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Citations for Promega :
101 - 150 of 3110 citations for Mumps virus lysate Isolate 1 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2021Quote: 16S rRNA gene of each isolate was amplified from its genomic DNA using GoTaq G2 Hot Start Master Mix (Promega) and the purified PCR products were sequenced using BigDye Terminator v3.1 Cycle Sequencing Kit according to a previous report [15].
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bioRxiv - Microbiology 2020Quote: Genomic DNA from each isolate to be sequenced was purified using the Wizard Genomic DNA Purification Kit (Promega, Fitchburg, WI) and libraries were constructed and indexed using NexteraRTM DNA Library Prep Kit (Illumina ...
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bioRxiv - Genomics 2021Quote: Total genomic DNA of bacterial isolates was extracted using the Wizard® Genomic DNA Purification Kit (Promega, Madison, WI, USA) as per the manufacturer’s instructions ...
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bioRxiv - Systems Biology 2019Quote: ... To reduce the viscosity of the lysate and remove contaminating DNA the lysate was incubated with RQ1 DNase I (10U/ml Promega, M6101) for 30 minutes on ice ...
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bioRxiv - Synthetic Biology 2021Quote: ... 1 µl of this crude lysate was used for PCR in a 10 µl GoTaq Green reaction (Promega M7123) with 0.25 µM of primers ...
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bioRxiv - Cancer Biology 2021Quote: ... 1 mg/mL 50 μL aliquots of cleared lysates were incubated with 2 μL of indicated trypsin concentration (Promega) at 4°C for 15 min ...
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bioRxiv - Microbiology 2022Quote: ... A total of 56 Fusobacterium isolates were recovered and subjected to DNA extraction using the Wizard genomic extraction kit (Promega, USA). For each isolate ...
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bioRxiv - Biochemistry 2020Quote: ... Complementary DNA was reverse-transcribed using Moloney murine leukemia virus reverse transcriptase (Promega, Wisconsin, USA). Relative expression of genes of interest were quantified by real-time PCR using TaqMan™ gene expression assays (Mm02619580_g1 ACTB ...
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bioRxiv - Plant Biology 2021Quote: ... and 2μg RNA were transcribed into cDNA using Moloney murine leukemia virus reverse transcriptase (Promega). A 20-fold dilution of the cDNA reaction was used for PCR amplification with the ABsolute QPCR SYBR Green Capillary Mix (Thermo Scientific ...
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bioRxiv - Microbiology 2020Quote: ... and cDNA was produced with Moloney murine leukemia virus reverse transcriptase (M-MLV RT) (Promega) and random hexamers ...
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bioRxiv - Animal Behavior and Cognition 2021Quote: ... RNA was then reverse transcribed using Moloney-Murine Leukaemia Virus (M-MLV) reverse transcriptase (Promega) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2022Quote: ... and DNase-I digested before murine leukemia virus (MLV) reverse transcription using random primers (Promega). qPCR was performed using the TaqMan technology (Life Technologies) ...
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bioRxiv - Molecular Biology 2020Quote: The clarified lysate was incubated with HaloLink Resin (Promega) for 15min at 22°C with rotation ...
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bioRxiv - Microbiology 2021Quote: ... cell lysates were harvested in Passive Lysis Buffer (Promega) and analyzed with the Dual-Luciferase Reporter Assay System (Promega ...
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bioRxiv - Microbiology 2021Quote: ... cell lysates were harvested in Reporter Lysis Buffer (Promega) and analyzed with the Luciferase Assay System (Promega ...
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bioRxiv - Biochemistry 2020Quote: ... Nuclease-treated rabbit reticulocyte lysate translation reactions (Promega L4960) were set up in a 384-well plate (Thermo Scientific 164610 ...
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bioRxiv - Biochemistry 2021Quote: ... were performed in nuclease-treated rabbit reticulocyte lysate (Promega) as previously described (Zong et al. ...
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bioRxiv - Cell Biology 2020Quote: ... NDUF13 precursors were synthesized using rabbit reticulocyte lysate (Promega) in the presence of [35S] methionine ...
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bioRxiv - Biochemistry 2021Quote: ... 17.5 µL of nuclease-treated rabbit reticulocyte lysate (Promega), 5 U RNase inhibitor (Invitrogen) ...
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bioRxiv - Biochemistry 2021Quote: ... Nuclease-treated rabbit reticulocyte lysate was from Promega (L4960), [35S] methionine (EasyTag EXPRESS 35S Protein Labelling Mix ...
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bioRxiv - Cell Biology 2020Quote: ... Cell lysates were incubated with Stop & Glo substrates (Promega) and luciferase activity was measured in a Synergy Neo2 reader (Biotek).
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bioRxiv - Microbiology 2020Quote: ... the TnT® Coupled Reticulocyte Lysate system (Promega; #L4610) was utilised as per the manufacturers’ instructions using 200ng of plasmid DNA ...
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bioRxiv - Immunology 2021Quote: ... All transcripts were translated in rabbit reticulocyte lysate (Promega) in the presence of L-35S-methionine (Perkin Elmer) ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... each sample was added to Rabbit Reticulocyte Lysate (Promega) and incubated at 37°C for 15 minutes ...
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bioRxiv - Microbiology 2022Quote: ... 47.5 µL of nuclease-treated rabbit-reticulocyte lysate (Promega), and one of four concentrations of HR1 peptide (0 ...
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bioRxiv - Microbiology 2022Quote: ... were performed in nuclease-treated rabbit reticulocyte lysate (Promega) as described53,54 ...
