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Citations for Promega :
101 - 150 of 245 citations for 9 Z 11 E 13 Z OCTADECATRIENOIC ACID since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2023Quote: ... the transfection was performed at 9 DIV using the ProFection® Mammalian Transfection System from Promega (Cat#E1200). At 15 DIV ...
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bioRxiv - Neuroscience 2023Quote: Caspase activation was measured by luminescent assays (Caspase-Glo-3/7, -8 or -9, Promega, Madison, WI, USA), in cells treated for 8h with 50uM Aβ40-E22Q ...
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bioRxiv - Cell Biology 2020Quote: ... and 10 μM methionine-free amino acid mixture (Promega). The Click-iT reaction was performed with the Click-&-Go Protein Reaction Buffer Kit (Click Chemistry Tools ...
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bioRxiv - Systems Biology 2019Quote: ... and 50 μM of each amino acid (Promega, #L446A). T7 RNA polymerase (NEBs ...
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bioRxiv - Cell Biology 2021Quote: ... 14 μM amino acid mix w/o methionine (Promega)) ...
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bioRxiv - Neuroscience 2023Quote: ... and stained in Diamond Nucleic Acid Stain (Promega, H1181). All experiments were performed in 8-12 week old animals ...
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bioRxiv - Genomics 2021Quote: ... RNA concentration was measured in a DeNovix DS-11 spectrophotometer (DeNovix Inc, USA) and 4 μg of RNA were subjected to DNAse treatment (RQ1 - Promega) according manufacturer’s instructions ...
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bioRxiv - Genomics 2021Quote: ... full-length BORIS and CTCF 11 ZF domain were translated in vitro using the TnT Quick Coupled Transcription/Translation System (Promega).
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bioRxiv - Neuroscience 2020Quote: ... Cells were harvested at various time points of differentiation over a period of 11 days and then analyzed using a Dual-Glo luciferase assay kit (Promega) following the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2020Quote: The sequence containing three consecutive perfect matches for hsa-miR-145-5p was excised from pSG5-Luc-3xmiR-145 (11) and sub-cloned in pMirGlo (Promega) to generate pMiRGlo 3xmiR-145.
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bioRxiv - Biochemistry 2022Quote: ... 1.7 μg of attB-hACE2-mNG2-1-10 plasmid or attB-S-mNG2-11 plasmid were added into 5 μL FuGENE 6 transfection reagent (Promega) and OptiMEM (Gibco ...
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bioRxiv - Biochemistry 2023Quote: ... Samples were then digested with trypsin (11 μL of 0.5 μg/μL per sample, resuspended in trypsin resuspension buffer containing 20 mM CaCl2; Promega, #V542A) overnight at 37 °C in a shaker incubator ...
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bioRxiv - Genetics 2023Quote: High molecular weight DNA was isolated from spleen tissue of a single male from each of the 11 imported Nachman wild-derived inbred strains using the Wizard DNA Purification Kit (Promega) or the Monarch HMW DNA kit (NEB ...
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bioRxiv - Cancer Biology 2023Quote: ... pH 11 and the fluorescence was measured at 360 nm (ex) / 450 nm (em) using GloMax Explorer plate reader (Promega). The heparan sulfatase activity was confirmed by our recently described 6-O desulfation assay using GlcNS6S-GlcA-Glc6SNS-IdoA2S-GlcNS6S-IdoA2S-GlcNS6S-GlcA-pNP (2S2-6S4 ...
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bioRxiv - Immunology 2023Quote: A total of 125 ng genomic RVFV clone 13 RNA was either mock treated or incubated with 40 U CIAP (Promega, M1821), 2 U RNaseIII (Ambion ...
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bioRxiv - Microbiology 2021Quote: ... The 1/10 of the sample was kept for injection and the 9/10 were digested with trypsin (Promega) (12.5 ng/µl ...
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bioRxiv - Genetics 2021Quote: ... 5 μL of substrate was added to a final concentration of 100 nM 11S fragment and 0.5X furimazine (Promega, NanoGlo Substrate). Luminescence was measured on a ViewLux HTS reader (PerkinElmer ...
