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Citations for Promega :
101 - 150 of 1599 citations for Human ACE2 19 740 Recombinant Protein Fc Tag CHO since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2023Quote: ... 1U/μL Recombinant RNase inhibitor (Promega, PAN2515), and 0.1% Triton X-100) ...
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bioRxiv - Cell Biology 2021Quote: ... The Halo-Tag or NanoLuc genes were acquired from Promega and were genetically fused to the respective N- or C-termini.
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bioRxiv - Biochemistry 2023Quote: Halo tags were stained with HaloTag® Ligands TMRDirect (Promega), and SNAP tags were stained with SNAP-Cell® 505-Star (NEB) ...
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bioRxiv - Biochemistry 2020Quote: ... recombinant Trypsin was purchased from Promega (WI. USA), Indium-tin-oxide (ITO ...
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bioRxiv - Molecular Biology 2019Quote: ... and 30 μL Recombinant RNasin Ribonuclease Inhibitor (Promega). Ten milligrams of protein was incubated with 4 μL α-myc antibody (Sigma M4439 ...
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bioRxiv - Immunology 2021Quote: ... in the presence of recombinant RNase inhibitor (Promega) and concentration was determined (Nanodrop 2000 spectrometer ...
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bioRxiv - Plant Biology 2024Quote: ... 10μl Recombinant RNasein© Ribonuclease inhibitor (N2515; Promega) was added to inhibit RNaseA activity and samples were flash frozen in liquid nitrogen until analyzed ...
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bioRxiv - Molecular Biology 2024Quote: ... RNase inhibitor (Recombinant RNasin 20 U/ml; Promega) and 0.15 % Tween-20 ...
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bioRxiv - Biochemistry 2023Quote: OuantiLum® Recombinant Luciferase was purchased from Promega. Creatin Kinase from rabbit muscle was purchased from Sigma Aldrich.
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bioRxiv - Biophysics 2023Quote: ... we added 1.5µL of recombinant RNAsin (Promega N2515) and 6µL of nuclease-free water (Promega P1193 ...
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bioRxiv - Biochemistry 2023Quote: ... 0.5 U/μl Recombinant RNasin Ribonuclease Inhibitor (Promega), 45 mM sodium chloride ...
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bioRxiv - Molecular Biology 2024Quote: RNasin 40 U/µL (Promega, Cat#N2511, recombinant)
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bioRxiv - Plant Biology 2020Quote: ... The recombinant Halo-His-gene was expressed using a 30 μL TNT®SP6 High-Yield Wheat Germ Protein Expression System (Promega, USA, Catalog No L3261). Expression levels were determined by SDS-PAGE and western blot ...
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bioRxiv - Neuroscience 2022Quote: Genomic DNA of ARPE-19 cell lines was extracted using the Wizard SV Genomic DNA Purification System (Promega), following manufacturer’s instructions ...
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bioRxiv - Plant Biology 2020Quote: ... Magne® Halo Tag® Beads (Promega, USA, Catalog No G728A) were used to purify recombinant proteins according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2021Quote: ... JF-549 or Halo-tag antibody (1:500, Promega, mouse monoclonal) and measuring the newly synthesized actin (in most cases ...
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bioRxiv - Biochemistry 2020Quote: ... The plasmid expressing the Halo tag (pFN21A) was purchased from Promega. The expression plasmids His12_PreScission _C1_C2B_MUN_C2C_tev_Halo and His12_PreScission _C1_C2B_MUN_tev_Halo (ΔC2C Munc13 ...
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bioRxiv - Biophysics 2021Quote: ... reference beads followed by Halo-Tag amine (O4) ligand (Promega, P6741). The chambers were then treated with a blocking buffer (20 mM Tris-HCl ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... The NanoLuciferase (NLuc) and Halo-Tag genes were obtained from Promega and the plasmids expressing b2AR- ...
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bioRxiv - Immunology 2020Quote: ... the luciferase activities of infected HEK293T/ACE2 cells were detected by the Bright-Luciferase Reporter Assay System (Promega, E2650). The half-maximal inhibitory concentrations (IC50 ...
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bioRxiv - Immunology 2021Quote: ... the luciferase activities of infected HEK293T/ACE2 cells were detected by the Bright-Luciferase Reporter Assay System (Promega, E2650). The IC50 of the evaluated mAbs was tested by the Varioskan LUX Microplate Spectrophotometer (Thermo Fisher) ...
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bioRxiv - Microbiology 2022Quote: ... either with SARS-CoV-2 S and Jun or with ACE2 and Fos plus the pRL Renilla Luciferase (Promega) to normalize the signal ...
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bioRxiv - Microbiology 2024Quote: ... 4 μg TMPRSS2-Flag-N or its point mutations’ encoding plasmids described above were transfected alone or in combination with 4μg ACE2-Flag into 293T cells using FuGene transfection reagent (Promega). Cells were infected with HCoV-HKU1 ...
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bioRxiv - Biophysics 2021Quote: ... DNA constructs of full-length wt and T654A EGFR (1 μg) were transiently transfected into CHO-K1 cells using FuGENE HD Transfection Reagent (Promega, Madison, WI). For single-molecule measurements ...
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bioRxiv - Neuroscience 2021Quote: ... after which D-luciferin-specific Quantilum Recombinant Luciferase (Promega) was added to a final concentration of 25 μg/mL ...
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bioRxiv - Plant Biology 2019Quote: ... and 20 units of recombinant Rnasin RNase inhibitor (Promega) in a final volume of 20 µl ...
