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Citations for Promega :
1501 - 1550 of 3110 citations for Recombinant Human High Mobility Group Box 1 His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2024Quote: ... cells were lysed with 100 μL of 1 x PLB (Promega). Luciferase activities were measured by Dual-Luciferase Reporter Assay System (Promega) ...
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bioRxiv - Molecular Biology 2024Quote: ... anti-mouse IgG HRP conjugate (Promega, catalog no. W4021, 1:40,000), goat anti-rabbit IgG HRP conjugate (Cell Signaling Technology ...
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bioRxiv - Molecular Biology 2024Quote: ... anti-mouse IgG (H+L) HRP conjugate (Promega W4021, 1:2,500) and anti-rat IgG (H+L ...
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bioRxiv - Molecular Biology 2024Quote: ... anti-mouse IgG HRP conjugate (Promega, catalog no. W4021, 1:40,000). Unless otherwise stated ...
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bioRxiv - Biochemistry 2024Quote: ... with on-column RQ1 RNase-Free DNase 1 treatment (Promega, USA). cDNA was synthesized using the SuperScriptTM IV First-Strand Synthesis System (ThermoFisher ...
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bioRxiv - Plant Biology 2024Quote: ... anti-goat IgG conjugated to HRP (1:10,000, Promega, PA1 28664), anti-H3 (1:2500 ...
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bioRxiv - Physiology 2024Quote: ... Overnight digestion with Lys-C/Trypsin combo (1:50 enzyme:protein; Promega). After stopping the digestion with 1% trifluoroacetic acid ...
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bioRxiv - Molecular Biology 2020Quote: ... Prior to the addition of 1 μl of MMLV-RT enzyme (Promega), the reaction was incubated at 65°C in order to denature double stranded RNA ...
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bioRxiv - Cell Biology 2019Quote: ... Any potential contaminating DNA was removed by DNase digestion (RQ-1, Promega); RNA was subsequently purified on a column (RNA Clean & Concentrator-25 ...
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bioRxiv - Developmental Biology 2021Quote: ... The primary antibodies used are mouse β-Gal (1:250; Promega #Z3781), mouse anti-Wingless (1:100 ...
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bioRxiv - Cancer Biology 2022Quote: ... Histones were then digested with 1 μg of sequencing grade trypsin (Promega) diluted in 50mM ammonium bicarbonate (1:20 ...
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bioRxiv - Molecular Biology 2022Quote: ... in the presence of 15% PEG8000 and 1 U/μl RNasin (Promega) at 18°C overnight and then at room temperature for 1 hour and inactivated at 65°C for 20 min ...
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bioRxiv - Molecular Biology 2021Quote: ... 1 μL of 100× diluted furimazine (Nano-Glo luciferase assay reagent, Promega) was added to each well ...
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bioRxiv - Molecular Biology 2021Quote: ... transfected with 1 µg DNA and 3 µl FuGENE HD (Promega, E2311) in 150 µl Opti-MEM media according to manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2021Quote: ... 200 μL of rabbit polyclonal antibody against PLRV-CP (1:5000; Promega) was added to each well and incubated for 3 h at 37 °C ...
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bioRxiv - Molecular Biology 2019Quote: ... The denatured RNA was mixed with 1 μl of rRNasin® (Promega), 5 μl VRC ...
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bioRxiv - Pathology 2020Quote: ... respectively) containing β-galactosidase (1 mg/ml; Cat # V394A; Promega, Madison, WI). Samples were post fixed in formalin overnight and then stored in ethanol (70%).
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bioRxiv - Molecular Biology 2019Quote: ... 1 μL (50 ng) of pGEM®-T-Easy vector (Promega, USA), 1 μL of 10X ligase buffer ...
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bioRxiv - Genomics 2021Quote: ... polyclonal anti-mouse IgG-HRP raised in goat (W402B, 1:3,000; Promega) and polyclonal anti-rabbit IgG-HRP raised in goat (R1364HRP ...
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bioRxiv - Biochemistry 2020Quote: ... The genomic DNA contamination was removed with 1 µl of DNaseI (Promega) treatment for 60 minutes at 37°C ...
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bioRxiv - Neuroscience 2021Quote: ... Dual-Glo Stop & Glo Reagent diluted (1:100) in culture medium (Promega) was added to each well and the luminescence of the renilla luciferase was measured after 20 min incubation at RT ...
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bioRxiv - Immunology 2021Quote: ... 1 mM L-Glutamin and 250 μM Luziferin D (Promega, Madison, USA). Cells were incubated 12-16 hours in a humidified incubator at 37°C and 5% CO2 to adhere to the bottom of the plate ...
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bioRxiv - Cell Biology 2021Quote: ... all buffers were supplemented with RNAse inhibitor (RNasin, #N2111, Promega, 1:320.000). Cells were mounted in Prolong Diamond (Thermo Scientific ...
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bioRxiv - Cell Biology 2021Quote: ... Cells were lysed with 1× passive lysis buffer (Promega, Madison, WI, USA). TOPFlash/Renilla luciferase assays were performed using the dual-luciferase reporter assay kit (Promega ...
