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Citations for Promega :
1201 - 1250 of 2980 citations for Solute Carrier Family 12 Member 1 SLC12A1 Antibody APC since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2020Quote: Activity of the inflammatory caspases 1/4/5 was measured using a commercially available Caspase-Glo® 1 Inflammasome Assay (Promega, WI, USA) from HFFs seeded in 96-well plates (2×104 cells per well) ...
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bioRxiv - Molecular Biology 2023Quote: ... or stimulated for 10 minutes or 1 hour by BRET through activation of NanoLuc’s bioluminescence with its substrate furimazine (1:100 dilution of Promega nano-Glo Live Assay). After treatment ...
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bioRxiv - Cell Biology 2023Quote: ... a master mix was prepared by diluting the Extracellular NanoLuc Inhibitor at a 1:1000 ratio and the NanoBRET Nano-Glo Substrate at a 1:333 ratio in PBS (Promega, Madison, WI, USA). Aliquots of the Nluc substrate/inhibitor master mix (100 µl ...
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bioRxiv - Immunology 2023Quote: ... prepared by combining ONE-Glo™ EX Luciferase Assay Buffer with ONE-Glo™ EX Luciferase Assay Substrate in 1:1 ratio (Promega, USA). After measuring the signal of the Firefly luciferase in the GloMax® 20/20 Luminometer (Promega ...
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bioRxiv - Cancer Biology 2023Quote: ... 1 μg of pPAX packaging vector and 1 μg of VSV-G envelope vector using FuGENE® HD Transfection Reagent (Promega, cat.no. E2311), according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2024Quote: ... we prepared a master mix containing the membrane-permeable Nluc substrate and a membrane-impermeable Nluc inhibitor using a 1:1000 dilution of Extracellular NanoLuc Inhibitor and a 1:333 dilution of NanoBRET Nano-Glo Substrate into PBS (Promega, Madison, WI, USA). Aliquots of the Nluc substrate/inhibitor master mix (100 µl ...
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bioRxiv - Molecular Biology 2024Quote: ... and digested with Lys-C (1/200 weight of protein; Fujifilm Wako) and sequencing grade modified trypsin (1/100 weight of protein; Promega, Madison, WI, USA) at 37 °C overnight in 0.1 % Rapigest ...
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bioRxiv - Bioengineering 2024Quote: ... The samples were then diluted to a concentration of 1 M urea and underwent a second digestion step with porcine trypsin (enzyme/substrate: 1/50; Promega, Madison, WI, USA) at 37°C for 18 h ...
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bioRxiv - Microbiology 2021Quote: ... Membranes were washed with TBS-T and incubated for 60 min at room temperature with an anti-mouse or anti-rabbit horseradish peroxidase (HRP)-conjugated secondary antibody (Promega, Germany) at dilutions of 1:5000 ...
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bioRxiv - Microbiology 2023Quote: ... The membranes were blocked with PBS containing 5% skim milk and incubated with HRP-conjugated anti-human IgG1 antibodies (Promega, #W403B) overnight at 4 °C.
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bioRxiv - Molecular Biology 2023Quote: ... Western blots were run according to standard procedure and probed with streptavidin IRDye680RD (Licor 925-68079) to track biotinylated HaloTag protein and anti-HaloTag antibody (Promega G928A) to track total HaloTag protein ...
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bioRxiv - Molecular Biology 2023Quote: Western blots were run according to standard procedure and probed with streptavidin IRDye680RD (Licor 925-68079to track biotinylated HaloTag protein and anti-HaloTag antibody (Promega G928A) to track total HaloTag protein ...
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bioRxiv - Cell Biology 2024Quote: ... the gel was transferred to a PVDF membrane and western blotting was performed using an anti-Halo primary antibody (Promega, #G9211) and a goat anti-mouse secondary antibody (Jackson ImmunoResearch ...
