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Citations for Promega :
101 - 150 of 5053 citations for C Reactive Protein ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2021Quote: ... 30 min at 60°C) an alkylation (IAA 0.5M, 30 min RT) microtubule-associated protein enriched fractions were digested using trypsin (Gold, Promega, 1 μg / sample, overnight at 30°C). Peptide clean-up was done using OMIX C18 (Agilent ...
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bioRxiv - Cell Biology 2020Quote: ... The protein concentration was estimated using a BCA kit (Promega). The blots were blocked using 5% NFDM (Santa Cruz ...
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bioRxiv - Cancer Biology 2023Quote: ... Protein concentration was determined using Bradford Assay Kit (Promega, USA) and equal amounts of proteins were denatured ...
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bioRxiv - Biophysics 2019Quote: ... Amino-PEG-QDs (Thermo, Q21521MP) were labelled with HaloTag ligand by reaction with N-hydroxysulfosuccinimide reactive Halo-Tag ligand (Promega, P6751) or Snap-Tag ligand for 30 min at room temperature ...
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bioRxiv - Genetics 2021Quote: ... protein samples were digested overnight at 37°C with 125 ng modified porcine trypsin (Trypsin Gold, Promega). Peptides were extracted under acidic conditions and dried out using a speedvac concentrator ...
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bioRxiv - Microbiology 2021Quote: Peptides from cell-surface/biofilm-ECM-associated proteins were released via a Trypsin/Lys-C mix (Promega) at a final concentration of 50 ng µL-1 ...
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bioRxiv - Biochemistry 2019Quote: ... Protein was digested by incubation overnight at 37°C with trypsin (5 ng/ ml, Promega, Madison, WI) in 50 mM NH4HCO3 (pH 7.8) ...
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bioRxiv - Cancer Biology 2022Quote: ... Proteins were digested in-gel using trypsin (Gold Promega, 1 μg per band, overnight at 30°C,) as previously described (Thouvenot et al. ...
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bioRxiv - Cell Biology 2022Quote: ... SDS was removed and proteins were first digested with Lys-C (Wako) and subsequently with Trypsin (Promega) with an enzyme to protein ratio (1:50) ...
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bioRxiv - Neuroscience 2020Quote: ... Protein digestion was performed overnight at 37 °C in a shaking incubator using 0.12 μg trypsin (Promega) at an enzyme to protein ratio of 1:50 (protein content was calculated using independent samples) ...
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bioRxiv - Systems Biology 2019Quote: ... Proteins were digested on-filter (16 hr, 37 °C) with sequencing-grade modified trypsin (50:1, Promega). Proteolysis was terminated and peptides eluted with 20 μL of 10% trifluoroacetic acid followed by centrifugation (13,000 ×g ...
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bioRxiv - Molecular Biology 2020Quote: ... protein samples were treated with Endoprotease Lys-C (Wako) and Trypsin (Trypsin Gold Mass Spec Grade; Promega). Peptide samples were desalted by OMIX C18 pipette tips (Agilent Technologies ...
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bioRxiv - Cell Biology 2020Quote: ... bound proteins were alkylated and digested with endopeptidase Lys-C (Wako) for 3 hours and trypsin (Promega) on beads overnight at 37°C.
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bioRxiv - Cell Biology 2023Quote: ... Proteins were digested in-gel overnight at 37°C with Sequencing Grade Modified Trypsin (Promega; cat. #V5111) diluted in 200uL of digestion buffer (40 mM ammonium bicarbonate ...
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bioRxiv - Systems Biology 2023Quote: ... then proteins were digested with 1 μg of mass spectrometry grade Trypsin/Lys-C enzyme mix (Promega) in 50 μL of digestion buffer (100 mM ABC ...
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bioRxiv - Microbiology 2023Quote: ... Proteins were digested overnight (21 h) at 37 °C with 0.1 μg/μL trypsin (sequencing grade, Promega) dissolved in 1 mM HCl ...
