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Citations for Promega :
1801 - 1850 of 3123 citations for 6 Phenyl hexa 3 5 dien 2 one since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: ... HEK293T cells were transfected with packaging vectors pMD2.G and psPAX2 along with relevant shRNA using Fugene 6 transfection reagent (Promega). Viral particles were harvested after 48 hours in DEMEM/ 40% FBS ...
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bioRxiv - Microbiology 2023Quote: ... 8 × 105 HEK293T cells/well were seeded into 6-well plates and transfected the next day with ViaFect (Promega, E4981), using either 4 μg of plasmid expressing the G protein or 2 μg of plasmid expressing HA together with 2 μg of plasmid expressing NA ...
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bioRxiv - Genetics 2023Quote: ... Protein mixtures were diluted in 1:6 ratio (v/v) using ultrapure water prior to digestion using sequencing grade trypsin (Promega) at 37 °C for 16 h ...
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bioRxiv - Biochemistry 2023Quote: ... by transfection of the pSpCas9(BB)-2A-Puro (PX459) plasmid containing A3B gRNAs with FuGENE 6 Transfection Reagent (E2691; Promega). 16h after transfection ...
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bioRxiv - Cancer Biology 2024Quote: ... Cell viability was assayed starting at Day 1 for 6 days according to the CellTiter-Glo 3D kit (Promega G9683). For the antiandrogen response assay ...
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bioRxiv - Cell Biology 2023Quote: ... mammalian expression vectors containing the fusion gene under the control of a weak L30 promoter and containing a Neomycin resistance marker were transfected into U2OS cells at 70% confluence using FuGENE 6 (Cat. No. E2691, Promega). Transfected cells were selected with G418 (Cat ...
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bioRxiv - Microbiology 2023Quote: ... Guides targeting 5’ and 3’ regions (see primer sequences in Supplementary File 6) of the PIP5Pase coding sequence were produced by in vitro transcription with T7 polymerase (Promega), and co-transfected with recombinant saCas9 Nuclease NLS Protein (Applied Biological Materials Inc.) ...
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bioRxiv - Cell Biology 2023Quote: ... cells grown to ∼70% confluence in 6-well plates and co-transfected with a gRNA to TBC1D23 (12) and pmaxGFP using Fugene6 (Promega) according to manufacturer’s instruction ...
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Arc mediates intercellular synaptic plasticity via IRSp53-dependent extracellular vesicle biogenesisbioRxiv - Neuroscience 2024Quote: ... plated in 6-well dishes were centrifuged at 1000xg for 10 minutes and suspended in 5x Passive Lysis buffer (Promega). Each data point represents an individual well ...
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bioRxiv - Biochemistry 2024Quote: ... at a 1:75 enzyme to protein ratio for 6 h at 30 °C and then by trypsin (V5111, Promega) (1:50 enzyme to protein ratio ...
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bioRxiv - Cell Biology 2024Quote: ... and RSPO1-CM were produced as described (Fenderico et al., 2019; Tauriello et al., 2010) Transfections were performed using either FuGENE 6 (Promega) according to manufacturer’s protocol for β-catenin-mediated reporter assays and microscopy or polyethylenimine (PEI ...
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bioRxiv - Neuroscience 2023Quote: Approximately 5x104 HEK293 cells were seeded on poly-L-lysine coated glass coverslips and transfected with expression constructs for SORL1-WT or SORL1-R953C using Fugene 6 Transfection Reagent kit (Promega). 24h post-transfection ...
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bioRxiv - Biochemistry 2019Quote: ... The samples were then diluted with 150 μL PBS and to this was added 1 mM CaCl2 (2 μL of a 200 mM stock in water) and trypsin (2 μg, Promega, sequencing grade, V5111) and the digestion was allowed to digest overnight at 37 °C with shaking ...
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bioRxiv - Cancer Biology 2021Quote: The 3’UTR of human PAQR3 was cloned into pmirGLO vector (Promega, Madison, WI, USA), and then validated by sequencing ...
