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Citations for Promega :
1201 - 1250 of 1855 citations for Nipah Virus Glycoprotein F Recombinant Protein Mouse Fc since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2021Quote: ... the proteins were digested with 0.2 µg of sequencing-grade trypsin (Promega, Madison, WI, USA) overnight at 37°C.
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bioRxiv - Developmental Biology 2022Quote: ... The 50μg of proteins were then trypsin digested overnight at 37 °C (1:25; Promega) using the filter aided sample preparation (FASP ...
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bioRxiv - Biochemistry 2022Quote: ... The proteins were digested by 0.2 μg modified grade porcine trypsin (Promega, Co., Madison, WI) during overnight incubation at 37 °C ...
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bioRxiv - Cell Biology 2022Quote: ... Protein synthesis was performed in the presence of [35S]methionine in rabbit reticulocyte lysate (Promega). Coupled translation/translocation reactions of 20 μl contained 16.5 μl of reticulocyte lysate ...
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bioRxiv - Microbiology 2022Quote: ... Proteins were alkylated in-gel prior to digestion with 100ng trypsin (modified sequencing grade, Promega) overnight at 37°C ...
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bioRxiv - Molecular Biology 2022Quote: One-hundred μl protein expression reaction of TNT SP6 Coupled Reticulocyte Lysate System (Promega, L4600) containing 1000 ng pIXHALO-bZIP plasmids and 1500 ng pIXSBP-bZIP plasmids were incubated for 3 h at 30°C ...
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bioRxiv - Plant Biology 2022Quote: In vitro translation was carried out using a wheat germ protein expression system (Promega, USA) following the manufacturer’s instruction ...
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bioRxiv - Plant Biology 2022Quote: ... Eluc activity and protein concentration in supernatant were measured using the Luciferase Assay System (Promega) and the Pierce 660 nm Protein Assay Reagent (Thermo Fisher Scientific) ...
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bioRxiv - Neuroscience 2024Quote: ... Proteins captured on magnetic beads were digested with 1.5 mg/mL trypsin (Promega Cat# V5111) in 50 mM ammonium bicarbonate overnight at 37 °C ...
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bioRxiv - Plant Biology 2024Quote: ... We further used the TNT SP6 High-Yield Wheat Germ Protein Expression System (L3260, Promega) to express the four TFs-HaloTag fusion protein ...
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bioRxiv - Microbiology 2023Quote: ... for determination of viral RNA synthesis/protein expression or 50 µl CellTiter-Glo reagent (Promega) for determination of cell viability/ATP concentration ...
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bioRxiv - Plant Biology 2024Quote: ... Protein samples eluted with biotin were digested in-solution with 1 ug Trypsin/LysC (Promega) overnight at 37°C ...
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PI3K/HSCB axis facilitates FOG1 nuclear translocation to promote erythropoiesis and megakaryopoiesisbioRxiv - Molecular Biology 2024Quote: ... Nuclear extracts of equal protein amounts were first subjected to proteolysis with trypsin (V5113, Promega) at 37°C for 16 hours ...
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bioRxiv - Cell Biology 2024Quote: ... Protein detection was done using primary antibody anti-HaloTag monoclonal antibody (1:1,000 dilution, Promega), primary antibody anti-MamE polyclonal antibody (1:3,000 dilution ...
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bioRxiv - Biochemistry 2023Quote: ... Proteins were digested for 18 to 24 hours on bead with sequencing grade trypsin (Promega) in 50 mM HEPES buffer at 1:50 trypsin to protein ratio ...
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bioRxiv - Microbiology 2023Quote: Reduced and alkylated proteins were in-gel digested with 100ng trypsin (modified sequencing grade, Promega) overnight at 37° C ...
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bioRxiv - Plant Biology 2023Quote: ... The proteins were resuspended in 100 mM NH4HCO3 and were digested with trypsin (Promega, UK) at an enzyme-to-substrate ratio of 1:25 ...
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bioRxiv - Plant Biology 2023Quote: ... Proteins were produced in vitro via the TNT SP6 Quick Coupled Transcription/Translation System (Promega) following manufacturer’s instructions ...
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bioRxiv - Biochemistry 2022Quote: ... Proteins were extracted by methanol-chloroform precipitation and digested with 1 μg of trypsin (Promega) in 100 mM EPPS (pH 8.0 ...
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bioRxiv - Genomics 2023Quote: Proteins were expressed in rabbit reticulocyte lysate (TNT T7 Quick Coupled Transcription/-Translation System, Promega) according to the manufacturer’s instructions ...
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bioRxiv - Bioengineering 2023Quote: ... 2.25 ng/uL Single-Stranded DNA Binding Protein (Sigma, cat# S3917 OR Promega, cat# M301A). 12.5 uL of master mix was mixed with 12.5 uL of 2x input oligonucleotide ...
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bioRxiv - Microbiology 2023Quote: ... followed by overnight digestion with trypsin (enzyme to protein ratio of 1:100) (Promega, Germany) at 37 °C ...
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bioRxiv - Molecular Biology 2023Quote: Proteins for CoIP were produced with the TnT Quick Coupled Transcription/Translation System (Promega, www.promega.com) according to the manufacturer’s recommendations ...
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bioRxiv - Neuroscience 2023Quote: ... Proteins were precipitated with trichloroacetic acid and digested with Lys-C (Wako) and trypsin (Promega). The peptides were then analysed using an Ultimate 3000 nano-RSLC (Thermo Scientific ...
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bioRxiv - Synthetic Biology 2023Quote: ... Bound protein was quantified using the Nano-Glo® HiBiT Extracellular Detection System (N2420, Promega). To quantify immobilized DNA in wells without protein ...
