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Citations for Promega :
1551 - 1600 of 1948 citations for Dog Trefoil Factor 3 TFF3 Protein since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Systems Biology 2023Quote: ... then proteins were digested with 1 μg of mass spectrometry grade Trypsin/Lys-C enzyme mix (Promega) in 50 μL of digestion buffer (100 mM ABC ...
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bioRxiv - Plant Biology 2023Quote: The total proteins (250 μg for each sample) were digested by FASP procedure using trypsin (Promega Corporation) as described previously60 ...
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bioRxiv - Cell Biology 2023Quote: ... CTPS activity of purified CTPS1 and CTPS2 proteins was then analyzed using ADP Glo Kinase assay (Promega). CTPS1 and CTPS2 were diluted in assay buffer (50 mM Trizma ...
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bioRxiv - Microbiology 2023Quote: ... Proteins were digested overnight (21 h) at 37 °C with 0.1 μg/μL trypsin (sequencing grade, Promega) dissolved in 1 mM HCl ...
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bioRxiv - Plant Biology 2023Quote: ... Protein marker was purchased from Fermentas (Beijing) and M-MLV reverse transcriptase was purchased from Promega (Beijing). Homologous recombinant kit was purchased from Clone Smarter (Beijing) ...
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bioRxiv - Biochemistry 2023Quote: ... The performance of the LC-MS system was controlled by analyzing a yeast protein extract digest (Promega). A total of eight datasets were collected and subsequently processed using three de novo sequencing algorithms CasaNova ...
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bioRxiv - Microbiology 2023Quote: ... Proteins were digested overnight at room temperature with 0.4 µg of sequencing grade modified trypsin (Promega, #V5111) in 40 µl Hepes/NaOH ...
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bioRxiv - Bioengineering 2024Quote: ... Protein (25 µg per sample) was digested overnight at 37 °C with trypsin endoproteinase (Trypsin Gold, Promega), and the digested peptides were desalted using 1 cc C18 cartridges (Waters) ...
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bioRxiv - Cell Biology 2024Quote: ... IMR90 exosome proteins trapped in the S-Trap columns were digested with trypsin (Promega, Madison, WI, # V5111) in digestion buffer (50 mM TEAB ...
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bioRxiv - Plant Biology 2024Quote: Phosphorylation of HUB2 by SnRK2s was examined with ADP-Glo Protein Kinase Activity Assay Kit (Promega, V9101) with slight modifications (Kathania et al. ...
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bioRxiv - Cell Biology 2024Quote: ... Proteins on the beads were digested by adding 200 ng of a trypsin/Lys-C mix (Promega) at 37°C overnight ...
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bioRxiv - Developmental Biology 2024Quote: Abd-B proteins for EMSA were produced using the TNT coupled in vitro transcription/translation system (Promega). Protein production was estimated by labeling the proteins with 35S-methionine and found to be produced at similar amounts ...
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bioRxiv - Microbiology 2024Quote: ... Protein digestion occurred overnight at 37 °C and 300 rpm with 1.5 µg sequencing grade trypsin (Promega). 0.5 pmol of the PierceTM Peptide Retention Time Calibration mix (Thermo Scientific ...
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bioRxiv - Molecular Biology 2024Quote: ... Proteins were digested overnight at 37°C with 2 µg trypsin (Trypsin Gold, Mass spectrometry Grade, Promega). Digestion was stopped by adding 70 µl 1% trifluoroacetic acid (TFA ...
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bioRxiv - Systems Biology 2024Quote: ... Protein digestion was carried out overnight at 37°C with sequencing grade trypsin (Promega, Madison, WI, USA) at 1:40 (w/w ...
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bioRxiv - Cell Biology 2024Quote: ... Proteins were digested in-gel overnight at 37°C with Sequencing Grade Modified Trypsin (Promega; cat. #V5111) diluted in 200uL of digestion buffer (40 mM ammonium bicarbonate ...
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bioRxiv - Plant Biology 2020Quote: ... corniculatus plants was synthesized from 3 μg of total RNA using a Moloney Murine Leukemia Virus Reverse Transcriptase (MMLV-RT) (Promega, WI, USA) and 100 pmol of random hexamers (Pharmacia Biotech) ...
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bioRxiv - Cancer Biology 2021Quote: ... DNA:FuGENE complexes were formed at a ratio of 1:3 (μg DNA/μL FuGENE HD) according to the manufacturer’s protocol (Promega, Madison, WI, USA). The resulting transfection complex (1 part ...
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bioRxiv - Genomics 2021Quote: ... Cells were then grown for 3 days at which point the viability was measured using a CellTiter-Glo assay (Promega Cat# G9241) read out on a standard luminometer ...
