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Citations for Promega :
1351 - 1400 of 1676 citations for Recombinant Mouse MSTN Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2022Quote: ... IAA was neutralized by adding 10mM DTT final concentration and proteins were digested with 300ng of trypsin (Promega V5280) for 18h ...
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bioRxiv - Cell Biology 2019Quote: ... and proteins were finally subjected to overnight digestion with mass spec grade Trypsin/Lys-C mix (Promega, Madison, WI). The digested proteins were desalted using AssayMap C18 cartridges mounted on a BRAVO liquid handling system (Agilent ...
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bioRxiv - Biochemistry 2020Quote: ... N-glycans were released from the intact proteins using 0.5 U/ µL N-glycosidase F (PNGase F, Elizabethkingia miricola, Promega), overnight ...
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bioRxiv - Cell Biology 2019Quote: ... the protein sample was digested by incubation with sequencing-grade modified trypsin (1/50, w/w; Promega, Madison, Wisconsin) overnight at 37°C ...
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bioRxiv - Biochemistry 2019Quote: ... All samples were further diluted with 20 mM HEPES pH 8.0 to a final urea concentration of 2 M and proteins were digested with 5 μg trypsin (V5111, Promega) (1/100 ...
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bioRxiv - Cancer Biology 2019Quote: ... Indicated amounts of HMGA2 and mutant HMGA2 protein were incubated with 100ng of negatively supercoiled Renilla reporter plasmid (Promega) for 30mins at 37°C in a buffer containing 10mM Tris-HCl ...
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bioRxiv - Plant Biology 2019Quote: ... In-gel protein digestion was performed overnight at 37°C with trypsin (Sequencing Grade Modified Trypsin, Promega, Madison, USA) at a final concentration of 0.005 µg/µL ...
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bioRxiv - Molecular Biology 2020Quote: ... ZmCLA4 fused to HaloTag was expressed using the TnT SP6 High-Yield Wheat Germ Protein Expression System (L3260) (Promega), and then purified using Magne HaloTag Beads (G7281 ...
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bioRxiv - Cell Biology 2020Quote: ... Alkylation was quenched by the addition of 3 mM DTT and proteins were digested overnight at 37°C with 1 μg trypsin (0.5 μg/μl; Promega). Following digestion ...
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bioRxiv - Immunology 2020Quote: ... The following day the cells were transfected with plasmids encoding the indicated FLAG-tagged protein (2 μg) with FuGENE HD according to manufacturer’s instructions (Promega). After 48 h cells were harvested and washed 3 times with PBS ...
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bioRxiv - Cell Biology 2021Quote: ... Alkylation was quenched by the addition of 3 mM DTT and proteins were digested overnight at 37°C with 1 μg trypsin (0.5 μg/μl; Promega). Following digestion ...
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bioRxiv - Genetics 2020Quote: ... Protein extracts were obtained from in vitro coupled transcription/translation systems (TNT® T7 Coupled Reticulocyte Lysate Systems, Promega). 15 µl of Flag-tagged proteins from TNT assays were pre-incubated on ice for 10 minutes in modified DBB (DBB ...
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bioRxiv - Cancer Biology 2020Quote: ... and the protein pellet was resuspended in 50 mM ammonium bicarbonate and digested with sequencing-grade modified trypsin (Promega) overnight at 37°C ...
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bioRxiv - Physiology 2022Quote: ... The proteins were cleaved into tryptic peptides by addition of 25 μl of a 0.2 μg/μl trypsin (Promega) in 50 mM ammonium bicarbonate and incubation (18 h ...
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bioRxiv - Plant Biology 2022Quote: ... ZmEREB57 fused to HaloTag was expressed using the TnT SP6 High-Yield Wheat Germ Protein Expression System (L3260) (Promega), and then purified using Magne HaloTag Beads (G7281 ...
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bioRxiv - Cancer Biology 2024Quote: ... The identity of each cell line was confirmed by DNA fingerprinting via short tandem repeats at the time of mRNA and total protein lysate preparation using the PowerPlex 1.2 kit (Promega).
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bioRxiv - Molecular Biology 2023Quote: ... Bands corresponding to the protein of interest were cut from the gel and in-gel digestion with trypsin (Promega) was performed essentially as described in 41 ...
