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Citations for Promega :
1401 - 1450 of 4203 citations for 6 Chloro 1 phenylsulfonyl 1H pyrrolo 2 3 b pyridine since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2023Quote: ... After 3 days the cells were washed with PBS and lysed with Cell Culture Lysis Buffer (Promega cat. #E1531) prior to firefly and Renilla luciferase activity measurements which were performed in triplicate using the Dual-GLO kit (Promega cat ...
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bioRxiv - Cancer Biology 2023Quote: The 3-dimensional (3D) culture studies were performed using MCF-7 cells and low melting point agarose (Promega, Australia). Agarose was suspended in PBS at a concentration of 0.75% (w/v) ...
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bioRxiv - Evolutionary Biology 2023Quote: ... Probe synthesis reactions were performed at 37 °C for 3 h and then were treated with DNase I (Promega) at 37 °C for 20 min ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... CytoTox-OneTM homogeneous membrane integrity assay kit and Caspase-Glo® 3/7 assay system kit were from Promega, WI ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were seeded onto acid-washed coverslips at a density of 3 × 104 cells per coverslip for 24 h before transfection with 1μg of plasmid DNA using 3 μl Fugene HD transfection reagent (Promega). HaloTagged AP-2 σ2 was visualized by adding Janelia Fluor 646-HaloTag ligand (Promega) ...
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bioRxiv - Genomics 2023Quote: ... at 0.5 uM or DMSO (vehicle) followed by measurement of Caspase activity via the Caspase-Glo 3/7 assay (Promega). Results are representative of at least 3 independent experiments ...
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bioRxiv - Molecular Biology 2024Quote: ... RT-PCR was used to amplify the OGDH 3’UTR and sub clone it into pmirGLO vector (Promega, UK). 5 × 10^4 C2C12 cells were seeded into 12 well plates ...
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bioRxiv - Biochemistry 2020Quote: ... 2 μg of plasmid was transfected using Fugene HD (Promega, Madison WI, #E2311). Plasmid DNA was mixed with 8 µL Fugene and 100 µL media and incubated at room temperature for 15 minutes before being added to cells ...
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bioRxiv - Biochemistry 2021Quote: ... Proteins were digested by 2 h Trypsin/Lys-C mix incubation (V5073, Promega) and collected ...
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bioRxiv - Cancer Biology 2021Quote: ... and real-time PCR using GoTaq 2-Step RT-qPCR kit (Promega, A6110). All measurements were normalized against Actin as the internal control using the 2-ΔΔCt method (Figure 1—source data 3 and Figure 3—source data 2) ...
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bioRxiv - Cancer Biology 2021Quote: ... 4 µl of 5x SSIV buffer and 2 µl of DNase (RQ1, Promega) was added ...
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bioRxiv - Immunology 2021Quote: ... Luciferase activity was measured after 2 days using Nano-Glo luciferase substrate (Promega) and plates were read in an Envision 2103 microplate luminometer (PerkinElmer) ...
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bioRxiv - Biochemistry 2020Quote: ... HALO-MultiDsk (2 mg) was incubated with 200 µl of HaloLink resin (Promega) in binding buffer (50 mM Tris–HCl pH 7.5 ...
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bioRxiv - Cell Biology 2019Quote: ... the proteins were digested with 2 μg of trypsin/Lys-C mix (Promega) for 16 h at 37°C ...
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bioRxiv - Plant Biology 2019Quote: ... 12.50 μl of 2× PCR Green master mix (GoTaq DNA polymerase; Promega, USA) and 8.50 μl nuclease free water ...
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bioRxiv - Biochemistry 2020Quote: ... Seven other trypsins were used and are denoted as Trypsin-2 (V5111; Promega), Trypsin-3 (786-254 ...
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bioRxiv - Neuroscience 2020Quote: ... 7.5 μl of 2× SYBR Green PCR Master Mix (Promega, Madison, WI, USA) and sterile milliQ water to a final volume of 15 μl per tube ...
