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Citations for Promega :
1801 - 1850 of 5936 citations for Mouse Lymphoid Enhancer Binding Factor 1 LEF1 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Physiology 2024Quote: ... (Promega #E1941, diluted 1:5 with water). Dual-Glo Luciferase assays were performed to measure Firefly and Renilla luciferase activity (Promega #E2940) ...
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bioRxiv - Developmental Biology 2023Quote: ... and 0.5 U μl-1 RNAsin (Promega)] ...
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bioRxiv - Physiology 2024Quote: ... with 1 µg Lys-C/Trypsin (Promega) and incubated at 37°C overnight.
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bioRxiv - Cancer Biology 2024Quote: ... 1 μl GoTaq Hot Start (Promega M5005) and 200 ng UMI-4C DNA template ...
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bioRxiv - Molecular Biology 2022Quote: ... Standard curves for each primer set were generated using serially diluted plasmid (for gAAV) or Human and Mouse Genomic DNA (for host) (Promega G1521 and G3091, respectively) and used for quantification ...
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bioRxiv - Cancer Biology 2021Quote: ... the samples were diluted to 1 M Urea with 10mM EPPS pH 8.2 and incubated overnight with 1:50 LysC (Wako Chemicals) and 1:100 Sequencing grade trypsin (Promega). Digests were acidified using TFA and tryptic peptides were purified by tC18 SepPak (50 mg ...
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bioRxiv - Microbiology 2022Quote: ... cDNA was diluted either 1:5 or 1:10 for all qPCR experiments and GoTaq qPCR Master Mix (Promega) was used to amplify cDNA ...
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bioRxiv - Cell Biology 2022Quote: ... Beads were washed three times for 10 min with gentle rotation in high-salt buffer (50 mM Tris, pH 7.5, 300 mM KCl, 12 mM MgCl2, 1% Nonidet P-40, 1 mM DTT, 100U/mL Promega RNasin ...
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bioRxiv - Cell Biology 2022Quote: ... polysome were stabilised by washing cells in 20 ml ice-cold water containing 0.1 mg/ml CHX before being resuspended in 1 ml RiboTag Lysis Buffer (50 mM Tris, pH 7.5, 100 mM KCl, 12 mM MgCl2, 1% Nonidet P-40, 1 mM DTT, 100U/mL Promega RNasin ...
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bioRxiv - Biophysics 2020Quote: ... All samples were brought to room temperature and mixed with a 1:1 volume of nGlow solution (Promega, #N1110). Samples (90% of total volume ...
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bioRxiv - Cancer Biology 2021Quote: ... the samples were diluted to 1 M Urea with 10 mM EPPS pH 8.2 and incubated overnight with 1:50 LysC (Wako Chemicals) and 1:100 Sequencing grade trypsin (Promega). Digests were acidified using TFA and tryptic peptides were purified by Oasis Prime HLB columns (30 mg ...
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bioRxiv - Biophysics 2021Quote: ... All samples were brought to room temperature and mixed with a 1:1 volume of nGlow solution (Promega, #N1110). Samples (90% of total volume ...
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bioRxiv - Developmental Biology 2022Quote: HEK293T cells were simultaneously transfected with 1 μg pCS2Cachd1-eGFP or pCS2Cachd1_V1122D-eGFP and 1 μg KDEL-tRFP plasmid using Fugene 6 transfection reagent (Promega). eGFP/tRFP expression was confirmed 24 hours post transfection and the cells fixed at 42 hours post transfection and imaged on a spinning disk microscope (see below).
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bioRxiv - Molecular Biology 2019Quote: ... Cell pellets were lysed in 300µl of Lysis Buffer (50mM Tris-HCl pH 7.5, 150mM NaCl, 1% Triton® X-100, 0.1% deoxycholate, Protease Inhibitor and RNasin Plus [1:500, Promega]),diluted to a final concentration of ~1µg/µl with 100mM Tris-HCl pH 7.5 ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... Cell media was then replaced with a 1:1 mixture of fresh media and Nano-Glo Luciferase reagent (Promega) and luminescence was measured using a SpectroMax M5e (Molecular Devices) ...
