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Citations for Promega :
1451 - 1480 of 1480 citations for Recombinant Human IL18RAP Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Systems Biology 2023Quote: ... proteins were precipitated with isopropanol50 and digested overnight at 37°C with 1:50 enzyme : protein ratio by Trypsin/Lys-C Mix (Promega GmbH, Germany). Resulting peptides were desalted on MicroSpin column (The Nest Group ...
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bioRxiv - Cancer Biology 2023Quote: ... The protein solution was spun onto an S-Trap device (Protifi, Farmingdale, NY) and digested by 750 ng trypsin (Promega, Madison, WI) in 100 mM TEAB buffer at 37°C overnight ...
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bioRxiv - Systems Biology 2023Quote: ... and the bound proteins were subjected to on-cartridge digestion with mass spec grade Trypsin/Lys-C Rapid digestion enzyme (Promega, Madison, WI) at 70°C for 2h ...
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bioRxiv - Molecular Biology 2023Quote: ... the RNA/Protein Purification Plus kit (Norgen, Thorold, ON, Canada) or the Maxwell 16 LEV simplyRNA purification kit (Promega, Madison, WI, USA) according to the manufacturers’ instructions ...
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bioRxiv - Cancer Biology 2023Quote: ... and the protein pellet was resuspended in 50 mM ammonium bicarbonate and digested with sequencing-grade modified trypsin (Promega, Madison, WI, USA) overnight at 37°C ...
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bioRxiv - Cell Biology 2024Quote: ... the heated plates were allowed to cool down on ice and proteins digested by adding 1µl of 10ng Trypsin Gold (Promega, catalog number V5280) in 100mM TEAB and incubating at 37°C overnight ...
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bioRxiv - Biochemistry 2024Quote: Purified polar flagellin proteins at a concentration of 0.5 µg/µL in 25 mM ammonium bicarbonate were digested with trypsin (Promega, Madison, WI, USA) using a protein to enzyme ratio of 30:1 (w/w ...
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bioRxiv - Neuroscience 2024Quote: ... stable cell line expressing the human MC4-R and a split luciferase cAMP genetic sensor based on the circularized fusion of firefly luciferase and protein kinase A regulatory cAMP binding domain (clone 22F, Promega, Madison WI) was used to determine the α-MSH-elicited cAMP responses in live cells as previously described 32 ...
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bioRxiv - Plant Biology 2020Quote: ... approximately 0.6 mg IgG-enriched proteins were first digested on the beads with mass spectrometry-grade Trypsin/Lys- C Mix (Promega, Madison, WI, USA) at a 1:50 (enzyme/substrate ...
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bioRxiv - Plant Biology 2021Quote: ... In vitro transcription and translation to radiolabel the proteins with [35S]-methionine was performed with the TnT® Quick Coupled Transcription/Translation System (Promega, Walldorf, Germany) in reticulocyte lysate ...
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bioRxiv - Microbiology 2021Quote: ... HepG2 or sg25 cells were transfected with a control plasmid or protein expression plasmids together with the luciferase reporter plasmids using Viafect TM Transfection Reagent (Promega, Madison, WI, USA) or Lipofectamine TM 2000 (Invitrogen ...
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bioRxiv - Plant Biology 2020Quote: ... and RPM1 CC-HA (1-156aa) were expressed in vitro using the TnT® SP6 High-Yield Wheat Germ Protein Expression System (Promega; Madison, USA) according to the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2020Quote: ... The protein mixture was diluted to 25mg/ml with 50mM ABC and 500ug of protein were digested with 2% w/w Trypsin (Promega, Cat. no. V5111) in 1mL of 50mM ABC overnight at 37°C ...
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bioRxiv - Biochemistry 2021Quote: The kinase activity of the FLT3-ITD protein was measured by using ADP-Glo™ Kinase Assay Kit (Promega, Madison, Wisconsin, the US). Briefly ...
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bioRxiv - Cancer Biology 2022Quote: ... Immunoprecipitated protein–DNA complexes and 5% input were analyzed by qRT-PCR using GO taq QPCR master mix (Promega, A6002, lot no. 0000385100) in triplicate using primers specific for PFKFB3 HREs ...
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bioRxiv - Molecular Biology 2023Quote: ... The resulting proteins with carbamidomethyl groups on their cysteines were digested with 300 ng of sequencing-grade porcine trypsin (Promega, Fitchburg, MA, USA) and injected on an Easy-nanoLC-1000 system coupled to a Q-Exactive+ mass spectrometer (Thermo Fisher Scientific ...
