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Citations for Promega :
1751 - 1800 of 4746 citations for Mouse Switch associated protein 70 SWAP70 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Systems Biology 2019Quote: ... The samples were then diluted with 0.1 M NH4HCO3 to a final concentration of 1 M urea and the proteins were digested with sequencing-grade porcine trypsin (Promega, Switzerland) at a final enzyme:substrate ratio of 1:100 (w/w) ...
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bioRxiv - Biochemistry 2020Quote: ... mRNA for each chimeric Lep protein was produced from plasmids using the RiboMAX RNA production system in accordance with the manufacturer’s instructions (Promega, Madison, WI). Lep proteins were then produced from mRNA by in vitro translation using rabbit reticulocyte lysate (Promega ...
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bioRxiv - Synthetic Biology 2021Quote: All wheat germ cell-free expression used the TNT® SP6 High-Yield Wheat Germ Protein Expression System L3260/L3261 (Promega) according to manufacturers’ instructions ...
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bioRxiv - Cell Biology 2021Quote: ... Myc tagged full-length and truncated ARL13B fragments were generated by in vitro translation using TNT Sp6 high-yield wheat germ protein expression system (Promega; L3261) from pCS2-Myc-ARL13B vectors generated by gateway cloning ...
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bioRxiv - Cancer Biology 2022Quote: ... Proteins were alkylated using 15 mM chloroacetamide at 37° C for 30 min and further digested using sequencing-grade modified trypsin (1/50 w/w, ratio trypsin/protein; Promega, USA) at 37° C for 12 hours ...
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Reducing mitochondrial ribosomal gene expression does not alter metabolic health or lifespan in micebioRxiv - Cell Biology 2022Quote: ... The urea concentration was reduced to <2M and the protein sample was digested overnight at 37°C with 1:100 Trypsin Gold (Promega V5280). The following day ...
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bioRxiv - Plant Biology 2022Quote: ... PtaMYC2.2 and PtaJAM1.1 were cloned into pIXHALO (ABRC, Ohio State University) and expressed in vitro using TnT® SP6 High-Yield Wheat Germ Protein Expression System (Promega). We used empty vector pIXHALO as control ...
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bioRxiv - Physiology 2022Quote: ... Protein concentration in the supernatant was measured using a NanoDrop and 1:200 (w/w) of trypsin (Promega, Mass Spectrometry grade) was added to 200 µg of sample ...
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bioRxiv - Cell Biology 2022Quote: After extraction of proteins (see above) without proteinase and phosphatase inhibitors the Src activity was evaluated with ProFluor® Src-Family Kinase Assay (Promega).
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bioRxiv - Molecular Biology 2023Quote: ... Then, the proteins were purified by acetone precipitation and digested with LysC (FUJIFILM Wako, Osaka, Japan) and trypsin (Promega, Madison, WI). The resulting peptides were desalted on InertSep RP-C18 columns (GL Sciences ...
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bioRxiv - Cell Biology 2023Quote: ... Proteins were alkylated using 15 mM chloroacetamide at 37° C for 30min and further digested using sequencing-grade modified trypsin (1/50 w/w, ratio trypsin/protein; Promega, USA) at 37°C for 12h ...
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bioRxiv - Molecular Biology 2023Quote: ... Western blots were run according to standard procedure and probed with streptavidin IRDye680RD (Licor 925-68079) to track biotinylated HaloTag protein and anti-HaloTag antibody (Promega G928A) to track total HaloTag protein ...
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bioRxiv - Molecular Biology 2023Quote: Western blots were run according to standard procedure and probed with streptavidin IRDye680RD (Licor 925-68079to track biotinylated HaloTag protein and anti-HaloTag antibody (Promega G928A) to track total HaloTag protein ...
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bioRxiv - Microbiology 2023Quote: ... The presence of glycosylated proteins in the cell lysates was assessed using Promega Endoglycosidase H (Endo H) assay reagents (Promega V4871). Briefly ...
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bioRxiv - Microbiology 2023Quote: ... The quantity of the reporter protein was examined by measuring its enzymatic activity through the Renilla Luciferase Assay System (Promega, Germany), as per manufacturer’s instruction ...
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bioRxiv - Plant Biology 2024Quote: Protein expression was carried out using the in vitro transcription and translation system (TnT™ T7 Quick for PCR DNA, Promega) and the EMSA reaction was carried out following manufacturer’s instruction (odyssey) ...
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bioRxiv - Biochemistry 2023Quote: ... Bait proteins encoded by pIX-Halo were expressed using the TNT T7-coupled wheat germ extract system (Promega, cat. no. L4140) according to the manufacturer’s recommendations ...
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bioRxiv - Biochemistry 2023Quote: The FASP method[7] was used to digest urine protein with trypsin (Trypsin Gold, Mass Spec Grade, Promega, Fitchburg, Wisconsin, USA). One hundred micrograms of urine protein was added in the membrane of a 10KD ultrafiltration tube (Pall ...
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bioRxiv - Biochemistry 2023Quote: ... samples were resuspended in 6 M guanidine hydrochloride.250 µg of protein were digested overnight at 37 °C using trypsin with a 1:20 trypsin to sample ratio (Promega, #V5117). The next day ...
