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Citations for Promega :
1551 - 1600 of 2119 citations for Porcine Circovirus 2 PCV2 Protein since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2021Quote: ... The sample was diluted 1:7 with 0.1 M NH4HCO3 before overnight digestion of proteins with trypsin (Promega, cat#V5113) at 30°C ...
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bioRxiv - Cell Biology 2021Quote: ... v/v) and dehydration in ACN, proteins were digested overnight at 37 °C with trypsin (1:50, w/w) (V5280, Promega). Peptides were extracted from the gel in 50% ACN/0.1% formic acid ...
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bioRxiv - Molecular Biology 2021Quote: ... Samples were digested either with trypsin in a trypsin/protein ratio of 1/50 (w/w) (Promega Cat. No. V5111) and incubated overnight at 37 °C ...
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bioRxiv - Biochemistry 2021Quote: Urinary proteins (100 μg of each sample) were digested with trypsin (Trypsin Gold, Mass Spec Grade, Promega, Fitchburg, WI, USA) using filter-aided sample preparation (FASP ...
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bioRxiv - Molecular Biology 2021Quote: ... Proteins on the filter unit were digested with 40 μl of trypsin solution (trypsin [V5111, Promega] in 50 mM NH4HCO3) at 37°C for 18 h ...
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Intrarenal B cells integrate in situ innate and adaptive immunity in human renal allograft rejectionbioRxiv - Immunology 2020Quote: ... Trypsin digestion was carried out overnight at 37 °C with 1:50 - 1:100 enzyme-protein ratio of sequencing grade-modified trypsin (Promega) in 50 mM NH4HCO3 pH 7.5 ...
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bioRxiv - Cell Biology 2021Quote: ... Eluted protein was labeled with either 1.75 µM AlexaFluor 660 or 2.5 µM TMR HaloTag Ligands (Promega, G8471 and G8251) for 2 hr on ice ...
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bioRxiv - Cell Biology 2023Quote: ... 1.5 μg of total DNA (1:10 ratio of the Cre plasmid to the fusion protein plasmid) were mixed with 6 μl of Fugene 6 transfection reagent (Promega) in 100 μl of Opti-MEM ...
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bioRxiv - Cell Biology 2023Quote: ... packed column (now loaded with the protein sample) 20 µL of Trypsin digestion solution (1 µg MS-grade Trypsin (Promega) in 50 mM ammonium bicarbonate ...
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bioRxiv - Molecular Biology 2023Quote: ... was removed by evaporation using speed-vac and the residue was used to produce L-[2,3,4,5-3H]-proline labeled procollagen chains and the collagen-like proteins in the TnT® Coupled Reticulocyte Lysate Systems (Promega) using either SP6 or T7 promoters ...
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bioRxiv - Plant Biology 2023Quote: Myc- and FLAG-tagged versions of the different ALOG proteins were produced using the Quick Coupled Transcription/Translation System (TnT; Promega). 25 µL of TnT reactions producing indicated proteins were mixed with Buffer 1 to reach 150 µL and rotated at 4 °C during 1 h ...
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bioRxiv - Plant Biology 2023Quote: ... Protein yield was quantified by measuring luminescence with the Nano-Glo® HiBiT Extracellular Detection System (N2420, Promega, Madison, WI) and HiBiT Control Protein (N3010 ...
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bioRxiv - Molecular Biology 2022Quote: ... Expression of Halo-bZIP and SBP-bZIP proteins were confirmed by western blotting using anti-HaloTag monoclonal antibody (Promega, G9211) and anti-SBPTag mouse anti-human antibody (MilliporeSigma ...
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bioRxiv - Cancer Biology 2023Quote: ... Alkylated proteins were then cleaved using a two-step digestion: first with Endoproteinase Lys-C (ratio 1:33 enzyme: lysate, Promega) for 1 hour at 37 °C then with Trypsin (ratio 1:33 enzyme ...
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bioRxiv - Biochemistry 2024Quote: ... at a 1:75 enzyme to protein ratio for 6 h at 30 °C and then by trypsin (V5111, Promega) (1:50 enzyme to protein ratio ...
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bioRxiv - Cancer Biology 2024Quote: ... Denatured proteins were enzymatically digested for 14 h at 37 °C with 1 µg of sequencing-grade Trypsin (V511A, Promega). After speed-vaccum drying ...
