Labshake search
Citations for Promega :
1251 - 1300 of 5138 citations for Cow Presenilin Enhancer 2 Homolog C. Elegans PSENEN ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Genomics 2024Quote: ... with one half treated with DNase (briefly, incubation at 37°C for 30 min after adding 10 units of RQ1 RNase-free DNase, Promega) and one half not treated with DNase (considered non-DNase-treated virome samples) ...
-
bioRxiv - Bioengineering 2024Quote: ... 1 µg of total RNA was reverse-transcribed at 42 °C for 90 min and 72 °C for 15 min using the moloney murine leukemia virus (M-MLV) reverse transcriptase (Promega). Each cDNA sample was diluted 8-folds and used for qRT-PCR with the specific primer pairs of each gene listed in Supplementary Table 1 ...
-
bioRxiv - Immunology 2024Quote: ... The plates were then incubated at 37°C overnight before the quantification of luciferase activity using the Luciferase Assay System (Promega) on SoftMax Pro v7.0.2 (Molecular Devices) ...
-
bioRxiv - Molecular Biology 2024Quote: ... 30 min) and diluted to 1M urea with 200 mM ammonium bicarbonate for trypsin digestion (1 µg, 37°C, 8h, Promega). After digestion ...
-
bioRxiv - Microbiology 2024Quote: ... proteins were alkylated in 5 mM iodoacetamide for 1 hour in the darkness at RT and digested overnight at 37°C with 1 µg trypsin (Trypsin Gold Mass Spectrometry Grade, Promega). Peptides were recovered by centrifugation ...
-
bioRxiv - Pharmacology and Toxicology 2024Quote: ... Plates were then incubated for 72 h at 37°C and 5% CO2 and cell viability determined by CellTiter-Glo Luminescent Cell Viability Assay (Promega). In short ...
-
bioRxiv - Cancer Biology 2024Quote: ... In-solution digestion was performed overnight on a shaker set at 37 °C using sequencing grade trypsin (Promega, Madison, WI) at ∼1:25 w/w enzyme/protein ratio ...
-
bioRxiv - Genomics 2024Quote: ... and a second antigen retrieval step at 37°C using proteinase K (20 μg/ml in TBS, pH 8, V3021, Promega), the slides were blocked as described above ...
-
bioRxiv - Biochemistry 2020Quote: ... GDP detection kit was from ProFoldin (MA, USA) and ADP-Glo™ Kinase Assay kit from Promega Corporation ...
-
bioRxiv - Genetics 2022Quote: Genomic DNA was isolated from blood samples using commercial kit (Wizard® Genomic DNA Purification Kit; Promega), following the fabricant protocol ...
-
bioRxiv - Cancer Biology 2021Quote: Trypsin digestion was carried out overnight at 37 °C with 1:50-1:100 enzyme– protein ratio of sequencing grade-modified trypsin (Promega V5111) in 50 mM NH4HCO3 pH 7.5 ...
-
bioRxiv - Cancer Biology 2021Quote: ... Proteins trapped in the suspension bed were digested in 50 mM ammonium bicarbonate with trypsin and endoproteinase Lys-C (Promega, V5073) at enzyme to protein ratio 1:25 in a 47 °C waterbath for 2 h ...
-
bioRxiv - Neuroscience 2021Quote: The protein lanes were excised from the gels and proteolytic digestion with trypsin/lys-C mix (V5073, Promega, Southampton, United Kingdom) was performed as described (Shevchenko et al. ...
-
bioRxiv - Biochemistry 2020Quote: ... Samples were diluted 10-fold with 100 mM Tris (pH 8.0) and incubated overnight at 37°C with sequencing grade trypsin (Promega, Madison, WI) added at a 1:50 enzyme:substrate ratio (wt/wt) ...
-
bioRxiv - Molecular Biology 2021Quote: ... and proteins were subjected to on-cartridge digestion with mass spec grade Trypsin/Lys-C Rapid digestion enzyme (Promega, Madison, WI) at 70°C for 1h ...
-
bioRxiv - Systems Biology 2019Quote: ... lysates were diluted in 700µl of TBS and incubated at 4°C with pre-equilibrated Magne® HaloTag® beads (Promega) or SNAP-Capture Magnetic Beads (New England Biolabs) ...
-
bioRxiv - Cell Biology 2019Quote: ... Bound fractions were then washed with 1xPBS to remove excess salt followed by RQ1 DNase treatment at 37° C for 10 minutes [4 units of RQ1 (Promega M6101) and 60 units of SUPERaseIN (ThermoFisher AM2694)] ...
-
bioRxiv - Biochemistry 2019Quote: ... Plates were then incubated at 37°C and 5% CO2 for 48 hours upon which cell viability was assessed by adding CellTiter-Glo® (Promega) to the reaction ...
