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Citations for Promega :
1301 - 1350 of 3800 citations for 7H 9 6 Methenofuro 2 3 f oxacycloundecin 2 7 3H dione 3a 4 5 9 10 12a hexahydro 11 methyl 3 methylene 3aR 9S 11E 12aS 9CI since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2021Quote: The promoter fragment of luciferase reporter plasmid pGADD34-0.5k generated by Eurofins Japan (Tokyo, Japan) was subcloned into a pGL-3 vector (Promega, Madison, WI, USA) by restriction enzyme cutting site KpnI/HindIII ...
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bioRxiv - Molecular Biology 2021Quote: ... A total of 24 clones (4 holobionts x 3 replicates x 24 clones = three 96-well plates) from each amplicon were amplified directly with GoTaq polymerase (Promega, Lyon France) in a 25 μL reaction volume using the following PCR parameters ...
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bioRxiv - Cell Biology 2022Quote: ... Control and treated cells were cultured for 1 day in 96-well plates then treated with small molecules for 3 consecutive days before incubation with 120 μL fresh media containing 20 μL of CellTiter 96® AQueous One Solution (Promega, G3580) for 1 to 4 hours at 37°C in a humidified ...
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bioRxiv - Neuroscience 2023Quote: ... Cells were transfected with 1 µg of DNA diluted in 45 µl dMEM and then mixed with 3 µl ViaFect™ (Promega, USA) transfection reagent added and incubate for 20 minutes ...
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bioRxiv - Cell Biology 2022Quote: ... approximately 200,000 HEK-293T cells were transfected with 1 μg of plasmid DNA complexed to 3 μL of FuGENE HD (Promega, Cat: E2311). 16-20 hours post transfection the cells were dissociated from the plastic using cell dissociation buffer (Gibco Cat ...
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bioRxiv - Evolutionary Biology 2023Quote: The 419 bp sequence of 3’UTR containing predicted target sites for miR-252 in CcTRPM was cloned into the pmirGLO vector (Promega, Wisconsin, USA) downstream of the luciferase gene to construct the recombinant plasmid of CcTRPM-3’UTR-pmirGLO using the pEASY®-Basic Seamless Cloning and Assembly Kit ...
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bioRxiv - Microbiology 2023Quote: The quantification of viral RNA was carried out in one-step RT-qPCR using 3 µl of the eluted RNA with the GoTaq® Probe One-step RT-qPCR System (Promega, Poland). This procedure employed in-house primers and a probe specifically tailored for the HCoV-229E N sequence ...
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bioRxiv - Genomics 2024Quote: ... and 1,750 ng landing pad G384A vector template using 6 μL Fugene 6 (Promega) following the manufacturer’s protocol ...
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bioRxiv - Immunology 2024Quote: ... 6 µg of plasmid DNA was then transfected using FuGENE 6 transfection reagent (Promega) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: Plasmids encoding each vector were transfected into HEK293 cells seeded in 10 cm plates using Fugene 6™ (Promega, Madison, WI) or polyethyleneimine ...
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bioRxiv - Microbiology 2023Quote: ... Three μL (6 ng on average) of template DNA was added to 5 μL 5x GoTaq Green Reaction Mix Buffer (Promega, Madison, Wisconsin, USA), 0.625 units of GoTaq DNA Polymerase (Promega ...
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bioRxiv - Cell Biology 2021Quote: ... or Fugene 6 (Promega #E2691) for 5 hours ...
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bioRxiv - Cell Biology 2020Quote: ... FuGENE 6 Transfection Reagent (Promega) was used according to the manufacturer’s protocol and the phenotype inspected 24 or 48 h after transfection ...
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bioRxiv - Genomics 2020Quote: ... using Fugene 6 (Promega, E2691). Virus was then harvested and applied to Scc1-EGFP-AID/dCas9-KRAB cells ...
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bioRxiv - Neuroscience 2020Quote: ... using FuGENE®-6 (Promega). Supernatants containing viral particles were aseptically collected 3 days post-transfection ...
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bioRxiv - Cell Biology 2021Quote: ... using FuGENE 6 (Promega; E2691). Lentiviral particles were harvested and concentrated using a Lenti-X Concentrator (Clontech ...
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bioRxiv - Microbiology 2021Quote: ... using Fugene 6 (Promega # E2691), following the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2022Quote: ... using Fugene 6 (Promega #E2691) following manufacturer’s instructions ...
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bioRxiv - Bioengineering 2020Quote: ... Trypsin (6 ng/ml, Promega) in NH4HCO3 (50 mM ...
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bioRxiv - Microbiology 2021Quote: ... using FuGENE 6 (Promega, # E2311). 40 hours post-transfection ...
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bioRxiv - Cancer Biology 2019Quote: ... FuGENE 6 Transfection Reagent (Promega) was used for DNA transfection ...
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bioRxiv - Microbiology 2020Quote: ... or Fugene 6 (Promega, UK) and virus containing supernatants harvested 3 days later ...
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bioRxiv - Neuroscience 2021Quote: ... or Fugene® 6 (Promega) in a 3:1 ratio with cDNA mix ...
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bioRxiv - Cancer Biology 2021Quote: ... 6 μl of FuGene (Promega) transfection reagent was added ...
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bioRxiv - Microbiology 2020Quote: ... using Fugene 6 (Promega, # E2311). Variants of plasmids encoding spike included the wild-type pCMV-S ...
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bioRxiv - Cell Biology 2022Quote: ... using Fugene 6 reagent (Promega). The culture supernatant was centrifuged ...