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bioRxiv - Biochemistry 2023Quote: The cell free protein expression assays were performed by using the TnT® Quick Coupled Transcription/Translation System (Cat#L1171, Promega, Madison, USA), according to the manufacturer’s guideline with minor modification ...
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bioRxiv - Biochemistry 2023Quote: ... each sample was hydrolyzed to 100 µg of protein in a 50: 1 ratio using trypsin (trypsin Gold, MassSpec Grade, Promega, Fitchburg, WI, USA). After enzymolysis at 37 for 14 h ...
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bioRxiv - Molecular Biology 2024Quote: ... In vitro transcribed and translated FLAG-tagged ERRα protein was made using the TnT reticulocyte reaction (Promega cat #L1170, 2h on 37°C) or the TnT wheat germ extract (cat #L5030 ...
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bioRxiv - Plant Biology 2024Quote: ... 25 µg of phloem sap and 50 µg of leaf tissue protein extract were then digested in solution using a Trypsin/Lys-C mixture (Mass Spec Grade, Promega, Madison, WI, USA) according to the instruction manual ...
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bioRxiv - Molecular Biology 2024Quote: ... pH 8.5 to a final urea concentration of 2 M for Trypsin/Lys-C based overnight protein digestion at 37 °C (0.5 µg protease, Mass Spectrometry grade, Promega Corporation, Cat No: V5072.)
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bioRxiv - Molecular Biology 2024Quote: ... Seventy micrograms of protein were digested overnight using the filter-aided sample preparation (FASP) method 23 with Trypsin/Lys-C mix (Promega, Madison, WI, USA) (enzyme-to-protein ratio 1:35 ...
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bioRxiv - Microbiology 2022Quote: ... the gel lane was cut into 10 bands and the proteins were subjected to in-gel digestion with trypsin (Promega, Madison, WI, United States) as previously described (Leijon et al. ...
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bioRxiv - Microbiology 2023Quote: ... Samples were diluted in 50 mM ammonium bicarbonate and normalized to 50 μg protein for overnight LysC/trypsin digestion (Promega, protein:enzyme ratio, 50:1). Trifluoroacetic acid (TFA ...
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bioRxiv - Cell Biology 2023Quote: ... pH 8.5 to a final urea concentration of 2 M for Trypsin/Lys-C based overnight protein digestion at 37 °C (40 µg of protein used for global proteomics and the remainder for phosphoproteomics, 1:70 protease: substrate ratio, Mass Spectrometry grade, Promega Corporation, Cat No: V5072.) Digestions were acidified with trifluoroacetic acid (TFA ...
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bioRxiv - Cell Biology 2021Quote: ... The samples were diluted with 20 mM HEPES pH 8.0 to a urea concentration of 2 M and the proteins were digested with 4 µl Trypsin/LysC (Promega V5073: 20ug + 80uL 50mM acetic acid) for 4 hours at 37°C and boosted with an extra 2µl Trypsin/LysC (Promega V5073 ...
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bioRxiv - Molecular Biology 2023Quote: ... 30 min at 60°C) an alkylation (IAA 0.5 M, 30 min RT) proteins were digested using trypsin (Gold, Promega, 1ug / sample, overnight at 37°C). For LC MSMS analysis ...
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bioRxiv - Cell Biology 2021Quote: ... 30 min at 60°C) an alkylation (IAA 0.5M, 30 min RT) microtubule-associated protein enriched fractions were digested using trypsin (Gold, Promega, 1 μg / sample, overnight at 30°C). Peptide clean-up was done using OMIX C18 (Agilent ...
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bioRxiv - Microbiology 2020Quote: ... alkylation was performed using 20 mM 2-chloroacetamide at room temperature in HEPES buffer for 30 min under exclusion of light. Samples were prepared according to the SP3 protocol (Hughes et al. 2019) and trypsinized (sequencing grade, Promega, enzyme to protein ratio 1:50) overnight at 37°C ...
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bioRxiv - Cancer Biology 2022Quote: ... the beads were washed in the ABC buffer (50mM ammonium bicarbonate, pH 8.3) and proteins digested on beads with TPCK-trypsin (Promega, Madison, WI, 16 hours at 37 °C). The supernatant containing the tryptic peptides was collected and lyophilized ...