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Citations for Promega :
1801 - 1850 of 2707 citations for 6 METHYL 3 1H TETRAZOL 5 YL 4H CHROMEN 4 ONE since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2023Quote: ... cells were transfected with 12 µg of plasmid expressing the spike from various variants using Fugene-6 (Promega; catalog number: E2691) according to the manufacturer’s instructions and incubated at 37°C ...
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bioRxiv - Cell Biology 2023Quote: ... or pCEP4-OCT4-T234A-S235A (addgene, 40630) or pCEP4-OCT4-T234E-S235E (addgene, 40631) for 48 h using FuGENE 6 (Promega, E2691) according to the manufacturer’s protocol.
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bioRxiv - Physiology 2024Quote: ... a targeting vector was constructed by subcloning a 9.1 kb region from an Akr1b7 positively identified C57BL/6 fosmid clone (WI1-1577M19) into the 2.45 kb-iTL cloning vector derived from the pSP72 vector (Promega; Madison, WI). The region was designed so the long homology arm (LA ...
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bioRxiv - Cell Biology 2024Quote: ... Cells were transfected the following day using 0.6–1 µg of plasmid and Viafect reagent (Promega E4981, 6 µl/µg plasmid). The medium was exchanged the following day to regular culture medium ...
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bioRxiv - Microbiology 2024Quote: ... and simultaneously transfected with 0.4 μg of respective EGFP-Rab plasmids using FuGENE 6 transfection reagent (Cat. E269A, Promega, Madison, WI) per the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2024Quote: ... and gene expression was determined by quantitative PCR (QuantStudio 6 Pro Real-Time PCR System) with GoTag qPCR System (Promega, A6002). Actb and Rplp0 were used as housekeeping genes ...
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bioRxiv - Biochemistry 2019Quote: ... the concentrated peak fractions were incubated with 5 □M Halo-Alexa488 (Promega) for 10 min at room temperature ...
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bioRxiv - Cell Biology 2021Quote: To analyze the 5’ UTR variants we used the pGL3-Basic (Promega), amplification of the 1.4 kb of the 5’ UTR region was carried out using Phusion High Fidelity polymerase (ThermoFisher ...
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bioRxiv - Plant Biology 2021Quote: ... 5 µl of 5X GoTaq Flexi reaction buffer (Promega, Madison, WI, USA), 2 mM of MgCl2 ...
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bioRxiv - Cell Biology 2021Quote: ... the concentrated peak fractions were incubated with 5 μM Halo-Alexa488 (Promega) for 10 min at room temperature ...
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bioRxiv - Cancer Biology 2022Quote: ... cells were incubated with 5 μM coelenterazine h (Promega, Madison, WI, USA) in phosphate-buffered saline (PBS ...
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bioRxiv - Microbiology 2022Quote: ... 5 μL of 50 μM stock solution of TMR dye (Promega G8251) was added to the concentrated culture ...
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bioRxiv - Microbiology 2022Quote: ... 1 complete protease inhibitor tablet EDTA-free and 5 µl DNaseI (Promega), lysed using a cell disruptor and the insoluble fraction removed by centrifugation (15,000 xg ...
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bioRxiv - Molecular Biology 2019Quote: ... 5 μl reaction were mixed with 50 μl luciferase assay substrate (Promega). A Tecan SPARK 10M plate reader was used to measure luciferase activity.
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bioRxiv - Molecular Biology 2021Quote: ... 20 μl of 5 ng μl−1 trypsin (sequencing grade, Promega®) in 50 mM AHC plus 1 mM CaCl2 were added to each well and incubated for 20 min at 4°C ...
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bioRxiv - Genomics 2019Quote: ... and 2 units (5 U/μl) of GoTaq polymerase (Promega, Madison, Wisconsin). PCR products were separated following agarose gel electrophoresis ...
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bioRxiv - Cell Biology 2019Quote: ... and 5 ng/mL recombinant human basic fibroblast growth factor (bFGF, Promega) then centrifuged ...
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bioRxiv - Cell Biology 2021Quote: ... GST-dimerized dynein constructs were labeled with 5 mM Halo-TMR (Promega) in the column for 10 min at room temperature and the unbound dye was washed away with TEV buffer at 4 °C ...
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bioRxiv - Genetics 2019Quote: ... and 2 units (5 U/μl) of GoTaq polymerase (Promega, Madison, Wisconsin). The PCR amplicons were applied onto a 2% agarose gel with appropriate controls and markers.
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bioRxiv - Evolutionary Biology 2022Quote: ... 5 μl of 1.5 mM GoTaqFlexi DNA polymerase Mix (Promega no. M8305), 1 μl of genomic DNA and 2.4 μl of H2O ...
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bioRxiv - Cancer Biology 2022Quote: ... PSC proliferation was followed for 5 days with CellTiter-Glo (G7572; Promega) with measurements every 24 hours.
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bioRxiv - Cancer Biology 2023Quote: ... Viability was determined after 5 days using CellTiterGlo reagent (Cat #G9683, Promega) and detected using FluoStar Optima Plate Reader (21999 ...
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bioRxiv - Neuroscience 2023Quote: ... before introduction of 2 μl seeds (diluted 1:5) using MultiFectam (Promega), following the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2023Quote: ... The viability was measured after 5 days with CellTiter Glo 2.0 (Promega). For more details ...
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bioRxiv - Microbiology 2023Quote: ... or 5 µL qPCR plasmid pJEB029 (diluted serially in lambda DNA (Promega)) were added ...