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bioRxiv - Cancer Biology 2020Quote: ... Protein samples were digested by 2 µg sequencing grade trypsin (Promega, Madison, WI) at 37°C with shaking overnight ...
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bioRxiv - Plant Biology 2021Quote: ... 2 mg of total RNA were treated with RQ1 RNase-Free DNase (Promega) and processed with the ImProm-II Reverse Transcription System (Promega ...
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bioRxiv - Microbiology 2021Quote: ... Luciferase activity was measured after 2 days using Nano-Glo luciferase substrate (Promega) and plates were read in an Envision 2103 microplate luminometer (PerkinElmer) ...
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bioRxiv - Immunology 2022Quote: ... Samples were again diluted to 2 M urea and digested with trypsin (Promega) (1/100 ...
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bioRxiv - Microbiology 2019Quote: ... cells were treated with MEK1/2 specific inhibitor U0126 (20 μM final; Promega). Transfections with siRNAs were performed using JetPRIME (Polyplus ...
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bioRxiv - Genetics 2020Quote: ... Cell growth after 4 days was measured using the CellTiterGlo 2 kit (Promega) and a GloMax luminometer (Promega) ...
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bioRxiv - Physiology 2020Quote: ... PCR amplicons were resolved on a 2% agarose gel (Promega Corporation, Madison, USA) pre-stained with Ethidium bromide (0.5 μg/ml ...
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bioRxiv - Immunology 2020Quote: ... and RFP (to 2 µg) with FuGENE HD according to manufacturer’s instructions (Promega). After 24 h cells were treated with DMSO or VbP (10 µM ...
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bioRxiv - Neuroscience 2022Quote: ... 2 μl of isolated genomic DNA was used for the genotyping PCR (Promega) using respective protocols.
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bioRxiv - Molecular Biology 2022Quote: ... and 2 micrograms from each sample were DNAse I treated (Promega, Madison, WI) following manufacturer’s protocol ...
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bioRxiv - Plant Biology 2023Quote: ... 2 μg of extracted RNA was treated with RQ1 RNase-Free DNAse (Promega), then used for cDNA synthesis using SuperScript IV Reverse Transcriptase (Invitrogen) ...
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bioRxiv - Biochemistry 2023Quote: ... 10 µL of 2 ng/µL trypsin/Lys-C Mix (CAT# V5072, Promega) in 0.3% acetic acid was added to a 96-well plate (CAT# 0030128664 ...
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bioRxiv - Immunology 2023Quote: ... cDNA was prepared by reverse transcription (GoTaq 2-Step RT-qPCR System, Promega) and amplified byPCR using SYBR® Green PCR Master Mix and ABI Prism7900HT sequence detection system (Applied Biosystems) ...
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bioRxiv - Cell Biology 2023Quote: ... and resuspended in 50 μl TdT buffer containing 2 mM rGTP (Promega, E603B) and 20 U TdT at 37°C for 15 min for rGTP tailing37 ...
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bioRxiv - Immunology 2023Quote: ... cDNA was prepared by reverse transcription (GoTaq 2-Step RT-qPCR System, Promega) and amplified by PCR using SYBR® Green PCR Master Mix and ABI Prism 7900HT sequence detection system (Applied Biosystems) ...
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bioRxiv - Cell Biology 2019Quote: ... diluted 1/500 in PBS-BSA 1% and 0.4 U.mL-1 RNAsin (Promega). We visualized and quantified 5-FUrd incorporation by using high content imaging device (OPERETTA ...
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bioRxiv - Molecular Biology 2021Quote: ... Mtfp1 and Gnpat 3’UTRs and Tnksbp1 and Gnpat CDS were amplified by PCR from MIN6 cDNA and subcloned into pmirGLO (Promega), downstream the Firefly ORF ...
