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Citations for Promega :
1251 - 1300 of 5567 citations for Recombinant Human Epithelial Cell Adhesion Molecule His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2023Quote: ... cells were washed once in PBS and lysed in 35 μL 1× Luciferase Cell culture lysis buffer (E1531, Promega) for 15 min at room temperature and centrifuged for 10 min at 4°C ...
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bioRxiv - Molecular Biology 2023Quote: ... Cell number was measured at the indicated time intervals with CellTiter-Glo® Luminescent Cell Viability Assay (Promega #G7571) following manufacturer’s instructions.
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bioRxiv - Cancer Biology 2023Quote: ... cells were harvested and luciferase activity of cell lysates was measured using the Dual-Luciferase Reporter Assay System (Promega) in a GloMax 20/20 Luminometer (Promega ...
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bioRxiv - Biochemistry 2023Quote: ... Cell proliferation was measured daily using the MTS assay (CellTiter 96 Aqueous Non-Radioactive Cell Proliferation Assay; Promega, G5430) across 3 d (days 1 – 3) ...
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bioRxiv - Cancer Biology 2024Quote: IC50 was measured by quantifying the percentage of live cells using a CellTiter-Fluor™ Cell Viability Assay (Promega) according to manufacturer’s instructions ...
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bioRxiv - Biophysics 2024Quote: ... The next day cells were treated with compounds and assessed for cell growth 5 days later using CellTiterGlo (Promega).
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An mRNA processing pathway suppresses metastasis by governing translational control from the nucleusbioRxiv - Cancer Biology 2021Quote: ... after which the media was changed and the cell viability was measured 72 hours later using CellTiter-Glo Luminescent Cell Viability Assay (Promega) in 6 biological replicates ...
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bioRxiv - Biochemistry 2020Quote: 2.5 μg of bacmid DNA per construct obtained from amplification in DH10BacY cells were used to transfect 1 Mil Sf9 cells/per construct using Fugene transfection reagent (FuGENE HD, Promega). Virus at P0 was harvested and used to produce a stock Virus P1 solution to further infect 1 lt culture of Sf9 cells at 0.8-1 Mil/ml in SF921 medium containing Pen/Strep ...
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bioRxiv - Cancer Biology 2021Quote: ... or CTX were added to target cells along with the Jurkat NFAT-luc FcγRIIIa-expressing cell line (Jur-FcγRIIIa; Promega, Madison, WI) at a ratio of 15:1 ...
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bioRxiv - Cancer Biology 2021Quote: Cell viability 72 hours after the completion of drug treatment was measured with Cell Titer Blue (G8081, Promega, Fitchburg, WI) cell viability assay to quantify the response of HCC cells to chemotherapeutic treatment under varying TME conditions (such as stiffness and hypoxia) ...
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bioRxiv - Systems Biology 2020Quote: The cytotoxicity of the the HSP90 inhibitor was assured by cell viability assay using CellTiter-Glo® Luminescent Cell Viability Assay according to manufacturer’s instruction (Promega). The activity of untreated cells was set as 100% and cells were treated with different concentrations of 17-AAG ...
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bioRxiv - Cancer Biology 2021Quote: ... 40 000 cells were seeded/well and ATP levels were measured by CellTiter-Glo Luminescent (CTG) Cell Viability Assay (Promega) following manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... concurrent cytotoxicity studies were performed using the same cells and compound exposure and cell number was determined using CellTiter-Glo (Promega). The control compounds ACV and ganciclovir (GCV ...
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bioRxiv - Developmental Biology 2020Quote: ... neural stem / progenitor cells were seeded in 96-well coated plates (1×104 cells per well) and RealTime-GloTM MT Cell Viability Assay (Promega) was used with the continuous-read protocol at 0 ...
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bioRxiv - Molecular Biology 2020Quote: ... To knock-down cells we mixed 10 μg of dsRNA per million cells with 7.5 μl of Fugene HD (Promega; E2311) in serum-free media and we incubated the dsRNA cocktail with exponentially growing cells for at least 3 days.
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bioRxiv - Microbiology 2020Quote: ... cell viability was assessed by measruring ATP levels in cells lysates with a commercially available kit (CellTiter-Glo®, Promega).
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bioRxiv - Molecular Biology 2019Quote: ... Cells were also reverse-transfected in identical manner to determine cell viability 72 h post-transfection using CellTiter-Glo (Promega) following manufacturer’s recommendations.
