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Citations for Promega :
1401 - 1450 of 1549 citations for Protein BTG3 BTG3 Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2020Quote: ... and RPM1 CC-HA (1-156aa) were expressed in vitro using the TnT® SP6 High-Yield Wheat Germ Protein Expression System (Promega; Madison, USA) according to the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2020Quote: ... The protein mixture was diluted to 25mg/ml with 50mM ABC and 500ug of protein were digested with 2% w/w Trypsin (Promega, Cat. no. V5111) in 1mL of 50mM ABC overnight at 37°C ...
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bioRxiv - Biochemistry 2021Quote: The kinase activity of the FLT3-ITD protein was measured by using ADP-Glo™ Kinase Assay Kit (Promega, Madison, Wisconsin, the US). Briefly ...
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bioRxiv - Cancer Biology 2022Quote: ... Immunoprecipitated protein–DNA complexes and 5% input were analyzed by qRT-PCR using GO taq QPCR master mix (Promega, A6002, lot no. 0000385100) in triplicate using primers specific for PFKFB3 HREs ...
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bioRxiv - Molecular Biology 2024Quote: ... In vitro transcribed and translated FLAG-tagged ERRα protein was made using the TnT reticulocyte reaction (Promega cat #L1170, 2h on 37°C) or the TnT wheat germ extract (cat #L5030 ...
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bioRxiv - Molecular Biology 2023Quote: ... The resulting proteins with carbamidomethyl groups on their cysteines were digested with 300 ng of sequencing-grade porcine trypsin (Promega, Fitchburg, MA, USA) and injected on an Easy-nanoLC-1000 system coupled to a Q-Exactive+ mass spectrometer (Thermo Fisher Scientific ...
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bioRxiv - Biochemistry 2023Quote: The cell free protein expression assays were performed by using the TnT® Quick Coupled Transcription/Translation System (Cat#L1171, Promega, Madison, USA), according to the manufacturer’s guideline with minor modification ...
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bioRxiv - Biochemistry 2023Quote: ... each sample was hydrolyzed to 100 µg of protein in a 50: 1 ratio using trypsin (trypsin Gold, MassSpec Grade, Promega, Fitchburg, WI, USA). After enzymolysis at 37 for 14 h ...
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bioRxiv - Molecular Biology 2024Quote: ... pH 8.5 to a final urea concentration of 2 M for Trypsin/Lys-C based overnight protein digestion at 37 °C (0.5 µg protease, Mass Spectrometry grade, Promega Corporation, Cat No: V5072.)
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bioRxiv - Plant Biology 2024Quote: ... 25 µg of phloem sap and 50 µg of leaf tissue protein extract were then digested in solution using a Trypsin/Lys-C mixture (Mass Spec Grade, Promega, Madison, WI, USA) according to the instruction manual ...
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bioRxiv - Molecular Biology 2024Quote: ... Seventy micrograms of protein were digested overnight using the filter-aided sample preparation (FASP) method 23 with Trypsin/Lys-C mix (Promega, Madison, WI, USA) (enzyme-to-protein ratio 1:35 ...
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bioRxiv - Molecular Biology 2020Quote: ... followed by a secondary anti-rabbit IgG antibody conjugated to alkaline-phosphatase (Promega, Madison, WI, USA). The membranes were blocked in TBST (150 mM NaCl ...
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bioRxiv - Neuroscience 2021Quote: ... followed by the incubation with a secondary antibody anti-mouse IgG HRP conjugate (W4021, Promega, U.S.A.). All the primary antibodies were diluted 1/3000 in 10% Blocking One/TTBS (50 mM Tris ...
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bioRxiv - Molecular Biology 2021Quote: Primary antibodies and the dilutions used are as follows: anti-Halo (mouse, Promega G9211, 1:200); anti-Ezh2 (mouse ...
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bioRxiv - Cell Biology 2019Quote: The primary antibody was incubated in 1× PBST (PBS + 0.1% Triton X-100) + 0.2% BSA (Promega) overnight at 4°C ...
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bioRxiv - Molecular Biology 2020Quote: ... at a 1:1000 dilution and a secondary antibody against rabbit coupled to HRP (Promega W4011) at a 1:20 000 dilution.
