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Citations for Promega :
1501 - 1550 of 3835 citations for 5 Nitro 1H benzo de isoquinoline 1 3 2H dione since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2021Quote: ... DNA was isolated from cell pellets and culture supernatants cleared of cell debris by centrifugation of 5 min at 14000 rpm and treated with 20 U/ml DNase I (Promega, Madison, WI, USA) to remove free viral DNA ...
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bioRxiv - Microbiology 2024Quote: ... Proteins were then solubilized in 50 mM ammonium bicarbonate for a reduction-alkylation step (dithiothreitol 5 mM – iodoacetamide 10 mM) and an overnight digestion with 300ng of sequencing-grade porcine trypsin (Promega, Fitchburg, MA, USA). Digested peptides were resuspended in 0.1% formic acid (solvent A ...
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bioRxiv - Cancer Biology 2022Quote: ... Immunoprecipitated protein–DNA complexes and 5% input were analyzed by qRT-PCR using GO taq QPCR master mix (Promega, A6002, lot no. 0000385100) in triplicate using primers specific for PFKFB3 HREs ...
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bioRxiv - Plant Biology 2022Quote: ... benthamiana leaf discs were ground to a fine powder in liquid nitrogen in 2 ml Eppendorf Safe-Lock tube (1 leaf disc in each tube) and subsequently incubated 5 min at room temperature (RT) with 200 µl of the Passive lysis buffer (Promega catalog number E1910). The resulting crude leaf extract was centrifuged at 20,000 g for 2 min at RT ...
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bioRxiv - Microbiology 2023Quote: ... Three μL (6 ng on average) of template DNA was added to 5 μL 5x GoTaq Green Reaction Mix Buffer (Promega, Madison, Wisconsin, USA), 0.625 units of GoTaq DNA Polymerase (Promega ...
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bioRxiv - Molecular Biology 2024Quote: ... 5 µl of each translation reaction was mixed with 5 µl 1x Renilla-Glo substrate in Renilla-Glo assay buffer and supplemented with 40 µl of H2O (Promega, Cat. No. E2720) on a white-bottom 96-well plate (Greiner ...
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bioRxiv - Immunology 2021Quote: ... then combined in a 1:1 ratio with Nano-Glo® Luciferase solution (Promega, Ref N1150). A Promega GloMax plate reader was used to measure luminescence.
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bioRxiv - Cell Biology 2022Quote: ... the samples were mixed in a 1:1 (v/v) ratio with luciferase assay reagent (Promega) in a 96-well white opaque plate and left at room temperature for 2 minutes ...
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bioRxiv - Neuroscience 2021Quote: ... 60 μL of each sample was mixed 1:1 with Steady-Glo Luciferase system (Promega #E2510) following the manufacturer’s protocols ...
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bioRxiv - Biochemistry 2020Quote: ... The attached cells were lysed in 20 μl of 1× passive lysis buffer (1× PLB, Promega) at room temperature (RT ...
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bioRxiv - Cell Biology 2022Quote: ... the samples were mixed in a 1:1 (v/v) ratio with nucleotide detection reagent (Promega) in a 96-well white opaque plate and left at room temperature in the dark for another 60 minutes ...
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bioRxiv - Cell Biology 2022Quote: ... the samples were mixed in a 1:1 (v/v) ratio with luciferase assay reagent (Promega) in a 96-well white opaque plate and left at room temperature for 2 minutes ...
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bioRxiv - Cell Biology 2020Quote: ... 1000 ng of donor and gRNA plasmids in 1:1 ratio was used using ViaFect (Promega) transfection reagent as per manufacturer’s guidelines ...
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bioRxiv - Neuroscience 2022Quote: ... rabbit anti-GFP 1:2000 (MBL, RRID: AB_591819) rabbit anti-Halo 1:1000 (Promega, RRID: AB_713650), rat anti-HA 1:500 (Sigma-Aldrich ...
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bioRxiv - Immunology 2022Quote: Caspase-1 activity was quantified using a Caspase-Glo 1 Inflammasome Assay kit (Promega, Madison, WI). THP-1 cells were treated and harvested as described above ...
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bioRxiv - Immunology 2023Quote: ... then combined in a 1:1 ratio with Nano-Glo® Luciferase solution (Promega, Ref N1150). A Promega GloMax plate reader was used to measure luminescence ...
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bioRxiv - Immunology 2024Quote: ... organoids were collected and lysed in 100 µl of a 1:1 dilution containing CellTiterGlo (Promega) and PBS ...
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bioRxiv - Cell Biology 2023Quote: ... For Non-SILAC samples, 1 µl trypsin (1 µg/µl Trypsin Gold, Mass Spectrometry Grade (Promega)) was added and samples were incubated at 37°C overnight ...
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bioRxiv - Immunology 2023Quote: ... then combined in a 1:1 ratio with Nano-Glo® Luciferase solution (Promega, Ref N1150). A Promega GloMax plate reader was used to measure luminescence in technical triplicate for each sample ...
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bioRxiv - Genetics 2023Quote: ... and for 1 hour at room temperature in secondary antibody (1:4,000 anti-Mouse-HRP, Promega). In order to detect the endogenous Ptiwi09 protein ...