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bioRxiv - Evolutionary Biology 2022Quote: ... we dissolved 10 μg of protein in 10 μl 10 M urea containing 0.1% ProteasMax (Promega. Madison, WI, USA). Cysteine residues were reduced with 5 mM DTT (30 min at 50 °C ...
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bioRxiv - Systems Biology 2023Quote: ... Samples were diluted 1:3 with 50 mM HEPES pH 8.5 and then digested by a 1:50 (trypsin to protein) ratio of sequencing grade modified trypsin (Promega) for 16 hours at 600 rpm and 25°C ...
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bioRxiv - Molecular Biology 2023Quote: ... Beads were rinsed using 80% EtOH and proteins were digested with 100 µL of trypsin in 50 mM TEAB pH 7.55 (Promega, protein:enzyme ratio 1:50 ...
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bioRxiv - Molecular Biology 2023Quote: Electrophoretic Mobility Shift Assays were performed using proteins expressed in the TNT T7 Coupled Reticulocyte Lysate System (Promega, WI), according to the manufactureŕs recommendation ...
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bioRxiv - Cancer Biology 2023Quote: ... Unlabeled proteins were cell-free-synthesized using the TNT T7 Quick Reaction system according to the manufacturer instructions (Promega) and incubated with purified GST fusion proteins coupled with glutathione sepharose 4B fast flow beads (GE healthcare ...
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bioRxiv - Cell Biology 2023Quote: ... Samples were further diluted with 50 mM Tris pH 7.9 pH 8.0 to a final urea concentration of 2 M and proteins were digested with 5 µg trypsin (Promega) (1/100 ...
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bioRxiv - Molecular Biology 2022Quote: ... 50 ng/well of chaperone RTP1S,105 50 ng/well of the G protein subunit Gαolf,106–107 olfactory G protein subunit Gγ13,108 and 50 ng/well of pGloSensorTM-22F (Promega, Madison, USA).109 The utilized pGloSensor™-22F is a genetically engineered luciferase with a cAMP-binding pocket ...
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bioRxiv - Genetics 2023Quote: ... proteins were extracted by chloroform-methanol precipitation and digested with 2 μg trypsin/Lys-C mixture (Promega, Madison, WI). Samples were labeled with 10-plex tandem mass tag reagents (ThermoFisher Scientific ...
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bioRxiv - Cell Biology 2023Quote: ... urea was diluted to 1 M and proteins digested overnight with modified sequencing grade trypsin (Promega, Madison, WI, USA) at a 1:50 enzyme:protein ratio ...
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bioRxiv - Plant Biology 2024Quote: ... proteins that underwent in vitro reactions were digested in 50 mM ammonium bicarbonate (NH4HCO3) with 0.2 µg trypsin (Promega) at 37°C overnight ...
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bioRxiv - Plant Biology 2024Quote: ... and αKNL2-CΔDNAb (1,2) fused with a FLAG tag were used for protein expression with TNT SP6 High Yield Wheat Germ (Promega) following the manufacture’s protocol ...
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bioRxiv - Microbiology 2024Quote: ... The sample was processed according to the SP3 protocol36 and digested overnight with trypsin (Promega, enzyme/protein 1:50). Peptides were desalted using SBD-RPS tips as previously described37 ...
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bioRxiv - Molecular Biology 2024Quote: ... pH 8.0 to an urea concentration of 4 M and proteins were digested with 40 µg endopeptidase LysC (Promega) (1/250 ...
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bioRxiv - Molecular Biology 2024Quote: ... Samples were further diluted with 20 mM HEPES pH 8.0 to a urea concentration of 2 M and proteins were digested with 50 µg trypsin (Promega) (1/200 ...
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bioRxiv - Immunology 2021Quote: ... followed by three washes with TBS-T and 1 hr incubation in TBS-T + 1% milk + anti-mouse serum (1:10,000) (Promega). All steps were carried out at room temperature ...
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bioRxiv - Developmental Biology 2021Quote: ... and mouse cyclin B1 were generated using the Riboprobe in vitro Transcription Systems kit according to the manufacturer’s protocol (Promega). Anti-BrdU antibodies were conjugated with protein A-sepharose beads overnight at 4C (GE Healthcare) ...
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bioRxiv - Molecular Biology 2020Quote: ... whereas Bmal1-luc was constructed by inserting the mouse Bmal1 promoter (−779 to +127 in reference to the Bmal1 TSS) to pGL4.11[luc2P] (Promega).
