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Citations for Promega :
1201 - 1250 of 1647 citations for GA binding protein alpha chain GABPA Antibody since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2023Quote: Electrophoretic Mobility Shift Assays were performed using proteins expressed in the TNT T7 Coupled Reticulocyte Lysate System (Promega, WI), according to the manufactureŕs recommendation ...
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bioRxiv - Cancer Biology 2023Quote: ... Unlabeled proteins were cell-free-synthesized using the TNT T7 Quick Reaction system according to the manufacturer instructions (Promega) and incubated with purified GST fusion proteins coupled with glutathione sepharose 4B fast flow beads (GE healthcare ...
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bioRxiv - Cancer Biology 2024Quote: ... The identity of each cell line was confirmed by DNA fingerprinting via short tandem repeats at the time of mRNA and total protein lysate preparation using the PowerPlex 1.2 kit (Promega).
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bioRxiv - Cell Biology 2023Quote: ... urea was diluted to 1 M and proteins digested overnight with modified sequencing grade trypsin (Promega, Madison, WI, USA) at a 1:50 enzyme:protein ratio ...
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bioRxiv - Plant Biology 2024Quote: ... and the recombinant protein was produced using a TNT SP6 Coupled Wheat Germ Extract System (Promega, Madison, WI, USA). Magne Halo Tag Beads (Promega ...
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bioRxiv - Physiology 2022Quote: ... The proteins were cleaved into tryptic peptides by addition of 25 μl of a 0.2 μg/μl trypsin (Promega) in 50 mM ammonium bicarbonate and incubation (18 h ...
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bioRxiv - Plant Biology 2022Quote: ... ZmEREB57 fused to HaloTag was expressed using the TnT SP6 High-Yield Wheat Germ Protein Expression System (L3260) (Promega), and then purified using Magne HaloTag Beads (G7281 ...
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bioRxiv - Cell Biology 2023Quote: ... Samples were further diluted with 50 mM Tris pH 7.9 pH 8.0 to a final urea concentration of 2 M and proteins were digested with 5 µg trypsin (Promega) (1/100 ...
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bioRxiv - Molecular Biology 2022Quote: ... 50 ng/well of chaperone RTP1S,105 50 ng/well of the G protein subunit Gαolf,106–107 olfactory G protein subunit Gγ13,108 and 50 ng/well of pGloSensorTM-22F (Promega, Madison, USA).109 The utilized pGloSensor™-22F is a genetically engineered luciferase with a cAMP-binding pocket ...
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bioRxiv - Genetics 2023Quote: ... proteins were extracted by chloroform-methanol precipitation and digested with 2 μg trypsin/Lys-C mixture (Promega, Madison, WI). Samples were labeled with 10-plex tandem mass tag reagents (ThermoFisher Scientific ...
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bioRxiv - Plant Biology 2024Quote: ... proteins that underwent in vitro reactions were digested in 50 mM ammonium bicarbonate (NH4HCO3) with 0.2 µg trypsin (Promega) at 37°C overnight ...
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bioRxiv - Plant Biology 2024Quote: ... and αKNL2-CΔDNAb (1,2) fused with a FLAG tag were used for protein expression with TNT SP6 High Yield Wheat Germ (Promega) following the manufacture’s protocol ...
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bioRxiv - Molecular Biology 2024Quote: ... proteins were acetone precipitated and digested using LysC (Wako sequencing grade #125-05061) and trypsin (Promega sequencing grade #V5111), as described by Buczak et al ...
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bioRxiv - Molecular Biology 2024Quote: ... and the pulldown of HaloTag proteins was conducted according to HaloTag Mammalian Pull-Down System protocol (Promega, cat # G6504). Beads were washed thrice with 1× NEB CutSmart Buffer followed by dephosphorylation by using Quick CIP (New England Biolabs ...
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bioRxiv - Biochemistry 2024Quote: ... Protein digestion was done overnight at room temperature with trypsin (Promega V5113, 1/100 volume of 1mg/mL trypsin). 1% final concentration of TFA was added to quench the digestion ...
