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Citations for Promega :
1201 - 1250 of 2119 citations for Porcine Circovirus 2 PCV2 Protein since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2022Quote: ... Protein was then digested with 0.5 µg Sequencing Grade Modified Trypsin (V5111 - Promega, Madison, Wisconsin, USA). Following the overnight digestion at 37°C ...
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bioRxiv - Plant Biology 2023Quote: ... proteins were alkylated with iodoacetamide and digested with Trypsin/Lys-C Mix (Promega, Madison, WI, USA), according to the manufacturer’s instructions ...
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bioRxiv - Systems Biology 2024Quote: ... pH 8.0 were added and proteins were digested with 1.5 μl trypsin (0.5 μg/μl; Promega) with constant shaking at 1,100 rpm and incubation at 37 °C on a ThermoMixer C incubator for 4 h ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... LgBiT protein either purified in-house (His-LgBiT) or purchased from Promega (N401A, 200 µM stock) was diluted to 20 pM in PBS pH 7.4 solution and dispensed into white 96 well non-binding surface plate (Corning #3600 ...
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bioRxiv - Immunology 2024Quote: ... PKA activation in H9c2 cells was measured using SignaTECT cAMP-dependent protein kinase assay system (Promega) according to the manufacturer’s instruction ...
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bioRxiv - Biophysics 2024Quote: ... The purified fused protein was cleaved by treating it with Factor Xa (FXa; Promega, Madison, WI) at room temperature ...
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bioRxiv - Microbiology 2024Quote: ... Cellular proteins were then degraded with 20 µl of 20 mg/mL Proteinase K Solution (Promega) and incubation at 56°C for 15 minutes ...
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bioRxiv - Microbiology 2024Quote: ... Proteins were alkylated in-gel prior to digestion with 100 ng trypsin (modified sequencing grade, Promega) overnight at 37°C ...
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bioRxiv - Immunology 2024Quote: ... Proteins were digested overnight at 37°C with either trypsin (1:50 μg/μg protease:protein) (Promega) or Chymotrypsin (1:70 µg/µg protease:protein ...
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bioRxiv - Neuroscience 2024Quote: ... DMRTA2 WT and DMRTA2R116P proteins were also produced using the Transcription and Translation (TnT) system (Promega). EMSAs were performed as described previously (Van Lint et al. ...
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bioRxiv - Biochemistry 2024Quote: ... Proteins were digested with Lys-C (Wako Chemicals) and trypsin (1:40 ratio, Trypsin Gold, Promega). After complete digestion had been confirmed by nanoLC-MS/MS (<7% missed cleavage sites) ...
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bioRxiv - Molecular Biology 2024Quote: ... and proteins were subjected to overnight digestion with mass spec grade Trypsin/Lys-C mix (Promega). Following digestion ...
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bioRxiv - Plant Biology 2024Quote: ... purified 6xHis-SYMRK-KD proteins were in vitro phosphorylated and subjected to in-sol trypsin (Promega) digestion ...
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bioRxiv - Neuroscience 2024Quote: ... available in the kit of the TnT T7 Insect Cell Extract Protein Expression System (L1101, Promega), according to manufacturer instructions ...
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bioRxiv - Biochemistry 2024Quote: ... Cells expressing Halo proteins were stained with HaloTag® TMRDirect™ Ligand (Promega, Madison, WI, USA) overnight in their regular culture medium ...
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bioRxiv - Genomics 2024Quote: ... eGFP-IVT employs the TNT SP6 High-Yield Wheat Germ Protein Expression System (Promega, Cat# L3260) to express SP6-driven ...
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bioRxiv - Cancer Biology 2021Quote: ... 2 µg of RNA was subjected to reverse transcription using M-MLV enzyme (Promega #M1705), dNTP mix 100 mM each (BLIRT #RP65 ...
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bioRxiv - Bioengineering 2021Quote: ... and 1 and 2 μL of 50 ng/μL trypsin (in 100 mM TEABC, Promega) was added to the wells with single cells and two hundred carrier cells ...
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bioRxiv - Cell Biology 2021Quote: ... treated with 3 units/sample of DNase I for 2 hours (Promega, Madison, WI, USA), and 1-3μl aliquots were taken for reverse transcription (RT ...
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bioRxiv - Microbiology 2020Quote: ... Amplification was performed in 50 μl final volume using 2 U of GoTaq Polymerase (Promega) and 100 ng of DNA template in each reaction ...
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bioRxiv - Microbiology 2020Quote: ... Amplification was performed in 50 μl final volume using 2 U of GoTaq Polymerase (Promega) and 100 ng of Nichols DNA template ...
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bioRxiv - Cell Biology 2021Quote: ... and cloned into the XhoI and HindIII sites in the psiCHECK™-2 Vector (Promega) resulting in the plasmid pTal1-Luc ...
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bioRxiv - Molecular Biology 2022Quote: ... 2 μg of total RNA was used for cDNA synthesis with AMV reverse Transcriptase (Promega) together with 20 pmol of oligonucleotides O135 (5’-TAGCGGCTGATGTTGAACTG-3’ ...
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bioRxiv - Immunology 2022Quote: ... transfected with ISD (21) at 2 µ g per dish using ViaFect (Promega, Madison, WI) at 3 µ L per 1 µ g of ISD based on manufacturer protocol ...