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bioRxiv - Microbiology 2023Quote: ... Nucleic acid was recovered using the Maxwell RSC instrument (Promega). After DNAse treatment and elution ...
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bioRxiv - Molecular Biology 2021Quote: ... was previously constructed by inserting a 351 bp-TiLV segment 9 open reading frame (ORF) into pGEM T-easy vector (Promega) as reported earlier (Thawornwattana et al ...
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bioRxiv - Cancer Biology 2021Quote: Caspase 3/7 and 9 activities were assessed using a fluorescence-based Apo-ONE homogenous caspase 3/7 assay kit (Promega) and luminescence-based caspase-glo 9 assay system (Promega) ...
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bioRxiv - Microbiology 2020Quote: ... The cells were then left at 32°C or moved to 37°C for 9 hours before read on a luminometer using the Dual-Luciferase Reporter Assay Step (Promega) according to manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2021Quote: ... and the pSARP-12R4-9 input library with a 3:1 transfection reagent:DNA ratio using Fugene 6 transfection reagent (Promega #E269A). At 72 hrs ...
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bioRxiv - Biophysics 2020Quote: ... as well as expression construct of interest in a 9:8:1 mass ratio into HEK293T cells using FuGENE HD Transfection Reagent (Promega) per manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2019Quote: ... Stable cell lines were generated by co-transfecting pOG44 with pCDNA5/FRT/TO vectors encoding BRCA1 tBRCT (aa 1620-1862) in a 9:1 ratio using FuGENE HD transfection reagent (E2311, Promega). After 10 days of selection using Blasticidin (15μg/ml ...
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bioRxiv - Cancer Biology 2022Quote: ... CaspaseGlo assays were performed in white-walled 96-well plates to measure the activity of caspase 9 (cat. G8210) or caspase 8 (cat. G8200) (both from Promega) according to manufacturer’s instructions ...
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bioRxiv - Biochemistry 2023Quote: ... for 30 min at 37□C (For NVX-CoV2373, 9 different conditions were used viz., 16 h of trypsin (Promega) digestion ...
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bioRxiv - Biophysics 2022Quote: ... reduced with 5 mM of DTT and alkylated with 11 mM iodoacetamide which was followed by in-gel digestion with trypsin (Promega, Madison, WI), chymotrypsin (Promega ...
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bioRxiv - Cancer Biology 2022Quote: ... to 10.5mL of 2.0 x 10^5 of Hek293 Cells (ATCC CRL1573) in growth media (DMEM + 10% FBS) was added 525uL of a 9:1 ratio of carrier DNA (Promega E4881) to a CLK nLuc construct-CLK1 CLK2 or CLK4 (Promega – NV1131 ...
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bioRxiv - Cell Biology 2023Quote: HeLa FlpIn were co-transfected with pCDNA5 constructs with gene of interest and construct of pOG44 recombinase in a 1:9 ratio with Fugene HD (Promega, E2311) under the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2024Quote: ... iCLIP2 libraries were pre-amplified with 6 PCR cycles using short Solexa primers P5 and P3 (Suppl. Tab. 9) and then size-selected using the ProNex Size-Selective Purification System (Promega, NG2001) in a 1:2.95 (v/v ...
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bioRxiv - Biochemistry 2021Quote: ... A 15-amino acid linker and SmBiT subunit (peptide 86, Promega) were attached to the C-terminus of rat Gβ1 ...
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bioRxiv - Immunology 2022Quote: ... the Maxwell Viral Total Nucleic Acid Purification kit (Promega, Madison, WI) was used to isolate viral RNA (vRNA ...
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bioRxiv - Microbiology 2022Quote: ... Nucleic acid extracts were treated using RQ1 RNAse-free DNAse (Promega) according to manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... a complete standard mixture of amino acids (Promega, Medison, WI, USA) was analyzed by CE-MS to obtain MS and MS/MS spectra of the proteinogenic amino acids ...
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bioRxiv - Bioengineering 2023Quote: ... and subsequently stained with Diamond™ Nucleic Acid Dye (Promega, H1181) for 10 minutes ...