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bioRxiv - Biochemistry 2020Quote: ... or PACAP (10 nM final) and assayed 3 hours later with the Nano-Glo® Dual-Luciferase® Reporter System (Cat# N1630; Promega) on a Berthold Sirius luminometer to quantify ERK activation.
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bioRxiv - Biophysics 2020Quote: ... P1KO cells were transiently transfected in a 6-well plate (the cells were seeded 8 h before transfection) in the presence of ruthenium red (10 μM) and mouse Piezo1 (3 μg) using Fugene6 (Promega, Madison, WI). 20–30% of cells showed positive GFP expression indicating successful Piezo1 transfection.
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bioRxiv - Cell Biology 2021Quote: ... Primary antibodies were incubated in PBDS for 3 days at 4°C followed by 4 washes over the next 24 hours with 0.2% Tween-20 (Promega UK Ltd; H5151) in PBS ...
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bioRxiv - Bioengineering 2022Quote: ... The DNA transfection reagents were prepared by incubating 3 µg of plasmid DNA encoding the Bxb1 recombinase and 6 µL of Fugene6 reagent (Promega Cat # E2693) in 300 µL of Opti-MEM for 15 minutes and then added to the cell suspension ...
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bioRxiv - Biochemistry 2022Quote: The 4330bp PCR product amplified from M13mp18 by using primers: oM13-5-27 and oM13-3-24 was inserted into pGEM®-T Easy(PROMEGA #A137A). The resultant plasmid is named as pM13 ...
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bioRxiv - Microbiology 2022Quote: ... PAO1-WT and few colonies that grew in 3 mM 22D-chelated condition were isolated and gDNA extracted using Wizard® Genomic DNA Purification Kit (Promega, USA) for use in whole genome sequencing ...
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bioRxiv - Cell Biology 2022Quote: ... anti-goat alkaline phosphatase-linked antibody and alkaline phosphatase substrate (5-bromo-4-choloro-3-indolyl 1-phosphate and nitroblue tetrazolium) from Promega (Southampton, UK); a hydroxamate-based MMP inhibitor CT-1746 (N1-[2-(S)-(3,3-dimethylbutanamidyl)]-N4-hydroxy-2-(R)-[3-(4-chlorophenyl)-propyl]-succinamide ...
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bioRxiv - Neuroscience 2022Quote: ... and mouse cholecystokinin (CCK, 3-688 of NM_031161) were PCR-amplified and subcloned into the pGEM-T Easy vector (Promega, Fitchburg, WI, USA). Digoxigenin (DIG)-labeled anti-sense probes were prepared using DIG RNA Labeling Mix (Sigma ...
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bioRxiv - Molecular Biology 2020Quote: ... The NanoLuc and Fluc luciferase activities were measured 3 days after transfection using the Nano-Glo® Dual-Luciferase® Reporter Assay System (Promega). The firefly (Fluc ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... RBD-R: 5′-TGC CTC GAG CTC AAG TGT CTG TGGATC AC-3′) into pGEM T-easy vector (Promega, Madison, WI, USA). A standard curve was generated by determining the copy numbers derived from serial dilutions of the plasmid (103–109 copies) ...
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bioRxiv - Microbiology 2020Quote: ... 3’-untranslated region (3’-UTR) fragment of IκBα (human/rat) was inserted into the pGL3-basic vector (firefly luciferase; Promega, Madison, WI, USA), which was obtained from General Biosystems (Anhui ...
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bioRxiv - Cancer Biology 2022Quote: ... we followed the genotyping method for KrasG12D mutation previously described.3 Genomic DNA was isolated from formalin fixed paraffin embedded thymus slides using ReliaPrep FFPE gDNA MiniPrep System (Promega, Madison, WI). The DNA was then amplified using polymerase chain reaction (PCR ...
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bioRxiv - Cancer Biology 2022Quote: Apoptosis induction in response to SRRM1 silencing in leukemia cell lines (25,000 cells/well onto white-walled multiwell luminometer plates) was performed by using Caspase-Glo® 3/7 Assay (Promega Corporation, #G8091) as previously reported (67) ...
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bioRxiv - Biochemistry 2021Quote: The promoter fragment of luciferase reporter plasmid pGADD34-0.5k generated by Eurofins Japan (Tokyo, Japan) was subcloned into a pGL-3 vector (Promega, Madison, WI, USA) by restriction enzyme cutting site KpnI/HindIII ...
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bioRxiv - Molecular Biology 2021Quote: ... A total of 24 clones (4 holobionts x 3 replicates x 24 clones = three 96-well plates) from each amplicon were amplified directly with GoTaq polymerase (Promega, Lyon France) in a 25 μL reaction volume using the following PCR parameters ...
