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Citations for Promega :
1301 - 1350 of 1911 citations for AKT1 2 3 Rabbit Recombinant mAb since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2020Quote: ... 2 μl of the resulting mRNA was mixed with 1 μl of FluoroTect GreenLys (Promega) and 3 μl of the wheat germ extract and then transferred under 50 μl of the translation buffer (1x SUB-AMIX SGC ...
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bioRxiv - Cancer Biology 2019Quote: ... Luciferase and Renilla activities were measured by Biotek Synergy 2 plate reader (Winooski, VT) using the Dual-luciferase assay kit (Promega, Madison, WI).
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bioRxiv - Microbiology 2021Quote: ... The PCR products were cloned downstream of Renilla luciferase of psiCHECK(TM)-2 vector (Promega) using XhoI and NotI sites.
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bioRxiv - Microbiology 2020Quote: ... cells were transiently transfected with 2 μg DNA using FuGENE® HD (Promega, Madison, WI). Whole-cell patch-clamp recordings were performed at room temperature ...
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bioRxiv - Molecular Biology 2020Quote: Seedlings were sprayed with 2 mM Luciferin (VivoGlo™ Luciferin, In Vivo Grade, Promega, Netherlands) one day before they were released either into continuous light or dark ...
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bioRxiv - Physiology 2022Quote: ... 2-Deoxyglucose-6-phosphate (2DGP) was measured using a Glucose Uptake-Glo Assay Kit (Promega) following the manufacturer’s instructions.
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bioRxiv - Physiology 2022Quote: ... 2-DG uptake was measured using a Glucose Uptake-Glo Assay kit (Promega, Madison, WI).
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bioRxiv - Microbiology 2022Quote: ... Once cooled the beads were digested overnight with 2 µg of Trypsin (Promega Catalog # V5111) at 37 °C and constant rocking.
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bioRxiv - Physiology 2022Quote: ... 2-Deoxyglucose-6-phosphate (2DGP) was measured using a Glucose Uptake-Glo Assay Kit (Promega) following the manufacturer’s instructions.
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bioRxiv - Biophysics 2023Quote: ... cells were transiently transfected with 2 μg DNA by lipofection (FuGENE? HD, Promega, Madison, WI).
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bioRxiv - Molecular Biology 2023Quote: ... Each DNA family was amplified with 2×GoTaq® Hot Start Green Master Mix (Promega) using 0.8–2.2 ng of germline and somatic DNA and the primer pair according to the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2023Quote: ... A volume of 2 µL of the samples was diluted in Glo lysis buffer (Promega) and incubated in a 1:1 ratio for 3 min in the dark with NanoGlo substrate ...
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bioRxiv - Systems Biology 2023Quote: ... 2 mg of total RNA was used for cDNA synthesis with MMLV reverse transcriptase (Promega) in final 50 mL volume ...
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bioRxiv - Cell Biology 2023Quote: cDNA was synthesized from 2 ug of total RNA using GoScript Reverse Transcriptase (Promega, #A5003) with random hexamers according to the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2023Quote: ... The mice received intraperitoneal (IP) injection of 2 milligrams of luciferin (Promega Corporation, Madison, WI) and subjected to luminescence imaging using an IVIS® Spectrum In Vivo Imaging System (PerkinElmer Inc ...
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bioRxiv - Systems Biology 2023Quote: ... and 500 µL per sample were digested using 2 µg Trypsin/LysC (Promega, Cat#V5072). After 17 h of incubation at 37°C ...
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bioRxiv - Systems Biology 2023Quote: ... 2 mg of total RNA was used for cDNA synthesis with MMLV reverse transcriptase (Promega) in final 50 mL volume ...
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bioRxiv - Neuroscience 2023Quote: ... proteins on beads were digested with the addition of 2 µg trypsin (Promega, Cat# V511A) for 18 h at 37°C.
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bioRxiv - Biochemistry 2023Quote: ... Samples were again diluted to 2 M urea and digested with 1 µg trypsin (Promega) (1/100 ...
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bioRxiv - Systems Biology 2023Quote: The transfections were performed with a 1 mg DNA: 2 ml Fugene HD (Promega E2311) ratio ...
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bioRxiv - Cancer Biology 2024Quote: ... Tryptic digest was performed in 50 mM ammonium bicarbonate buffer with 2 μg trypsin (Promega) at 37 °C overnight ...