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bioRxiv - Microbiology 2019Quote: ... 1 μg of pCMV71 DNA (plasmid was kindly provided by Mark Stinski, University of Iowa) and 9 μl of ViaFect reagent (Promega, Madison, Wisconsin) were mixed together with 100 μl of MEM without antibiotics ...
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bioRxiv - Cancer Biology 2019Quote: ... These were transfected into Tet-On HEK-293TLDcells [10] by incubating 3 µg plasmid DNA with 9 µL Fugene HD (Promega, Madison, WI) in 100 µL Optimem for 15 min ...
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bioRxiv - Cancer Biology 2021Quote: ... All human cell lines used were authenticated at the Johns Hopkins Genetic Resources Core Facility via short tandem repeat profiling of 9 genomic loci with the GenePrint 10 System (Promega, Madison, WI) before use ...
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bioRxiv - Molecular Biology 2021Quote: ... 2mM Mg(OAc)2 and 10μM of the amino acid mixture (Promega). Reactions were incubated with 5μM of the indicated nsp1 protein for 30 min on ice ...
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bioRxiv - Molecular Biology 2021Quote: ... Plasmids were eluted with RNase free water and used as templates for in vitro gene expression (E. coli S30 Extract System for Circular DNA, Promega).
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bioRxiv - Biochemistry 2020Quote: ... or ∼30 ng (Proevolidine and Proxanthoxycyclin E gDNA) of the A-tailed PCR product were ligated into 50 ng of the pGEM-T Easy vector (Promega) according to the manufacturer’s instructions ...
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bioRxiv - Synthetic Biology 2021Quote: ... Plasmids were resuspended in RNase-free water and used as templates for in vitro gene expression (E. coli S30 Extract System for Circular DNA, Promega). Reactions were set up by adding 1 μL of 0.25 mM amino acid mix ...
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bioRxiv - Cancer Biology 2022Quote: ... PLAT-E cells were transfected with the pMYs STEAP1-mBBζ CAR retroviral construct using the FuGENE HD transfection reagent (Promega). 48 and 72 hours after transfection ...
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bioRxiv - Neuroscience 2021Quote: ... The amplified coding sequence was then subcloned into the pGHJ vector after double digestion with SmaI e EcoRI restriction enzymes (Promega). hTGR5 gene was in pCMV6-Entry (GPBAR1 Human cDNA ORF Clone ...
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bioRxiv - Cancer Biology 2021Quote: ... and MCF-7/CA-IX cell lines by standard clonogenic stable cell construction procedures using Fugene HD (Promega, E 2311). The U2-OS and HEK-293 cells transfected with empty pCMV6 (PS10001 ...
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bioRxiv - Genetics 2022Quote: ... The amplification was performed in 20 μL of reaction (2XPCR FailSafeTM PreMix E from Biosearch Technologies, 1.25 U of GoTaq DNA Polymerase (Promega), < 1μg DNA template and 0.5 μM of each specific primers ...
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bioRxiv - Immunology 2023Quote: ... Retrovirus was generated by transfecting the Plat-E packaging cell line with 10 µg plasmid DNA and 30 µl FuGene HD transfection reagent (Promega). Viral supernatants were collected from transfected Plat-E cells at 40 ...
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bioRxiv - Cell Biology 2022Quote: HeLa cells were transfected with pEGFP-N1 or pEGFP-DFCP1 and co-transfected with pcDNA3-myc-Ubiquitin using Fugene6 (Promega 2023-11-06 Lot 0000425615). 24 h later the cells were washed twice in ice cold PBS and lysed (125 mM KAc ...
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bioRxiv - Plant Biology 2021Quote: The optimised HaloTag®-7 sequence (298 amino acids) from Promega (https://www.promega.de/) was genetically split on position 155/156 aa into the N-terminal fragment “NHalo” (aa 1-155 ...
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bioRxiv - Developmental Biology 2023Quote: ... followed by DNA staining with Diamond™ Nucleic Acid Dye (Promega, Madison, WI)