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Citations for Promega :
1401 - 1450 of 5310 citations for 6 methyl 5 4 phenyl 1 3 thiazol 2 yl 2 trifluoromethyl nicotinic acid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2022Quote: ... and luciferase-IRES-ZsGreen using FuGENE 6 Transfection Reagent (Promega). Culture supernatants were exchanged with fresh medium (high glucose DMEM supplemented with 2 mM L-glutamine ...
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bioRxiv - Cell Biology 2019Quote: ... ‘ΔGsix’ HEK 293 cells were transfected using Fugene 6 (Promega) according to manufacturer specifications.
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bioRxiv - Biochemistry 2021Quote: ... proteins were digested with 6 ng/µL Trypsin (Promega, UK) overnight at 37 °C ...
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bioRxiv - Biophysics 2019Quote: ... mixed with 6 μL of Fugene HD transfection reagent (Promega) and incubated for 15 minutes at room temperature ...
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bioRxiv - Biophysics 2021Quote: ... Cells were transfected using the FuGENE 6 Transfection Reagent (Promega) at a ratio of 1 μg DNA/3 μL FuGENE 6 according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2021Quote: ... Cells were transfected with cDNA using FuGene 6 (Promega, E2691) following manufacturer’s guidelines and a ratio of 0.5 µg of plasmid:1.5 µL FuGene 6 (Promega ...
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bioRxiv - Cell Biology 2021Quote: ... 1.5 μl of Fugene 6 transfection reagent (Promega, Madison, WI) and 100 μl of OptiMEM (ThermoFisher Scientific ...
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bioRxiv - Molecular Biology 2020Quote: ... proteins were digested with 6 ng/µL Trypsin (Promega, UK) overnight at 37 řC ...
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bioRxiv - Genetics 2019Quote: ... each well was transfected with 2.1 ul FuGENE 6 (Promega), 600 ng of the mutant SCN5A plasmid and 100 ng of a plasmid expressing Bxb1 recombinase (a gift from Pawel Pelczar,21 Addgene plasmid #51271) ...
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bioRxiv - Microbiology 2022Quote: ... and gL using 2.5 μL/well FuGENE 6 (Promega, USA) in 500 μL/well DMEM with 10% FBS ...
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bioRxiv - Molecular Biology 2022Quote: ... and 6 ng of Renilla luciferase vector (Promega, Cat. E6921) were co-transfected into HEK 293 cells ...
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bioRxiv - Molecular Biology 2022Quote: ... mESCs were transfected using the Fugene 6 Transfection Reagent (Promega), according to the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2023Quote: ... the cells were transfected using FuGENE 6 transfection reagent (Promega). Transfection mixture was prepared in serum-reduced OptiMEM medium (Thermo ...
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bioRxiv - Biochemistry 2023Quote: ... 6 hour digestion (both enzymes from Promega, 200 ng each). Samples were equilibrated to 0.1 % TFA ...
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bioRxiv - Cancer Biology 2023Quote: ... HUH7 cells were transfected with FuGene 6 (Promega, Madison WI), and cell pellets were harvested 40 hours after transfection ...
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bioRxiv - Cell Biology 2023Quote: ... followed by addition of 6 μL of FuGene HD (Promega). This entire mix was dripped onto the culture and incubated without shaking ...
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bioRxiv - Cell Biology 2023Quote: ... 6 µg total RNA was treated with DNase RQ1 (Promega), purified with RNA Clean & Concentrator-5 kit (Zymo Research ...
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bioRxiv - Cell Biology 2023Quote: ... proteins were digested with 6 ng/µL trypsin (Promega, UK) overnight at 37 °C ...
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bioRxiv - Molecular Biology 2024Quote: ... Cells were transfected using FuGENE 6 transfection reagent (Promega, E2691). Briefly ...
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bioRxiv - Immunology 2024Quote: ... along with 6 μM EnduRen™ Live Cell Substrate (Promega). Luminescence was then measured over 12 h using the EnVision Multilabel Reader (Perkin Elmer) ...
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bioRxiv - Immunology 2024Quote: ... replication competent clones into 293T cells with Fugene 6 (Promega). Plasmids encoding full-length Q23-17 (shortened as Q23) ...
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bioRxiv - Molecular Biology 2019Quote: The activity of caspase 3/7 was measured using the Caspase-Glo 3/7 assay kit (Promega) following the instructions ...
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bioRxiv - Biochemistry 2021Quote: The activity of Caspase 3/7 was determined using the Caspase-Glo® 3/7 Assay (Promega). Upon incubation of the cells with the different treatment regimens ...
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bioRxiv - Cancer Biology 2022Quote: ... Cleavage of Caspase 3 and 7 was quantified by using Caspase-Glo 3/7 Assay System (Promega) according to the manufacturer’s instructions.
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bioRxiv - Cell Biology 2021Quote: ... caspase-3/7 activity was determined using the Apo-ONE® homogeneous caspase-3/7 assay (Promega) following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2024Quote: ... Caspase 3/7 activity was then quantified using the Apo-One Homogenous Caspase-3/7 assay (Promega).
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bioRxiv - Cancer Biology 2023Quote: ... Caspase-3 and -7 activities were measured using the Caspase-Glo 3/7 assay kit (Promega, G8090) according to manufacturer’s instructions ...
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bioRxiv - Cell Biology 2023Quote: Caspase 3/7 activity was measured using the Caspase-Glo® 3/7 Assay System (G8090, Promega) per the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2021Quote: ... free thiols were alkylated by iodoacetamide and proteins were sequentially digested with endoproteinase Lys-C (Wako, 1:100 enzyme-to-substrate ratio, 3 h at 37 °C) and trypsin (Promega, 1:50, overnight at 37 °C). Digested samples were desalted ...
