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Citations for Promega :
1451 - 1500 of 5726 citations for Mouse Protein Jagged 2 JAG2 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2019Quote: ... 100 µg of protein was then added to 100 µl of prewashed HaloLink resin (Promega G1914), which was prepared by washing and pelleting for 2m at 800xg 3x in wash buffer (100 mM Tris pH 7.5 ...
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bioRxiv - Molecular Biology 2019Quote: Dried protein samples were digested in 200 mM EPPS buffer pH 8.5 with trypsin (Promega #V5111). Digests contained 2% acetonitrile (v/v ...
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bioRxiv - Developmental Biology 2021Quote: Proteins were synthesized with the TnT SP6 Quick Coupled Transcription/Translation System (Promega, Madison, Wisconsin, USA).
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bioRxiv - Microbiology 2019Quote: ... The gene that encodes the HaloTag protein was optimized for Magnetospirillum codons with permission from Promega Corporation (Madison ...
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bioRxiv - Microbiology 2021Quote: ... Proteins were subsequently digested off the beads by addition of 0.4 µg Trypsin/LysC (Promega, #V5072) for 1 h while shaking in a thermoshaker at 37°C ...
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bioRxiv - Molecular Biology 2019Quote: ... proteins were digested for 6 h at 37°C with 4ng.μL−1 of modified trypsin (Promega) dissolved in 50 mM NH4CO3 ...
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bioRxiv - Cell Biology 2019Quote: ... the bead-bound protein was incubated with either 6.7 µM HaloTag-AlexaFluor660 or HaloTag-TMR (Promega), or SNAP-Surface Alex Fluor 647 (NEB) ...
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bioRxiv - Neuroscience 2019Quote: ... Proteins were digested overnight at 37°C with 1 μg of mass spectrometry grade Trypsin (Promega). The resulting peptide samples were cleaned up for mass spectrometry in a Sep-Pak C18 column (Waters ...
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bioRxiv - Physiology 2019Quote: ... SDS was removed by chloroform/methanol precipitation and the proteins were proteolytically digested with trypsin (Promega) and purified using OMIX C18 Mini-Bed tips (Agilent Technologies ...
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bioRxiv - Developmental Biology 2020Quote: Tryptic digestion of bead-bound protein was performed in 5% w/w trypsin (Promega, cat. #V5280), 50mM triethylammonium bicarbonate buffer at 37 °C overnight ...
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bioRxiv - Developmental Biology 2020Quote: Unlabelled in vitro translated protein was produced using rabbit reticulocyte lysates according to manufacturer’s protocol (Promega).
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bioRxiv - Biochemistry 2022Quote: ... The protein from lysed cells were digested in 1 µL 10ng/µL of trypsin/LysC (Promega) in 100mM TEAB (ProtiFi) ...
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bioRxiv - Biochemistry 2022Quote: ... aliquots of EPO proteins were digested respectively using a combination of trypsin and Glu-C (Promega), or Glu-C (Promega) ...
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bioRxiv - Biochemistry 2022Quote: ... aliquots of EPO proteins were digested respectively using a combination of trypsin and Glu-C (Promega), or Glu-C (Promega) ...
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bioRxiv - Developmental Biology 2022Quote: ... On-pellet protein digestion was performed in 50 mM ABC (pH 8) by adding TrypLysC (Promega) at an enzyme ...
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bioRxiv - Molecular Biology 2022Quote: ... were incubated with 35S labeled proteins made by using TNT Quick Coupled transcription/Translation System (Promega) with 35S labeled methionine ...
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bioRxiv - Systems Biology 2022Quote: ... In-solution digestions were performed on lysates containing 5 mg of protein using either Trypsin (Promega), Chymotrypsin (Promega) ...
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bioRxiv - Molecular Biology 2023Quote: ... proteins were digested by 0.5 µg lysyl endopeptidase (LysC) (Fujifilm-Wako) and 0.5 µg Trypsin (Promega) at room temperature overnight ...
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bioRxiv - Microbiology 2022Quote: ... Protein was then digested with 0.5 µg Sequencing Grade Modified Trypsin (V5111 - Promega, Madison, Wisconsin, USA). Following the overnight digestion at 37°C ...
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bioRxiv - Plant Biology 2023Quote: ... proteins were alkylated with iodoacetamide and digested with Trypsin/Lys-C Mix (Promega, Madison, WI, USA), according to the manufacturer’s instructions ...
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bioRxiv - Systems Biology 2022Quote: ... The proteins were diluted to 0.1 M ammonium bicarbonate and digested overnight with Trypsin (Promega, V5113) at 37°C with a protein-to-enzyme ratio of 50:1 ...
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bioRxiv - Systems Biology 2024Quote: ... proteins were digested overnight at 37 °C and 800 rpm using sequencing-grade modified trypsin (Promega) at a protein-enzyme-ratio of 50:1 ...
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bioRxiv - Neuroscience 2024Quote: ... Protein samples (15 µg) were digested overnight at 37 °C with trypsin (Promega, Madison, WI-USA) in a protein:trypsin ratio of 50:1 (w/w) ...
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bioRxiv - Cancer Biology 2024Quote: ... the fusion proteins were expressed using the cell-free transcription and translation system (Cat. # L4140, Promega) following the manufacturer’s protocol ...
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bioRxiv - Biophysics 2023Quote: ... the proteins were digested into tryptic peptides by incubation with 1□µg sequencing grade trypsin (Promega) overnight at 37□°C ...
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bioRxiv - Neuroscience 2023Quote: ... Syp-Halo protein was incubated with 5 times molar excess halo ligand Alexa 488 (Promega, USA) overnight at 4°C ...
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bioRxiv - Molecular Biology 2023Quote: ... Protein aggregation was initiated by addition of Factor Xa protease (SKU PR-V5581, Promega, Madison, WI) at 22◦C ...
