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Citations for Promega :
101 - 150 of 6959 citations for Mouse Eukaryotic Translation Initiation Factor 4E Binding Protein 1 EIF4EBP1 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2022Quote: In vitro generated RNA transcripts above were used templates for in vitro translation assays using a Rabbit Reticulocyte Lysate kit (Promega) at the indicated concentrations ...
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bioRxiv - Molecular Biology 2020Quote: ... We performed in vitro transcription/ translation using the TNT system (Promega) with pcDNA3 expression vectors for murine Flag-tagged Tet catalytic domains or full-length Halo-tagged human Tet proteins ...
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bioRxiv - Molecular Biology 2021Quote: ... coli employed a coupled T7/S30 in vitro translation system (Promega). A ~450 bp fragment containing the EMCV PRF signal (or mutant derivative ...
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bioRxiv - Cell Biology 2022Quote: ... generated using the TNT T7 Quick Coupled Transcription/Translation System (Promega), was imported into isolated mitochondria for 20 min at RT and import was stopped by the addition of 0.5 μM valinomycin ...
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bioRxiv - Molecular Biology 2020Quote: The coupled Transcription/Translation system (T7 Quick for PCR DNA, Promega) was used to express ATFS-1 from a PCR template ...
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bioRxiv - Cell Biology 2020Quote: ... the T7 TnT Quick coupled in vitro transcription/translation system (Promega) was used according to the suppliers instructions.
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bioRxiv - Biophysics 2020Quote: ... using the nuclease treated RRL in vitro translation system from Promega. The RRL was made more cap-dependent by addition of 75 mM KCl (58) ...
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bioRxiv - Biochemistry 2023Quote: ... and mRNA translation started by adding 50 ng FLuc mRNA (Promega), followed by incubation at 30 °C for 2 hours ...
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bioRxiv - Molecular Biology 2023Quote: ... Protein aggregation was initiated by addition of Factor Xa protease (SKU PR-V5581, Promega, Madison, WI) at 22◦C ...
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bioRxiv - Biophysics 2023Quote: ... The purified fused protein was cleaved by treating it with Factor Xa (FXa; Promega, Madison, WI) at room temperature ...
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bioRxiv - Developmental Biology 2021Quote: ... pCDNA-Cnot3 Δ701-751 and pCDNA-Cnot3Δ651-751 were in vitro transcribed/translated using a TNT® Quick Coupled Transcription/Translation kit (Promega, USA) according to manufacturer’s instructions using 10μCi of 35S-Methionine to radiolabel the proteins ...
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bioRxiv - Cell Biology 2019Quote: ... Translations were performed using the Flexi® Rabbit Reticulocyte Lysate System (Promega) supplemented with DTT (10 mM ...
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bioRxiv - Biochemistry 2019Quote: Translations were performed using the Flexi® Rabbit Reticulocyte Lysate System (Promega) supplemented with 10 mM DTT (Melford ...
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bioRxiv - Molecular Biology 2021Quote: In vitro translation in RNase-treated Rabbit Reticulocyte Lysate System (RRL, Promega) was carried out as recommended by the manufacturer with slight modifications ...
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bioRxiv - Physiology 2022Quote: ... The TnT T7 Quick Coupled Transcription/Translation System (catalog no. L1170, Promega) was used with pcDNA3.1(+)-FLAG-mGCNA and pcDNA3.1(+)-FLAG-mSPRTN E113Q plasmids in a 40 μl reaction according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: Coupled transcription/translation reactions were carried out per manufacturer’s instructions (Promega, L1170). Briefly ...
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bioRxiv - Immunology 2023Quote: ... TnT® T7/SP6 Quick Coupled Transcription/Translation System (L1170/L2080) (Promega).
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bioRxiv - Immunology 2024Quote: ... FC tags on the S and RBD proteins were removed through treatment with Factor Xa Proteinase (Promega).
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bioRxiv - Cell Biology 2019Quote: ... or EED that was in vitro-transcribed and translated according to the manufacturer’s procedures (TNT T7 Quick Coupled Transcription/Translation Kit; Promega, Leiden, the Netherlands) at 4°C overnight ...
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bioRxiv - Cancer Biology 2020Quote: In vitro translation of full-length SREBP1a and full-length SREBP1c was carried out using the TNT rabbit reticulocyte lysate translation kit (Promega, Wisconsin, USA) as per the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2019Quote: ... In vitro translation reactions were performed in nuclease-treated Rabbit Reticulocyte Lysate (Promega) as described by the manufacturer ...
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bioRxiv - Cancer Biology 2019Quote: ... Translation read-out was performed using the dual-luciferase reporter assay system (Promega) as per the manufacturer’s protocol on a Tecan M200 multimode reader running Tecan Magellan software (Tecan) ...
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bioRxiv - Plant Biology 2021Quote: ... The MYB -expression vectors were used in a coupled transcription/translation system (Promega) to produce MYB Halo tagged proteins ...
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bioRxiv - Plant Biology 2021Quote: ... and the TnT® Quick Coupled Transcription/Translation System (for T7 promoter, Promega) according to the manufacturers protocol ...
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bioRxiv - Plant Biology 2021Quote: ... The MYB24-expression vector was used in a coupled transcription/translation system (Promega) to produce MYB24 protein with the HaloTag-fusion ...
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bioRxiv - Molecular Biology 2023Quote: In vitro translation was performed using a rabbit reticulocyte lysate system (Promega, L4960). 1.5 μL of purified eIF2A protein or BSA was added to 10 μL rabbit reticulocyte lysate reaction mix ...
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bioRxiv - Biochemistry 2023Quote: In vitro translation assays were performed using nuclease-treated rabbit reticulocyte lysate (Promega). Reactions were in 10 µl containing 2 µl RRL ...
