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Citations for Promega :
1751 - 1800 of 5086 citations for Human Guanylate Binding Protein 6 GBP6 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2021Quote: ... Proteins were extracted using 100 μL of Passive Lysis Buffer from Dual-Luciferase reporter assay systems (Promega, E1980) according to manufacturer’s instruction and assayed using FLUOstar Omega (BMG Labtech) ...
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bioRxiv - Biophysics 2019Quote: ... Proteasomes were affinity purified with the bait protein GST-Ubl and magnetic beads (MagneGST™ Glutathione Particles, Promega) and eluted with purification buffer containing 25 mM reduced L-glutathione ...
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bioRxiv - Microbiology 2022Quote: ... Proteins on magnetic beads were digested overnight at 37°C with 1μl (0.2 μg/μL) of trypsin (Promega) in a 25-mM NH4HCO3 buffer per sample ...
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bioRxiv - Biochemistry 2020Quote: ... Lep proteins were then produced from mRNA by in vitro translation using rabbit reticulocyte lysate (Promega, Madison, WI) supplemented with canine pancreatic rough microsomes (tRNA probes ...
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bioRxiv - Cancer Biology 2019Quote: ... Dilute proteins were digested overnight with 5 μl of 25 ng/μl trypsin gold (Promega, Madison, WI, USA) and desalted with a ZipTipC18 pipette tip (Millipore ...
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bioRxiv - Molecular Biology 2019Quote: ... and PERP plus any interacting proteins were cleaved from the resin using 30 units of ProTEV enzyme (Promega) at room temperature for one hour (elution 1) ...
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bioRxiv - Neuroscience 2019Quote: ... Resins were transferred to a new tube and proteins were digested with 0.1 µg Lys-C for 2h at 37°C and 0.5 µg trypsin (Promega) overnight at 37°C ...
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bioRxiv - Microbiology 2020Quote: The protein samples were digested with 500 ng of sequencing grade trypsin (V511, Promega Co., Madison, WI, USA) and incubated at 37 °C following the protocol described by Shevchenko et al.22 ...
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bioRxiv - Plant Biology 2020Quote: ... LUC activity was measured with 20 μl of protein sample and 100 μl of LUC assay reagent (Promega). Activation from the DHS was calculated as (LUC luminescence/rate of MUG accumulation ...
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bioRxiv - Cell Biology 2020Quote: ... The proteins were then digested overnight at 37°C with 0.2 μg of modified sequencing grade trypsin (Promega) in 50 mM ammonium bicarbonate ...
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bioRxiv - Cell Biology 2021Quote: ... Proteins were digested on the column with 0.25 µg of Trypsin/Lys-C protease mix (Promega; cat. V5071), and peptides eluted for mass spectrometry.
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bioRxiv - Microbiology 2019Quote: ... The membranes were washed with TBST and incubated with 200-fold diluted LgBiT protein in TBST (N2421, Promega), for 1 hour at room temperature ...
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bioRxiv - Cell Biology 2021Quote: ... Halo-tagged proteins were labeled by incubating cells with 200nM HaloTag Ligand conjugated to Janelia Fluor 646 (Promega) for 30 min before imaging ...
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bioRxiv - Cell Biology 2021Quote: The Rosa26 promoter and the 5’UTRs of secretory granule protein transcripts were subcloned into the pNL1.1 (Promega) using standard cloning techniques ...
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ALiCE®: A versatile, high yielding and scalable eukaryotic cell-free protein synthesis (CFPS) systembioRxiv - Biochemistry 2022Quote: ... purified protein samples were prepared by S-alkylation with iodoacetamide and digestion with either with LysC/GluC (Promega) [RBD] or ...
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bioRxiv - Biochemistry 2022Quote: ... laevis TRAIP that was produced in the TnT® SP6 High-Yield Wheat Germ Protein Expression System (Promega) according to manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2022Quote: ... The HaloTag-CpOGA proteins were mixed with equal molar of HaloTag Alexa Fluor 488 or 660 ligands (Promega) and incubated at room temperature for 30 min ...
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bioRxiv - Physiology 2022Quote: ... Luciferase activities were measured and plotted relative to lysate protein concentrations using the Promega Dual Luciferase Reporter (Promega) assays according to the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2022Quote: ... Protein bands were detected with horseradish peroxidase (HRP)-conjugated rabbit or mouse secondary antibody [1:1,000 dilution] (Promega). The chemiluminescence signal was imaged using an Odyssey XF Imager (LI-COR ...
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bioRxiv - Neuroscience 2023Quote: ... Digestion reaction for proteins was initially set up with 1 μg of MS grade LysC (Promega, Cat# PI90307) for 3 hours at RT and then continued overnight with addition of 2 μg of trypsin (Promega ...
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bioRxiv - Cell Biology 2023Quote: ... the gel plugs were digested by using 1:50 (Trypsin: Protein amount ratio) Sequencing Grade Modified Trypsin (Promega) at 37 °C overnight ...
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bioRxiv - Molecular Biology 2023Quote: ... proteins were directly digested by incubation with sequencing-grade modified trypsin (1/50, w/w; Promega, Madison, Wisconsin) overnight at 37°C ...
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bioRxiv - Systems Biology 2023Quote: ... Samples were then digested by a 1:50 (trypsin to protein) ratio of sequencing grade modified trypsin (Promega) for 16 hours at 600 rpm and 25°C ...
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bioRxiv - Cell Biology 2023Quote: ... Proteins were digested in 100 mM ammonium bicarbonate containing 2 μg of trypsin/Lys-C enzyme mix (Promega) at 37 °C overnight ...