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bioRxiv - Molecular Biology 2020Quote: ... Per2E2-luc reporter gene was constructed by inserting the mouse Per2 promoter (−83 to +156 in reference to the Per2 TSS) into pGL4.12[luc2CP] (Promega), whereas Bmal1-luc was constructed by inserting the mouse Bmal1 promoter (−779 to +127 in reference to the Bmal1 TSS ...
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bioRxiv - Cell Biology 2019Quote: ... was amplified from mouse genomic DNA and cloned upstream of luciferase cassette in KpnI/HindIII site of pGL4.23 (Promega) using primers listed in Supplementary table 1 ...
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bioRxiv - Cell Biology 2021Quote: ... membranes were subjected to three wash periods for 5 minutes in 1x TBS with 0.5% Tween and then probed with horseradish peroxidase–conjugated goat anti-mouse or goat anti-rabbit secondary antibodies at 1:2,500 (W4021, W4011, Promega) in 1x TBS with 5% nonfat dry milk and 0.5% Tween at room temperature for 1 hour ...
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bioRxiv - Neuroscience 2022Quote: ... wherein the 3’ UTR of Prr7 (NM_0010302964) was amplified from mouse DNA and cloned into the pmiRGLO dual-luciferase expression vector (Promega). Mutations in miRNA binding sites conserved across mammals were introduced by site-directed mutagenesis using Pfu Plus! DNA Polymerase (Roboklon).
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bioRxiv - Cell Biology 2022Quote: Total amounts of free cholesterol and cholesterol esters were determined in mouse islet lysates as per the manufacturer’s instructions (Promega). Phosphatidylserine levels were measured in mouse islet lysates as per the manufacturer’s instructions (LSBio).
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bioRxiv - Cell Biology 2020Quote: ... Isolated RNA (500ng) was used for cDNA synthesis with mouse Maloney leukemia virus reverse transcriptase (Promega, Madison, WI, USA). SYBR Green RT-qPCR gene expression assays were performed in triplicate in an ABI StepOne Plus thermocycler using appropriate endogenous controls (Applied Biosystems ...
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bioRxiv - Neuroscience 2019Quote: ... Genomic DNA was isolated from tail snip samples with Maxwell® Mouse Tail DNA Purification Kit (Promega, Madison, WI). Polymerase chain reaction (PCR ...
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bioRxiv - Microbiology 2019Quote: ... used at a 1:10,000 dilution and the secondary antibody was an Anti-Mouse IgG HRP conjugate antibody (Promega) also used at a 1:10,000 dilution.
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bioRxiv - Microbiology 2021Quote: ... Samples were then incubated for 1h at room temperature with horseradish peroxidase-conjugated anti-mouse IgG secondary antibody (Promega) at 1:10,000 and peroxidase was detected using the Pierce chemiluminescence system (Pierce ...
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bioRxiv - Neuroscience 2019Quote: ... and mouse Cbln2 E2 fragments were PCR-amplified from genomic DNA of individual species and inserted into pGL4.24 vector (Promega). The sequences of the PCR primers and synthetic oligonucleotides are listed in Supplementary Table 1.
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bioRxiv - Genetics 2019Quote: ... genes were isolated from C57BL/6 mouse genomic DNA by standard PCR and inserted into the pGL4.10 vector (Promega).
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bioRxiv - Neuroscience 2023Quote: ... A 9.5 kb fragment containing upstream promoter region of mouse Frizzled10 (Fzd10) gene was subcloned into pGEM vector (Promega). CreERT2gene (Feil et al. ...
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bioRxiv - Cell Biology 2023Quote: ... Secondary goat anti-mouse and anti-rabbit antibodies conjugated with alkaline phosphatase used for Western blots were from Promega.
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bioRxiv - Microbiology 2023Quote: The potential for M2e-specific antibodies to induce ADCC was evaluated using a mouse FcγRIV ADCC Reporter kit (Promega). In brief ...
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bioRxiv - Molecular Biology 2023Quote: ... The secondary antibody was a horseradish peroxidase polyclonal goat anti-mouse (from 1:5,000 to 1:10,000, depending on the primary antibody; Promega, W4021). Proteins were detected with the ECL chemiluminescence reagent (GE Healthcare ...
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bioRxiv - Neuroscience 2024Quote: ... and mouse HARE5 fragments were amplified from genomic DNA of individual species and inserted into the pNL1.1 vector (Promega) by KpnI and EcoRV enzyme sites ...