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bioRxiv - Immunology 2020Quote: ... Cells were then lysed with 25μL of 1×passive lysis buffer (Promega) for 30 min at room temperature ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... GoTaq® Probe 1-Step RT-qPCR System was purchased from Promega. SARS-CoV-2 (2019-nCoV ...
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bioRxiv - Plant Biology 2021Quote: ... digested by 1 µg sequence-grade modified trypsin (Promega, Madison, WI, USA) at 37 °C overnight ...
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bioRxiv - Microbiology 2020Quote: ... The standard curve was generated using GoTaq 1-Step RT-qPCR (Promega).
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bioRxiv - Biochemistry 2022Quote: ... the proteins were digested with 1 μg trypsin/Lys-C mix (Promega) at 37 °C overnight ...
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bioRxiv - Molecular Biology 2022Quote: ... and 1 μl of either RNasin RNase inhibitor (Promega, 40 U/μl) or 10 mg/ml RNase A (Roche ...
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bioRxiv - Synthetic Biology 2022Quote: ... 2 μL BSA (1 mg/mL) and 2 μL buffer H (Promega) in reaction volume of 20 μL ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... GoTaq® Probe 1-Step RT-qPCR System was purchased from Promega. SARS-CoV-2 (2019- nCoV ...
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bioRxiv - Cell Biology 2022Quote: ... Secondary antibodies conjugated to horseradish peroxidase (1:10,000, W401B and W402B, Promega) were applied in the same solution for one hour at room temperature before activity was detected (SuperSignal West Pico PLUS 34580 ...
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bioRxiv - Microbiology 2021Quote: ... the sample was digested with 1 µg of sequencing grade trypsin (Promega) for 6 hours at 37 °C ...
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bioRxiv - Microbiology 2021Quote: ... and pVSV-G (4:3:1 ratio) using calcium phosphate transfection (Promega) following the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2021Quote: ... 60°C for 1 minute) using Go-Taq qPCR Master Mix (Promega) on a QuantStudio™ 7 Flex Real Time PCR System (ThermoFisher Scientific) ...
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bioRxiv - Neuroscience 2020Quote: ... or anti-rabbit IgG pAb conjugated alkaline phosphatase (1:10,000, S3731, Promega) were used as secondary antibodies and visualized by detecting chemiluminescence (32209 ...
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bioRxiv - Neuroscience 2021Quote: ... Equivalent amounts of RNA (1 µg) were treated with DNase I (Promega) at 37°C for 30 minutes ...
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bioRxiv - Genomics 2022Quote: ... 1 mM CaCl2 and sequencing-grade modified porcine trypsin (Promega, Madison, WI) was added to all protein samples at a 1:50 (w/w ...
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bioRxiv - Microbiology 2022Quote: ... 1 complete protease inhibitor tablet EDTA-free and 5 µl DNaseI (Promega), lysed using a cell disruptor and the insoluble fraction removed by centrifugation (15,000 xg ...
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bioRxiv - Neuroscience 2022Quote: ... and anti-activated Caspase 3 (rabbit, Promega, catalog #G7481, 1:250, RRID:AB_430875), and appropriate secondary antibodies ...
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bioRxiv - Neuroscience 2022Quote: ... RNA (1 μg) from each sample was reverse transcribed for PCR (Promega). PCR was conducted using GoTaq DNA polymerase (Promega) ...
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bioRxiv - Genetics 2019Quote: ... 1 mM CaCl2 with 2 ug trypsin (Promega, Madison, WI, product V5111). Digest was stopped with formic acid ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... The Nano-Glo Luciferase Assay Substrate furimazine (2 µl ml-1, Promega) was added and the luminescence was measured on a luminometer (Mithras LB 940 Berthold Technologies plate reader ...
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bioRxiv - Synthetic Biology 2020Quote: ... was then digested with proteomics-grade trypsin (1:75 w/w; Promega) in 100 mM Tris-HCl ...
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bioRxiv - Genetics 2020Quote: ... in SHK-1 cells with Fugene HD transfection reagent (Promega, Madison, USA). To achieve this ...
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bioRxiv - Microbiology 2021Quote: ... 1 μg of total RNA with 0.5 μg of random primers (Promega) and distilled water up to 10 μl of final volume were incubated at 70°C for 5 minutes and then kept on ice for 5 minutes ...
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bioRxiv - Molecular Biology 2021Quote: ... 20 μl of 5 ng μl−1 trypsin (sequencing grade, Promega®) in 50 mM AHC plus 1 mM CaCl2 were added to each well and incubated for 20 min at 4°C ...
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bioRxiv - Molecular Biology 2021Quote: ... 250 ng each of the pHMRluc (1) and pGL3-control plasmids (Promega) were added in addition to the indicated amounts of SP expression constructs.
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bioRxiv - Molecular Biology 2020Quote: ... or miR-324-3p.1 were amplified and inserted into pmirGLO (Promega) at the XhoI and XbaI sites ...