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Citations for Promega :
1451 - 1500 of 1829 citations for 3RS 4 Dimethylamino 3 methyl 2 2 diphenylbutanenitrile Isodidiavalo since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2024Quote: ... HeLa cells were co-transfected with 0.5 μg of “driver” plasmid (pTMO2F3) + 0.5 μg of pAluneoTet using 3 μL FuGENE® HD (Promega); the following day ...
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bioRxiv - Cancer Biology 2024Quote: Purified rRNA (1 to 3 µg) was digested overnight at 37 °C with 270 units of Nuclease S1 (Promega) using the supplied buffer ...
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bioRxiv - Immunology 2024Quote: ... and 1μg of plasmid expressing the respective spike protein using a 3:1 ratio of Fugene HD transfection agent (Promega).28,49 After 48 hours of incubation at 10% CO2 ...
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bioRxiv - Cell Biology 2024Quote: ... Cells were seeded onto acid-washed coverslips at a density of 3 × 104 cells per coverslip for 24 h before transfection with 1μg of plasmid DNA using 3 μl Fugene HD transfection reagent (Promega). HaloTagged AP-2 σ2 was visualized by adding Janelia Fluor 646-HaloTag ligand (Promega) ...
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bioRxiv - Cancer Biology 2024Quote: ... 24hr and 48 hr by addition of the Caspase-Glo 3/7 reagent according to the manufacturer’s protocol (Promega). Luminescence was detected by a microplate reader (TECAN) ...
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bioRxiv - Cancer Biology 2024Quote: ... plates were equilibrated to room temperature and 100 μL of room temperature Caspase-Glo 3/7 reagent (Promega G8091) was added to each well ...
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bioRxiv - Cancer Biology 2020Quote: ... Samples were diluted 4-fold prior to overnight digestion at 37 °C with trypsin (Trypsin Gold, Promega) at an enzyme to substrate ratio of 1:50 ...
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bioRxiv - Cell Biology 2022Quote: ... 4 μl of each reaction was spotted onto a streptavidin-coated SAM2 Biotin Capture Membrane (Promega, #TB547). The membrane was air-dried ...
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bioRxiv - Cancer Biology 2022Quote: ... Lysates were diluted 4-fold with 100 mM ammonium acetate and digested with sequence-grade trypsin (Promega) overnight at RT at an enzyme:substrate ratio of 50:1 (w/w) ...
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bioRxiv - Cancer Biology 2022Quote: ATRX knock-out clone were established by transfecting SK-N-AS and GI-ME-N with the Cas9 expressing vector containing ATRX_KO_sgRNA_2 (targeting exon 4) and the corresponding homology arm plasmid using Fugene HD transfection reagent (E2312, Promega). Three days after transfection ...
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bioRxiv - Genetics 2022Quote: ... Samples were diluted 1:4 with 50mM Tris/HCl (pH 8.0) and sequencing grade modified trypsin (Promega) was added in an enzyme-to-substrate ratio of 1:50 ...
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bioRxiv - Biochemistry 2023Quote: ... and assayed with 4 μM of HRP and 20 μL of Nano Glo luciferase substrate (Promega, N1110) diluted to 1:1000.
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bioRxiv - Cancer Biology 2023Quote: ... ZR-75-1 cells were short tandem repeat tested every 4 months (Stem Elite ID System, Promega). Doxorubicin (S1208) ...
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bioRxiv - Cancer Biology 2023Quote: ... Cell viability was measured at day 4 and day 6 using the Cell-Titer Glo assay (Promega). Viability data was analyzed by comparing the relative viability change between base editing with the uORF gRNA and the associated parental CDS gRNA ...
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bioRxiv - Systems Biology 2024Quote: ... samples were diluted 1:4 with 50 mM Tris–HCl pH 8 and sequencing grade trypsin (Promega) was added at 1:50 ratio ...
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bioRxiv - Molecular Biology 2024Quote: ... cells were lysed at 4 °C in RIPA buffer containing protease and phosphatase inhibitors cocktail (Promega/Sigma). Proteins were separated by SDS-PAGE and transferred onto 0.45 µm pore size Immobilon PVDF membrane (Millipore) ...
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bioRxiv - Synthetic Biology 2024Quote: ... after which they were treated with 1 µL RNase A (4 mg/mL RNAse A solution, Promega), and 1 µL RNase One (10 units/µL RNase ONE Ribonuclease solution ...