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bioRxiv - Developmental Biology 2021Quote: ... Probes are synthetized from a PCR product or a linearized plasmid (see Table 2) with the T7 or SP6 RNA polymerase (Promega, P4074 or P4084) in presence of DIG-UTP nucleotides ...
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bioRxiv - Systems Biology 2019Quote: ... the cells were immediately re-plated in 2 ml of complete medium for 24 hours and then lysates were analyzed with the Dual Luciferase reporter system (Promega Catalog No. E1910).
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bioRxiv - Biochemistry 2019Quote: ... The beads were again washed with 50 mM ammonium bicarbonate buffer and resuspended in 100 µl of fresh 50 mM ammonium bicarbonate buffer and treated with 2 µg of sequencing grade modified trypsin (Promega; Product code V5113) at 37 oC overnight ...
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bioRxiv - Molecular Biology 2020Quote: ... The RNA samples (2 μg) were used for the synthesis of cDNA by reverse transcription using GoScript ™ Reverse Transcriptase (Promega, Madison, USA). RT-PCR was performed with a CFX96 Touch ™ Real-Time PCR Detection System (Bio-Rad Laboratories) ...
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bioRxiv - Cell Biology 2021Quote: Hela cells were transfected with 2 µg of HaloTag® fusion construct and 20 ng NanoLuc® fusion construct using Fugene® HD (Promega) according to manufacturer’s instructions ...
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bioRxiv - Cell Biology 2020Quote: ... 2 x 105 U2OS cells were transfected with 2 µg of sgRNA and Cas9 expressing plasmid using Fugene HD reagent (Promega, Madison, WI, USA) according to the manufacturer’s instruction ...
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bioRxiv - Microbiology 2022Quote: ... this template was generated from the cDNA of SARS-CoV-2 AI/I-004/2020 using the T7 RiboMAX Express Large Scale RNA Production System (Promega, Madison, WI, USA). The primers and probe used in this study were as follows ...
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bioRxiv - Physiology 2019Quote: ... The wounded area and its surrounding area (2 x 2 cm) were immediately dissected out and RNA extraction was conducted using Maxwell® RSC simplyRNA Tissue Kit (AS1340, Promega, Madison WI) following the manufacturer’s protocol ...
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bioRxiv - Biochemistry 2020Quote: ... The protein mixture was diluted to 25mg/ml with 50mM ABC and 500ug of protein were digested with 2% w/w Trypsin (Promega, Cat. no. V5111) in 1mL of 50mM ABC overnight at 37°C ...
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bioRxiv - Biochemistry 2024Quote: ... was synthesised from 2 µg of total RNA using the Moloney Murine Leukemia Virus Reverse Transcriptase (M-MLV RT; Promega, Madison, WI, USA) using random primers and oligo-dT primers (3:1 mol ...
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bioRxiv - Bioengineering 2023Quote: ... this template was generated from the cDNA of SARS-CoV-2 AI/I-004/2020 using the T7 RiboMAX Express Large Scale RNA Production System (Promega, Madison, WI, USA). The primers and probe used to detect the WK-521 strain were as follows ...
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bioRxiv - Cancer Biology 2023Quote: ... viable cells were quantified in a FLUOstar OPTIMA ELISA reader (550/590 nm) about 2 h after CellTiter-Blue staining (Promega, Cat No. G8081). Mean values +/-standard deviations (SD ...
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bioRxiv - Plant Biology 2023Quote: ... and 2 µg of RNA were used for reverse transcription into complementary DNA (cDNA) using M-MLV Reverse Transcriptase (Promega, Madison, WI, USA) according to the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2023Quote: Affinity purified proteins retained on beads were resuspended in 100 µL of NH4HCO3 at 25 mM containing 2 µg of Trypsin/Lys-C mix (Mass Spec Grade, Promega, Madison, WI, USA). Digestion was performed under agitation at 37°C during 4h ...
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bioRxiv - Microbiology 2024Quote: ... Peptide digestion was initiated by adding 25 µL of 50 mM ammonium bicarbonate buffer (pH 8) containing 2 µg trypsin (Sequencing Grade Modified Trypsin, Promega, Cat. no. V5117) directly on top of the column and incubating overnight at 37°C ...
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bioRxiv - Cell Biology 2024Quote: ... beads were equilibrated in 50 mM of ammonium bicarbonate with 2 washes followed by on-bead digestion with trypsin (800 ng; Promega cat. nr. V5280) overnight at 37 °C ...
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bioRxiv - Biochemistry 2021Quote: ... A 15-amino acid linker and SmBiT subunit (peptide 86, Promega) were attached to the C-terminus of rat Gβ1 ...
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bioRxiv - Immunology 2022Quote: ... the Maxwell Viral Total Nucleic Acid Purification kit (Promega, Madison, WI) was used to isolate viral RNA (vRNA ...
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bioRxiv - Microbiology 2022Quote: ... Nucleic acid extracts were treated using RQ1 RNAse-free DNAse (Promega) according to manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... a complete standard mixture of amino acids (Promega, Medison, WI, USA) was analyzed by CE-MS to obtain MS and MS/MS spectra of the proteinogenic amino acids ...
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bioRxiv - Bioengineering 2023Quote: ... and subsequently stained with Diamond™ Nucleic Acid Dye (Promega, H1181) for 10 minutes ...