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bioRxiv - Molecular Biology 2023Quote: Reduced and alkylated proteins were prepared by in-gel digesting overnight at 37°C using 100ng trypsin (Promega). The supernatant was dried by vacuum centrifugation and the samples were resuspended in 0.1% trifluoro acetic acid ...
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bioRxiv - Plant Biology 2023Quote: ... 1 μg protein was used for dual luciferase transactivation assay using Dual-Luciferase® Reporter Assay System (Promega) following manufacturer’s protocol ...
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bioRxiv - Neuroscience 2023Quote: ... immunodepleted protein samples were successively digested with LysC (0.008 μg/μL, Wako) and trypsin (0.002 μg/μL, Gold, Promega), using a filter-aided sample preparation (FASP ...
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bioRxiv - Plant Biology 2023Quote: ... In-gel digestion of proteins was performed at 37 °C overnight using 2.5 μg Trypsin/Lys-C (Promega). Samples were further digested with 2.5 μg Chymotrypsin (Promega ...
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bioRxiv - Plant Biology 2023Quote: ... LUC activity was measured with 20 mL of protein sample and 100 mL of LUC assay reagent (Promega). Promoter activation was calculated as (rate of MUG accumulation / LUC luminescence ...
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bioRxiv - Plant Biology 2024Quote: ... flag-tagged MED25 protein and CFP-tagged RAP2.2 and RAP2.12 proteins (Schmidt et al., 2018) were expressed using TNT SP6 High Yield Wheat Germ (Promega) following the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2024Quote: ... 30 µg of proteins from lysed cells were digested with 500 units of endoglycosidase H (ENDO H, Promega) and 10 units of Peptide N-glycosidase F (PNGase F ...
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bioRxiv - Genomics 2020Quote: DNA was extracted from the fin clips of the challenged fish using a commercial kit (Wizard Genomic DNA Purification Kit, Promega), following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2019Quote: ... Genomic DNA from Legionella was isolated using the Wizard Genomic DNA Purification Kit or the Maxwell DNA extraction kit (Promega). Plasmids from L ...
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bioRxiv - Genomics 2019Quote: ... The genomic DNA and total RNA were extracted from cells using the Maxwell 16 Cell LEV DNA Purification Kit and the Maxwell 16 Cell LEV Total RNA Purification Kit (Promega), respectively.
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bioRxiv - Molecular Biology 2019Quote: ... collected by scraping and lysed in the buffer provided by the Nucleospin RNA isolation kit (Mackerey-Nagel) or the Reliaprep miniprep kit (Promega). RNA was isolated according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: ... paraffin-embedded (FFPE) tissue slides using Maxwell® RSC Blood DNA Kit and Maxwell® RSC RNA FFPE Kit (Promega) following the manufacturer’s recommendations ...
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bioRxiv - Plant Biology 2022Quote: ... purified with a NucleoSpin® Gel & PCR Clean-up kit (Machery-Nagel) and radiolabeled dCTP-P32 were incorporated with a Prime-a-gene Labeling System kit (U1100, Promega). Oligonucletotide probes were ordered from GenoScreen and radiolabeled dATP-P32 were incorporated with a T4 Polynucleotide Kinase kit (T4 PNK EK0031 ...
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bioRxiv - Microbiology 2023Quote: ... gallolyticus genomic DNA was extracted using either Wizard Genomic DNA Purification Kit or Wizard SV Genomic DNA Purification Kit (Promega). The extraction was performed according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2024Quote: Total RNA was extracted from FACS purified iPSC-neurons and frozen mouse brain tissue using the ReliaPrep RNA cell kit and ReliaPrep RNA tissue extraction kit (Promega) respectively.
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bioRxiv - Plant Biology 2020Quote: ... carried out using the RQ1-DNase kit (Promega), and were reverse transcribed into cDNA using the iScript™ cDNA Synthesis Kit (Biorad) ...
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bioRxiv - Plant Biology 2020Quote: ... using the Wizard Genomic DNA Purification Kit (Promega). Cytosine methylation on target loci was assessed by Methylation-Sensitive Restriction Enzyme qPCR (MSRE-qPCR ...
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bioRxiv - Microbiology 2020Quote: Wizard Promega DNA extraction kits (Promega, Madison, WI) was used to extract DNA from overnight broth culture ...
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bioRxiv - Cell Biology 2020Quote: ... and purified using RNeasy MinElute Cleanup Kit (Promega) following standard protocols ...
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bioRxiv - Immunology 2021Quote: ... using the GoTaq qPCR Master Mix kit (Promega). Gene expression was normalized to the housekeeping gene Rplp0 and expressed as fold change compared to CD11cWT samples ...
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bioRxiv - Genomics 2020Quote: ... with the Wizard Genomic DNA Purification Kit (Promega), following manufacturer’s instructions ...
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bioRxiv - Genomics 2020Quote: ... The RealTime-Glo Annexin V Apoptosis kit (Promega) was used as instructed to quantify the degree of apoptosis in HCASMC ...
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bioRxiv - Neuroscience 2021Quote: ... purified with Wizard Genomic DNA Purification kit (Promega) and confirmed via sequencing (GeneWiz) ...
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bioRxiv - Evolutionary Biology 2019Quote: The Wizard® Genomic DNA Purification kit (Promega) was used to isolated genomic DNA from overnight cultures ...
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bioRxiv - Microbiology 2019Quote: ... using the LEV DNA kit (AS1290, Promega, USA). Briefly ...
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bioRxiv - Cell Biology 2020Quote: ... Dual-Glo® Luciferase Assay System Kit (Promega) was used according to the manufacturer’s instructions ...