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bioRxiv - Plant Biology 2023Quote: ... we used in vitro translation system based on Wheat Germ Extract (Promega Corporation), which has been used for SCR validation as reported previously31 ...
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bioRxiv - Molecular Biology 2024Quote: In vitro translation was performed using a rabbit reticulocyte lysate system (Promega, L4960). Capped and polyadenylated reporter mRNAs containing the first 135 nucleotides of NPR coding sequences (human ...
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bioRxiv - Immunology 2020Quote: ... were verified by Sanger sequencing and used as DNA templates in the T7 TNT in vitro transcription/translation kit (Promega, Madison, WI; #L1170) using [35S]-methionine (PerkinElmer ...
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bioRxiv - Neuroscience 2021Quote: ... Myc-tagged MiD49 and MiD51 were produced via in vitro transcription/translation (IVT) (Promega). For in vitro ubiquitination assays ...
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bioRxiv - Biophysics 2020Quote: ... In vitro translation of the mRNA was carried out using rabbit reticulocyte lysate (Promega) supplemented with canine rough microsomes (tRNA probes ...
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bioRxiv - Plant Biology 2021Quote: Reactions were performed using the TNT® T7 Quick Coupled Transcription/Translation System (Promega) with 1 μg of vector (NPH3 or variants in pGADT7 ...
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bioRxiv - Biochemistry 2020Quote: ... except that the efficiency of translation of 100 ng/μl firefly luciferase mRNA (Promega) in RRL was measured (in relative luminescence units ...
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bioRxiv - Microbiology 2020Quote: ... then labeled with 35S-Methionine (Amersham Pharmacia Biotech) in vitro transcription-translation system (Promega), respectively ...
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bioRxiv - Cell Biology 2022Quote: ... In vitro translation was done using the TNT T7 Reticolucyte Lysate System (Promega Corp.), in the presence of radioactive 35S-methionine ...
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bioRxiv - Molecular Biology 2021Quote: In vitro translation reactions were performed using nuclease-treated rabbit reticulocyte lysate (RRL) (Promega) following the manufacturer’s protocol for non-radioactive luciferase reactions ...
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bioRxiv - Cell Biology 2022Quote: ... Constructs for in vitro translation reactions were based on the SP64 vector (Promega, USA). Templates for transcription were generated by PCR ...
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bioRxiv - Cell Biology 2023Quote: ... was synthesized in vitro using the TNT-coupled transcription-translation system (Promega, Madison, WI) using DNA templates pcDNA3.1– 2xHA-PXF-1 and pcDNA3.1–2xHA-PXF-1(K191/P195/P198->AAA) ...
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bioRxiv - Immunology 2023Quote: ... Unpurified PCR product was used as input to rabbit reticulocyte transcription translation system (Promega) and Nano-Glo HiBit Lytic Detection System (Promega Cat No ...
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bioRxiv - Microbiology 2023Quote: ... 2.5 μl of translation reaction products and 50 μl of Luciferase Assay Reagent (Promega) were mixed by gently pipetting and subjected to luminometer reading (Cytation 5 imaging multimode reader ...
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The Ets protein Pointed P1 represses Asense expression in type II neuroblasts by activating TaillessbioRxiv - Developmental Biology 2021Quote: ... Proteins were expressed from the pcDNA™3.1/His construct by the TNT® T7 Quick Coupled Transcription/Translation kit (Catalog #L1170, Promega Co., Madison, WI) according to manufacturer’s instructions ...
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bioRxiv - Neuroscience 2022Quote: ... Protein bands were detected with horseradish peroxidase (HRP)-conjugated rabbit or mouse secondary antibody [1:1,000 dilution] (Promega). The chemiluminescence signal was imaged using an Odyssey XF Imager (LI-COR ...
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bioRxiv - Molecular Biology 2021Quote: ... Human SMARCB1 was translated in vitro using TNT Quick Coupled Transcription/Translation System (L1170, Promega). 1 µg of pcDNA3.1-FLAG-SMARCB1 was incubated at 30°C for 90 minutes with 20 µM methionine and TNT T7 Quick Master Mix ...
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Membrane integration and topology of RIFIN and STEVOR proteins of the Plasmodium falciparum parasitebioRxiv - Biochemistry 2019Quote: ... Plasmid pGEM1 and TNT SP6 Quick Coupled Transcription/Translation System were purchased from Promega (USA). All enzymes were from ThermoFisher Scientific (USA ...
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bioRxiv - Cancer Biology 2020Quote: In vitro translation assay for cMYC was performed by rabbit reticulocyte lysate system (Promega, USA) according to manufacturer’s instructions ...
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bioRxiv - Cell Biology 2019Quote: In vitro translation was performed in nuclease-treated rabbit reticulocyte lysates (RRL) (Promega cat #L4960) using a luciferase mRNAs in the presence of the indicated concentrations of (PR)20 ...
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bioRxiv - Cancer Biology 2020Quote: ... In vitro transcription and translation experiments were done with rabbit reticulocyte lysate (TNT systems, Promega) according to the manufacturer’s recommendation ...
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bioRxiv - Biochemistry 2020Quote: ... Radiolabelled PDIP38 preprotein was synthesized using TNT® SP6 Quick Coupled Transcription-Translation System (Promega) with undigested pOTB7/PDIP38 as template and 11 μCi of [35S]Met/CysEXPRE35S35S protein labelling mix (specific activity of >1000 Ci/mmol ...
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bioRxiv - Biochemistry 2022Quote: ... In-vitro translations were done with rabbit reticulocyte lysate (Flexi Rabbit Reticulocyte Lysate System, Promega) and translated CFTR (domains ...