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bioRxiv - Biophysics 2022Quote: ... mixed 1 to 1 (v / v) with 10 μg / ml trypsin (Sequencing Grade Modified Trypsin, Promega) in 50 mM ammonium bicarbonate and incubated for 2 hours at 60 °C for enzymatic hydrolysis ...
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bioRxiv - Cell Biology 2023Quote: ... Top half was incubated in anti-Nup107 (1:1000; ab236634) and HaloTag (1:500; Promega G9211). Bottom half was incubated in anti-GAPDH (1:1000 ...
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bioRxiv - Cancer Biology 2023Quote: ... organoids were stained using Hoechst 33342 (1 μg/mL) and CellTox Green (Promega, 1/20 000) dyes for 8 h prior to imaging at Opera Phenix (Perkin Elmer ...
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bioRxiv - Plant Biology 2024Quote: ... Aliquots #1 and #2 were then treated with 1 mg/mL trypsin (Promega, Madison, WI, USA) and incubated at 37 °C for 1 h followed by the addition of 1.5 mg/mL trypsin inhibitor (Worthington Biochemical Corp ...
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bioRxiv - Microbiology 2024Quote: Released caspase-1 enzymatic activity of was quantitated with the Caspase-Glo 1 inflammasome assay (Promega), following instructions from the supplier ...
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bioRxiv - Cell Biology 2024Quote: ... A polyclonal goat anti-mouse conjugated to horseradish peroxidase (1:5,000 or 1:10,000; Promega, W4021) was used as the secondary antibody ...
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bioRxiv - Biochemistry 2024Quote: ... cells were transfected with a 1:1 ratio of receptor construct and GloSensor22F cAMP biosensor (Promega) plasmids.
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bioRxiv - Genetics 2021Quote: ... 0.5 μL 1% Digitonin (Promega, G9441), 22 μL H2O ...
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bioRxiv - Molecular Biology 2021Quote: ... Size markers were 1 kb (Promega) and λ-HindIII (NEB ...
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bioRxiv - Molecular Biology 2021Quote: ... 1 μM HaloTag-TMR substrate (Promega), 0.25 U/μL Benzonase (Merck) ...
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bioRxiv - Cell Biology 2022Quote: ... 1 U/μl RNasin Plus (Promega)) ...
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bioRxiv - Cell Biology 2022Quote: ... 1 U/μl RNasin Plus (Promega) in 2x Saline-sodium citrate (SSC ...
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bioRxiv - Cell Biology 2022Quote: ... 1 μg of modified trypsin (Promega) was added ...
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bioRxiv - Genomics 2020Quote: ... 1% RNasin Plus RNase Inhibitor (Promega) was included in all buffers ...
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bioRxiv - Molecular Biology 2021Quote: ... 1% NLuc substrate (Nano-Glo, Promega), 16 pg/μl NLuc-mRNA and 0.6 μM Nsp1 protein or its mutants/domains or eIF1A as negative control ...
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bioRxiv - Biochemistry 2021Quote: ... and 1 μl dNTP (Promega, U1515) in NEBuffer 3 in a 50 μl reaction at 37°C for 60 min (25) ...
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bioRxiv - Systems Biology 2020Quote: ... donor (1 mM UDP-GlcNAc (Promega) for B3GNT2 ...
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bioRxiv - Microbiology 2021Quote: ... and 1 U DNase RQ1 (Promega) and incubated at 37°C for 15 minutes ...
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bioRxiv - Cell Biology 2020Quote: ... 1 μg of trypsin (Promega, # V5111) was then added to the samples and proteolysis was carried out overnight ...
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bioRxiv - Biochemistry 2022Quote: ... 25:1 (w/w) chymotrypsin (Promega), or 10:1 (w/w ...
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bioRxiv - Developmental Biology 2021Quote: ... 1 μL Pfu DNA Polymerase (Promega), and 200 μM dNTP mix (Promega ...
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bioRxiv - Molecular Biology 2020Quote: ... 1 µl dNTPs mix (Promega #U1515), 5 µl 10X DreamTaq Buffer (ThermoFisher #EP0703) ...
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bioRxiv - Cell Biology 2021Quote: ... 1 mM DTT and RNasin (Promega) 4U/ml ...
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bioRxiv - Microbiology 2021Quote: ... 1 mg/mL Proteinase K (Promega) with 0.5% SDS in PBS at 40 °C overnight ...
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bioRxiv - Biochemistry 2021Quote: ... Halo (Promega G9211, dilution 1:10000), SETD2 (Abclonal A3194 ...
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bioRxiv - Cancer Biology 2022Quote: ... or AP 1-driven luciferase (Promega) reporter plasmids ...
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bioRxiv - Microbiology 2022Quote: ... 1 unit of GoTaq enzyme (Promega) and 25 pmoles of pan-coronavirus primers (10) ...
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bioRxiv - Biochemistry 2020Quote: ... 7.5:1 (w/w) Chymotrypsin (Promega) or 5:1 (w/w ...
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bioRxiv - Microbiology 2021Quote: ... 1× GoTaq reaction buffer (Promega, USA), deoxynucleoside triphosphate (dNTP ...
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bioRxiv - Developmental Biology 2021Quote: ... 1/200 RNasin plus (Promega, N2611)).