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bioRxiv - Biochemistry 2024Quote: ... Cells were transfected with 2 µg of plasmids with FuGene HD transfection reagent (Promega #E2312) overnight ...
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bioRxiv - Cell Biology 2024Quote: ... Cells were transfected with 2 μg of bacmid DNA using Fugene HD transfection reagent (Promega) according to the manufacturer’s protocol ...
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bioRxiv - Plant Biology 2020Quote: ... corniculatus plants was synthesized from 3 μg of total RNA using a Moloney Murine Leukemia Virus Reverse Transcriptase (MMLV-RT) (Promega, WI, USA) and 100 pmol of random hexamers (Pharmacia Biotech) ...
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bioRxiv - Cancer Biology 2021Quote: ... DNA:FuGENE complexes were formed at a ratio of 1:3 (μg DNA/μL FuGENE HD) according to the manufacturer’s protocol (Promega, Madison, WI, USA). The resulting transfection complex (1 part ...
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bioRxiv - Microbiology 2019Quote: ... Chimeric pREC NFL INT plasmids were co-transfected into HEK293T cells (3 × 104 cells/well) along with the complementing plasmid pCMV cplt using Fugene 6 reagent (Promega, Madison, WI) as described previously [44] ...
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bioRxiv - Genomics 2021Quote: ... Cells were then grown for 3 days at which point the viability was measured using a CellTiter-Glo assay (Promega Cat# G9241) read out on a standard luminometer ...
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bioRxiv - Biochemistry 2020Quote: ... or PACAP (10 nM final) and assayed 3 hours later with the Nano-Glo® Dual-Luciferase® Reporter System (Cat# N1630; Promega) on a Berthold Sirius luminometer to quantify ERK activation.
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bioRxiv - Biophysics 2020Quote: ... P1KO cells were transiently transfected in a 6-well plate (the cells were seeded 8 h before transfection) in the presence of ruthenium red (10 μM) and mouse Piezo1 (3 μg) using Fugene6 (Promega, Madison, WI). 20–30% of cells showed positive GFP expression indicating successful Piezo1 transfection.
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bioRxiv - Cell Biology 2021Quote: ... Primary antibodies were incubated in PBDS for 3 days at 4°C followed by 4 washes over the next 24 hours with 0.2% Tween-20 (Promega UK Ltd; H5151) in PBS ...
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bioRxiv - Bioengineering 2022Quote: ... The DNA transfection reagents were prepared by incubating 3 µg of plasmid DNA encoding the Bxb1 recombinase and 6 µL of Fugene6 reagent (Promega Cat # E2693) in 300 µL of Opti-MEM for 15 minutes and then added to the cell suspension ...
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bioRxiv - Biochemistry 2022Quote: The 4330bp PCR product amplified from M13mp18 by using primers: oM13-5-27 and oM13-3-24 was inserted into pGEM®-T Easy(PROMEGA #A137A). The resultant plasmid is named as pM13 ...
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bioRxiv - Microbiology 2022Quote: ... PAO1-WT and few colonies that grew in 3 mM 22D-chelated condition were isolated and gDNA extracted using Wizard® Genomic DNA Purification Kit (Promega, USA) for use in whole genome sequencing ...
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bioRxiv - Cell Biology 2022Quote: ... anti-goat alkaline phosphatase-linked antibody and alkaline phosphatase substrate (5-bromo-4-choloro-3-indolyl 1-phosphate and nitroblue tetrazolium) from Promega (Southampton, UK); a hydroxamate-based MMP inhibitor CT-1746 (N1-[2-(S)-(3,3-dimethylbutanamidyl)]-N4-hydroxy-2-(R)-[3-(4-chlorophenyl)-propyl]-succinamide ...
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bioRxiv - Neuroscience 2022Quote: ... and mouse cholecystokinin (CCK, 3-688 of NM_031161) were PCR-amplified and subcloned into the pGEM-T Easy vector (Promega, Fitchburg, WI, USA). Digoxigenin (DIG)-labeled anti-sense probes were prepared using DIG RNA Labeling Mix (Sigma ...