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bioRxiv - Cell Biology 2023Quote: ... Protein pellets were resuspended in the digestion buffer as previously described and trypsin (Promega, UK) digested to peptides74 ...
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bioRxiv - Genetics 2022Quote: Proteins were produced in vitro via the TNT SP6 Quick Coupled Transcription/Translation System (Promega). If two proteins were to be tested together in a single binding reaction ...
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bioRxiv - Cell Biology 2023Quote: ... and finally radiolabelled precursor proteins were synthesized with the Flexi rabbit reticulocyte lysate system (Promega). 35S-radiolabeled precursor proteins were incubated with isolated mitochondria in import buffer (3% (w/v ...
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bioRxiv - Biochemistry 2023Quote: Proteins labeled with [35S]-methionine were produced by coupled transcription and translation reactions (T7, Promega). Translation products were partially purified by ammonium sulfate precipitation [87] ...
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bioRxiv - Biochemistry 2023Quote: ... coli and HeLa cells as well as of a commercially available yeast protein digest (Promega), The ratios of the peptide amount of each species within the three mixtures are given in Table S1.
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bioRxiv - Neuroscience 2023Quote: ... Proteins were digested overnight at 37° C with 1 μg mass spectrometry grade Trypsin (Promega). The resulting peptide sample was acidified with 10% formic acid and 10% trifluoroacetic acid (TFA ...
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bioRxiv - Plant Biology 2023Quote: Proteins were expressed using the TnT Coupled Wheat Germ Extract System with SP6 promoter (Promega). Protein expression was confirmed by Western Blot ...
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bioRxiv - Cell Biology 2023Quote: ... The proteins were resuspended in 100 mM NH4HCO3 and were digested with trypsin (Promega, UK) at an enzyme-to-substrate ratio of 1:25 ...
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Arabidopsis actin-binding protein WLIM2A links PAMP-triggered immunity and cytoskeletal organizationbioRxiv - Plant Biology 2023Quote: ... Proteins were then digested overnight at 37°C with trypsin (Porcine trypsin, Promega, Fitchburg, WI). After stopping the digestion with 1% formic acid ...
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bioRxiv - Cell Biology 2023Quote: ... Test proteins were generated in vitro using the TNR quick-coupled transcription-translation kit (Promega). For radiolabeling ...
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bioRxiv - Neuroscience 2023Quote: ... proteins on beads were digested with the addition of 2 µg trypsin (Promega, Cat# V511A) for 18 h at 37°C.
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bioRxiv - Molecular Biology 2023Quote: ... we labeled HALO-tagged49 proteins with HALO-Alexa488 or HALO-TMR dyes (Promega, G1001, G8251) by incubating the concentrated protein with dye for 1 hour on ice ...
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bioRxiv - Cell Biology 2024Quote: ... Proteins on the beads were digested by adding 200 ng trypsin/Lys-C mix (Promega) at 37 °C overnight ...
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bioRxiv - Biochemistry 2024Quote: ... For protein synthesis in reticulocyte lysate the TNT® Quick Coupled Transcription/Translation System (Promega) was used and performed according to the manufacture’s protocol employing 1 mM unlabeled methionine ...
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bioRxiv - Cell Biology 2024Quote: ... cells transfected with HALO-fusion protein were supplemented with 100 nM TMR HALO ligand (Promega). Cells were fixed at RT for 20 min in 3.7% formaldehyde (FA ...
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bioRxiv - Molecular Biology 2024Quote: ... Proteins were digested by adding 5 μL of sequencing grade trypsin (0.1 mg/mL) (Promega) for three h at 37 °C ...
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bioRxiv - Microbiology 2024Quote: ... Proteins were then digested using 250 ng of trypsin enzyme (Sequencing Grade Modified Trypsin, Promega) and incubation for 1h at 37°C ...
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bioRxiv - Neuroscience 2021Quote: RNA was isolated from whole mouse brain tissue using ReliaPrep RNA Tissue Miniprep System (Promega) following the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2022Quote: Total RNA was extracted from mouse hearts using ReliaPrep™ RNA Tissue miniprep system (Promega). cDNA was synthesized using M-MLV Reverse Transcriptase (Promega) ...
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bioRxiv - Microbiology 2020Quote: ... the blots were incubated for 1h with secondary antibodies (mouse antirabbit IgG-HRP, Promega W4018) that was diluted 1:10000 in blocking buffer ...
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bioRxiv - Cancer Biology 2019Quote: ... Immunoreactive bands were detected with horseradish peroxidase-coupled anti-mouse or anti-rabbit antibodies (Promega) using the ECL system (Merck Millipore WBKLS0500) ...
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bioRxiv - Microbiology 2021Quote: ... After incubation with Goat anti-Rabbit and Goat anti-Mouse HRP-conjugated secondary antibodies (Promega), proteins were visualized using chemiluminescent peroxidase substrat detection reagents (Sigma) ...
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bioRxiv - Cell Biology 2022Quote: Primary antibodies used in this study are as listed: mouse monoclonal anti-Halo (Promega, G9211), rabbit polyclonal anti-SNAP-tag (New England BioLabs ...
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bioRxiv - Cell Biology 2021Quote: ... followed by a 1:20,000 dilution of anti-mouse IgG HRP conjugated secondary antibody (Promega) for 1 h at room temperature ...
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bioRxiv - Neuroscience 2021Quote: ... Neurite outgrowth was visualized by immunostaining with mouse anti-β III tubulin (1:1000; Promega) followed by incubation with Alexa 594-conjugated anti-mouse secondary antibodies (Invitrogen).