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bioRxiv - Biochemistry 2023Quote: ... and cells were chased with HaLo-Tag TMR ligand (5 µM, Promega) in Opti-MEM for 15 min ...
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bioRxiv - Cell Biology 2023Quote: ... 5 μL culture were added to 45 μL NanoGlo reagent (1x Promega lysis buffer and NanoGlo reagent ...
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bioRxiv - Cell Biology 2023Quote: ... 5% glycerol) and Ndc80c was fluorescently labeled with HaloTag TMR ligand (Promega) for 2 h at 4°C ...
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bioRxiv - Microbiology 2024Quote: ... which included a 5 µl nucleotide mix containing 2.5 mM NTPs (Promega). For each reaction ...
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bioRxiv - Plant Biology 2020Quote: ... 3×HA-fused CabLCV MP and NSP proteins were independently expressed using the TNT system (Promega) according to the manufacturer’s recommendations ...
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bioRxiv - Molecular Biology 2020Quote: Apoptosis assays were performed using Caspase-Glo 3/7 reagent according to manufacturer’s instructions (Promega #0000239042). Cell viability assays were performed using WST-1 cell viability reagent according to manufacturer’s instructions (Sigma #18993700) ...
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bioRxiv - Neuroscience 2019Quote: ... were transfected with FLAG-tagged Nlgn1 or GFP-tagged NGL-3 using Fugene6 (Promega, Cat #E2691). Transfected HEK293T cells were mechanically dissociated 24 hr after transfection and co-cultured with DIV10 cortical neurons prepared from C57BL/6J Sorcs1flox/flox mice for 16 hr.
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bioRxiv - Developmental Biology 2019Quote: ... Nano-Luc activity was measured after 3 hours using the Nano-Glo® Luciferase Assay (Promega) and imaged with the IVIS Lumina LT system ...
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bioRxiv - Cell Biology 2021Quote: ... Caspase activation was measured using the Caspase Glo 3/7 Assay Kit (Promega, Madison, WI, USA) according to manufacturer’s instructions.
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bioRxiv - Biochemistry 2020Quote: ... cell metabolic activity or caspase 3/7 activity was measured using the CellTiter-Glo assay (Promega) and Caspase-Glo 3/7 assay (Promega) ...
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bioRxiv - Cell Biology 2021Quote: ... Cells were transfected with Galectin-3-GFP plasmid (0.5 µg) using FuGene HD (Promega, Madison, WI) with a ratio of 3:1 for 24 h at 37 °C in 5% CO2 ...
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bioRxiv - Cancer Biology 2021Quote: ... wild type or mutant 3′-UTR of DKK3 was cloned into the psicheck-2 vector (Promega). HCT116 cells were transfected with and wild-type or mutant 3′-UTR-luc by using Lipofectamine 3000 ...
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bioRxiv - Evolutionary Biology 2022Quote: ... Tryptic digestion was performed for 3 h with 0.1 μg trypsin (Promega Sequencing Grade; Madison, WI) per sample ...
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bioRxiv - Plant Biology 2021Quote: ... 2-3 μg of the purified RNA was in vitro translated using wheat germ extract (Promega) at 25 °C for 2 h as per the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2020Quote: ... WT or mutant promoter of human Rpl28 gene was cloned into pGL-3-basic (Promega, E1751). The number is relative to transcriptional start site ...
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bioRxiv - Biophysics 2020Quote: ... 3 μL of translation reaction was added to 30 μL Bright-Glo Luciferase assay reagent (Promega) and the resulting luminescence was measured in the illuminometer over a spectral wavelength of 350–650 nm and an integration time of 10 s at room temperature ...
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bioRxiv - Cancer Biology 2020Quote: ... Luminescence was determined after 3 days of culture using the GloMax® microplate reader (GM3000, Promega) and normalized with seeding control plate.
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bioRxiv - Microbiology 2021Quote: ... Caspase activity was determined using the Caspase-Glo 3/7 assay system (Promega, Madison, WI, USA) according to the manufacturer’s instruction ...
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bioRxiv - Microbiology 2021Quote: ... Infectivity was determined after 3 days by luminescence following addition of BrightGlo reagent (Promega PR-E2620). IC50 were calculated by nonlinear regression analysis using GraphPad Prism (version 9.0 ...
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bioRxiv - Microbiology 2022Quote: Caspase 3/7 activity was measured using the Caspase-Glo assay kit (Promega, Madison, WI, USA), according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2022Quote: Caspase 3/7 activity and cell viability were determined by ApoTox-Glo assay kit (G6321, Promega). Briefly ...
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bioRxiv - Microbiology 2022Quote: ... the second set of plates was analyzed by adding CellTiter-Glo 2.0 Reagent (Promega, G9242/3) and measuring luminescence on a GloMax Discover System (Promega) ...
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bioRxiv - Molecular Biology 2022Quote: The 3’UTR of the genes of interest was cloned in the pSI-Check2 vector (Promega) using the primers listed in Supplementary Table S6 ...
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bioRxiv - Microbiology 2023Quote: ... following tryptic digest at 37 °C for 3 h in the same buffer with trypsin (Promega) in a 1:30 ratio (w/w ...
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bioRxiv - Microbiology 2023Quote: ... the cells were co-transfected using Fugene HD transfecting reagent at a 1:3 ratio (Promega) along with pRL-TK Renilla (Renilla Luciferase ...