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Citations for Promega :
1801 - 1850 of 4574 citations for 6 CHLORO 2 METHYLIMIDAZO 1 2 A PYRIDINE 3 CARBOXYLIC ACID since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2021Quote: ... Viral RNA was extracted from a 300 μL plasma aliquot using the Viral Total Nucleic Acid Kit (Promega, Madison, WI) on a Maxwell 16 MDx or Maxwell RSC 48 instrument (Promega ...
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bioRxiv - Microbiology 2020Quote: ... Viral RNA was extracted from a 300 μL plasma aliquot using the Viral Total Nucleic Acid Kit (Promega. Madison. WI) on a Maxwell 16 MDx or Maxwell RSC 48 instrument (Promega ...
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bioRxiv - Immunology 2023Quote: ... viral RNA (vRNA) was isolated from plasma samples using the Maxwell Viral Total Nucleic Acid Purification kit (Promega, Madison WI). Next ...
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bioRxiv - Microbiology 2023Quote: ... were made by replacing the ORF3 region encoding 5–105 amino acids with an in-frame insertion downstream of 4th VP2 codon sequence of UnaG and NanoLuc (Promega) coding regions with their own stop codons ...
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bioRxiv - Plant Biology 2023Quote: The HLB1 coding sequence corresponding to the N-terminal 200 amino acids was amplified by PCR using the following primers and was cloned into pGEM-T-easy vector (Promega). The NdeI and XhoI fragment of HLB11-200 was cloned into pET28a vector (Novagen ...
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bioRxiv - Molecular Biology 2023Quote: ... RNA was isolated with the Maxwell RSC viral total nucleic acid purification kit on a Maxwell RSC 48 instrument (Promega). Isolated ZIKV RNA was titrated by qRT-PCR using TaqMan Fast virus 1-step master mix (ThermoFisher ...
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bioRxiv - Biochemistry 2022Quote: ... 1200 ng of ORs tagged with the first 20 amino acids of human rhodopsin (rho-tag) at the N-terminal ends53 in pCI (Promega) and 30 ng eGFP were transfected using Lipofectamine 2000 (Invitrogen ...
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bioRxiv - Neuroscience 2023Quote: ... 1,200 ng of ORs tagged with the first 20 amino acids of human rhodopsin (rho-tag) at the N-terminal ends in pCI mammalian expression vector (Promega) and 30 ng eGFP were transfected using Lipofectamine 2000 (11668019 ...
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bioRxiv - Immunology 2024Quote: ... Ten to twenty 10 µm cryosections were used for nucleic acid extraction using the automated Maxwell® RSC instrument (Promega) with the appropriate sample kit and according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2021Quote: ... HEK293TN cells were seeded in 6-well plates and transiently transfected with plasmid of choice using FuGene transfection reagent (Promega) and OptiMem (Gibco) ...
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bioRxiv - Cancer Biology 2021Quote: ... S2-CP8 cells were then transiently transfected with both mRFP-FKBP-5-ptase-dom and PM-FRB-CFP (0.5 μg/well of a 6-well plate for each vector) with ViaFect (Promega Corp.). After 24 h culture ...
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bioRxiv - Neuroscience 2021Quote: ... coated 12-well plate and transfected the next day using the Fugene 6 transfection reagent and according to the manufacturer’s directions (Promega E2691). The same amount of plasmid was transfected per condition ...
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bioRxiv - Neuroscience 2021Quote: ... 100 mm cell culture dishes were seeded with cells 24 hours prior to transfection and transfected the following day with the pCAGIG-Emx2 plasmid using FuGENE 6 transfection reagent (Promega) as per the manufacturer’s recommendations ...
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bioRxiv - Cancer Biology 2021Quote: ... 500 ng of lentiviral plasmid encoding doxycycline inducible transporters and 500 ng of each of the packaging plasmids were transfected into HEK 293T cells with FuGENE 6 transfection reagent (Promega). Cell supernatant was replaced 24 h after transfection and harvested after another 24 h ...
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bioRxiv - Cell Biology 2019Quote: ... Transfections were performed with 4 μg of DNA, 800 μL of OptiMEM (Invitrogen, and 12 μL of FuGENE 6 transfection reagent (Promega) per 10 cm dish ...
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bioRxiv - Molecular Biology 2019Quote: ... The cells were then stimulated with 1 ng/mL of IL-1β for 6 h and subsequently washed with ice-cold PBS and lysed in Passive Lysis Buffer (PLB; Promega). Luciferase activity was measured in a Clariostar plate reader (BMG Biotech ...
