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Citations for Promega :
1451 - 1500 of 1953 citations for 2 Chloro 4 3 fluoro 4 hydroxyphenyl benzoic acid since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2024Quote: ... and codon optimized human PAH exon 2-13 coding sequence with HiBit reporter tag (Promega) and cloned into plasmid backbone with Gibson assembly ...
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bioRxiv - Biophysics 2024Quote: ... that was blocked for 2 hours in 5% milk TBST before being probed with (Promega) (CS2006A01 clone 30E5 ...
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bioRxiv - Cancer Biology 2024Quote: ... Tryptic digest was performed in 50 mM ammonium bicarbonate buffer with 2 μg trypsin (Promega) at 37°C overnight ...
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bioRxiv - Plant Biology 2020Quote: ... corniculatus plants was synthesized from 3 μg of total RNA using a Moloney Murine Leukemia Virus Reverse Transcriptase (MMLV-RT) (Promega, WI, USA) and 100 pmol of random hexamers (Pharmacia Biotech) ...
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bioRxiv - Cancer Biology 2021Quote: ... DNA:FuGENE complexes were formed at a ratio of 1:3 (μg DNA/μL FuGENE HD) according to the manufacturer’s protocol (Promega, Madison, WI, USA). The resulting transfection complex (1 part ...
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bioRxiv - Genomics 2021Quote: ... Cells were then grown for 3 days at which point the viability was measured using a CellTiter-Glo assay (Promega Cat# G9241) read out on a standard luminometer ...
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bioRxiv - Biochemistry 2020Quote: ... or PACAP (10 nM final) and assayed 3 hours later with the Nano-Glo® Dual-Luciferase® Reporter System (Cat# N1630; Promega) on a Berthold Sirius luminometer to quantify ERK activation.
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bioRxiv - Biophysics 2020Quote: ... P1KO cells were transiently transfected in a 6-well plate (the cells were seeded 8 h before transfection) in the presence of ruthenium red (10 μM) and mouse Piezo1 (3 μg) using Fugene6 (Promega, Madison, WI). 20–30% of cells showed positive GFP expression indicating successful Piezo1 transfection.
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bioRxiv - Bioengineering 2022Quote: ... The DNA transfection reagents were prepared by incubating 3 µg of plasmid DNA encoding the Bxb1 recombinase and 6 µL of Fugene6 reagent (Promega Cat # E2693) in 300 µL of Opti-MEM for 15 minutes and then added to the cell suspension ...
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bioRxiv - Biochemistry 2022Quote: The 4330bp PCR product amplified from M13mp18 by using primers: oM13-5-27 and oM13-3-24 was inserted into pGEM®-T Easy(PROMEGA #A137A). The resultant plasmid is named as pM13 ...
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bioRxiv - Microbiology 2022Quote: ... PAO1-WT and few colonies that grew in 3 mM 22D-chelated condition were isolated and gDNA extracted using Wizard® Genomic DNA Purification Kit (Promega, USA) for use in whole genome sequencing ...
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bioRxiv - Neuroscience 2022Quote: ... and mouse cholecystokinin (CCK, 3-688 of NM_031161) were PCR-amplified and subcloned into the pGEM-T Easy vector (Promega, Fitchburg, WI, USA). Digoxigenin (DIG)-labeled anti-sense probes were prepared using DIG RNA Labeling Mix (Sigma ...
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bioRxiv - Molecular Biology 2020Quote: ... The NanoLuc and Fluc luciferase activities were measured 3 days after transfection using the Nano-Glo® Dual-Luciferase® Reporter Assay System (Promega). The firefly (Fluc ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... RBD-R: 5′-TGC CTC GAG CTC AAG TGT CTG TGGATC AC-3′) into pGEM T-easy vector (Promega, Madison, WI, USA). A standard curve was generated by determining the copy numbers derived from serial dilutions of the plasmid (103–109 copies) ...
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bioRxiv - Microbiology 2020Quote: ... 3’-untranslated region (3’-UTR) fragment of IκBα (human/rat) was inserted into the pGL3-basic vector (firefly luciferase; Promega, Madison, WI, USA), which was obtained from General Biosystems (Anhui ...
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bioRxiv - Cancer Biology 2022Quote: ... we followed the genotyping method for KrasG12D mutation previously described.3 Genomic DNA was isolated from formalin fixed paraffin embedded thymus slides using ReliaPrep FFPE gDNA MiniPrep System (Promega, Madison, WI). The DNA was then amplified using polymerase chain reaction (PCR ...