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bioRxiv - Bioengineering 2024Quote: One hundred micrograms of each antibody were reduced with 50 mM DTT and treated with 2 µl PNGase F (Promega, USA) at 37°C for over 16h with stirring before being transferred to a new vial for LC-MS analysis ...
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bioRxiv - Biochemistry 2021Quote: ... followed by a second dilution step to ~1 M urea with 50 mM NH4HCO3 and addition of trypsin (Promega, 1:50, 37 °C, overnight). After overnight incubation ...
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bioRxiv - Immunology 2022Quote: ... and digested over-night with Lys-C-Trypsin mix (1:100 enzyme to protein ratio) and trypsin (Promega; 1:50 enzyme to protein ratio). Following the digestion step ...
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bioRxiv - Cell Biology 2020Quote: ... rabbit and mouse anti-βgal 1:500 (Cappel, Promega, Abcam), mouse anti acetylated tubulin 1:100 (Sigma) ...
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bioRxiv - Immunology 2021Quote: ... and cleaned from excess DNA with DNAse 1 enzyme (Promega). RNA samples were reverse transcribed to cDNA and an anchor sequence at the variable part of the TCR was added using single strand ligation ...
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bioRxiv - Developmental Biology 2021Quote: ... from Santa Cruz Biotechnology, mouse anti-β-Galactosidase (Z378A, 1:500) from Promega, mouse anti-MYC tag (#2276 ...
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bioRxiv - Microbiology 2020Quote: ... 1 μg total RNA and 0.5 μg random primers (Promega) were used with the GoScript™ Reverse transcriptase (Promega ...
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bioRxiv - Microbiology 2021Quote: ... 1 μg total RNA and 0.5 μg random primers (Promega) were used with the GoScript Reverse transcriptase (Promega ...
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bioRxiv - Molecular Biology 2020Quote: ... or goat anti-rabbit IgG at 1:3000 (Promega W4011). Washes were with high-salt TBST (50 mM Tris pH 7.5 400 mM NaCl 0.1% Tween-20).
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bioRxiv - Microbiology 2022Quote: ... 1 μg total RNA and 0.5 μg random primers (Promega) were used with the GoScript™ Reverse transcriptase (Promega ...
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bioRxiv - Neuroscience 2021Quote: ... 1 μL 3.5 mM Alexa Fluor 660 HaloTag ligand (Promega) was added to 200-300 μL cells and incubated at 30°C for 5 minutes ...
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bioRxiv - Genetics 2020Quote: ... and 1 ng/10ml rhFGF (Promega; Madison, WI; Cat# G5071). Cells were seeded at a density of 1.5×106 per 10 cm2 plate or 2-chamber slides (Corning Inc. ...
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bioRxiv - Microbiology 2021Quote: ... 1 μl RNasin Plus RNase inhibitor (40 U/μl, Promega) and 6.5 μl saturated trehalose] was added and incubated for 1 h ...
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bioRxiv - Systems Biology 2020Quote: ... followed by trypsin (Promega; enzyme-to-substrate ratio, 1:20)-mediated proteolysis using the same cycling scheme for 90 cycles ...
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bioRxiv - Biochemistry 2020Quote: ... and 1 μl GoScript™ reverse transcriptase (Promega cat. # A5003) was added ...
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bioRxiv - Biochemistry 2020Quote: ... T7 RNA polymerase and 1 μL RNasin RNase inhibitor (Promega) and incubated overnight at 37°C ...
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bioRxiv - Biochemistry 2020Quote: ... HRP-conjugated anti-mouse IgG (Promega, WI; 1:3000-5000), HRP-conjugated anti-rabbit IgG (Promega ...
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bioRxiv - Biochemistry 2020Quote: ... HRP-conjugated anti-rabbit IgG (Promega, WI; 1:3000-5000), HRP-conjugated anti-goat IgG (Santa Cruz ...
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bioRxiv - Cell Biology 2021Quote: ... and digested with 1 µg MS-grade trypsin (Gold, Promega) by overnight incubation at 37°C ...