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bioRxiv - Neuroscience 2019Quote: ... the whole brain of postnatal day 6 (P6) animals or dorsal raphe tissue punches (adult animals) were homogenized with Passive Lysis Buffer (Promega) and proteinase inhibitors (cOmplete ULTRA Tablets ...
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bioRxiv - Biochemistry 2019Quote: ... 500 ng of the lentiviral backbone plasmid containing the guide sequence and 500 ng of each of the packaging plasmids were transfected into HEK 293T cells using FuGENE 6 transfection reagent (Promega). The viral media was exchanged after 24 hours and was harvested after 48 hours ...
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bioRxiv - Biochemistry 2019Quote: ... These cells were then transfected with 50 ng of pcDNA3-STING-HA and 500 ng of either pcDNA3-FLAG-HA or pcDNA3-FLAG-HA-SLC19A1 using FuGENE 6 transfection reagent (Promega). After 24 h ...
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bioRxiv - Cancer Biology 2019Quote: Replication-deficient retro- and lentiviral particles were produced by transient transfection of 293T cells using FuGENE® 6 reagent (Promega). In brief ...
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bioRxiv - Biochemistry 2020Quote: ... 500 ng of the lentiviral backbone plasmid containing the guide sequence and 500 ng of each of the packaging plasmids were transfected into HEK 293T cells using FuGENE 6 transfection reagent (Promega). The viral media was exchanged after 24 hours ...
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bioRxiv - Neuroscience 2020Quote: ... and a pFUGW vector containing a Cre-recombinase and/or vGluT1-pHluorin construct using the Fugene 6 transfection reagent (Promega). Cell culture supernatants containing the virus were collected 72 hours later and spun down to precipitate out cellular debris and other contaminants ...
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bioRxiv - Cancer Biology 2020Quote: ... 5 and 6 d using a CellTiter 96® AQueous One Solution Cell Proliferation Assay (MTS) (G3582; Promega, Madison, WI). Cells were incubated with the MTS reagent 3-4 h ...
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bioRxiv - Microbiology 2021Quote: ... or chimeric infectious clone plasmid was transfected into C6/36 or 293T cells by FuGENE 6 transfection reagent (Promega, USA). At 72 h post-transfection ...
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bioRxiv - Molecular Biology 2020Quote: ... Protein samples were re-solubilized in 6 M urea buffer followed by reduction and alkylation before digestion with trypsin (Promega) at 37°C for 18 hr ...
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bioRxiv - Biophysics 2020Quote: ... mass ratio) into LentiX cells grown to approximately 80% confluency in 6-well plates using FuGENE HD Transfection Reagent (Promega) per manufacturer’s protocol ...
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bioRxiv - Cell Biology 2022Quote: ... RPE1 wild type cells were transfected with PX459 plasmid containing the corresponding guide sequence for 24 hours using FuGENE 6 (Promega). Following 72 hours of 4 μM Puromycin selection ...
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bioRxiv - Molecular Biology 2022Quote: ... were co-transfected with psPAX2 and pCMV-VSV-G packaging plasmids into HEK293T cells using FuGENE 6 (Promega, WI, USA). Supernatants containing virus were collected at 48h and 72h after transfection ...
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Combining LSD1 and JAK-STAT inhibition targets Down syndrome-associated myeloid leukemia at its corebioRxiv - Cancer Biology 2022Quote: ... Cell viability was determined on day 6 using the CellTiter-Glo® 2.0 Cell Viability Assay (Promega, Madison, WI, USA) according to the manufacturer’s recommendations ...
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bioRxiv - Biochemistry 2022Quote: ... The samples were then diluted to 6 M urea with 50 mM Tris-HCl pH 8 and Trypsin/Lys-C mix (Promega) was added to an enzyme:substrate ratio of 1:25 (w/w ...
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bioRxiv - Biophysics 2022Quote: ... mass ratio) into LentiX cells grown to approximately 80% confluency in 6-well plates using FuGENE HD Transfection Reagent (Promega) per manufacturer’s protocol ...
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bioRxiv - Cell Biology 2022Quote: hCO viability after 6 days of doxo treatment was assessed by CellTiter-Glo® Luminescent Cell Viability Assay Kit (Promega) according to manufacturer’s instructions ...
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bioRxiv - Microbiology 2022Quote: ... CrPV infection was performed at MOI 20 for 6 hours and then cells were lysed in passive lysis buffer (Promega) and luciferase activities measured by a dual-luciferase assay (Promega).
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bioRxiv - Biochemistry 2019Quote: A commercial K562 cell digest supplemented with the 6 x 5 LC-MS/MS Peptide Reference Mix (QC4Life Reference Standard, Promega) was used as quality control (QC ...