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bioRxiv - Neuroscience 2023Quote: ... After the sequential digestion with LysC and trypsin (Promega, each protease to protein ratio 1:80), beads were retained with a magnetic rack and samples were filtered with Costar Spin-X spin filters (0·22 µm cut-off ...
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bioRxiv - Synthetic Biology 2023Quote: ... Protein was quantified using the Nano-Glo® HiBiT Extracellular Detection System (N2420, Promega, Madison, WI) using the HiBiT Control Protein (20 µM ...
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bioRxiv - Biochemistry 2023Quote: ... and proteins were digested overnight with LysC (FUJIFILM Wako) and trypsin (sequence grade; Promega, Madison, WI) at the protein:enzyme ratio of 100:1 for each enzyme at 25 °C ...
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bioRxiv - Biochemistry 2023Quote: ... 10 µg of reconstituted protein was digested in 0.05 N HCl with 1 µg pepsin (Promega) without prior reduction/alkylation at a volume of 200 µl 50 °C for 3 h ...
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Short-range interactions between fibrocytes and CD8+ T cells in COPD bronchial inflammatory responsebioRxiv - Pathology 2023Quote: Proteins were digested by incubating each gel slice with 10 ng/µl of trypsin (V5111, Promega) in 40 mM NH4HCO3 ...
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bioRxiv - Cell Biology 2023Quote: ... 35S-labeled proteins were synthesized in vitro using the TnT T7 coupled reticulocyte lysate system (Promega). Translation reaction products from 0.25 μg of plasmid DNA were incubated with GST-labeled proteins on glutathione-Sepharose beads in NETN-E buffer (50 mM Tris ...
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bioRxiv - Microbiology 2023Quote: ... the pcDNA3.1+ expression plasmids for spike proteins were co-transfected using Fugene (Promega, Madison, WI, USA) into HEK293T/17 cells ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... LgBiT protein either purified in-house (His-LgBiT) or purchased from Promega (N401A, 200 µM stock) was diluted to 20 pM in PBS pH 7.4 solution and dispensed into white 96 well non-binding surface plate (Corning #3600 ...
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bioRxiv - Immunology 2024Quote: ... PKA activation in H9c2 cells was measured using SignaTECT cAMP-dependent protein kinase assay system (Promega) according to the manufacturer’s instruction ...
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bioRxiv - Biophysics 2024Quote: ... The purified fused protein was cleaved by treating it with Factor Xa (FXa; Promega, Madison, WI) at room temperature ...
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bioRxiv - Biochemistry 2024Quote: ... Proteins were digested with Lys-C (Wako Chemicals) and trypsin (1:40 ratio, Trypsin Gold, Promega). After complete digestion had been confirmed by nanoLC-MS/MS (<7% missed cleavage sites) ...
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bioRxiv - Systems Biology 2024Quote: ... pH 8.0 were added and proteins were digested with 1.5 μl trypsin (0.5 μg/μl; Promega) with constant shaking at 1,100 rpm and incubation at 37 °C on a ThermoMixer C incubator for 4 h ...
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bioRxiv - Biochemistry 2020Quote: ... GDP detection kit was from ProFoldin (MA, USA) and ADP-Glo™ Kinase Assay kit from Promega Corporation ...
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bioRxiv - Genetics 2022Quote: Genomic DNA was isolated from blood samples using commercial kit (Wizard® Genomic DNA Purification Kit; Promega), following the fabricant protocol ...
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MANF regulates unfolded protein response and neuronal survival through its ER-located receptor IRE1αbioRxiv - Cell Biology 2020Quote: ... Sigma-Aldrich)–coated dishes or glass coverslips with 30 ng/ml of 2.5 S mouse NGF (G5141, Promega) in the Neurobasal medium containing B27 supplement (17504044 ...
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bioRxiv - Neuroscience 2021Quote: ... followed by the incubation with a secondary antibody anti-mouse IgG HRP conjugate (W4021, Promega, U.S.A.). All the primary antibodies were diluted 1/3000 in 10% Blocking One/TTBS (50 mM Tris ...
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bioRxiv - Molecular Biology 2021Quote: Primary antibodies and the dilutions used are as follows: anti-Halo (mouse, Promega G9211, 1:200); anti-Ezh2 (mouse ...
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bioRxiv - Microbiology 2021Quote: ... immunoblots were probed with the appropriate secondary α-mouse antibody conjugated to horseradish peroxidase (Promega, W402B) at a dilution of 1:5000 ...
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bioRxiv - Cell Biology 2020Quote: ... Horseradish peroxidase (HRP)-coupled secondary antibodies directed against mouse or rabbit IgG were purchased from Promega. Chemiluminescence images were acquired using the ImageQuant LAS 500 system (GE Healthcare).
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bioRxiv - Cell Biology 2019Quote: HepG2 cells or frozen mouse liver were sonicated in PBS containing protease and phosphatase inhibitors (Promega) in an ice-water bath using a Branson 250 digital sonifier programmed to cycle at a 10% amplitude with 5 seconds on followed by 20 seconds off ...
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bioRxiv - Cancer Biology 2019Quote: ... were secondary incubated with anti-mouse IgG (H+L) horseradish peroxidase coupled (1:3,000, W402b, Promega) and polyclonal anti-rabbit IgG (1:5000 ...
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bioRxiv - Developmental Biology 2020Quote: ... Primary antibodies used were: rabbit and mouse anti-βGal (1/1000; Promega and MP Biomedicals, respectiveley), rat anti-Ser (1/1000 ...
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bioRxiv - Cancer Biology 2020Quote: ... and then with 1:5000 of anti-rabbit (#W401B) or anti-mouse (#W402B) secondary antibodies (Promega).