-
bioRxiv - Biochemistry 2021Quote: ... Transcription of tRNApheV and tRNAleuZ was performed with 20 U T7 RNA Polymerase (37 °C for 90 min) using the Riboprobe® in vitro Transcription System (Promega) essentially as described in the instructions manual ...
-
bioRxiv - Immunology 2021Quote: ... The indicated compounds were introduced analytically using a CyBio pintool and the plates left to incubate at 37 °C prior to the addition of the CellTiter-Glo reagent (Promega, G7573) according to the manufacturer’s recommendations ...
-
bioRxiv - Immunology 2021Quote: ... 2.5 μl TDE1 and 22 μl nuclease-free water were combined and placed at 37°C for 3 min before 0.5 μl of 1% Digitonin (Promega, Cat. #G9441) was added to the master mix ...
-
bioRxiv - Microbiology 2020Quote: ... Plates were incubated at 37°C (5% CO2) for 48 h before performing CellTiter-Glo (CTG) assays as per the manufacturer’s instruction (Promega, Madison, WI). Luminescence was read on a BioTek Synergy HTX plate reader (BioTek Instruments Inc. ...
-
bioRxiv - Plant Biology 2020Quote: ... The dried gels were incubated for 16 h at 37°C in 50 mM ammonium bicarbonate containing sequence grade modified trypsin (Trypsin Gold; Promega, USA). The digested peptides were eluted from the gels with 50% acetonitrile (v/v ...
-
bioRxiv - Microbiology 2020Quote: ... The plates were cultured for two days at 37°C with 5% CO2 after which the supernatant was removed and replaced with 50 μl Nano-Glo Luciferase Substrate (Promega, Inc.). Light emission was measured in an Envision 2103 Multi-label plate reader (PerkinElmer ...
-
bioRxiv - Microbiology 2021Quote: ... Proteins were then alkylated using 15mM chloroacetamide at 37°C for 30min and further digested using sequencing-grade modified trypsin (1/25, w/w, trypsin/protein; Promega, USA) at 37°C overnight ...
-
bioRxiv - Cancer Biology 2022Quote: ... Protein was digested with Lys-C (Wako) (1:50 enzyme-to-substrate ratio) for 3 h at 25°C and with sequencing-grade modified trypsin (Promega, V5117) at 25°C for 14 h ...
-
bioRxiv - Biochemistry 2022Quote: Peptide mass spectrometry was completed after reduction with 10 mM DTT and alkylation with 55 mM iodoacetamide and protein digestion overnight at 37 °C at a 1:50 ratio of trypsin (Promega, UK). Tryptic peptides were analysed by nano-scale capillary LC-MS/MS with an Ultimate U3000 HPLC (ThermoFisher Scientific Dionex ...
-
bioRxiv - Developmental Biology 2022Quote: ... Recombinant C-terminally HA tagged Su(H) was produced using in vitro coupled transcription/translation (TNT with rabbit reticulocyte extracts; Promega Inc.) from DNA matrices generated by PCR using Su(H)-HA forward and reverse primers combined with the Su(H ...
-
bioRxiv - Cell Biology 2021Quote: ... 30 min) and diluted to 1M urea with 200mM ammonium bicarbonate for trypsin digestion (1μg, 37°C, 8 h, Promega cat # V5113).
-
bioRxiv - Cell Biology 2021Quote: ... for 4 hours at 37°C and boosted with an extra 2µl Trypsin/LysC (Promega V5073: 20ug + 80uL 50mM acetic acid) overnight at 37°C ...
-
bioRxiv - Biochemistry 2021Quote: ... RRL were incubated for 5 min at 30°C then 10 min on ice with buffer containing 1U/μl of recombinant RNasin (Promega®), 75 mM KCl 0.5 mM MgCl2 ...
-
bioRxiv - Physiology 2022Quote: ... In-gel digestion of proteins was performed overnight at 37°C using trypsin (Promega Madison, WI, USA; 40 ng per band), and the resulting peptides were extracted twice (2 x 45 min ...
-
bioRxiv - Immunology 2022Quote: ... Pseudoviruses stock were titrated by infecting 293T-ACE2 cells and luciferase activity was determined following a 44-48 h incubation period at 37°C and 5% CO2 by addition Bright-Glo Luciferase Assay System (Promega, E2650) using a microplate reader (TECAN ...
-
bioRxiv - Cell Biology 2022Quote: ... and proteins were subjected to on-cartridge digestion with mass spec grade Trypsin/Lys-C Rapid digestion enzyme (Promega, Madison, WI) at 70°C for 1 hour ...
-
Revisiting the role of Toxoplasma gondii ERK7 in the maintenance and stability of the apical complexbioRxiv - Microbiology 2021Quote: ... Overnight digestion was performed at 37 °C with 5 μl of freshly prepared trypsin (Promega; 0.2 μg/μl in TEAB 0.1M). To remove RapiGest ...