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bioRxiv - Cell Biology 2024Quote: ... alkylated with 10 mM iodoacetamide and subject to sequential protein digestion with Lys-C and trypsin (Promega). Digestion was halted with formic acid ...
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bioRxiv - Cell Biology 2022Quote: ... Baseline fluorescence was measured in an ELISA reader (Promega) following an incubation of 30 min at 37°C ...
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bioRxiv - Cell Biology 2019Quote: ... Samples were diluted two-fold with 20 mM HEPES pH 8.0 and proteins digested with Lys-C (Promega) at a protease/protein ratio of 1:100 (w/w ...
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bioRxiv - Biochemistry 2022Quote: ... the beads with bound recombinant proteins were blocked for 1 h at 4°C using reticulocyte lysate (Promega). Next ...
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bioRxiv - Molecular Biology 2022Quote: ... the proteins were digested overnight at 37 °C with 6 ng μL-1 of Asp-N (Promega, UK). Peptides were extracted in 2 % v/v formic acid ...
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bioRxiv - Microbiology 2022Quote: ... Proteins on magnetic beads were digested overnight at 37°C with 1μl (0.2 μg/μL) of trypsin (Promega) in a 25-mM NH4HCO3 buffer per sample ...
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bioRxiv - Cell Biology 2020Quote: ... The proteins were then digested overnight at 37°C with 0.2 μg of modified sequencing grade trypsin (Promega) in 50 mM ammonium bicarbonate ...
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bioRxiv - Cell Biology 2021Quote: ... Proteins were digested on the column with 0.25 µg of Trypsin/Lys-C protease mix (Promega; cat. V5071), and peptides eluted for mass spectrometry.
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bioRxiv - Molecular Biology 2023Quote: Reduced and alkylated proteins were prepared by in-gel digesting overnight at 37°C using 100ng trypsin (Promega). The supernatant was dried by vacuum centrifugation and the samples were resuspended in 0.1% trifluoro acetic acid ...
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bioRxiv - Cell Biology 2023Quote: ... Proteins were digested in 100 mM ammonium bicarbonate containing 2 μg of trypsin/Lys-C enzyme mix (Promega) at 37 °C overnight ...
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bioRxiv - Genomics 2023Quote: ... Protein was precipitated by the addition of 200 μl Protein Precipitation Solution (Promega Wizard HMW DNA Extraction Kit A2920). Using a wide bore tip the samples were mixed by drawing up contents from the bottom of the tube and then expelled on the side of the tube 5 times ...
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bioRxiv - Cell Biology 2020Quote: ... Proteins were digested with LysC (Wako) (substrate:enzyme=100) overnight at 37°C and then with sequencing grade trypsin (Promega) (substrate:enzyme=100 ...
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bioRxiv - Biochemistry 2020Quote: ... and proteins were finally subjected to overnight digestion with mass spec grade Trypsin/Lys-C mix (Promega, Madison, WI). Digested proteins were finally desalted using a C18 TopTip (PolyLC ...
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bioRxiv - Molecular Biology 2019Quote: ... and C-terminally truncated VP16 (VP16ΔC) proteins were synthesized using a rabbit TNT quick-coupled transcription/ translation system (Promega) with 35S-labeled methionine ...
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bioRxiv - Cancer Biology 2019Quote: ... and proteins were finally subjected to overnight digestion with mass spec grade Trypsin/Lys-C mix (Promega, Madison, WI). Finally ...
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bioRxiv - Cell Biology 2021Quote: ... A total of 0.2 mg of protein was subjected to overnight digestion with 8.0 µg of mass spec grade Trypsin/Lys-C mix (Promega). Following digestion ...
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bioRxiv - Microbiology 2021Quote: ... 0.025% deoxycholate and 200 µg.mL−1 RNase A at 37°C for 20 min to lyse the cells and Protein Precipitation Solution (Promega) was added ...
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bioRxiv - Cell Biology 2019Quote: ... and proteins were finally subjected to overnight digestion with mass spec grade Trypsin/Lys-C mix (Promega, Madison, WI). The digested proteins were desalted using AssayMap C18 cartridges mounted on a BRAVO liquid handling system (Agilent ...