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bioRxiv - Cancer Biology 2019Quote: ... Protein precipitation was done using 6 volume of ice cold acetone and digested overnight at 37 o C using modified trypsin (Promega). Then peptides were fractionated by bRPLC and 8 fractions were collected ...
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bioRxiv - Biochemistry 2019Quote: ... After 24 hours cells were transfected with pcDNA3 plasmids containing ΔCTT STING-FLAG variants with or without co-transfection with pcDNA3 CTT-HA plasmids using Fugene 6 Transfection Regent (Promega). After 24 additional hours cells were lysed in mild detergent (10% glycerol 25mM NaCl ...
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bioRxiv - Molecular Biology 2019Quote: ... 100,000 cells per well we seeded on glass coverslips in 6-well plates and transiently transfected after 24 hours using Fugene6 (Promega) according to manufacturer’s protocol ...
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bioRxiv - Physiology 2020Quote: ... Cells were harvested 6 h after stimulation and luciferase activity was measured according to the manufacturer’s protocol (Promega, Madison, WI). Relative luciferase activity was normalized to the β-galactosidase activity measured using multi-mode plate reader (SpectraMax M5 ...
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bioRxiv - Cell Biology 2021Quote: ... BOSC 23 retroviral packaging cells were ∼70% confluent when transfected with 12 µg of pBABE-mPPARγ2 or pBABE-EGFP plasmid DNA using Fugene 6 transfection reagent (Promega, E2311). Media containing secreted retrovirus was collected 72 hours post-transfection and filtered through 0.45 µm syringe filters ...
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bioRxiv - Microbiology 2019Quote: ... 293T cells stably expressing HA-tagged PPP2R5B or APOBEC3G were transfected with 200 ng/well control or Vif expression vector in 24-well plates using FuGENE 6 (Promega). After 36 hr ...
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bioRxiv - Microbiology 2020Quote: ... HEK293T cells were grown to 80% confluency before transfection with pCMV3-SARS-CoV2-spike (kindly provided by Dr. Peihui Wang, Shandong University, China) using FuGENE 6 (Promega). Cells were cultured overnight at 37°C with 5% CO2 ...
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bioRxiv - Developmental Biology 2019Quote: ... cells into micro-tubes containing 5 µl of cold 1X SuperScript IV VILO Master Mix for two-step RT-qPCR containing 6 units RNasin (Promega) and 0.5% (v/v ...
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bioRxiv - Biochemistry 2021Quote: ... we measured the level of accumulated 2-deoxyglucose-6-phosphate (2DG6P) using the assay kit according to the manufacturer’s protocol using a GloMax®96 microplate luminometer (Promega).
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bioRxiv - Biochemistry 2020Quote: ... cells were transfected with empty pRK5 vector or pRK5 expressing HA-UB (HA-tagged ubiquitin) by using Fugene 6 (Promega), as per the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2020Quote: ... the MCF-7 and MDA-MB-231 cells were infected with Plasmids expressing RFP or GFP using Fugene 6 (Promega) at an early passage and were selected using 2 μg/ml puromycin (Sigma).
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bioRxiv - Cancer Biology 2021Quote: ... Retrovirus was generated by transfecting SETBP1 pMSCV-IRES-mCherry or CSF3R pMSCV-IRES-GFP or control vector plasmids and the EcoPac plasmid (provided by Dr. Rick Van Etten) into the 293T17 cells using FuGENE 6 (Promega). Viral supernatants were harvested 48 and 72 hours later ...
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bioRxiv - Microbiology 2020Quote: ... HEK293T cells were seeded in T75 tissue culture flasks to be ∼80% confluent the following day and were transfected with the plasmid of interest using Fugene 6 (Promega). After ∼18 h the transfection media was removed and the cells were infected with VSVΔG∗rLuc at a multiplicity of infection of ∼0.07 for 1 h at 37°C ...
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bioRxiv - Microbiology 2020Quote: ... HEK293T cells were grown to 80% confluency before transfection with pCMV3-SARS-CoV2-spike (kindly provided by Dr. Peihui Wang, Shandong University, China) using FuGENE 6 (Promega). Cells were cultured overnight at 37°C with 5% CO2 ...
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bioRxiv - Cancer Biology 2020Quote: ... digestion was continued by incubation of the sample at 37°C for 6 hours with trypsin (Promega, Catalog Number V5113) at an enzyme to substrate ratio of 1:75 ...
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bioRxiv - Cancer Biology 2022Quote: ... the resulting plasmid and the IF-GFP-ATRX plasmid (a gift from Michael Dyer43, addgene plasmid #45444; exon 6 absent) were digested with XhoI (Promega) and SpeI (Promega ...