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bioRxiv - Cancer Biology 2022Quote: Apoptosis induction in response to SRRM1 silencing in leukemia cell lines (25,000 cells/well onto white-walled multiwell luminometer plates) was performed by using Caspase-Glo® 3/7 Assay (Promega Corporation, #G8091) as previously reported (67) ...
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bioRxiv - Biochemistry 2021Quote: The promoter fragment of luciferase reporter plasmid pGADD34-0.5k generated by Eurofins Japan (Tokyo, Japan) was subcloned into a pGL-3 vector (Promega, Madison, WI, USA) by restriction enzyme cutting site KpnI/HindIII ...
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bioRxiv - Molecular Biology 2021Quote: ... A total of 24 clones (4 holobionts x 3 replicates x 24 clones = three 96-well plates) from each amplicon were amplified directly with GoTaq polymerase (Promega, Lyon France) in a 25 μL reaction volume using the following PCR parameters ...
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bioRxiv - Cell Biology 2022Quote: ... Control and treated cells were cultured for 1 day in 96-well plates then treated with small molecules for 3 consecutive days before incubation with 120 μL fresh media containing 20 μL of CellTiter 96® AQueous One Solution (Promega, G3580) for 1 to 4 hours at 37°C in a humidified ...
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bioRxiv - Molecular Biology 2022Quote: ... SEA (0.2 mg/ml) and soluble schistosomula antigens (0.2 mg/ml) was determined using the Caspase-Glo® 3/7 Assay System (Promega, Sydney, Australia) according to the manufacturers’ instructions.
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bioRxiv - Neuroscience 2023Quote: ... Cells were transfected with 1 µg of DNA diluted in 45 µl dMEM and then mixed with 3 µl ViaFect™ (Promega, USA) transfection reagent added and incubate for 20 minutes ...
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bioRxiv - Cell Biology 2022Quote: ... approximately 200,000 HEK-293T cells were transfected with 1 μg of plasmid DNA complexed to 3 μL of FuGENE HD (Promega, Cat: E2311). 16-20 hours post transfection the cells were dissociated from the plastic using cell dissociation buffer (Gibco Cat ...
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bioRxiv - Microbiology 2023Quote: The quantification of viral RNA was carried out in one-step RT-qPCR using 3 µl of the eluted RNA with the GoTaq® Probe One-step RT-qPCR System (Promega, Poland). This procedure employed in-house primers and a probe specifically tailored for the HCoV-229E N sequence ...
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bioRxiv - Evolutionary Biology 2023Quote: The 419 bp sequence of 3’UTR containing predicted target sites for miR-252 in CcTRPM was cloned into the pmirGLO vector (Promega, Wisconsin, USA) downstream of the luciferase gene to construct the recombinant plasmid of CcTRPM-3’UTR-pmirGLO using the pEASY®-Basic Seamless Cloning and Assembly Kit ...
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bioRxiv - Plant Biology 2024Quote: ... The transfected protoplasts were aliquoted into a 96-well plate with 200 nM D-luciferin/well with 3 biological replicates and then measured with a luminometer (Promega Max NAVIGATOR) every hour for 16 h.
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bioRxiv - Cancer Biology 2024Quote: Caspase-3/7 and -8 activities were assayed using Caspase-Glo®- 3/7 and -8 assays according to manufacturer’s instructions (Promega, Madison, WI).
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bioRxiv - Cell Biology 2020Quote: ... or SL1-deleted SARS-CoV-2 5’ UTR luciferase reporter plasmid using FuGENE Transfection Reagent (Promega). Luciferase assays were performed using the Luciferase Assay System (Promega) ...
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bioRxiv - Genetics 2020Quote: ... 2.4 µl of RQ1 DNase Buffer and 2 µl of RQ1 RNase-Free DNase (M6101, Promega) was added to each RNA sample and incubated for 15 minutes at 37°C ...
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bioRxiv - Evolutionary Biology 2020Quote: ... cDNA was synthesized from 2 μL of the eluted RNA using M-MLV reverse transcriptase (Promega) and random hexamer primers ...
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bioRxiv - Developmental Biology 2021Quote: ... and the resulting mRNAs (2 μg) were translated in 50 μl of rabbit reticulocyte lysate (Promega). Flag-tagged proteins were purified by immunoprecipitation with anti-Flag M2 antibody (Sigma ...