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bioRxiv - Microbiology 2023Quote: ... using FuGene®6 (Promega) according to the manufacturer’s instructions (Fodor et al. ...
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bioRxiv - Genomics 2020Quote: ... Next nuclei were pelleted and the transposition reaction was performed incubating the lysate for 30 min at 37 °C under agitation in the presence of Transposition mixture (Tris-HCl pH 7.6 10 mM, MgCl2 5 mM, dimethyl formamide 10%, Tn5 enzyme 100 nM – Illumina #20018704, digitonin 0.01% - Promega #G9441, Tween-20 0.1%, PBS 33%). The transposition reaction was purified (Qiagen MinElute PCR Purification kit ...
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bioRxiv - Molecular Biology 2021Quote: 5–10 oocytes were lysed by vigorous shaking and pipetting in Passive Lysis Buffer (Promega, E1980), using 20 µl per oocyte ...
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bioRxiv - Biophysics 2024Quote: ... gel plugs were crushed into small pieces and 5–10 μg of sequencing-grade trypsin (Promega) per ∼100 μg protein were added ...
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bioRxiv - Systems Biology 2023Quote: ... Half of the lysis was treated with 5 µl of 10 mg/ml Rnase (Promega:527491) and both lystates (+/- RNase ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... at 10 μM for CSNK2A1 or 5 μM for CSNK2A2 in Tracer Dilution Buffer (Promega N291B) was added to all wells ...
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bioRxiv - Cancer Biology 2022Quote: ... and N-glycans were released following the addition of PNGase F (Promega) in PBS ...
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bioRxiv - Cancer Biology 2023Quote: ... the condensed supernatant was first incubated with PNGase F (Promega, Walldorf, Germany), heat-inactivated ...
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bioRxiv - Genetics 2020Quote: ... 2.4 µl of RQ1 DNase Buffer and 2 µl of RQ1 RNase-Free DNase (M6101, Promega) was added to each RNA sample and incubated for 15 minutes at 37°C ...
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bioRxiv - Evolutionary Biology 2020Quote: ... cDNA was synthesized from 2 μL of the eluted RNA using M-MLV reverse transcriptase (Promega) and random hexamer primers ...
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bioRxiv - Developmental Biology 2021Quote: ... and the resulting mRNAs (2 μg) were translated in 50 μl of rabbit reticulocyte lysate (Promega). Flag-tagged proteins were purified by immunoprecipitation with anti-Flag M2 antibody (Sigma ...
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bioRxiv - Molecular Biology 2021Quote: ... Viral RNA of SARS-COV-2 was detected by TaqMan®-based Real-Time PCR (Promega) using a CDC protocol with two sets of primer/probe which amplifies virus nucleocapsid (N ...
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bioRxiv - Molecular Biology 2021Quote: ... thiopropyl sepharose beads were incubated overnight at 37°C with 2 μg trypsin Gold (Promega, #V5280) in 50 mM NH4HCO3 ...
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bioRxiv - Genomics 2019Quote: ... 2 μl of culture was added to 50 μl of 1X Passive Lysis Buffer (Promega #E1941) which was aliquoted in a reader plate (Corning #CLS3912) ...
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bioRxiv - Cancer Biology 2019Quote: ... HEK293 or MIA PaCa-2 cells were transfected with different AGO2 constructs using Fugene HD (Promega) or Lipofectamine 3000 (Invitrogen ...
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bioRxiv - Genomics 2019Quote: ... 2 μg of each of these reporter plasmids along with 100 ng of pRL-TK (Promega), a Renilla luciferase expression plasmid to control for transfection efficiency ...
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bioRxiv - Genetics 2021Quote: ... The purified DNA was PCR amplified using 2× GoTaq Colorless Master Mix (Promega, Madison, WI, USA), and PCR product was extracted as above to eliminate primer-dimers ...
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bioRxiv - Plant Biology 2021Quote: ... 8 µL of a mix consisting of 2 µL deoxyribonucleotide triphosphate (dNTPs, Promega, Madison, Wisconsin, US), 4 µL 5x RT-buffer ...
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bioRxiv - Plant Biology 2020Quote: ... were digested for 12 hr at 37 °C with 2 mg of sequencing-grade trypsin (Promega), followed by a second incubation for 6 hr with an additional 2 mg of trypsin ...
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bioRxiv - Plant Biology 2020Quote: ... cDNA was synthesized from 2 μg of total RNA with the MMLV kit (Promega, Madison, WI). Semi-quantitative PCR was performed within the linear amplification phase using a recombinant Taq Polymerase (Invitrogen ...
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bioRxiv - Plant Biology 2020Quote: ... 2 μg RNA was used for cDNA synthesis with M-MLV Reverse Transcriptase (Promega, Madison, USA) and 1 μL of the obtained cDNA was used for real-time PCR with SYBR™ green master mix (Bioline ...
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bioRxiv - Microbiology 2020Quote: ... The lysate was then diluted to <2 M urea and incubated with 50 μg trypsin (Promega) overnight at 37 °C under agitation ...
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bioRxiv - Microbiology 2020Quote: ... Urea was diluted to 2 M with 100 mM Tris-HCl pH 7.0 and trypsin (Promega) was added at a mass ratio of 1:100 (trypsin:protein) ...
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bioRxiv - Neuroscience 2020Quote: ... all cells were incubated in the following: mouse anti-βIII tubulin (1:1000; 2 hr; Promega) and goat anti-mouse Alexa 546 (1:1000 ...