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bioRxiv - Biochemistry 2024Quote: ... 4 U/μl per reaction reverse transcriptase (Catalog no. M5301, Promega Biotech India Pvt. Ltd, New Delhi) was added with a buffer of 3 mM MgCl2 ...
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bioRxiv - Molecular Biology 2021Quote: ... Mtfp1 and Gnpat 3’UTRs and Tnksbp1 and Gnpat CDS were amplified by PCR from MIN6 cDNA and subcloned into pmirGLO (Promega), downstream the Firefly ORF ...
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bioRxiv - Developmental Biology 2020Quote: ... and ‘TA cloned’ (after addition of 3’ adenine nucleotide overhangs) it into pGEM®-T-Easy plasmid vector (Promega, A1360). The derived plasmid was mutagenised (commercial service ...
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bioRxiv - Biochemistry 2022Quote: HeLa and HEK293T cells were seeded on 18 mm glass coverslips at a density of 3×105 cells/mL and were transfected with FuGENE 6 (Promega) transfection reagent 24 h later ...
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bioRxiv - Neuroscience 2021Quote: ... reverse mutant primer oIMR1437 5′-TCC ACC TAG CCT GCC TGT AC-3′) with 1U GoTaq polymerase (Promega, Madison, USA), 1X green GoTaq buffer ...
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bioRxiv - Molecular Biology 2021Quote: ... 1 μL RRV-P3 plasmid or 3 μL of cDNA from rose tissue or nuclease-free water (Promega, Madison, WI). The final volume of the LAMP reaction was 25 μL.
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bioRxiv - Molecular Biology 2021Quote: RNA extraction from liver tissue (n=3-6 biological replicates per group) was performed using the ReliaPrep RNA Miniprep system (Promega), as per manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2020Quote: The non-TOP 5′ UTR sequence of ATP5O (5′-CGGGAGAAG-3′) was identified in published nanoCAGE data 90 and inserted between the T7 promoter and Renilla luciferase in psiCHECK2 (Promega) by Gibson Assembly.
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bioRxiv - Immunology 2021Quote: ... JEG-3 viability after 1 h salubrinal pretreatment followed by 48 h ZIKV infection was measured by CellTiter-Glo Assay (Promega) following the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2021Quote: ... Genomic regions flanking the CRISPR target sites were PCR amplified with designed primers (Supplemental Table 3) using GoTaq DNA polymerase (Promega) and sent for Sanger sequencing to determine the insertion and deletion errors generated by CRISPR-Cas9 system in exon 2 of PARKIN ...
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bioRxiv - Cell Biology 2021Quote: ... The cells were then washed three times with 1 × PBS and blocked with 3% Blot-Qualified bovine serum albumin (BSA, Promega) in 1 × PBS containing 0.05% Tween-20 (TPBS ...
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bioRxiv - Biochemistry 2020Quote: ... protein was modified with an poly(A)25 insertion at the 3’ end of the gene within the pRL-null vector (Promega). The gene encoding the NanoLuc luciferase was transferred from the pNL1.1 vector (Promega ...
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bioRxiv - Biochemistry 2020Quote: ... The membrane was probed with a 400 bp 32P-labelled PCR product of the TbCls 3’UTR generated with the prime-a-gene labelling system (Promega). Primers used for PCR were ...
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bioRxiv - Bioengineering 2020Quote: ... Reverse transcription into cDNA was done from 3 μg of RNA by using 500 μg/mL random hexamers (Promega, Switzerland) and 0.5 μL of 200 UI/mL SuperScript III reverse transcriptase (Invitrogen) ...
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bioRxiv - Neuroscience 2020Quote: Caspase 3/7 activity in primary neuronal cells was measured using ApoTox-Glo Triplex Assay kit (Promega, Madison, WI, USA) as per the manufacture’s instruction ...
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bioRxiv - Cancer Biology 2020Quote: ... The viability of cells was measured after 3 days incubation using a CellTiter-Glo Luminescent Cell Viability Assay kit (Promega) following the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2021Quote: ... To remove unwanted 3’phosphate groups from bound RNA fragments in both CLASH and FLASH the complexes were treated with TSAP phosphatase (Promega) using provided buffer for 40 min at room temperature ...