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bioRxiv - Biochemistry 2019Quote: We also tested aggregation using Quantilum Recombinant Luciferase (Promega), L-malate dehydrogenase (MDH ...
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bioRxiv - Immunology 2022Quote: ... 200 U/mL Recombinant RNasin® Ribonuclease Inhibitor (Promega), 2 mM DTT) ...
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bioRxiv - Immunology 2022Quote: ... 200 U/ml Recombinant RNasin® Ribonuclease Inhibitor (Promega), 1 tablet cOmplete™ ...
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bioRxiv - Biophysics 2024Quote: ... 80 U of Recombinant RNase inhibitor (Promega, Fitchburg, WI), 8 U of inorganic pyrophosphatase (Sigma-Aldrich ...
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bioRxiv - Microbiology 2023Quote: ... along with 40 units of Recombinant RNasin (Promega, USA). After confirmation of DNA free preparations using PCR ...
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bioRxiv - Microbiology 2023Quote: ... A total of 5 U of recombinant RNasin (Promega) and 10 U of RQ1 RNase-free DNase (Promega ...
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bioRxiv - Developmental Biology 2023Quote: ... 0.5 µl Recombinant RNasin® Ribonuclease Inhibitor (Promega, N251A), 1 µl Reverse Transcriptase (Promega ...
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bioRxiv - Developmental Biology 2023Quote: ... 0.5 µl Recombinant RNasin® Ribonuclease Inhibitor (Promega, N251A), 1 µl Reverse Transcriptase (Promega ...
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bioRxiv - Biophysics 2024Quote: ... 800 U of Recombinant RNase inhibitor (Promega, Fitchburg, WI), 8 U of inorganic pyrophosphatase (Sigma-Aldrich ...
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bioRxiv - Developmental Biology 2024Quote: ... Total peptide concentration was adjusted to 0.15 µg/µL according to the absorbance at 280 nm against a Mass Spec-Compatible Human Protein Extract/Digest calibration curve (Promega), using the Thermo Nanodrop 1000 ND-1000 spectrophotometer (Thermo Fisher).
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bioRxiv - Microbiology 2022Quote: ... twice and 20 µL of a 1:19 mixture of substrate:buffer from the Nano-Glo Live Cell Assay (Promega) were added to 80 µL of OptiMEM per well ...
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bioRxiv - Molecular Biology 2023Quote: ... containing 300 ng of the recombinant T/F LTR-pGL3 plasmid and 100 ng of recombinant control insert DNA-pTarget plasmid (Promega Corporation, Madison, WI) for the Tat minus transfection or 100 ng of recombinant HIV-1C consensus or autologous Tat-pTarget plasmid (obtained from the same sample from which the LTR was derived ...
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bioRxiv - Developmental Biology 2021Quote: ... The GST tag was removed from hTNPO1 using proTEV Plus Protease (Promega), and the protease enzyme was further removed from hTNPO1 protein by Ni-NTA Magnetic Beads (NEB ...
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bioRxiv - Cell Biology 2022Quote: ... Cells were stained with 0.5 μM Halo-tag substrate CA-AlexaFluor488 (Promega) for 30 minutes at 37°C ...
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bioRxiv - Cell Biology 2019Quote: ... Halo-tag expressing cells were labelled with Halo-TMR ligand (Promega - G8251) following the manufacturers instructions for live cell labelling prior to fixation and permeabilization.
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bioRxiv - Biophysics 2022Quote: ... and a goat anti-mouse tag: HRP 0.1 μL/mL (W402B - Promega).
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bioRxiv - Biochemistry 2023Quote: ... and cells were chased with HaLo-Tag TMR ligand (5 µM, Promega) in Opti-MEM for 15 min ...
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bioRxiv - Cancer Biology 2021Quote: ... 19.5µl of nuclease-free water) (FC-121-1030, Illumina; G9441, Promega). Transposition reactions were incubated at 37°C for 30 minutes in an Eppendorf ThermoMixer with agitation at 1000 RPM ...
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bioRxiv - Cell Biology 2021Quote: ... Fresh batches of ARPE-19 cells from a contamination-free stock that had been tested for mycoplasma (MycoAlert; Promega Inc.) were used every 6 to 8 weeks ...
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bioRxiv - Biophysics 2019Quote: Southern blotting was performed in accordance Koch et al.19 Genomic DNA was prepared using the Wizard Genomic DNA Purification kit (Promega) and digested with SspI-HF and MfeI-HF (NEB) ...
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bioRxiv - Cell Biology 2024Quote: pSBtet-BP plasmids encoding various AAK1 WT and AAK1 mutant constructs alongside pCMV(CAT)T7-SB100 were co-transfected into ARPE-19 cells using FuGene HD transfection reagent (Promega), as per manufacturer’s protocol (Promega ...
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bioRxiv - Immunology 2020Quote: ... were pre-coated with recombinant luciferase (0.4μg/ml, Promega, #E1701) or spike protein (2μg/ml ...
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bioRxiv - Microbiology 2023Quote: ... Recombinant plasmids were confirmed by PCR (GoTaq DNA Polymerase, Promega) and Sanger sequencing (Azenta) ...
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bioRxiv - Neuroscience 2020Quote: ... and inserting epitope tags via polymerase chain reaction with Pfu DNA polymerase (Promega). Primers for HA insertion into pIRES2- EGFP-FL ...