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bioRxiv - Molecular Biology 2021Quote: ... Viral RNA of SARS-COV-2 was detected by TaqMan®-based Real-Time PCR (Promega) using a CDC protocol with two sets of primer/probe which amplifies virus nucleocapsid (N ...
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bioRxiv - Molecular Biology 2021Quote: ... thiopropyl sepharose beads were incubated overnight at 37°C with 2 μg trypsin Gold (Promega, #V5280) in 50 mM NH4HCO3 ...
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bioRxiv - Genetics 2021Quote: ... The purified DNA was PCR amplified using 2× GoTaq Colorless Master Mix (Promega, Madison, WI, USA), and PCR product was extracted as above to eliminate primer-dimers ...
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bioRxiv - Plant Biology 2021Quote: ... 8 µL of a mix consisting of 2 µL deoxyribonucleotide triphosphate (dNTPs, Promega, Madison, Wisconsin, US), 4 µL 5x RT-buffer ...
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bioRxiv - Plant Biology 2020Quote: ... were digested for 12 hr at 37 °C with 2 mg of sequencing-grade trypsin (Promega), followed by a second incubation for 6 hr with an additional 2 mg of trypsin ...
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bioRxiv - Plant Biology 2020Quote: ... cDNA was synthesized from 2 μg of total RNA with the MMLV kit (Promega, Madison, WI). Semi-quantitative PCR was performed within the linear amplification phase using a recombinant Taq Polymerase (Invitrogen ...
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bioRxiv - Plant Biology 2020Quote: ... 2 μg RNA was used for cDNA synthesis with M-MLV Reverse Transcriptase (Promega, Madison, USA) and 1 μL of the obtained cDNA was used for real-time PCR with SYBR™ green master mix (Bioline ...
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bioRxiv - Microbiology 2020Quote: ... The lysate was then diluted to <2 M urea and incubated with 50 μg trypsin (Promega) overnight at 37 °C under agitation ...
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bioRxiv - Microbiology 2020Quote: ... Urea was diluted to 2 M with 100 mM Tris-HCl pH 7.0 and trypsin (Promega) was added at a mass ratio of 1:100 (trypsin:protein) ...
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bioRxiv - Neuroscience 2020Quote: ... all cells were incubated in the following: mouse anti-βIII tubulin (1:1000; 2 hr; Promega) and goat anti-mouse Alexa 546 (1:1000 ...
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bioRxiv - Microbiology 2022Quote: ... 2 μl of the gDNA were added to a GoTaq® Green Master Mix (Promega, USA) and used to amplify part of the mitochondrial cytochrome oxidase 1 (CO1 ...
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bioRxiv - Cell Biology 2022Quote: The SARS-CoV-2 spike pseudotyped virus activity was determined by bright-glo luciferase assay (Promega). The plate reader detected the luminescence two days post virus infection or without virus infection ...
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bioRxiv - Microbiology 2020Quote: ... JAK2 and MAP3K2 are cloned downstream of Renilla luciferase (Rluc) in the modified psiCHECK-2 (Promega) plasmid p5522 using the Gateway cloning technology (m Fisher Scientific ...
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bioRxiv - Molecular Biology 2020Quote: ... hepatica calmodulin 2 (fhcam2, AM412547) were generated using the T7 RiboMAX™ Express RNAi System (Promega) with primers listed in Supplementary Table 2 ...
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bioRxiv - Cancer Biology 2021Quote: ... BT-549 and HCC38 cells were transfected with both plasmids simultaneously (2 μg) using FuGene (Promega) according to the manufacturer’s instructions ...
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Genome modularization reveals overlapped gene topology is necessary for efficient viral reproductionbioRxiv - Synthetic Biology 2020Quote: ... 30 μg of protein was taken for digestion with trypsin (2% (w/w)) (Promega, United States) and left overnight at 37°C (∼18-hours) ...
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bioRxiv - Microbiology 2020Quote: ... incubated in the dark for 2 mins and read on a Glomax Multi+ Detection System (Promega).
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bioRxiv - Microbiology 2020Quote: ... 18h (SARS-CoV-2) or 24h (SARS-CoV) later by adding 1μM of Coelenterazine-H (Promega) at 1:400 dilution in PBS ...
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bioRxiv - Systems Biology 2021Quote: ... Both 1° and 2° assays were performed with 2x PCR master mix (Promega Corporation, Wisconsin, USA). The final PCR product from the 2° nested PCR was checked by electrophoresis in a 1.5% agarose gel ...