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Citations for Promega :
1651 - 1700 of 4458 citations for Cow Protein L Isoaspartate PCMT1 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2019Quote: ... the expression of the fusion protein was initiated by washing out Dox and changing the medium into CDMi containing protected furimazine (Promega; 1 μM). After the NLuc signal was confirmed 5-8 hours later ...
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bioRxiv - Biochemistry 2019Quote: ... A total of 100 µg of each protein sample was digested with trypsin (Trypsin Gold, Mass Spec Grade, Promega, Fitchburg, Wisconsin, USA) by using filter-aided sample preparation (FASP ...
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bioRxiv - Molecular Biology 2021Quote: ... and incubated overnight at 25 °C or endoproteinase GluC in a GluC/protein ratio of 1/20 (w/w) (Promega Cat. No.V1651) and incubated overnight at 37 °C ...
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bioRxiv - Developmental Biology 2022Quote: ... protein in the gel pieces was digested using 20 ng/μl of Trypsin solution made in 25mM ammonium bicarbonate (Promega, Madison, WI) by incubating at 37°C overnight ...
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bioRxiv - Plant Biology 2022Quote: ... The pCMX-PL2-NterHA/MED14 vector was constructed as described above and expressed as HA-MED14 proteins using the TNT T7 Coupled Reticulocyte Lysate System (Promega, Madison, WI). HA-MED14 prey proteins were incubated with the affinity-purified GST-PIF4 or GST-HMR bait proteins immobilized on glutathione Sepharose 4B beads (GE Healthcare ...
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bioRxiv - Plant Biology 2022Quote: ... Protein complexes were then expressed in vitro using the TNT SP6 Coupled Wheat Germ Extract System (Promega, Madison, WI, USA, Cat #L4130) and purified using Magne HaloTag Beads (Cat #G7282) ...
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bioRxiv - Physiology 2022Quote: ... protein samples were digested by incubated overnight at 37°C with sequencing-grade modified trypsin (1/50, w/w; Promega, Madison, Wisconsin). Samples were acidified using 5% TFA and peptides cleaned up using the Phoenix 96× kit (PreOmics ...
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bioRxiv - Neuroscience 2022Quote: ... Protein digestion was processed for overnight by adding LysC and trypsin in a 1:50 ratio (µg of the enzyme to µg of protein; Promega; Cat#: V5071)) at 37° C and 1400 rpm ...
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bioRxiv - Systems Biology 2022Quote: ... 102.2 µL of 100 mM TEAB was added and proteins were digested for 16 hours at 37°C using 5 µg of trypsin (dissolved in trypsin resuspension buffer, Promega, Walldorf, Germany). Tryptic digestion was stopped by addition of 2.5 µL 100% formic acid ...
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bioRxiv - Developmental Biology 2022Quote: ... the expression of the fusion protein was initiated by washing out Dox and changing the media into CDMi containing protected furimazine (Promega; 1 μM). After the NLuc signal was confirmed 5-8 hours later ...
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bioRxiv - Systems Biology 2023Quote: ... proteins were precipitated with isopropanol50 and digested overnight at 37°C with 1:50 enzyme : protein ratio by Trypsin/Lys-C Mix (Promega GmbH, Germany). Resulting peptides were desalted on MicroSpin column (The Nest Group ...
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bioRxiv - Cancer Biology 2023Quote: T cell cytotoxicity induced by BiTEs and BiTE-sialidase fusion proteins was measured by lactate dehydrogenase (LDH) release using CytoTox 96® Non-Radioactive Cytotoxicity Assay (Promega, G1780). 10000 tumor cells and 50000 hPBMCs per well in 100 μL media were exposed to different treatments and incubated in 96 well plates at 37 °C (unless different ratio was specified elsewhere) ...