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bioRxiv - Genetics 2019Quote: All luciferase reporter assays were performed either 24 (SH-SY5Y cells) or 48 hours (Primary cells) after transfection as per manufacturer’s instructions (Promega, UK). Luciferase expression was quantified using Dual Luciferase Reporter assay system and a GloMax 96 microplate luminometer.
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bioRxiv - Molecular Biology 2019Quote: ... we performed titration tests for cell viability measuring ATP levels with CellTiter-Glo Luminescent Cell Viability Assay (Promega, Madison, WI). We determined a lethal concentration defined as 50% death after 24h treatment (LC50) ...
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bioRxiv - Cancer Biology 2019Quote: ... The number of viable cells was measured after 72 hrs incubation using a CellTiter Glo Luminescent Cell Viability Assay kit (Promega) with a SpectraMax plate reader (Molecular Devices ...
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bioRxiv - Bioengineering 2021Quote: ... The response of the liver organoids was assessed by quantifying relative ATP activity using the Cell-Titer Glo Luminescent Cell Viability assay (Promega) prepared according to manufacturer’s protocol ...
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bioRxiv - Bioengineering 2020Quote: ... lysozyme secretion and cell viability were assessed using Lysozyme Assay Kit (EnzChek) and CellTiter-Glo 3D (CTG 3D) Cell Viability Assay (Promega), respectively ...
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bioRxiv - Cancer Biology 2020Quote: ... The viability of cells was measured after 3 days incubation using a CellTiter-Glo Luminescent Cell Viability Assay kit (Promega) following the manufacturer’s protocol ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... the cells were washed with PBS and lysed with 50 μL of the cell culture lysis reagent (Promega, Madison, WI). For the SARS-CoV-2 titers ...
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bioRxiv - Microbiology 2020Quote: Huh7.5.1 cells were treated with different concentrations of RG7834 for 3 days and viability was measured using Cell Titer Glo (Promega) according to the manufacturer’s instructions.
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bioRxiv - Cancer Biology 2020Quote: ... cells were treated in triplicates with increasing doses of in-house small molecule inhibitor drugs and a DMSO vehicle control for 3 days and subsequently assessed for cell viability by measurement of ATP with CellTiter-Glo Luminescent Cell Viability Assay (Promega). Signal intensity was read on a Glomax™ 96 Microplate Luminometer (Promega ...
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bioRxiv - Immunology 2021Quote: ... cells13 for 48 hours after which cells were washed with PBS and lysed with Luciferase Cell Culture Lysis 5× reagent (Promega). Nanoluc Luciferase activity in lysates was measured using the Nano-Glo Luciferase Assay System (Promega ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... Recombinant baculovirus was produced by transfecting recombinant bacmids (2-3 μg) into Sf9 cells (5 mL, density of 4×105 cells per mL) using FuGENE HD Transfection Reagent (Promega) and Opti-MEM Reduced Serum Media (Thermo Fisher Scientific) ...
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bioRxiv - Molecular Biology 2021Quote: ... the culture supernatant was removed and cells were lysed by incubation for 30 min at room temperature with Cell Culture Lysis Reagent (Promega). Next ...
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bioRxiv - Molecular Biology 2020Quote: For the analysis of cell vitality of Calu-3 cells treated with camostat mesylate or FOY-251 the CellTiter-Glo Luminescent Cell Viability Assay kit (Promega) was used ...
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bioRxiv - Microbiology 2020Quote: ... was added to each well and incubated for 2 min or cells were lysed with Cell lysis buffer (Promega E1500) and placed on a rocker for 15 min ...
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bioRxiv - Microbiology 2020Quote: ... the target cells were resuspended at 3×105/mL in prewarmed culture medium that contains EnduRen live cell substrate (Promega) at a final concentration of 17 ng/ml and incubated for 30 min ...
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bioRxiv - Cancer Biology 2020Quote: HF3016 cells were plated in individual wells of a 96-well plate and viable cells were quantified using CellTiter-Glo 3D Cell Viability Assay (Promega) in triplicate wells as per manufacturer’s instructions ...
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bioRxiv - Biophysics 2020Quote: ... The cell viability at the same wells was determined by using CellTiter-Glo Luminescent Cell Viability Assay Kit (# G7571, Promega). Neutralization efficiency was calculated as relative fluorescence to the conditioned media collected from mock-transfected cells.