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bioRxiv - Immunology 2024Quote: ... Samples were digested overnight with LysC (Wako, 1:100 enzyme: protein ratio) and trypsin (Promega, 1:50 enzyme:protein ratio). Samples were desalted with a 96-well mini 20MG PROTO 300 C18 plate (HNS S18V ...
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bioRxiv - Immunology 2024Quote: ... and 1μg of plasmid expressing the respective spike protein using a 3:1 ratio of Fugene HD transfection agent (Promega).28,49 After 48 hours of incubation at 10% CO2 ...
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bioRxiv - Microbiology 2024Quote: ... The sample was processed according to the SP3 protocol36 and digested overnight with trypsin (Promega, enzyme/protein 1:50). Peptides were desalted using SBD-RPS tips as previously described37 ...
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bioRxiv - Molecular Biology 2024Quote: ... pH 8.0 to an urea concentration of 4 M and proteins were digested with 40 µg endopeptidase LysC (Promega) (1/250 ...
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bioRxiv - Molecular Biology 2024Quote: ... Samples were further diluted with 20 mM HEPES pH 8.0 to a urea concentration of 2 M and proteins were digested with 50 µg trypsin (Promega) (1/200 ...
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bioRxiv - Molecular Biology 2024Quote: ... Trypsin digestion of the protein pellet was performed using 1μg of modified sequencing grade trypsin (Cat #: V5111; Promega, USA) in 100 μL of 20 mM ammonium bicarbonate at 37 °C overnight (12 – 18 hours) ...
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bioRxiv - Neuroscience 2024Quote: ... followed by a 4-fold dilution with 50 mM HEPES buffer pH 8.5 and overnight trypsin digestion (50:1 protein: enzyme by weight, Promega) at room temperature ...
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A detailed kinetic model of Eastern equine encephalitis virus replication in a susceptible host cellbioRxiv - Systems Biology 2024Quote: NanoLuciferase protein expression was measured from triplicate collections of EEEV FL93 nLuc infected cell lysates by the manufacturer’s protocol (Promega) as previously described in [152] on a Biotek Synergy LX plate reader.
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bioRxiv - Bioengineering 2024Quote: ... tendons were first digested and then levels of glutathione protein content were measured using a GSH-Glo assay (Promega) and glutamate dehydrogenase content was measured using a glutamate dehydrogenase activity assay kit (Abcam ...
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bioRxiv - Biochemistry 2024Quote: ... laevis UVSSA variants could be produced in the TnT® SP6 High-Yield Wheat Germ Protein Expression System (Promega) according to manufacturer’s instructions and added directly to the “Extract Mixture” ...
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bioRxiv - Microbiology 2020Quote: ... As secondary antibody an anti-mouse IgG HRP conjugate (Promega, Madison, WI, USA) was used ...
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bioRxiv - Immunology 2020Quote: ... 1:3,000 dilution of HRP-conjugated anti-mouse IgG secondary antibody (W402B, Promega) was added and incubated for one additional hour ...
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bioRxiv - Microbiology 2021Quote: ... Immunodetection was carried out using rabbit polyclonal anti-Halo antibody (Promega; 1:1000), mouse anti-HA antibody (Roche ...
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bioRxiv - Microbiology 2022Quote: ... followed by a horseradish peroxidase (HRP)-conjugated anti-rabbit IgG secondary antibody (Promega). Chemiluminescence signals were detected using the GE ImageQuant LAS4000 camera (GE Healthcare Life Sciences).
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bioRxiv - Developmental Biology 2020Quote: ... The primary antibodies that were used are mouse β-Gal (1:250; Promega), mouse anti-Cora C615.16 (1:400 ...
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bioRxiv - Developmental Biology 2021Quote: The antibodies used were: Rabbit anti-pJNK pTPpY (1:500, Promega, Cat#V93B), Rat anti-Ci(1:1,000 ...
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bioRxiv - Cell Biology 2021Quote: ... and then anti-mouse IgG (H&L) alkaline phosphatase-conjugated secondary antibodies (Promega) were incubated with the blots at a dilution of 1:5,000 for 1 h at room temperature (RT) ...