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bioRxiv - Microbiology 2023Quote: ... Cell lysates were prepared using Passive lysis buffer (Promega). Supernatants were collected and measured for luciferase activities using the Dual luciferase reporter assay system (Promega) ...
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bioRxiv - Cell Biology 2023Quote: ... transcripts were incubated with rabbit reticulocyte lysates (L4960; Promega) for 30 min at 37°C.
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bioRxiv - Biochemistry 2024Quote: ... Normalized cell lysate was first mixed with vivazine (Promega) to reach 1% vol/vol as recommended by Promega ...
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bioRxiv - Cancer Biology 2022Quote: ... The lysate was washed in Nuclei wash/resuspension buffer (1xPBS completed with 1% BSA and 0.2U/ul RNAsin Plus (Promega, #N2611) and passed through a 40 μm cell strainer ...
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bioRxiv - Immunology 2020Quote: ... the cells were washed with PBS and then lysates were prepared by scraping the cells from plates in the presence of 1× Passive lysis buffer (Promega). Luciferase assays were performed according to Dual-Luciferase Assay System (Promega ...
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bioRxiv - Neuroscience 2023Quote: ... We performed targeted PCR by adding 1 μL of cell lysate (1:5 dilution) to a 25-μL PCR reaction containing GoTaq Hot Start Master Mix Green (Promega) and 0.5 μL of the primers (10 µM ...
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bioRxiv - Cancer Biology 2023Quote: ... Alkylated proteins were then cleaved using a two-step digestion: first with Endoproteinase Lys-C (ratio 1:33 enzyme: lysate, Promega) for 1 hour at 37 °C then with Trypsin (ratio 1:33 enzyme ...
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bioRxiv - Immunology 2022Quote: Viral RNA was extracted from virus-infected culture medium by using Maxwell RSC Instrument (Promega, AS4500). cDNA preparation and amplification were done in accordance with protocols published by the ARTIC network (https://artic.network/ncov-2019 ...
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bioRxiv - Molecular Biology 2021Quote: ... Renilla luciferase is driven by a constitutively active simian virus 40 (SV40) promoter (pRL-SV40; Promega), which served as a control for cell viability and transfection efficiency ...
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PSGL-1 inhibits HIV-1 infection by restricting actin dynamics and sequestering HIV envelope proteinsbioRxiv - Microbiology 2020Quote: ... Virus infectivity was assayed by measuring chemiluminescent firefly luciferase activity using the commercial kit from Promega.
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bioRxiv - Microbiology 2021Quote: All virus stocks were produced by plasmid transfection of HEK 293T cells with Fugene 6 (Promega). Supernatants were harvested at 48h and 72h ...
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bioRxiv - Cell Biology 2021Quote: ... as much liquid was removed as possible and HaloTEV (Tobacco Etch Virus) protease (300 units, Promega) was added to liberate protein ...
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bioRxiv - Cell Biology 2022Quote: The SARS-CoV-2 spike pseudotyped virus activity was determined by bright-glo luciferase assay (Promega). The plate reader detected the luminescence two days post virus infection or without virus infection ...
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bioRxiv - Microbiology 2020Quote: The total RNA was reverse transcribed into cDNA using Moloney murine leukemia virus reverse transcriptase (Promega). Real-time PCR was performed using the POWER SYBR green PCR master mix (Applied Biosystems ...
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bioRxiv - Immunology 2019Quote: ... Reverse transcription (RT) was carried out using 2.5 U avian myeloblastosis virus (AMV) reverse transcriptase (Promega) and a 32P-labelled primer in the presence of 4 mM MgCl2 and 0.5 mM dNTPs ...
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bioRxiv - Microbiology 2020Quote: ... Cells transduced with luciferase expressing virus were assessed using Promega Steady-Glo system (Promega Madison, WI)
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bioRxiv - Molecular Biology 2023Quote: ... Reverse transcription was then performed with 50 U of Avian Myeloblastosis Virus reverse transcriptase (Promega Corporation) for 20 min at 37 °C ...
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bioRxiv - Cancer Biology 2023Quote: Reverse transcription was performed using the Moloney Murine Leukemia Virus Reverse Transcriptase (Promega, Madison, WI, USA), 1000 ng of total RNA and random hexamers ...
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bioRxiv - Immunology 2023Quote: ... Pseudotyped virus were prepared by transfection of HEK293T cells using the Fugene HD transfection system (Promega), as previously described(42) ...
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bioRxiv - Biochemistry 2023Quote: ... 25 μL diluted lysate was mixed with an equal volume of Renilla-Glo Luciferase Assay System (prepared 1:100 as recommended; Promega # E2710) for 10 min at room temperature.
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bioRxiv - Molecular Biology 2021Quote: 500 µl rabbit reticulocyte lysate obtained from Promega (L4151, untreated) was supplemented with 1 µl 12.5 mM Hemin (Sigma ...
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bioRxiv - Biochemistry 2019Quote: ... using the TnT Quick-Coupled reticulocyte lysate system (Promega #L1170) and 35S-Methionine (Perkin Elmer #NEG709A001MC) ...
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bioRxiv - Cell Biology 2019Quote: ... and lysates were analyzed using a GloMax® Luminometer (Promega). Transfection and expression efficiencies were normalized to the activity of β-galactosidase activities.
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Selective inhibition reveals the regulatory function of DYRK2 in protein synthesis and calcium entrybioRxiv - Biochemistry 2021Quote: ... The cell lysates were firstly digested with trypsin (Promega, USA) by the in-solution digestion method22 ...