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bioRxiv - Cell Biology 2020Quote: ... Cells were resuspended at 2 x 106 cell mL-1 in complete media and HaloTag substrate conjugated to TMR (Promega) was added at a final concentration of 5 μM ...
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bioRxiv - Cancer Biology 2022Quote: Relative cell viability was measured by MTS assays using the CellTiter 96® AQueous One Solution Cell Proliferation Assay (Promega). Briefly ...
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bioRxiv - Cancer Biology 2022Quote: Half-maximal inhibitory concentrations (IC50) of binimetinib (Selleckchem, MEK162) were determined for each cell line using CellTiter-Glo (CTG) luminescent cell viability assay (Promega). Cells were seeded at density of 10,000 (SKMEL2 ...
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bioRxiv - Cell Biology 2022Quote: In order to confirm that a combination of two compounds did not also lead to increased cell toxicity Vero-TMPRSS2 cell viability was measured using CellTiter-Glo reagent (Promega). Vero-TMPRSS2 cells were seeded overnight at 10,000 cells/well in 96-well clear CellBind plates (Sigma Aldrich) ...
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bioRxiv - Immunology 2022Quote: Lytic cell death was determined by measuring LDH release from cell-free supernatants using a colorimetric assay (Promega or Takara) according to the manufacturer’s protocol ...
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bioRxiv - Immunology 2022Quote: ... reduction in cell viability was determined as reduction of cellular ATP levels by using the CellTiter-Glo Luminescent Cell Viability Assay (Promega). Cells were treated with 5 µM nigericin as a positive control for inflammasome activation or 0.5% DMSO as a negative control ...
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bioRxiv - Immunology 2022Quote: ... cells9 for 48 hours after which cells were washed with PBS and lysed with Luciferase Cell Culture Lysis 5× reagent (Promega). Nanoluc Luciferase activity in lysates was measured using the Nano-Glo Luciferase Assay System (Promega ...
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bioRxiv - Immunology 2022Quote: ... for 48 h after which cells were washed with PBS and lysed with Luciferase Cell Culture Lysis 5× reagent (Promega). Nanoluc Luciferase activity in lysates was measured using the Nano-Glo Luciferase Assay System (Promega ...
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bioRxiv - Molecular Biology 2022Quote: Total RNA was isolated from control (NTC) or DDX39B-depleted (Sh3 or Sh5) CD4+ T cells using ReliaPrep RNA Cell Miniprep System (Promega), and treated in-column with DNase I following the manufacturer’s recommendations ...
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Combining LSD1 and JAK-STAT inhibition targets Down syndrome-associated myeloid leukemia at its corebioRxiv - Cancer Biology 2022Quote: ... Cell viability was determined on day 6 using the CellTiter-Glo® 2.0 Cell Viability Assay (Promega, Madison, WI, USA) according to the manufacturer’s recommendations ...
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bioRxiv - Microbiology 2021Quote: ... Medium was removed from the wells and the cells were lysed with 25 μl per well of 1X cell lysis reagent (Promega), shaken at 400 rpm for 15 min at room temperature ...
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bioRxiv - Microbiology 2020Quote: Cell viability assays in response to drug or DMSO treatment were performed using CellTiter-Glo Luminescent Cell Viability kit (Promega) in cells grown in 96-well plates (Greiner ...
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bioRxiv - Cancer Biology 2020Quote: ... viability was assessed either by live-cell imaging using the IncuCyte S3 Live Cell Analysis System (Essen Bioscience) or using CellTiter-Glo (Promega) or Crystal Violet staining (Sigma) ...
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bioRxiv - Cancer Biology 2020Quote: ... viability was assessed either by live-cell imaging using the IncuCyte S3 Live Cell Analysis System (Essen Bioscience) or using CellTiter-Glo (Promega). Luminescence was measured using an Envision Plate Reader (PerkinElmer) ...
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bioRxiv - Cancer Biology 2020Quote: ... cell numbers in both upper and lower wells were measured via either conventional cell counting or using Cell Titer Glo (Promega) as described previously (Alkan et al. ...
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bioRxiv - Biochemistry 2021Quote: ... HEK293T cells and C3H10T1/2 cells were transfected in 6-well plates with various constructs of pmirGlo vector (Promega®). Transfection was performed using Turbofect transfection reagent (Thermo Fischer scientific® ...