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bioRxiv - Cancer Biology 2023Quote: ... The protein solution was spun onto an S-Trap device (Protifi, Farmingdale, NY) and digested by 750 ng trypsin (Promega, Madison, WI) in 100 mM TEAB buffer at 37°C overnight ...
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bioRxiv - Systems Biology 2023Quote: ... and the bound proteins were subjected to on-cartridge digestion with mass spec grade Trypsin/Lys-C Rapid digestion enzyme (Promega, Madison, WI) at 70°C for 2h ...
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bioRxiv - Cancer Biology 2023Quote: ... and the protein pellet was resuspended in 50 mM ammonium bicarbonate and digested with sequencing-grade modified trypsin (Promega, Madison, WI, USA) overnight at 37°C ...
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bioRxiv - Neuroscience 2024Quote: ... or shRNA constructs with viral enzymes and envelope proteins (pMDLg/RRE, pRSV-REV and pVSV-G) using FuGENE6 transfection reagent (Promega Cat# E2692). HEK293T media was replaced with Neurobasal media (Thermo Fisher Scientific Cat# 21103049 ...
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bioRxiv - Synthetic Biology 2021Quote: All Plasmids were purified using the Midiprep kit (QIAGEN or Promega)
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bioRxiv - Cancer Biology 2021Quote: Dual-luciferase report assay was determined with the kit (Promega, E1910) following manufacturer’s protocols ...
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bioRxiv - Immunology 2021Quote: ... The luciferase activity were measured using a luciferase assay kit (Promega) and subtracted with the background level obtained from co-cultures in the absence of mAb.
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bioRxiv - Cell Biology 2020Quote: ... used for the Luciferase reporter assay (Steady Glow Luciferase Kit, Promega) and quantified as Relative Light Unit (RLU ...
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bioRxiv - Cell Biology 2020Quote: ... Extracts were analyzed using the Dual-Luciferase Reporter Assay Kit (Promega). The ratio of Luciferase/Renilla was calculated and normalized to experimental control (promoter in absence of experimental plasmid).
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bioRxiv - Cell Biology 2020Quote: Luciferase assays were performed with the dual luciferase assay kit (Promega). Cells were lysed using passive lysis buffer ...
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bioRxiv - Cell Biology 2020Quote: ... Luciferase expression levels were analyzed using the Dual Luciferase kit (Promega) in a DTX880 Multimode detector (Beckman Coulter).
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bioRxiv - Immunology 2021Quote: ... cDNA was synthesized with the M-MLV reverse-transcriptase kit (Promega) and diluted 1 in 5 before further application ...
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bioRxiv - Genetics 2021Quote: ... PCR were performed with the kit GoTaq® Flexi (Promega, USA) using 20 ng DNA ...
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bioRxiv - Neuroscience 2020Quote: ... and transcribing using the T7 RiboMAX RNA Production kit (Promega-P1320). Cas9 was transcribed from the plasmid pCS2-Cas9 (Addgene ...
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bioRxiv - Genomics 2021Quote: ... and Maxwell® RSC ccfDNA Plasma Kit (Promega, Leiden, the Netherlands). DNA was eluted in elution buffers provided by the kits used (QIAamp kit ...
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bioRxiv - Microbiology 2019Quote: ... DNA extraction was performed using the Maxwell Cell tissue kit (Promega). The primers ...
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TSG101 Associates with PARP1 and is Essential for PARylation and DNA Damage-induced NF-κB ActivationbioRxiv - Molecular Biology 2021Quote: Cell viability was assayed using the CellTiter-FluorTM kit (Promega, G6081) according to manufacturer’s instructions ...
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bioRxiv - Evolutionary Biology 2021Quote: ... This was purified using a PCR clean up kit (Promega, A9281). The sgRNAs were synthesized using the MEGAscript™ T7 Transcription Kit (Invitrogen ...