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bioRxiv - Neuroscience 2023Quote: ... The following antibodies were used for immuohistochemistry: mouse anti-β-gal (Z3781, Promega), chicken anti-NeuN (266006 ...
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bioRxiv - Systems Biology 2023Quote: ... Horseradish peroxidase-conjugated secondary antibodies were purchased from Promega (anti-rabbit IgG, W4011). Membranes were stripped with 0.2 M NaOH as needed ...
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bioRxiv - Developmental Biology 2023Quote: ... and rabbit anti-Caspase-3 polyclonal antibody (1:250, Promega/Fisher, PR-G7481) overnight at 4 °C ...
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bioRxiv - Microbiology 2022Quote: ... Secondary anti-mouse IgG HRP antibody was used at 1:7000 dilution (Promega). ChemiDoc (Bio-Rad ...
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bioRxiv - Cell Biology 2022Quote: ... from Invitrogen and HRP-conjugated anti-mouse (W4021) and anti-rabbit (W4011) antibodies from Promega.
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bioRxiv - Cell Biology 2023Quote: ... Primary antibody against HaloTag® (G9211; 1:1000) was purchased from Promega (UK).
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bioRxiv - Genomics 2022Quote: ... followed by incubation with horsradish peroxidase (HRP)-conjugated secondary antibodies (Promega, Madison, WI). The chemiluminescence signals were detected using a Western blot developing machine (Fusion Solo S chemiluminescence imager ...
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bioRxiv - Immunology 2022Quote: ... Anti-Rabbit (W4018) and Anti-Mouse (W4028) secondaries antibodies were bought from Promega.
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bioRxiv - Plant Biology 2023Quote: ... incubated with the secondary antibody rabbit anti chicken IgY HRP [(Promega Corporation, G1351) 1:10000] for 2 h and again washed three times ...
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bioRxiv - Molecular Biology 2024Quote: ... the membrane was probed with a horseradish peroxidase-conjugated mouse secondary antibody (Promega). The image was visualized with SuperSignal™ West Pico PLUS Chemiluminescent Substrate (Thermo fisher Scientific ...
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bioRxiv - Developmental Biology 2024Quote: ... We used the following primary antibodies: mouse anti-LacZ (1:500 dilution, Promega) rabbit polyclonal anti- cleaved Caspase 3 (1:200 dilution ...
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bioRxiv - Neuroscience 2024Quote: ... The following antibodies were used: mouse anti–βIII-tubulin (1:2,000; Promega, G7121) and rabbit anti-synaptotagmin-2 (1:500 ...
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bioRxiv - Synthetic Biology 2024Quote: ... The anti-HaloTag antibody, (G921A, 1:500 for WB) was purchased from Promega. The Alexa Fluor™ 488 Goat anti-Mouse IgG (A11029 ...
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bioRxiv - Cell Biology 2024Quote: ... secondary antibodies for 2 hours prior to imaging by enhanced chemiluminescence (Promega ECL) and autoradiography films (GE Healthcare ...
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bioRxiv - Cell Biology 2024Quote: ... Anti-rabbit IgG HRP conjugate antibody (W401B) was purchased from Promega (Madison, WI). pGateway 3XFlag Prickle1 vector (24644 ...
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bioRxiv - Plant Biology 2020Quote: HALO-FDL1 and HALO-GST (negative control) DAP-seq samples were performed using protein expressed in the TNT rabbit reticulocyte expression system (Promega) as previously described (Bartlett et al. ...
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bioRxiv - Biophysics 2020Quote: Plasmid DNAs containing Tol2 vectors comprising GV genes and fluorescent protein genes described above and transposase mRNA were introduced to KPL-4 cells by lipofection using FuGene HD (Promega) in OptiMEM (Gibco) ...
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bioRxiv - Developmental Biology 2020Quote: In vitro translation (IVT) with 500 ng of mRNA was performed using the TnT SP6 High-Yield Protein Expression System (Promega) to synthesize tagged proteins ...