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bioRxiv - Cell Biology 2022Quote: ... Control and treated cells were cultured for 1 day in 96-well plates then treated with small molecules for 3 consecutive days before incubation with 120 μL fresh media containing 20 μL of CellTiter 96® AQueous One Solution (Promega, G3580) for 1 to 4 hours at 37°C in a humidified ...
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bioRxiv - Molecular Biology 2022Quote: ... SEA (0.2 mg/ml) and soluble schistosomula antigens (0.2 mg/ml) was determined using the Caspase-Glo® 3/7 Assay System (Promega, Sydney, Australia) according to the manufacturers’ instructions.
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bioRxiv - Neuroscience 2023Quote: ... Cells were transfected with 1 µg of DNA diluted in 45 µl dMEM and then mixed with 3 µl ViaFect™ (Promega, USA) transfection reagent added and incubate for 20 minutes ...
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bioRxiv - Cell Biology 2022Quote: ... approximately 200,000 HEK-293T cells were transfected with 1 μg of plasmid DNA complexed to 3 μL of FuGENE HD (Promega, Cat: E2311). 16-20 hours post transfection the cells were dissociated from the plastic using cell dissociation buffer (Gibco Cat ...
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bioRxiv - Bioengineering 2023Quote: ... The effect on cell viability of PTNP was assessed using the MTS [(3-(4,5-dimethylthiazol-2-yl)-5-(3- carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium)] assay (CellTiter 96 cell proliferation assay kit; Promega, WI, USA) (92) ...
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bioRxiv - Microbiology 2023Quote: The quantification of viral RNA was carried out in one-step RT-qPCR using 3 µl of the eluted RNA with the GoTaq® Probe One-step RT-qPCR System (Promega, Poland). This procedure employed in-house primers and a probe specifically tailored for the HCoV-229E N sequence ...
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bioRxiv - Evolutionary Biology 2023Quote: The 419 bp sequence of 3’UTR containing predicted target sites for miR-252 in CcTRPM was cloned into the pmirGLO vector (Promega, Wisconsin, USA) downstream of the luciferase gene to construct the recombinant plasmid of CcTRPM-3’UTR-pmirGLO using the pEASY®-Basic Seamless Cloning and Assembly Kit ...
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bioRxiv - Cancer Biology 2024Quote: ... tissues were incubated with primary antibodies diluted in 10% DS in PB1 for 3 days at 4°C followed by four washes over the following day with 0.2% Tween-20 (Promega UK Ltd, H5151) in PBS ...
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bioRxiv - Molecular Biology 2024Quote: ... 16.5 μg attB-plasmid library + 3 μg pCAG-NLS-Bxb1 + 2 μg pMAX-GFP + 2150 μL serum-free DMEM + 80 μL FugeneHD (Promega cat# E2311), then incubated at room temperature for 15 min and added dropwise to cells ...
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bioRxiv - Plant Biology 2024Quote: ... The transfected protoplasts were aliquoted into a 96-well plate with 200 nM D-luciferin/well with 3 biological replicates and then measured with a luminometer (Promega Max NAVIGATOR) every hour for 16 h.
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bioRxiv - Developmental Biology 2024Quote: ... tRFValCAC sequence was inserted into the multiple-cloning site at the 3’ end of the hRluc gene within the psiCHECK-2 Vector (Promega, cat # C8021). mESCs were transfected using the method described above ...
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bioRxiv - Cancer Biology 2024Quote: Caspase-3/7 and -8 activities were assayed using Caspase-Glo®- 3/7 and -8 assays according to manufacturer’s instructions (Promega, Madison, WI).
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bioRxiv - Cell Biology 2024Quote: ... the membranes were developed with a commercial solution of 5-bromo-4-chloro-3-indolyl phosphate (BCIP) and nitro blue tetrazolium (NBT) according to the manufacturer (Promega, Madison, WI), in alkaline developing solution ...
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bioRxiv - Molecular Biology 2021Quote: ... Reduced and alkylated gel pieces were processed for in-gel protein digestion by trypsin (Promega, Madison WI, USA) overnight at 37 °C adding ammonium bicarbonate buffer to cover gel matrix ...
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Multimodal tubulin binding by the yeast kinesin-8, Kip3, underlies its motility and depolymerizationbioRxiv - Biophysics 2021Quote: ... The protein was fluorescently labeled with 10uM TMR (dimer constructs) or 30uM TMR (monomer constructs, HALO-TMR; Promega) on beads for 1h at 4°C ...
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bioRxiv - Cell Biology 2021Quote: ... the bead-bound protein was incubated with either 6.7 μM HaloTag-TMR or HaloTag-PEG-biotin ligand (Promega) for 15 minutes at room temperature ...