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bioRxiv - Developmental Biology 2024Quote: ... Total peptide concentration was adjusted to 0.15 µg/µL according to the absorbance at 280 nm against a Mass Spec-Compatible Human Protein Extract/Digest calibration curve (Promega), using the Thermo Nanodrop 1000 ND-1000 spectrophotometer (Thermo Fisher).
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bioRxiv - Microbiology 2024Quote: ... difficile was mixed with 50 µL kit reagents (100-fold diluted LgBiT protein, 50-fold diluted substrate in kit buffer, Promega). Subsequently ...
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bioRxiv - Plant Biology 2023Quote: ... Proteins were produced using 5 µg of plasmids in 25 µL in vitro translation reaction of TnT SP6 High-Yield Wheat Germ Protein Expression System (Promega). For the OSH15-RI complex ...
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bioRxiv - Plant Biology 2023Quote: ... 2D gels were stained by Coomassie brilliant blue and proteins were digested in-gel using trypsin (modified, sequencing purity, Promega), as previously described (Casabona et al. ...
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bioRxiv - Cancer Biology 2024Quote: ... the protein precipitate was air-dried and resuspended in 200ul of 25mM ammonium bicarbonate solution and 3ul of trypsin (Promega) and digested overnight at 37°C ...
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bioRxiv - Microbiology 2023Quote: ... VSV-G agarose beads and bound proteins were then treated with 10 μL of 10 μg/μL sequence grade trypsin (Promega) for 16 h at 37℃ with mild shaking ...
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bioRxiv - Biochemistry 2023Quote: ... N- glycan release was achieved after digestion with PNGase F (1 U/10 µg protein at 37 °C overnight; Promega). Released N-glycans were hydrolysed with 25 µl of 100 mM ammonium acetate at pH 5 (1 h at RT) ...
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bioRxiv - Biochemistry 2023Quote: ... The expression of FgSte3-NLuc donor protein produced was also validated performing immunoblotting using anti-Nluc antibody (Promega cat#7000), see details below.
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bioRxiv - Developmental Biology 2022Quote: ... Proteins were on-bead digested using 5 μl of Sequencing Grade Trypsin (100 ng/μl in 10 mM HCl, Promega). The digestion was carried out in a microwave instrument (Discover System ...
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bioRxiv - Biochemistry 2022Quote: ... The proteins were rinsed with urea and ammonium bicarbonate solutions and digested overnight at 37°C using trypsin gold (Promega) at a ratio of 1:100 ...
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bioRxiv - Biochemistry 2022Quote: ... The 90% buffer was removed and protoplasts were flash-frozen in liquid nitrogen to quench the protein degradation process followed by luciferase activity assay (E1500, Promega) to determine the relative amount of RAP2.12(1-28)-luciferase chimera protein remaining at various time points ...
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bioRxiv - Developmental Biology 2022Quote: A total of 100 μg of protein was digested with trypsin (Trypsin Gold, Mass Spec Grade, Promega, Fitchburg, WI, USA) using filter-aided sample preparation (FASP ...
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bioRxiv - Microbiology 2022Quote: Cell viability of different 2D cultures at different time points of infection or exposure to virus proteins or HAE supernatant was determined using the CellTiter 96 AQueous One Solution cell proliferation assay (G3582; Promega), while HTO viability was determined by the CellTiter-Glo 3D cell viability assay (Promega G9681 ...
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bioRxiv - Molecular Biology 2022Quote: ... a total of 225 μg of protein extract isolated using the aforementioned hypotonic buffer (additionally supplemented with 100 U/ml RNasin Plus RNase Inhibitor (Promega) and 2 U/mL Turbo DNase (Ambion) ...
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bioRxiv - Plant Biology 2022Quote: ... were reduced with DTT and then alkylated with iodoacetamide and digested overnight using Lys-C/Trypsin (1:50, enzyme to protein; Promega). After terminating the digestion with 1% trifluoroacetic acid ...
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bioRxiv - Microbiology 2023Quote: ... the samples were digested by trypsin with 1/80 (w/w) trypsin/total protein ratio (Sequencing Grade Modified Trypsin, Promega) according to the manufacturer recommendations ...
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bioRxiv - Cancer Biology 2023Quote: ... before drying further with a speed vac until the pieces were ‘dice-like bouncy.’ The protein samples were then incubated in digestion buffer [50 mM NH4HCO3 and 10 μg/mL Sequencing grade modified trypsin (Promega)] overnight at 37°C ...
