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Citations for Promega :
101 - 150 of 634 citations for IL 6 Rat HEK293 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2021Quote: ... FuGENE® 6 Transfection Reagent (Promega); Luminata Forte Western HRP substrate (Millipore) ...
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bioRxiv - Microbiology 2019Quote: ... using Fugene 6 transfection reagent (Promega) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2020Quote: ... MDCK) or with FuGENE 6 (ProMega) (HeLa) ...
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bioRxiv - Cancer Biology 2021Quote: ... using FuGENE 6 Transfection Reagent (Promega) according to manufacturer protocol ...
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bioRxiv - Genomics 2023Quote: ... and transfected (Fugene 6, Promega # E269A) with pBABE-puro SV40 LT plasmid (Addgene # 13970 ...
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bioRxiv - Immunology 2023Quote: ... using Fugene 6 transfection reagent (Promega) according to the manufacturer’s instructions ...
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bioRxiv - Bioengineering 2020Quote: ... Unstimulated media (+CTRL) contained complete α-MEM medium only and stimulated media (+IL-1β) contained 20 ng/mL IL-1β (FHC05510, Promega, Madison, WI, USA) in complete α-MEM medium ...
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bioRxiv - Biochemistry 2022Quote: ... HEK293 cells were co-transfected with FLAG-tagged receptor constructs and luciferase-based 22F cAMP biosensor (Promega, Cat. no. E2301) using polyethylenimine (PEI ...
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bioRxiv - Cell Biology 2019Quote: Approximately 5 × 107 control cells and 2 × 107 sort3 PIGS-KO HEK293 cells were used for genomic DNA extraction by Wizard Genomic DNA Purification Kit (Promega). Approximately 325 µg of genomic DNA from control cells and 30 µg of genomic DNA from sort3 cells were used for amplification of gRNA ...
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bioRxiv - Molecular Biology 2019Quote: ... Virus-like particles (VLPs) containing the GFP control or targeting sgRNAs were generated by transfection of HEK293 cells with FuGene (Promega). VLPs were collected from culture supernatant ...
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bioRxiv - Immunology 2020Quote: ... Recombinant DJ-1 protein (1 μM) was added to the transfected HEK293 cell lines and luciferase activities were measured using a Luciferase Assay System (Promega). 10 μg mL−1 of peptidoglycan (Sigma-Aldrich) ...
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bioRxiv - Cell Biology 2022Quote: ... 3 μg of psPAX2 packing plasmid and 2 μg of pM2G envelope plasmid were transfected into HEK293 Lenti-X cells using 30 μL of Fugene-HD (Promega) in antibiotic-free DMEM ...
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bioRxiv - Immunology 2022Quote: ... 5×106 HEK293 cells were plated in 15cm petri dishes and transfected the day after with 50 μL FuGENE (E2311, Promega), 10 μg CAR-encoding vectors and 3.3 μg of each 3rd generation lentivirus helper vectors (CART-027CL ...
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bioRxiv - Immunology 2022Quote: ... 5×106 HEK293 cells were plated in 15 cm petri dishes and transfected the day after with 50 μL FuGENE (E2311, Promega), 10 μg CAR-encoding vectors and 3.3 μg of each 3rd generation lentivirus helper vectors (CART-027CL ...
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bioRxiv - Molecular Biology 2024Quote: ... The -1 PRF efficiency was assayed in cultured HEK293 as described previously (Kelly et al. 2020) using dual-luciferase reporter assay system kit (Promega). 24 hr post transfection ...
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bioRxiv - Immunology 2022Quote: ... HEK293 cells were transfected with the ORF8 plasmid together with the transposase coding plasmid (1/10) with Fugene HD (Promega, Germany) and after one day selected with 3 µg/ml puromycin for three days ...
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bioRxiv - Neuroscience 2021Quote: Luminescence assays to monitor cAMP levels were performed as we described previously (Siuda et al., 2015). Briefly, HEK293 cells (ATCC, cat. # CRL-1573) were co-transfected with PPO-Venus and pGloSensor-22F (Promega E2301) plasmids using JetPrime reagent (Polyplus ...
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bioRxiv - Molecular Biology 2023Quote: ... NLS-NSD2-mVenus WT and T1150A plasmids were transfected into 70 % confluent HEK293 SETD2 knockout cells using FuGENE® HD Transfection Reagent (Promega). NLS-mVenus empty vector was used as a negative control ...
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bioRxiv - Molecular Biology 2023Quote: ... a mixture of the 3 Cas9-sgRNA plasmids (150ng/μl) was transfected into HEK293 cells at 70% confluency in fresh medium using FuGENE® HD Transfection Reagent (Promega). Two days after transfection ...
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bioRxiv - Cell Biology 2024Quote: ... hiPSC-aCM or HEK293 cells were incubated with a labeling solution containing both cell-impermeant HTL-Alexa488 (Promega G1001, 1 µM) and Cellmask Deep Red Plasma Membrane stain (Invitrogen C10046 ...
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bioRxiv - Cell Biology 2021Quote: ... Donor 293T cells were co-transfected with 1.5 µg of plasmids encoding different untagged S mutants and 0.6 µg of pmCherry-N1 using 6 µL of Fugene 6 following the manufacturer’s instructions (Promega). Acceptor Vero cells expressing human ACE246 ...