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bioRxiv - Molecular Biology 2020Quote: ... The NanoLuc and Fluc luciferase activities were measured 3 days after transfection using the Nano-Glo® Dual-Luciferase® Reporter Assay System (Promega). The firefly (Fluc ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... RBD-R: 5′-TGC CTC GAG CTC AAG TGT CTG TGGATC AC-3′) into pGEM T-easy vector (Promega, Madison, WI, USA). A standard curve was generated by determining the copy numbers derived from serial dilutions of the plasmid (103–109 copies) ...
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bioRxiv - Microbiology 2020Quote: ... 3’-untranslated region (3’-UTR) fragment of IκBα (human/rat) was inserted into the pGL3-basic vector (firefly luciferase; Promega, Madison, WI, USA), which was obtained from General Biosystems (Anhui ...
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bioRxiv - Cancer Biology 2022Quote: ... we followed the genotyping method for KrasG12D mutation previously described.3 Genomic DNA was isolated from formalin fixed paraffin embedded thymus slides using ReliaPrep FFPE gDNA MiniPrep System (Promega, Madison, WI). The DNA was then amplified using polymerase chain reaction (PCR ...
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bioRxiv - Cancer Biology 2022Quote: Apoptosis induction in response to SRRM1 silencing in leukemia cell lines (25,000 cells/well onto white-walled multiwell luminometer plates) was performed by using Caspase-Glo® 3/7 Assay (Promega Corporation, #G8091) as previously reported (67) ...
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bioRxiv - Microbiology 2019Quote: ... The next day they were transfected over 24 hours with a DNA-Fugene HD mixture at a ratio of 1 μg DNA to 3 μl Fugene (Promega, Southampton, UK) according to the manufacturer’s instructions (Western analysis ...
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bioRxiv - Cancer Biology 2019Quote: ... These were transfected into Tet-On HEK-293TLDcells [10] by incubating 3 µg plasmid DNA with 9 µL Fugene HD (Promega, Madison, WI) in 100 µL Optimem for 15 min ...
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bioRxiv - Biochemistry 2021Quote: The promoter fragment of luciferase reporter plasmid pGADD34-0.5k generated by Eurofins Japan (Tokyo, Japan) was subcloned into a pGL-3 vector (Promega, Madison, WI, USA) by restriction enzyme cutting site KpnI/HindIII ...
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bioRxiv - Molecular Biology 2021Quote: ... A total of 24 clones (4 holobionts x 3 replicates x 24 clones = three 96-well plates) from each amplicon were amplified directly with GoTaq polymerase (Promega, Lyon France) in a 25 μL reaction volume using the following PCR parameters ...
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bioRxiv - Cell Biology 2022Quote: ... Control and treated cells were cultured for 1 day in 96-well plates then treated with small molecules for 3 consecutive days before incubation with 120 μL fresh media containing 20 μL of CellTiter 96® AQueous One Solution (Promega, G3580) for 1 to 4 hours at 37°C in a humidified ...
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bioRxiv - Molecular Biology 2022Quote: ... SEA (0.2 mg/ml) and soluble schistosomula antigens (0.2 mg/ml) was determined using the Caspase-Glo® 3/7 Assay System (Promega, Sydney, Australia) according to the manufacturers’ instructions.
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bioRxiv - Neuroscience 2023Quote: ... Cells were transfected with 1 µg of DNA diluted in 45 µl dMEM and then mixed with 3 µl ViaFect™ (Promega, USA) transfection reagent added and incubate for 20 minutes ...
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bioRxiv - Cell Biology 2022Quote: ... approximately 200,000 HEK-293T cells were transfected with 1 μg of plasmid DNA complexed to 3 μL of FuGENE HD (Promega, Cat: E2311). 16-20 hours post transfection the cells were dissociated from the plastic using cell dissociation buffer (Gibco Cat ...
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bioRxiv - Evolutionary Biology 2023Quote: The 419 bp sequence of 3’UTR containing predicted target sites for miR-252 in CcTRPM was cloned into the pmirGLO vector (Promega, Wisconsin, USA) downstream of the luciferase gene to construct the recombinant plasmid of CcTRPM-3’UTR-pmirGLO using the pEASY®-Basic Seamless Cloning and Assembly Kit ...
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bioRxiv - Microbiology 2023Quote: The quantification of viral RNA was carried out in one-step RT-qPCR using 3 µl of the eluted RNA with the GoTaq® Probe One-step RT-qPCR System (Promega, Poland). This procedure employed in-house primers and a probe specifically tailored for the HCoV-229E N sequence ...