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bioRxiv - Plant Biology 2019Quote: ... In-gel protein digestion was performed overnight at 37°C with trypsin (Sequencing Grade Modified Trypsin, Promega, Madison, USA) at a final concentration of 0.005 µg/µL ...
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bioRxiv - Cell Biology 2020Quote: ... Alkylation was quenched by the addition of 3 mM DTT and proteins were digested overnight at 37°C with 1 μg trypsin (0.5 μg/μl; Promega). Following digestion ...
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bioRxiv - Cell Biology 2021Quote: ... Alkylation was quenched by the addition of 3 mM DTT and proteins were digested overnight at 37°C with 1 μg trypsin (0.5 μg/μl; Promega). Following digestion ...
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bioRxiv - Genetics 2023Quote: ... proteins were extracted by chloroform-methanol precipitation and digested with 2 μg trypsin/Lys-C mixture (Promega, Madison, WI). Samples were labeled with 10-plex tandem mass tag reagents (ThermoFisher Scientific ...
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bioRxiv - Microbiology 2020Quote: PKA activity (PepTag® Non-Radioactive Protein Kinase Assay kit V5340, Promega) was carried out according to the manufacturer’s instructions ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... HiBit-tagged proteins were detected using the NanoGlo HiBit Blotting kit (Promega) following the manufacturer’s instructions ...
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bioRxiv - Physiology 2019Quote: ... The concentrated urea was diluted 12 times with 50 mM ammonium bicarbonate buffer and proteins were digested for 18 hours at 36°C using trypsin/LysC mixture (Promega) in 1:50 (w/w ...
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bioRxiv - Bioengineering 2020Quote: ... In-solution protein digestion was carried out in a ratio of 1:25 w/w Trypsin/Lys-C (Mass Spectrometry Grade, Promega) to protein overnight at 37°C ...
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bioRxiv - Pathology 2020Quote: ... Sample pH was adjusted to 7.5 with 0.05 M TEAB and proteins were digested overnight at 37 °C with sequencing-grade trypsin (Promega, Madison, WI) in 1:30 (w/w ...
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bioRxiv - Cell Biology 2021Quote: ... Trypsin digestion was carried out overnight at 37°C with 1:50-1:100 enzyme-protein ratio of sequencing grade-modified trypsin (Promega) in 50 mM NH4HCO3 pH 7.5 ...
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bioRxiv - Cell Biology 2022Quote: ... Proteins on beads were digested overnight at 37°C by sequencing grade trypsin (12.5 μg/ml; Promega Madison, Wi, USA) in 20 μl of NH4HCO3 25 mmol/l ...
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bioRxiv - Biochemistry 2022Quote: ... Samples were diluted with ddH2O 1:5 and proteins were digested for 20 h at 37°C using trypsin (Trypsin Gold, Promega) at an enzyme/protein ratio of 1:50 ...
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bioRxiv - Molecular Biology 2020Quote: ... and protein complexes were directly digested on-beads by the addition of 2 μg of a Trypsin/Lys-C mixture (Promega). Peptides were isolated on C18 resin tips according to the manufacturer’s instructions (Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2019Quote: ... Immuno-precipitated proteins were released from the resin by on-beads digestion for 15min at 37°C using 200ng of trypsin (Promega). The supernatants were collected and subjected to conventional in-solution tryptic digestion (overnight at 37°C ...
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bioRxiv - Cell Biology 2021Quote: ... v/v) and dehydration in ACN, proteins were digested overnight at 37 °C with trypsin (1:50, w/w) (V5280, Promega). Peptides were extracted from the gel in 50% ACN/0.1% formic acid ...
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Intrarenal B cells integrate in situ innate and adaptive immunity in human renal allograft rejectionbioRxiv - Immunology 2020Quote: ... Trypsin digestion was carried out overnight at 37 °C with 1:50 - 1:100 enzyme-protein ratio of sequencing grade-modified trypsin (Promega) in 50 mM NH4HCO3 pH 7.5 ...