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bioRxiv - Microbiology 2023Quote: ... The supernatant (700 μL) was transferred to a sterile Eppendorf tube and mixed with 50 μL protein precipitation solution (Promega, USA) by inverting it ten times before centrifugation (10 min ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... Assessment of total cellular HiBiT-GCase protein abundance was performed using the Nano-Glo® HiBiT Lytic Detection System (Cat.#N3040, Promega), according to the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2024Quote: ... The antiHER2 protein was produced from CD81-antiHER2-tGFP vector (600 ng) using TNT® Quick Coupled Transcription/Translation Systems (Promega), following manufacturer’s instructions ...
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bioRxiv - Neuroscience 2021Quote: ... using CalPhos™ Mammalian Transfection Kit (Takara Bio or Promega) on DIV7 ...
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bioRxiv - Evolutionary Biology 2020Quote: ... the PCR products were cloned using the pGEMT kit (Promega): 3 μl PCR products were ligated to 50 ng pGEMT vector in the presence of 3 units T4 ligase overnight at 18°C (final volume 10 μL) ...
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bioRxiv - Evolutionary Biology 2021Quote: ... Locusta cDNA was synthesized using the cDNA Synthesis Kit (Promega). 2.5 ng of cDNA was used in each qPCR reaction ...
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bioRxiv - Molecular Biology 2021Quote: ... An ImProm-II™ Reverse Transcriptase System kit (Promega, USA) was used to synthesize the first strand cDNA using 1.5 μg of the DNA-free RNA sample as a template and each reaction was carried out according to the supplier’s protocol ...
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bioRxiv - Evolutionary Biology 2022Quote: ... cDNA was prepared using Promega kit (REF 017319, Promega, USA). Primers were designed according to the sequence information from the NCBI database using Primer3 Input (version V ...
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bioRxiv - Genomics 2020Quote: ... Assays were performed using the dualluciferase reporter assay kit (Promega) and a Veritas Microplate Luminometer (Turner Biosystems) ...
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bioRxiv - Immunology 2021Quote: The T Cell Activation Bioassay kit was purchased from Promega. The Jurkat-NFAT cells were thawed and cultured in RPMI (supplemented with 10% FBS and 5% pen/strep ...
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bioRxiv - Immunology 2021Quote: ... cDNA was then synthesized using AMV reverse transcriptase kit (Promega) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2019Quote: ... coli S30 Extract System for Linear Templates Kit (Promega #L1030) as previously described (C ...
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bioRxiv - Cell Biology 2019Quote: ... purified chromatin was recovered using a PCR purification kit (Promega). Sequencing libraries were generated using standard methods and samples were sequenced on a MiniSeq instrument (Illumina ...
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bioRxiv - Molecular Biology 2019Quote: A commercial kit (HDAC-Glo™ 2 Assays, Promega, G9590) was used to measure HDAC2 activity ...
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bioRxiv - Zoology 2019Quote: ... purified using Wizard SV Gel and PCR Cleanup kit (Promega), and sequenced directly (Sequetech ...
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bioRxiv - Microbiology 2019Quote: ... The Wizard genome DNA purification kit (Promega Corp., Madison, WI) was used for isolation of bacterial genomic DNA (gDNA) ...
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bioRxiv - Immunology 2019Quote: ... cDNA was generating using the GoScript Reverse Transcriptase kit (Promega). Quantitative-PCR was set up using the SensiFAST SYBR Hi-ROX kit (Bioline ...
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bioRxiv - Evolutionary Biology 2020Quote: ... using the Dual-Glo Luciferase Assay System Kit (Promega, US) as substrate ...
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bioRxiv - Microbiology 2020Quote: ... cDNA was then synthesized using AMV reverse transcriptase kit (Promega) with Oligo-dT primer ...
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bioRxiv - Bioengineering 2021Quote: ... and collagen content via a QuantiFlour dsDNA assay kit (Promega), a modified 1,9-dimethylmethylene blue (Sigma-Aldrich ...
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bioRxiv - Neuroscience 2020Quote: ... the transfection was conducted using FuGENE6 kit (Cat#2619, Promega) according to the manufacturer’s protocol.
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bioRxiv - Cancer Biology 2020Quote: ... Luciferase assays were performed using Dual Luciferase Assay Kit (Promega) as described (Malecki et al. ...
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bioRxiv - Cell Biology 2021Quote: ... dsRNA was then synthesized using the T7 Ribomax kit (Promega) as instructed by the manufacturer ...
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bioRxiv - Plant Biology 2020Quote: ... using the GoTaq® Flexi DNA Polymerase kit (Promega, USA). Reaction volumes and thermocycling profiles were set according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: Genomic DNA was extracted using the Wizard Genomic Kit (Promega), and sequenced by PacBio (Earlham Institute ...
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bioRxiv - Neuroscience 2020Quote: ... using the GoTaq® 2-step RT-qPCR kit (Promega). Quantitative PCR runs were performed on a 7900HT Fast Real-Time PCR System (Applied Biosystems Inc. ...
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bioRxiv - Microbiology 2021Quote: ... and processed using the GSH-Glo Glutathione Assay kit (Promega) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... The Wizard genome DNA purification kit (Promega Corp., Madison, WI) was used for isolation of bacterial genomic DNA (gDNA) ...
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bioRxiv - Microbiology 2020Quote: ... cDNA was generated using the GoScriptTM reverse transcriptase kit (Promega) from RNA (250 ng ...
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bioRxiv - Cancer Biology 2020Quote: ... and fingerprinted using a PowerPlex 1.2 kit (Promega, Madison, WI) to confirm the cell line identity.
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bioRxiv - Cancer Biology 2020Quote: ... Luciferase assays were performed using a luciferase assay kit (Promega) according to the manufacturer’s instructions ...