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bioRxiv - Developmental Biology 2020Quote: ... and ‘TA cloned’ (after addition of 3’ adenine nucleotide overhangs) it into pGEM®-T-Easy plasmid vector (Promega, A1360). The derived plasmid was mutagenised (commercial service ...
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bioRxiv - Neuroscience 2021Quote: ... reverse mutant primer oIMR1437 5′-TCC ACC TAG CCT GCC TGT AC-3′) with 1U GoTaq polymerase (Promega, Madison, USA), 1X green GoTaq buffer ...
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bioRxiv - Molecular Biology 2020Quote: The non-TOP 5′ UTR sequence of ATP5O (5′-CGGGAGAAG-3′) was identified in published nanoCAGE data 90 and inserted between the T7 promoter and Renilla luciferase in psiCHECK2 (Promega) by Gibson Assembly.
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bioRxiv - Genomics 2019Quote: 48 hours after transfection the HEK293T cells were washed 3 times with PBS and lysed in 100μl of Passive Lysis Buffer (Promega, E1910). Firefly luciferase and Renilla luciferase activities were measured in 10μl of HEK293T cell lysate using the Dual-Luciferase Reporter assay system (Promega ...
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bioRxiv - Microbiology 2019Quote: ... The genes of interest along with ∼700 bp of 3’ and 5’ flanking DNA were amplified and cloned into the vector pGEM-T Easy (Promega) to create pGEMlpg0277 ...
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bioRxiv - Neuroscience 2019Quote: ... a second pool was fixed to the first pool using a drop of agarose low melting 3% (Promega, Ref. V2111) in Leibovitz’s L15 medium (Gibco Life Technologies ...
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bioRxiv - Genomics 2019Quote: Genomic DNA was isolated from the tip of tails of 3-week-old mice using Wizard Genomic DNA purification Kit (Promega), amplified by PCR ...
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bioRxiv - Synthetic Biology 2019Quote: ... Backbone and inserts were ligated either for 3 h at RT or overnight at 4°C using T4 ligase (Promega). All vector sequences (Table S5 ...
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bioRxiv - Biochemistry 2019Quote: ... Reactions (3 μL) were incubated for 60 min at 37 °C and quantified with the Nano-glo assay system (Promega).
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bioRxiv - Biochemistry 2021Quote: ... Genomic regions flanking the CRISPR target sites were PCR amplified with designed primers (Supplemental Table 3) using GoTaq DNA polymerase (Promega) and sent for Sanger sequencing to determine the insertion and deletion errors generated by CRISPR-Cas9 system in exon 2 of PARKIN ...
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bioRxiv - Biochemistry 2020Quote: ... protein was modified with an poly(A)25 insertion at the 3’ end of the gene within the pRL-null vector (Promega). The gene encoding the NanoLuc luciferase was transferred from the pNL1.1 vector (Promega ...
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bioRxiv - Biochemistry 2020Quote: ... The membrane was probed with a 400 bp 32P-labelled PCR product of the TbCls 3’UTR generated with the prime-a-gene labelling system (Promega). Primers used for PCR were ...
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bioRxiv - Bioengineering 2020Quote: ... Reverse transcription into cDNA was done from 3 μg of RNA by using 500 μg/mL random hexamers (Promega, Switzerland) and 0.5 μL of 200 UI/mL SuperScript III reverse transcriptase (Invitrogen) ...
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bioRxiv - Neuroscience 2020Quote: Caspase 3/7 activity in primary neuronal cells was measured using ApoTox-Glo Triplex Assay kit (Promega, Madison, WI, USA) as per the manufacture’s instruction ...
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bioRxiv - Cancer Biology 2020Quote: ... The viability of cells was measured after 3 days incubation using a CellTiter-Glo Luminescent Cell Viability Assay kit (Promega) following the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2021Quote: ... To remove unwanted 3’phosphate groups from bound RNA fragments in both CLASH and FLASH the complexes were treated with TSAP phosphatase (Promega) using provided buffer for 40 min at room temperature ...