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bioRxiv - Plant Biology 2021Quote: ... P40 pellets were treated with 1 μg/mL trypsin (Promega) in the presence or absence of 1% (v/v ...
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bioRxiv - Biochemistry 2021Quote: ... and 150 mM NaCl) containing protease inhibitor cocktail (1×) (Promega), followed by cell lysis by incubation on ice for 20 min ...
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bioRxiv - Cell Biology 2021Quote: ... HaloTag (1:1000 dilution in 0.5% milk, Promega, Cat#: G9211), GAPDH (1:100,000 dilution in 0.5% milk ...
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bioRxiv - Microbiology 2020Quote: ... 1 μl dNTP Mix (10 mM; Promega, Leiden, the Netherlands), 1 U of Phusion Green High-Fidelity DNA Polymerase (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2021Quote: ... or anti-mouse IgG-HRP conjugate (1:10,000; Promega W402B) by incubating membranes for 2 h at room temperature ...
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bioRxiv - Neuroscience 2020Quote: ... 1 µg of pGL4.33[luc2P/SRE/Hygro] Vector DNA (Promega), and indicated amounts of human Scrib constructs (pCDNA3-eGFP-Scrib ...
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bioRxiv - Microbiology 2021Quote: ... in a 3:1 ratio of FuGENE®HD (Promega) transfection reagent to DNA ...
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bioRxiv - Neuroscience 2020Quote: ... β III tubulin (anti-mouse, dilution 1:20,000, Promega, Madison, WI), and NR2B (anti-rabbit ...
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bioRxiv - Cell Biology 2020Quote: ... and digested with 1 µg of sequencing-grade trypsin (Promega) for 2 h at 37°C followed by the addition of another 1 µg aliquot of the enzyme and incubation at 37°C overnight ...
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bioRxiv - Cell Biology 2020Quote: ... 1 mM EDTA) containing 10 µg trypsin (sequencing grade, Promega) and rotated end-over-end overnight (12 h ...
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bioRxiv - Cell Biology 2020Quote: ... Lysates were prepared in 1 × Reporter lysis buffer (Promega, E2510), and Luciferase assays were performed according to the manufacturer’s protocol (Promega ...
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bioRxiv - Neuroscience 2022Quote: ... using GoTaq® Probe 1-Step RT-qPCR System (Promega). The CDC SARS-CoV-2 nucleoprotein N2 primer set was used for amplification ...
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bioRxiv - Biophysics 2022Quote: ... and labelled with 1 µM TMR-HaloTag ligand (Promega, G8251) and 0.12 µM SiR-SNAP-Tag ligand (NEB ...
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bioRxiv - Microbiology 2022Quote: ... first with 1 µg r-LysC Mass Spec Grade (Promega) for 4 h at 30°C and then samples were diluted below 2 M urea with 100 mM Tris HCl pH 8.5 and 1 µg Sequencing Grade Modified Trypsin was added for the second digestion overnight at 37°C ...
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bioRxiv - Cell Biology 2022Quote: ... Sample B was treated with 1 µg/ml trypsin (Promega). Sample C was pre-treated with 1% Triton X-100 (EMD-Millipore ...
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bioRxiv - Immunology 2022Quote: ... Proteins were digested with 20:1 protein:trypsin (Trypsin Gold, Promega) in 50 mM ammonium bicarbonate for 3 hours at 47 °C before adding an additional 1 µg of trypsin and digesting for a further 1 hour at 47 °C ...
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bioRxiv - Cell Biology 2022Quote: ... 1 mM dithiothreitol and 1U/µl RNasin ribonuclease inhibitor (Promega) in nuclease-free water ...
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bioRxiv - Bioengineering 2022Quote: ... and then with trypsin (V5111, Promega, WI, USA; 1:100). The samples were then dried in a vacuum centrifuge for 1.5h at RT and then resuspended in running buffer ...