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bioRxiv - Cell Biology 2020Quote: ... Then samples were diluted 3 times in the buffer and 1 µg trypsin (Gold. Promega) was added overnight at 30°C ...
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bioRxiv - Molecular Biology 2020Quote: ... Caspase activity was determined using Caspase-Glo® 3/7 Assay Systems (Promega, Madison, WI) according to the manufacturer’s specifications ...
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bioRxiv - Cell Biology 2019Quote: ... Survival after 3 days of treatment was measured by the use of Resazurin (Promega #G8081). Cells were incubated with Resazurin for 3 hours ...
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bioRxiv - Developmental Biology 2019Quote: ... The Promega Caspase-Glo® 3/7 Assay Systems (Promega, Madison, WI, Cat No. G8091) was used to quantify apoptotic cell death
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bioRxiv - Microbiology 2021Quote: ... caspase activity was measured using Caspase-Glo 3/7 Assay according to manufacturer’s instructions (Promega). Staurosporine (1μM ...
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bioRxiv - Biochemistry 2020Quote: FLAG-β2AR HEK 293 cells were transfected with 3 µg cAMP luciferase plasmid DNA (Promega) using Turbofect (ThermoFisher ...
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bioRxiv - Neuroscience 2020Quote: Caspase activity was measured using a chemiluminescent assay kit (Promega Caspase-Glo® 3/7) in 96-well format using an Analyst HT plate reader (Molecular Devices ...
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bioRxiv - Cancer Biology 2020Quote: ... Plasmid transfections required 3 μg/well and were carried out using 0.1% Fugene HD (Promega).
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bioRxiv - Cell Biology 2021Quote: ... and incubated for 24 hr before adding 40 μl of Caspase-Glo 3/7 (Promega) to each well ...
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bioRxiv - Cancer Biology 2021Quote: ... The remaining cells in the inserts were analyzed with Caspase-Glo 3/7 assay (Promega) to confirm that Jurkat cells incubated with TAM CM did not induce apoptosis compared to the control medium.
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bioRxiv - Biophysics 2019Quote: ... A total of 3 μg plasmid and 9 μL of FuGENE HD Transfection Reagent (Promega) were delivered into each well following manufacturer’s instruction ...
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bioRxiv - Neuroscience 2021Quote: ... HA-tagged NPHP1 expression vectors were transfected to mIMCD-3 cells using FuGENE HD (Promega) following the manufacturer’s instruction ...
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bioRxiv - Cell Biology 2020Quote: ... Caspase activation was measured using the CaspaseGlo 3/7 Assay Kit (Promega, Madison, WI, USA) according to manufacturer’s instructions for the indicated time point presented in the figure legend ...
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bioRxiv - Genomics 2019Quote: ... Quantitative PCR was performed in 3 technical replicates with the GoTaq qPCR Master Mix (Promega) in 384 well plates ...
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bioRxiv - Microbiology 2020Quote: ... PacC222 and PacC80 proteins were individually expressed in the pGEX-4T-3 vector (Promega, USA) in E ...
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bioRxiv - Genomics 2021Quote: All the reporter constructs were cloned into pGL-3 promoter plasmids (Promega, Madison, WI, USA). Fragments of the candidate RSCNEs were cloned into pGL3-promoter vector digested by BamH I and Sal □ downstream of the luciferase gene ...
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bioRxiv - Cancer Biology 2022Quote: ... ATP levels were measured using the CellTiter-Glo 3-D Reagent (Promega, catalog no. G9681) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2022Quote: ... Apoptosis was assessed by commercially available caspase 3/7 assay (Cat # G8090, Promega, Madison, WI) as per the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2023Quote: ... Apoptosis activities were detected using substrate from the Caspase-3 Glo Apoptosis kit (Promega, WI).