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bioRxiv - Microbiology 2020Quote: ... Donor cells were co-transfected with 1.5 μg pCAGGS-S and 0,5 μg pmCherry-N1 using 6 μl of Fugene 6 following the manufacturer’s instructions (Promega). Acceptor cells were treated with CellTracker™ Green CMFDA (5-chloromethylfluorescein diacetate ...
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bioRxiv - Cell Biology 2020Quote: ... with Fugene 6 transfection reagent (Promega E2691). 10 mM sodium butyrate was then added to the transfected cells 3 hours post transfection for an overnight incubation at 37°C ...
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bioRxiv - Molecular Biology 2020Quote: ... Transfection was performed with Fugene 6 (Promega). Supernatant was filtered at 0.45 µm before storage at −80°C For stable expression of TRIM21 ...
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bioRxiv - Molecular Biology 2021Quote: ... and pCIG2 UBE3AT485A using Fugene 6 (Promega). 4 hours post transfection cells were treated with chemical libraries ...
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bioRxiv - Cell Biology 2019Quote: ... polyethylenimine “MAX” (polysciences) or Fugene 6 (Promega) were used ...
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bioRxiv - Cancer Biology 2019Quote: ... FuGENE 6 (Promega, E2691, Madison, WI, USA) was used ...
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bioRxiv - Molecular Biology 2022Quote: ... or FuGENE® 6 (Promega Cat# E2961) as transfection reagents ...
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bioRxiv - Synthetic Biology 2021Quote: ... Fugene 6 transfection reagent (Promega, WI, USA) was used for transient transfection according to the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2020Quote: ... using FuGene 6 (Promega, catalog no. E2691). 24 hrs post-transfection ...
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bioRxiv - Biochemistry 2021Quote: ... Promega) and 6 µM ATP (UltraPure, Promega) in the kinase reaction buffer (40 mM Tris-HCl pH 7.5 ...
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bioRxiv - Genetics 2020Quote: ... by transient transfection with FuGENE 6 (Promega). On day 1 ...
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bioRxiv - Biochemistry 2022Quote: ... and transiently transfected with Fugene 6 (Promega), following the manufacturer’s instructions ...
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HIVtat Alters Epithelial Differentiation State and Increases HPV16 Infectivity in Oral KeratinocytesbioRxiv - Microbiology 2023Quote: ... Cells were transfected with Fugene-6 (Promega) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... Cells were transfected with Fugene-6 (Promega) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2023Quote: Fugene 6 was obtained from Promega (E2691). Lipofectamine 2000 was obtained from Thermo-Fisher Scientific (11668019) ...
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bioRxiv - Immunology 2023Quote: ... and lentivectors using Fugene 6 (Promega, E2691). 24 hours after transfection ...
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bioRxiv - Cancer Biology 2023Quote: ... FuGENE 6 transfection reagent (Promega, USA #E2691) diluted in serum-free DMEM was added to the plasmid mixture at 1.5:1 (reagent ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... and 4 μl of FuGENE 6 (Promega) in 100 μl of OPTI-DMEM ...
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bioRxiv - Biochemistry 2024Quote: ... Transfections were performed with Fugene 6 (Promega) per manufacturer’s directions for 24-48 hours before selection with 400 μg/mL hygromycin B.
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bioRxiv - Biophysics 2023Quote: ... and FuGENE 6 transfection reagent (Promega, USA), propagated ...
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bioRxiv - Neuroscience 2023Quote: ... using Fugene 6 Transfection Reagent kit (Promega) according to manufacturers’ instructions ...
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Low basal expression and slow induction of IFITM3 puts immune cells at risk of influenza A infectionbioRxiv - Immunology 2019Quote: HEK293 IFITM3−/− cells were plated in a 6-well dish for 24 hours prior to transfection of IFITM3-pcDNA3.1 plasmid DNA using FuGene 6 reagent (Promega) at a ratio of 3:1 ...
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bioRxiv - Biochemistry 2021Quote: ... HEK293 cells were transfected with a PPRE-firefly luciferase reporter plasmid together with phRL-TK Renilla luciferase control vector (Promega, Madison, WI), pCMX-PPARα ...
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bioRxiv - Genomics 2020Quote: ... 500 cells were plated in each well of 96-well-plate and each sample had 6 replicates and monitored for 6 days from day 0 to day 5 by CellTiter-Glo 2.0 Assay (Promega, G9242) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2019Quote: ... purified by ethanol precipitation and co-transfected into Huh7 cells in 6-well plates (90 ng reporter, 10 ng pcDNA-Blast) using FuGENE 6 transfection reagent (Promega). Cells were selected with blasticidin (0.2 μg/mL ...
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bioRxiv - Neuroscience 2019Quote: ... in wells of a 6-well plate and transfected 24 hours later with 500-800 ng plasmid DNA using FUGENE 6 (Promega) according to the manufacturer’s instructions ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... were seeded in a 6-well plate and transfected with 750 ng of each of the AT1R-TANGO plasmids using FUGENE 6 Transfection (PROMEGA). The following day ...
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bioRxiv - Immunology 2020Quote: ... Plasmid transfections of IFITM1 3’ UTR reporter plasmids (500 ng per single well of a 6-well plate) were performed using the FuGENE 6 (Promega), according to manufacturer’s instructions.
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bioRxiv - Cell Biology 2023Quote: ... 1.5 μg of total DNA (1:10 ratio of the Cre plasmid to the fusion protein plasmid) were mixed with 6 μl of Fugene 6 transfection reagent (Promega) in 100 μl of Opti-MEM ...