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bioRxiv - Immunology 2022Quote: ... transfection with ISD (21) at 2 µ g per dish using ViaFect (Promega, Madison, WI) at 3 µ L per 1 µ g of ISD based on manufacturer recommendations ...
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bioRxiv - Developmental Biology 2022Quote: ... The samples were subjected to digestion with LysC (Wako) for 2 h and trypsin (Promega) overnight at 37°C at a 1:50 enzyme ...
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bioRxiv - Molecular Biology 2020Quote: ... 2 μl of the resulting mRNA was mixed with 1 μl of FluoroTect GreenLys (Promega) and 3 μl of the wheat germ extract and then transferred under 50 μl of the translation buffer (1x SUB-AMIX SGC ...
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bioRxiv - Microbiology 2021Quote: ... The PCR products were cloned downstream of Renilla luciferase of psiCHECK(TM)-2 vector (Promega) using XhoI and NotI sites.
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bioRxiv - Microbiology 2020Quote: ... cells were transiently transfected with 2 μg DNA using FuGENE® HD (Promega, Madison, WI). Whole-cell patch-clamp recordings were performed at room temperature ...
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bioRxiv - Molecular Biology 2020Quote: Seedlings were sprayed with 2 mM Luciferin (VivoGlo™ Luciferin, In Vivo Grade, Promega, Netherlands) one day before they were released either into continuous light or dark ...
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bioRxiv - Molecular Biology 2023Quote: ... Each DNA family was amplified with 2×GoTaq® Hot Start Green Master Mix (Promega) using 0.8–2.2 ng of germline and somatic DNA and the primer pair according to the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2023Quote: ... A volume of 2 µL of the samples was diluted in Glo lysis buffer (Promega) and incubated in a 1:1 ratio for 3 min in the dark with NanoGlo substrate ...
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bioRxiv - Systems Biology 2023Quote: ... 2 mg of total RNA was used for cDNA synthesis with MMLV reverse transcriptase (Promega) in final 50 mL volume ...
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bioRxiv - Cancer Biology 2024Quote: ... Tryptic digest was performed in 50 mM ammonium bicarbonate buffer with 2 μg trypsin (Promega) at 37 °C overnight ...
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bioRxiv - Biochemistry 2024Quote: ... Cells were transfected with 2 µg of plasmids with FuGene HD transfection reagent (Promega #E2312) overnight ...
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bioRxiv - Cell Biology 2024Quote: ... Cells were transfected with 2 μg of bacmid DNA using Fugene HD transfection reagent (Promega) according to the manufacturer’s protocol ...
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bioRxiv - Systems Biology 2023Quote: ... and 500 µL per sample were digested using 2 µg Trypsin/LysC (Promega, Cat#V5072). After 17 h of incubation at 37°C ...
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bioRxiv - Systems Biology 2023Quote: ... 2 mg of total RNA was used for cDNA synthesis with MMLV reverse transcriptase (Promega) in final 50 mL volume ...
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bioRxiv - Biochemistry 2023Quote: ... Samples were again diluted to 2 M urea and digested with 1 µg trypsin (Promega) (1/100 ...
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bioRxiv - Systems Biology 2023Quote: The transfections were performed with a 1 mg DNA: 2 ml Fugene HD (Promega E2311) ratio ...
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bioRxiv - Physiology 2022Quote: ... 2-Deoxyglucose-6-phosphate (2DGP) was measured using a Glucose Uptake-Glo Assay Kit (Promega) following the manufacturer’s instructions.
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bioRxiv - Physiology 2022Quote: ... 2-DG uptake was measured using a Glucose Uptake-Glo Assay kit (Promega, Madison, WI).
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bioRxiv - Biochemistry 2022Quote: ... 2) chymotrypsin and lysyl endopeptidase (lys-C) (Fujifilm Wako) 3) lys-C and trypsin (Promega) and 4 ...
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bioRxiv - Microbiology 2022Quote: ... Once cooled the beads were digested overnight with 2 µg of Trypsin (Promega Catalog # V5111) at 37 °C and constant rocking.
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bioRxiv - Physiology 2022Quote: ... 2-Deoxyglucose-6-phosphate (2DGP) was measured using a Glucose Uptake-Glo Assay Kit (Promega) following the manufacturer’s instructions.
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bioRxiv - Cell Biology 2023Quote: cDNA was synthesized from 2 ug of total RNA using GoScript Reverse Transcriptase (Promega, #A5003) with random hexamers according to the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2023Quote: ... The mice received intraperitoneal (IP) injection of 2 milligrams of luciferin (Promega Corporation, Madison, WI) and subjected to luminescence imaging using an IVIS® Spectrum In Vivo Imaging System (PerkinElmer Inc ...
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bioRxiv - Biochemistry 2024Quote: ... pH 8.0) containing 2 nM 11S and 1/250 Nano-Glo Luciferase Assay Reagent (Promega) was added to the cells and background luminescence read for 5 min ...
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bioRxiv - Biophysics 2024Quote: ... cells were transiently transfected with 2 µg DNA by lipofection (FuGENEⓇ HD, Promega, Madison, WI).
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bioRxiv - Neuroscience 2024Quote: ... The brain surface was covered in 2-3% low melting point agarose (Promega, Madison, WI) in sterile saline and then capped with silicone elastomer ...