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bioRxiv - Microbiology 2021Quote: ... immunoblots were probed with the appropriate secondary α-mouse antibody conjugated to horseradish peroxidase (Promega, W402B) at a dilution of 1:5000 ...
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bioRxiv - Cell Biology 2020Quote: ... Horseradish peroxidase (HRP)-coupled secondary antibodies directed against mouse or rabbit IgG were purchased from Promega. Chemiluminescence images were acquired using the ImageQuant LAS 500 system (GE Healthcare).
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bioRxiv - Neuroscience 2020Quote: ... were used as secondary antibodies and visualized by detecting chemiluminescence (32209, Pierce) or fluorescence (S1000, Promega) signals ...
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bioRxiv - Bioengineering 2020Quote: ... and detected with anti-rabbit or anti-human HRP-conjugated secondary antibodies (Promega W4011 and W4031). Blots were developed using ECL (ThermoScientific 32106 ...
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bioRxiv - Developmental Biology 2020Quote: ... Primary antibodies used were: rabbit and mouse anti-βGal (1/1000; Promega and MP Biomedicals, respectiveley), rat anti-Ser (1/1000 ...
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bioRxiv - Cancer Biology 2020Quote: ... and then with 1:5000 of anti-rabbit (#W401B) or anti-mouse (#W402B) secondary antibodies (Promega).
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bioRxiv - Cancer Biology 2021Quote: Antibody-dependent cell-mediated cytotoxicity (ADCC) was assessed by an ADCC Reporter Bioassay (Promega, Madison, WI) following the manufacturer’s protocol ...
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bioRxiv - Genetics 2019Quote: ... As secondary antibodies horseradish peroxidase (HRP)-conjugated α-mouse or α-rabbit IgG (Promega, 1:4000) were used ...
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bioRxiv - Molecular Biology 2021Quote: ... Secondary horseradish peroxidase-conjugated anti-mouse or anti-rabbit rabbit IgG antibody (Promega #W4021 and #W4011) were used at 1:2,500 dilution followed by detection by ECL (SuperSignal West Pico Chemiluminescent Substrate ...
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bioRxiv - Cell Biology 2022Quote: ... Subsequently anti-rabbit IgG horseradish peroxidase (HRP)-conjugated secondary antibody (Cat. No. W4011, Promega, Madison, WI) was used to detect the protein of interest with chemiluminescence reaction ...
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bioRxiv - Cell Biology 2022Quote: ... washed and incubated with secondary antibody goat-anti-rabbit-HRP or goat-anti- mouse-HRP (Promega) for 1 h ...
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bioRxiv - Genomics 2022Quote: ... Sections were then incubated with a horseradish peroxidase-labeled anti-rabbit antibody (1:300, Promega, W401B) for 1 hour at room temperature ...
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bioRxiv - Plant Biology 2024Quote: ... the membranes were incubated with an HRP-conjugated secondary antibody (Promega, Cat# W4021, 1:10,000 dilution) in blocking solution ...
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bioRxiv - Animal Behavior and Cognition 2024Quote: ... Horseradish peroxidase (HRP)-coupled secondary antibodies directed against mouse or rabbit IgG were purchased from Promega for chemiluminescence imaging ...
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bioRxiv - Immunology 2023Quote: ... 100 μl of 5,000-fold diluted Peroxidase AffiniPure goat anti-human IgG (H+L) antibody (Promega) was added into each well and incubated for 1 h at 37 °C ...
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bioRxiv - Biochemistry 2023Quote: ... Horseradish peroxidase (HRP)-coupled secondary antibodies directed against mouse or rabbit IgG were purchased from Promega. Chemiluminescence images were acquired using the ImageQuant LAS 500 or 600 system (GE Healthcare).
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bioRxiv - Genetics 2023Quote: ... and for 1 hour at room temperature in secondary antibody (1:4,000 anti-Mouse-HRP, Promega). In order to detect the endogenous Ptiwi09 protein ...