-
bioRxiv - Systems Biology 2020Quote: ... Samples were dried in a vacuum centrifuge and resuspended in 200 uL of 0.05 ug/uL Lys-C/Trypsin (Promega, WI, USA) in 25 mM Ammonium Bicarbonate ...
-
bioRxiv - Microbiology 2020Quote: ... Cells were further cultured for 72 h at 37°C with 5% CO2 and lysed in luciferase substrate buffer (Bright-Glo; Promega, Poland). Lysates were transferred onto white 96-wells plates and luciferase levels were measured on a microplate reader Gemini EM (Molecular Devices ...
-
bioRxiv - Biochemistry 2020Quote: ... Proteins were then reduced with 10 mM DTT in 100 mM Ambic for 30 min at 40 °C and alkylated with 55 mM iodoacetamide in 100 mM Ambic for 20 min at 40 °C followed by digestion with 0.3 μg trypsin (sequence grade, Promega, Madison, WI) in 50 mM Ambic for 5 h at 40 °C ...
-
bioRxiv - Microbiology 2020Quote: ... samples were heated at 80 °C for 15 min and cooled to RT before addition of 1 μg of trypsin (Promega, V511A). Samples were digested at 37 °C overnight ...
-
bioRxiv - Cancer Biology 2022Quote: ... Proteins were alkylated using 15 mM chloroacetamide at 37° C for 30 min and further digested using sequencing-grade modified trypsin (1/50 w/w, ratio trypsin/protein; Promega, USA) at 37° C for 12 hours ...
-
Reducing mitochondrial ribosomal gene expression does not alter metabolic health or lifespan in micebioRxiv - Cell Biology 2022Quote: ... The urea concentration was reduced to <2M and the protein sample was digested overnight at 37°C with 1:100 Trypsin Gold (Promega V5280). The following day ...
-
bioRxiv - Immunology 2022Quote: The Flag-TRAF6 preparations eluted from FLAG resin with 3X FLAG peptide were digested with a combination of trypsin and Lys-C protease (Promega, #V5071) using S-trap mini columns (Protifi ...
-
bioRxiv - Immunology 2022Quote: ... Proteins then were reduced with 10 mM DTT in 100 mM Ambic for 30 min at 40oC and alkylated with 55 mM iodoacetamide in 100 mM Ambic for 20 min at 4°C followed by digestion with 0.3 mg trypsin (sequence grade, Promega, Madison, WI) in 50 mM Ambic for 5 h at 4°C ...
-
bioRxiv - Neuroscience 2022Quote: ... The gel pieces were dried with 200 μl of ACN and digested with 10 ng/μl Lys-C (sequencing grade, Promega, USA) in 50 mM NH4HCO3 overnight at 37 °C (14).
-
bioRxiv - Molecular Biology 2022Quote: ... Proteins then were reduced with 10 mM DTT in 100 mM Ambic for 30 min at 40°C and alkylated with 55 mM iodoacetamide in 100 mM Ambic for 20 min at 40°C followed by digestion with 0.3 μg trypsin (sequence grade, Promega, Madison, WI) in 50 mM Ambic for 5 hours at 40°C ...
-
bioRxiv - Systems Biology 2024Quote: ... protein digestion was performed overnight by addition of 50 nL of 0.01 ng nL-1 Lys-C (MS grade, Promega, Madison, USA) and 50 nL of 0.04 ng nL-1 trypsin (Promega ...
-
bioRxiv - Biochemistry 2024Quote: ... Samples were digested at 47 °C for two hours with 600 ng of mass spectrometry grade Trypsin/LysC mix (Promega, V5113). The digested peptides were then eluted with two 35 µl increments of 0.2% formic acid in water and two more 40uL increments of 80% acetonitrile with 0.2% formic acid in water ...
-
bioRxiv - Cell Biology 2024Quote: ... images produced from HeLa cells seeded on glass-bottom Mattek dishes (P35GC-1.5-14-C) and transfected with TFG-mEGFP-FLAG at 1 µg/µL using Fugene (Promega, cat # PRE2311) for 24 hours ...
-
bioRxiv - Biochemistry 2024Quote: ... when the temperature was dropped to 22°C and isopropyl β-d-1-thiogalactopyranoside (IPTG) and L-rhamnose (Promega, Madison, WI) was added at 0.2 mM and 0.1% (w/v ...
-
bioRxiv - Biochemistry 2024Quote: ... The supernatant was diluted 5-fold with 50 mM Tris-HCl pH 8.0 and digested for 12 h at 37°C using sequencing grade trypsin (Promega cat#: V511A) at 1/100 the mass of total protein ...