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bioRxiv - Microbiology 2022Quote: ... Luciferase activity was measured by Luciferase Assay kit (Promega, WI, USA). The 50% neutralization titer (NT50 ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... The CellTiter-Glo® Luminescent Cell Viability Assay Kit (Promega, # G7570) was used as recommended in the manufacturer’s protocol ...
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bioRxiv - Biochemistry 2022Quote: ... with an adenosine triphosphate (ATP) content assay kit (catalog 6016949, Promega).
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bioRxiv - Molecular Biology 2020Quote: ... total RNA was reverse-transcribed using GoScript Reverse Transcriptase Kit (Promega). Second ...
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bioRxiv - Cell Biology 2022Quote: ... A549 cells were solubilized using the Dual-Luciferase Assay Kit (Promega), and luciferase activity was quantified using a microplate dual-injector luminometer (Veritas ...
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bioRxiv - Immunology 2022Quote: ... cDNA was then synthesized using the reverse transcription kit from Promega. Transcriptional levels of PD-L1 (Forward ...
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bioRxiv - Immunology 2021Quote: ... CytoTox 96 LDH-release assay kit (G1780) was purchased from Promega.
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bioRxiv - Cell Biology 2020Quote: ... The dual luciferase reporter assay kit was purchased from Promega (E1910). Dulbecco’s Modified Eagle’s Medium (DMEM) ...
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bioRxiv - Microbiology 2019Quote: ... gDNA was isolated using the Wizard Genomic DNA extraction kit (Promega). gDNA was then fragmented to 400-600 bp by sonication (Covaris E220 ...
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bioRxiv - Microbiology 2020Quote: ... and Gaussia activity was measured using a Renilla luciferase kit (Promega) on a FLUOstar Omega plate reader (BMG Labtech) ...
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bioRxiv - Biochemistry 2019Quote: ... Luciferase assays were performed using a dual-luciferase assay kit (Promega), quantified with monolight 3010 luminometer (BD Biosciences) ...
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bioRxiv - Biophysics 2019Quote: ... cDNA was transcribed from RNA (Reverse Transcriptase kit, Promega, Madison, USA) using Reverse Transcriptase (Roche ...
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bioRxiv - Cancer Biology 2019Quote: Cellular glutathione was quantified using the GSH/GSSG-Glo kit (Promega) according to the instructions provided by the manufacturer ...
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bioRxiv - Genetics 2019Quote: ... and cDNA synthesis (M-MLV RT kit, Promega Corporation, cat. #M170A) were performed according to manufacturer’s specifications [21] ...
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bioRxiv - Evolutionary Biology 2019Quote: ... DNA was extracted using the Wizard Genomic DNA purification kit (Promega). Data on genome-wide admixture ...
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bioRxiv - Physiology 2019Quote: The CellTiter-Glo luminescent cell viability assay kit (Promega, Madison, WI) was used to determine the viability of primary hepatocytes ...
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bioRxiv - Cell Biology 2019Quote: ... For small scale validation experiments the Luciferase Assay System kit (Promega) was used as directed by manufacturers instructions and luminosity measured a using Lumat LB 9507 luminometer (Berthold Technologies).
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bioRxiv - Neuroscience 2020Quote: ... Reverse transcription was performed with an RT cDNA Synthesis Kit (Promega). Real-time PCR was performed with SYBR green SuperMix using an iCycler real-time PCR detection system (Bio-Rad).
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bioRxiv - Cancer Biology 2021Quote: ... Luminescence was obtained after applying the Dual-Luciferase Assay Kit (Promega) in a 96-well opaque plate following manufacturer’s protocol and measured on Perkin Elmer Victor X3 Plate Reader ...
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bioRxiv - Bioengineering 2021Quote: ... The LDH kit (CytoTox 96® non-radioactive cytotoxicity assay, Promega) was used and LDH assay was performed according to manufacturer’s instructions in which the number of cytotoxic cells is quantified by measuring cytosolic LDH enzyme leakage into the culture medium as a result of cell membrane damage [37] ...