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bioRxiv - Microbiology 2023Quote: ... and then incubated for another hour with an anti-Mouse IgG HRP-conjugate secondary antibody (Promega) (diluted 1:10 000 in PBST) ...
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bioRxiv - Plant Biology 2024Quote: ... the membrane was incubated with a horseradish peroxidase (HPR) conjugated anti-rabbit antibody (Promega, Walldorf, Germany) diluted 1:10.000 in PBS-T with 3% milk powder for 1 h ...
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bioRxiv - Microbiology 2022Quote: ... the gel lane was cut into 10 bands and the proteins were subjected to in-gel digestion with trypsin (Promega, Madison, WI, United States) as previously described (Leijon et al. ...
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bioRxiv - Microbiology 2023Quote: ... Samples were diluted in 50 mM ammonium bicarbonate and normalized to 50 μg protein for overnight LysC/trypsin digestion (Promega, protein:enzyme ratio, 50:1). Trifluoroacetic acid (TFA ...
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bioRxiv - Cell Biology 2023Quote: ... pH 8.5 to a final urea concentration of 2 M for Trypsin/Lys-C based overnight protein digestion at 37 °C (40 µg of protein used for global proteomics and the remainder for phosphoproteomics, 1:70 protease: substrate ratio, Mass Spectrometry grade, Promega Corporation, Cat No: V5072.) Digestions were acidified with trifluoroacetic acid (TFA ...
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bioRxiv - Neuroscience 2021Quote: ... and incubated for 1 h with the secondary antibody anti-mouse IgG HRP conjugate (W4021, Promega, U.S.A.) diluted at 1/3000 in 10% Blocking One/TTBS ...
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bioRxiv - Microbiology 2020Quote: ... the blots were incubated for 1 hour with secondary antibody (goat anti-rabbit IgG-HRP, Promega W4018) that was diluted 1:10000 in blocking buffer ...
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bioRxiv - Plant Biology 2021Quote: ... Dilutions of antibodies applied were: rabbit anti-ferritin 1:10000 (primary) and anti-rabbit HRP conjugated (Promega) 1:10000 (secondary).
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bioRxiv - Neuroscience 2020Quote: ... Tissues were then stained using the following primary antibodies overnight: mouse anti-β Galactosidase (1:1000; Promega), rabbit anti-Er81 (1:5000 ...
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bioRxiv - Cancer Biology 2020Quote: ... The membrane was washed with TBS-T followed by incubation with horseradish peroxidase-conjugated secondary antibody (Promega).
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bioRxiv - Neuroscience 2022Quote: ... Primary antibodies: mouse anti-β-tubulin (E7, Developmental Studies Hybridoma Bank) and rabbit anti-Halo (G9281, Promega). Secondary antibodies ...
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bioRxiv - Cell Biology 2022Quote: ... a sheep anti-BrdU antibody was used in the presence of RQ1 DNase (Promega, 10 units/ml). and after washing with conjugated anti-sheep antibodies (see antibodies section) ...
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bioRxiv - Developmental Biology 2019Quote: ... Immuno-signals were visualized with an alkaline-phosphatase (AP)-conjugated anti-rabbit IgG antibody (1:5000, Promega). Western blot revelation was performed using a cheminoluminescence digital imaging system (ImageQuant Las-4000 Mini ...
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bioRxiv - Molecular Biology 2020Quote: ... The membrane was probed with α-Halotag mouse monoclonal antibody (Promega, diluted 1:1000 in Blocking Buffer), washed 3 times in TBS-Tween ...
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bioRxiv - Molecular Biology 2020Quote: ... then probed with goat α-mouse HRP-conjugated secondary antibody (Promega, diluted 1:10000 in Blocking Buffer) and finally washed 3 times in TBS-Tween ...
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bioRxiv - Biochemistry 2023Quote: ... cells were incubated with a 1:100 dilution of primary mouse anti-NLuc antibody (Promega, cat#7000) in PBS overnight at 4 °C ...
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bioRxiv - Biochemistry 2022Quote: ... the primary antibody was diluted in blocking buffer as follows: Anti-HaloTag (1:1000; Promega Corporation; G9281); anti-PHC1 antibody (1:500 ...