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bioRxiv - Immunology 2021Quote: ... The media from infected cells was then removed and 100 μl of 1:1 diluted PBS:Bio-Glo (Promega, G7940) luciferase substrate was added to each well ...
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bioRxiv - Cancer Biology 2020Quote: ... the samples were diluted to 1 M Urea with 10mM EPPS pH 8.2 and incubated overnight with 1:50 LysC (Wako Chemicals) and 1:100 Sequencing grade trypsin (Promega). Digests were acidified using TFA and tryptic peptideswere purified by tC18 SepPak (50 mg ...
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bioRxiv - Microbiology 2021Quote: ... the cells were lysed with a 1:1 ratio of virus to Bright-Glo luciferase assay media (Promega, #E2610) and the contents were analyzed on a luminometer (LUMISTAR Omega ...
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bioRxiv - Microbiology 2022Quote: ... cells were lysed by adding 100 µl of 1:1 (v:v) mixture of phenol red-free DMEM and Bright-Glo substrate (Promega) and luminescence detected using a Glomax Navigator (Promega) ...
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bioRxiv - Molecular Biology 2022Quote: ... 1 mM DTT) in presence of 1 mM Adenylyl-imidodiphosphate (AMP-PNP) and RNase inhibitor RNAsin (0.5 U, Promega) for 25 min at 4°C with head-over-end rotation ...
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bioRxiv - Immunology 2022Quote: ... caspase-1 activity was quantified in supernatants using a commercially available assay (Caspase-GLO 1 Assay; Promega, Madison, WI) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2023Quote: ... Media were removed and 100 μl of CTG reagent (CellTiter-Glo®, Promega GmbH, Germany; 1:1 with PBS) per well was added ...
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bioRxiv - Microbiology 2023Quote: ... CM was then harvested by centrifugation and active caspase-1 measured with the Caspase-Glo 1 Inflammasome Assay (Promega) following the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2023Quote: ... Sample C was treated with 1% Triton X-100 (TX100) followed by treatment with 1μg mL-1 trypsin (Promega). For the detergent treatment ...
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bioRxiv - Bioengineering 2023Quote: ... The reaction was initiated by the addition of 1-250 nM acylated peptide (Vivitide) and a 1:10,000 dilution of LgBiT subunit of the NanoBiT luciferase (Promega) and read using an Envision plate reader (XCite 2105 ...
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bioRxiv - Genomics 2022Quote: ... Nuclei were resuspended in 200 μL resuspension buffer (1x PBS, 1% BSA and 0.2U μl/1 RNasin Plus RNase Inhibitor (Promega)) and transferred to a 1.5 mL Eppendorf tube tube ...
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bioRxiv - Neuroscience 2023Quote: ... and Dounce homogenized in 500 µL of ice-cold RNase-free buffer polysome buffer (50 mM Tris pH 7.5, 100 mM KCl, 12 mM MgCl2, 1% NP-40, 1 mM DTT, 200U/ml Promega RNasin ...
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bioRxiv - Developmental Biology 2023Quote: ... a minimum of 1 µg of RNA was combined with 1 µl of Oligo (dT)15 primer (Promega, C110B) and nuclease-free water to make up a total of 5 µl and incubated at 70°C and 4°C for 5 min each ...
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bioRxiv - Developmental Biology 2023Quote: ... A minimum of 1 µg of RNA was combined with 1 µl of Oligo (dT)15 primer (Promega, C110B) and nuclease-free water to make up a total of 5 µl and incubated at 70°C and 4°C for 5 min each ...
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bioRxiv - Molecular Biology 2023Quote: ... Two μL of reverse transcription buffer 5X were added and the samples were incubated at 20°C for 15 min before addition of 1.5 μL of dNTP mix (1 mM of dATP, dGTP, dCTP, dUTP) and 1 μL of AMV enzyme (PROMEGA). Reverse transcription was performed at 42°C for 45 min and synthesized cDNA was recovered by ethanol precipitation as described above ...
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bioRxiv - Biophysics 2023Quote: ... we stained the cells with 1 ml of a 1:2,000 TMR Halo-ligand solution (Promega, Cat. No.: G8251) to fluorescently label the Halo-tagged proteins ...
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bioRxiv - Molecular Biology 2024Quote: ... cells were incubated with the primary antibodies (1D4 1:1500, Anti-Calnexin Sigma c4731 1:600, Anti-LgBiT Promega N7100 1:100 ...