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bioRxiv - Microbiology 2020Quote: Huh7.5.1 cells were treated with different concentrations of RG7834 for 3 days and viability was measured using Cell Titer Glo (Promega) according to the manufacturer’s instructions.
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bioRxiv - Microbiology 2020Quote: Apoptosis induction by T3DC and inhibition by Z-VAD-FMK was determined using the Caspase-Glo 3/7 Assay System (Promega). PC3 cells were plated at 1 × 104 cells per 96 well plate and 24 h later were either treated with docetaxel ...
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bioRxiv - Microbiology 2021Quote: ... Cell viability was assessed 3 days postinfection by MTS assay using a Cell Titer 96 aqueous cell proliferation kit (Promega) or Viral ToxGlo™ (ToxGlo assay) ...
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bioRxiv - Cancer Biology 2020Quote: ... cells were treated in triplicates with increasing doses of in-house small molecule inhibitor drugs and a DMSO vehicle control for 3 days and subsequently assessed for cell viability by measurement of ATP with CellTiter-Glo Luminescent Cell Viability Assay (Promega). Signal intensity was read on a Glomax™ 96 Microplate Luminometer (Promega ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... cells were transfected using a 1:3 ratio of human μOR and a splitluciferase based cAMP biosensor (pGloSensorTM-22F; Promega). Transit 2020 (Mirus Biosciences ...
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bioRxiv - Molecular Biology 2021Quote: ... and 656 bp of murine Mef2d 3’UTR (pMIR-Mef2d) were subcloned into the pmirGLO Dual-Luciferase miRNA Target Expression Vector (Promega). Correspondent seed-sequence mutated Dual-pMIR-report plasmids were obtained using the QuikChange XL Site-Directed Mutagenesis Kit (Agilent Technologies) ...
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bioRxiv - Molecular Biology 2020Quote: For the analysis of cell vitality of Calu-3 cells treated with camostat mesylate or FOY-251 the CellTiter-Glo Luminescent Cell Viability Assay kit (Promega) was used ...
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bioRxiv - Microbiology 2020Quote: ... the target cells were resuspended at 3×105/mL in prewarmed culture medium that contains EnduRen live cell substrate (Promega) at a final concentration of 17 ng/ml and incubated for 30 min ...
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bioRxiv - Molecular Biology 2021Quote: ... and the pSARP-12R4-9 input library with a 3:1 transfection reagent:DNA ratio using Fugene 6 transfection reagent (Promega #E269A). At 72 hrs ...
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bioRxiv - Microbiology 2020Quote: ... and luciferase activity was measured in 3 µl aliquots of the cell lysates using a Dual-Luciferase Reporter Assay System (Promega). Firefly luciferase activity was normalized with that of Renilla luciferase.
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bioRxiv - Cell Biology 2021Quote: ... HEK293T cells were seeded in 24-well tissue culture plates at a density of 1.6 x 105 cells/well and transfected with 50-100 ng/well of pNL4-3-GFP using Fugene HD (Promega) transfection reagent ...
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bioRxiv - Biophysics 2020Quote: ... We stained the cells with 3-6 pM Halo-SiR ligand 48 directly before imaging according to the protocol of the manufacturer (Promega) to obtain equal molecular densities during imaging and therefore minimize differences in tracking losses.
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bioRxiv - Cell Biology 2020Quote: ... Membranes were washed 3 times for 15 min in TBST and subsequently incubated with species-specific HRP-conjugated secondary antibodies (1:10000; Promega) in 3% BSA-TBST for 1h at room temp ...
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bioRxiv - Molecular Biology 2022Quote: ... The SELENOP 3’UTR and the control plasmid were linearized using Bsb 1 and in vitro transcribed using the Ribomax kit (Promega). The RNA was then purified using p30 size exclusion columns (Bio-Rad ...
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bioRxiv - Microbiology 2022Quote: Templates for in vitro transcription of gene-specific 300-500 nt dsRNA and DENV2 EMSA probes were generated by PCR introducing a T7 promoter sequence or a universal tag at both 5’ and 3’ ends using the GoTaq Flexi DNA Polymerase (Promega). If present ...
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bioRxiv - Immunology 2022Quote: ... pH 8.0 using Vivaspin columns with a 3 kDa molecular weight cutoff and digested separately overnight using trypsin chymotrypsin or Glu-C (Mass Spectrometry Grade, Promega) at a ratio of 1:30 (w/w) ...