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bioRxiv - Developmental Biology 2023Quote: ... The prospero 3’UTR [2131bp] was subcloned into the pmirGlo Dual-Luciferase expression vector (Promega) downstream of the firefly luciferase gene ...
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bioRxiv - Cell Biology 2021Quote: HEK293 T-REx SNAPf-GLP-1R-SmBiT cells were seeded in 12-well plates and co-transfected with 0.1 μg β-arrestin-2 fused at the N-terminus to LgBiT (LgBiT-β-arrestin-2; Promega, plasmid no. CS1603B118) and either 0.1 μg mini-Gs-Venus or mVenus supplemented with 0.8 μg pcDNA 3.1.
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bioRxiv - Molecular Biology 2023Quote: ... Figure 6 and Figure 7) to form condensates in cells was monitored by transient transfection of U-2 OS cells using FuGene (Promega; Figures 2 and 7) or electroporation (as before ...
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bioRxiv - Cell Biology 2020Quote: ... following manufacturer’s instructions using 200 ng psiCHECK™ 2 (Promega) as empty vector control or psiCHECK2 containing Tnf-α 3’ UTR or Il6 3’ UTR and 30 pmol miRNA scramble negative control ...
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bioRxiv - Molecular Biology 2019Quote: A commercial kit (HDAC-Glo™ 2 Assays, Promega, G9590) was used to measure HDAC2 activity ...
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A general role of zinc binding domain revealed by structures of σ28-dependent transcribing complexesbioRxiv - Molecular Biology 2020Quote: ... samples were treated with 2 U/mL DNase I (Promega) for 1 min at 37 °C ...
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bioRxiv - Biochemistry 2020Quote: ... After adding 2 μl of trypsin (0.5 μg/μl, Promega), peptide mixtures were incubated at 37°C for 4 hrs ...
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bioRxiv - Biochemistry 2020Quote: ... Before adding 2 μl of trypsin (0.05 μg/μl, Promega), 500 fmol of isotope-labeled peptides 1 and 2 were added to spike the ROS preparation ...
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bioRxiv - Cancer Biology 2020Quote: ... 2 µg of total RNA was treated with DNAse (Promega) and utilized as a template for the cDNA synthesis reaction using MMLV reverse transcriptase (Promega ...
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bioRxiv - Biochemistry 2021Quote: ... Amplified DNAs were cloned into pSiCHECK-2 (Promega, Wuhan, China) by utilizing XhoI and NotI restriction enzyme sites to construct pSi2-hUTR and pSi2-mUTR ...
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bioRxiv - Neuroscience 2020Quote: ... using the GoTaq® 2-step RT-qPCR kit (Promega). Quantitative PCR runs were performed on a 7900HT Fast Real-Time PCR System (Applied Biosystems Inc. ...
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bioRxiv - Biochemistry 2020Quote: ... T7 RNA polymerase and 2 µL RNasin RNase inhibitor (Promega) and incubated overnight at 37°C ...
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bioRxiv - Evolutionary Biology 2019Quote: ... Polymerase chain reactions were performed using 2 U Gotaq (Promega), 1X Gotaq buffer (Promega) ...
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bioRxiv - Biochemistry 2021Quote: ... 1 mM CaCl 2 and 10 ng/μL trypsin (Promega). Trypsin digestion was performed at 37 °C with rotation overnight and the beads were washed three times with PBS and distilled water again ...
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bioRxiv - Microbiology 2021Quote: ... 2 μg of 0.5 μg/μl sequencing grade trypsin (Promega) was added ...
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bioRxiv - Microbiology 2021Quote: To clone the 3xant-miR-275 into psiCheck-2 (Promega), two complementary single-stranded oligos that encode 3xant-miR-275 and XhoI and NotI restriction endonuclease cut sites were synthesized at Yale Keck Oligo Synthesis Resource (Table 1) ...
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bioRxiv - Cell Biology 2022Quote: ... the protein suspension was digested with 2 µg trypsin (Promega) overnight at 37°C ...