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Bacteria Are a Major Determinant of Orsay Virus Transmission and Infection in Caenorhabditis elegansbioRxiv - Microbiology 2024Quote: ... Orsay Virus RNA1 level was quantified by qPCR using 1 μl of 1/5 diluted cDNA (GoTaq Promega A6001) and run on QuantStudio 3 Real Time PCR system ...
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Bacteria Are a Major Determinant of Orsay Virus Transmission and Infection in Caenorhabditis elegansbioRxiv - Microbiology 2024Quote: ... Orsay virus RNA1 level was quantified by qPCR using 1 μl of 1/5 diluted cDNA (GoTaq Promega A6001) and run on QuantStudio 3 Real Time PCR system (RNA1 qPCR primers GW194 and GW195)23.
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bioRxiv - Microbiology 2020Quote: ... Both NAD+ and NADH were quantified using NAD/NADH™-GLO kit (Promega) according to manufacturer’s instructions ...
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bioRxiv - Plant Biology 2019Quote: ... Genomic DNA was extracted from leaves using Wizard® Genomic Purification kit (Promega). Plant Tissue Protocol (Manufacturer protocol 3.E. ...
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bioRxiv - Cancer Biology 2021Quote: ... Glucose quantification was conducted using glucose bioluminescent assay kit (Glucose Glo Assay, Promega) as described per manufacturer instruction ...
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bioRxiv - Cancer Biology 2021Quote: ... and real-time PCR using GoTaq 2-Step RT-qPCR kit (Promega, A6110). All measurements were normalized against Actin as the internal control using the 2-ΔΔCt method (Figure 1—source data 3 and Figure 3—source data 2) ...
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bioRxiv - Cancer Biology 2021Quote: ... and DNaseI treated by Maxwell 16 LEV simplyRNA Cells Kit (Promega, Cat# AS1270) and Maxwell nucleic-acid extraction instrument (Promega) ...
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bioRxiv - Microbiology 2020Quote: ... Total GSH was measured from the supernatant using the GSH-Glo kit (Promega) following manufacturer’s instructions ...
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bioRxiv - Cell Biology 2020Quote: ... Relative NADPH levels were then determined using the NADPH-Glo assay kit (Promega) as per manufacturer’s instructions.
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bioRxiv - Cell Biology 2020Quote: ... and gene-body labeling was performed using the Prime-A-Gene kit (Promega).
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bioRxiv - Cell Biology 2020Quote: Endogenous ROS levels were measured using a ROS-GLO kit (Promega, Wisconsin, USA) according to manufacturer’s protocol ...
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bioRxiv - Immunology 2021Quote: ... while glutamate levels were measured using Glutamine/Glutamate-Glo™ Assay kit (Promega). αKG was measured using a colorimetric assay kit (Sigma Aldrich) ...
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bioRxiv - Neuroscience 2021Quote: ... OLIG2 immunostaining were applied after DeadEnd™ Fluorometric TUNEL System kit (Promega, G2350) to reveal TUNEL positive cells in OL lineage.
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bioRxiv - Developmental Biology 2021Quote: ... luciferase activity was measured with a dual-luciferase reporter assay kit (Promega; E1910), and the relative luciferase activity was determined by normalizing the firefly luciferase activity to the respective Renilla luciferase activity.
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bioRxiv - Developmental Biology 2021Quote: ... dual-luciferase activities were measured using the Dual-Glo Luciferase Assay Kit (Promega). A pRL-TK vector containing the Renilla luciferase gene was used as an internal control.
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bioRxiv - Microbiology 2022Quote: ... FLuc and RLuc activity were quantified using the Dual Luciferase Assay Kit (Promega) and read on a GloMax multi-detection system (Promega).
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bioRxiv - Molecular Biology 2021Quote: ... The PCR products were cleaned up using PCR clean up kit (# A9282, Promega). sgRNAs were synthesized using the MEGAShortScript™ Kit (# AM1354 ...
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bioRxiv - Molecular Biology 2020Quote: Total RNA extraction was performed using Maxwell RSC simply RNA cell kit (Promega) according to the manufacturer’s instructions ...