript First Strand cDNA Synthesis Kit (Promega). Primers recognizing exons flanking the CRISPR-targeted exon (Supp ...
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bioRxiv - Genetics 2019Quote: ... proteins were digested overnight with sequencing-grade porcine trypsin (1:25, w/w, Promega, Fitchburg, MA, USA). The resulting vacuum-dried peptides were resuspended in water containing 0.1% (v/v ...
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bioRxiv - Microbiology 2019Quote: ... 1 μg of total RNA template was reverse transcribed to cDNA by M-MLV reverse transcriptase (Promega) using specific stem-loop RT primer and under the following conditions ...
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bioRxiv - Genomics 2021Quote: ... Diluted cDNA (1:20) was used in a PCR reaction with the GoTaq qPCR Master Mix (Promega) and run on a CFX384 Touch instrument (Bio-Rad).