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bioRxiv - Biophysics 2023Quote: 5 μM purified EGFP-YbbR-Lphn3 GAIN-HaloTag protein in HBS was mixed with 5.2 μM HaloTag PEG-Biotin Ligand (Promega, G8592) and incubated at room temperature for 2 hours to biotinylate the CTF of the GAIN construct ...
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bioRxiv - Cell Biology 2022Quote: Halo-3F-SOCS1 expression was induced with doxycycline overnight (1 μg/mL) and Halo-tagged protein detected by incubation with fluorescent Halo TMR ligand (10 nM; Promega) overnight ...
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bioRxiv - Developmental Biology 2023Quote: ... The modified proteins were digested with lysyl endopeptidase (Lys-C) (Wako) for 3h at 37° C and subsequently digested with trypsin (Promega) overnight at 37° C ...
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bioRxiv - Molecular Biology 2023Quote: ... Strap binding buffer was applied to precipitate proteins on quartz and proteolysis took place during 14 hrs at 37°C with 1 µg Trypsin sequencing grade (Promega). After speed-vacuum drying of eluted peptides ...
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bioRxiv - Microbiology 2023Quote: 1 ml of recombinant GST or GST-YgfB protein at a concentration of 10 μM was incubated with 100 μl 50 % MagneGST (Promega) bead-slurry equilibrated with pulldown-buffer for 45 min at 4°C and washed two times with 500 μl pulldown-buffer ...
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bioRxiv - Immunology 2023Quote: ... Protein expression in luciferase-based assays was verified by analyzing the corresponding lysates stored in 1x passive lysis buffer (Promega) by Tricine-SDS-PAGE ...
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bioRxiv - Plant Biology 2023Quote: ... Transcription factors were expressed using at least 2000 ng PCR product per sample with the TnT T7 Quick for PCR DNA in vitro protein expression kit (Promega). All reaction volumes were doubled to yield a total of 100 µL protein product per transcription factor ...
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bioRxiv - Genetics 2023Quote: ... The HALO-NAC61 and GST-HALO (used as negative control) fusion proteins were translated in vitro using the TNTR SP6 coupled reticulocyte lysate system (Promega). Two replicates were used for the TF and input libraries ...
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bioRxiv - Developmental Biology 2024Quote: Substrate titration kinase reactions were performed on the indicated recombinant LIN-42 proteins (CK1BD + Tail WT or mutants) using the ADP-Glo kinase assay kit (Promega) according to the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2024Quote: ... The expression of the NLuc-fusion proteins was initiated by washing out Dox and changing the medium into CDMi containing protected furimazine (Promega). After the NLuc signal was confirmed 4-5 hours later ...
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bioRxiv - Plant Biology 2024Quote: ... and 1ug of plasmid DNA was used for in vitro protein expression using the TNT rabbit reticulocyte expression system (Promega) per the manufacturer’s instructions ...
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bioRxiv - Microbiology 2024Quote: ... 50 μL sample containing 50 μg proteins were mixed with 150 μL of rapid digestion buffer (Promega, Madison, Wisconsin, USA). Rapid trypsin (Promega ...
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bioRxiv - Neuroscience 2024Quote: The HaloTag-fused proteins expressed in neurons (10 DIV) placed on coverslips were fluorescently labeled with 1 µM AF660 (Promega) in medium for 15 min at 37°C ...
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bioRxiv - Microbiology 2024Quote: Enzymatic deglycosylation of denatured protein samples was performed with PNGase F (1 Unit/μL) obtained from Promega (Madison, WI, USA). First ...
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bioRxiv - Immunology 2024Quote: ... 2 µg of anti-DNA-PKcs antibody or the corresponding IgG control was incubated with 70 ng of recombinant DNA-PKcs protein (Promega) in 500 µL of lysis buffer (20 mM Tris–HCl [pH 7.4] ...
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bioRxiv - Immunology 2024Quote: ... or 0.05 μg proteins expression plasmids or empty pAc5.1A plasmids (as controls) using the Fugene HD Transfection Reagent (Promega Shanghai, China) according to the user manual ...
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bioRxiv - Molecular Biology 2024Quote: Halo-tagged fusion proteins (200 pmol) were bound to 10 μL of Magne® HaloTag® Beads (20% slurry, Promega), followed by washing ...