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Citations for Promega :
1201 - 1250 of 4976 citations for 6 methyl 4 oxo N phenyl 2 3 dihydro 1 4 oxathiine 5 carboxamide since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2021Quote: ... 5 units GoTaq DNA polymerase (Promega) and water to 50μl ...
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bioRxiv - Neuroscience 2022Quote: ... 5 μL ADP-Glo reagent (Promega) was added to each reaction mixture and incubated for 40 min at 25°C ...
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bioRxiv - Biochemistry 2020Quote: ... and 5 µL RNasin Plus (Promega) per sample to 800 µL lysate and 1.5-2 hours of rotation at 4°C ...
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bioRxiv - Biochemistry 2021Quote: ... 5 μM HaloTag-TMR ligand (Promega), Ready-Lyse Lysozyme (47 U/μl ...
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bioRxiv - Biophysics 2023Quote: ... 5 μL of CTG reagent (Promega G7571 ...
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bioRxiv - Immunology 2023Quote: ... 5 ng of pRL-TK (Promega), and 5 ng of pEF-Slc46 expression plasmid using GeneJuice (Millipore ...
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bioRxiv - Neuroscience 2023Quote: ... 5 mg/mL proteinase K (Promega)) and incubated overnight at 50°C ...
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bioRxiv - Cancer Biology 2024Quote: ... 5 μl of CellTiter-Blue (Promega) was added and cells were incubated for 90 minutes at 37°C before measuring fluorescence using an EnVision plate reader (PerkinElmer) ...
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bioRxiv - Cancer Biology 2021Quote: ... 1 mg/mL 50 μL aliquots of cleared lysates were incubated with 2 μL of indicated trypsin concentration (Promega) at 4°C for 15 min ...
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bioRxiv - Neuroscience 2022Quote: ... Cells were lysed after 72 h (2+1 DIV for differentiations) using the Dual Luciferase Reporter kit (Promega, E1960) and luciferase activity was measured using a Victor3 (Perkin Elmer ...
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bioRxiv - Microbiology 2021Quote: ... A partial trypsin digestion was performed to release proteins from the beads by using 80 μL of 2 M urea/50 mM Tris containing 1 mM DTT and 0.4 μg trypsin (Mass Spectrometry Grade, Promega) for 1 h at 25 C ...
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bioRxiv - Developmental Biology 2019Quote: ... 1 mini tablet of Complete EDTA-free protease inhibitor and 2 µl RNAse inhibitor (RNAsin Plus RNase Inhibitor, Promega). RNA was extracted using the RNASpin Mini kit (GE Healthcare ...
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Blunted Fas signaling favors RIPK1-driven neutrophil necroptosis in critically ill COVID-19 patientsbioRxiv - Immunology 2021Quote: Supernatants were incubated 1:2 with the substrate solution of the CytoTox 96® Non-Radioactive Cytotoxicity Assay (Promega) for 30 min in the dark in a 96-well plate (Greiner) ...
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bioRxiv - Biochemistry 2020Quote: ... before being washed and resuspended in 2 M urea and trypsinized overnight with 0.5 μg μl−1 sequencing grade trypsin (Promega). Tryptic peptides were eluted off ...
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bioRxiv - Molecular Biology 2022Quote: ... Semiquantitative PCR reactions were carried out with 1–2 μL of cDNA and 0.2 μL of GoTaq DNA Polymerase (Promega) in a total reaction volume of 30 μL according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2022Quote: ... to a final urea concentration of 2 M for overnight Trypsin/Lys-C digestion at 35°C (1:100 protease:substrate ratio, Mass Spectrometry grade, Promega Corporation ...
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bioRxiv - Neuroscience 2022Quote: ... HEK cells were transfected with the targeting plasmid and two helper plasmids (psPAX2 and pMD2.G) at a 2:1.5:1 molar ratio using ProFection (Promega). Two to three days later ...
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bioRxiv - Cell Biology 2022Quote: ... The gel pieces were rehydrated in trypsin solution (50 mM ABC pH 8.0, 1 or 2 µg trypsin per sample for proteome or BioID respectively, Promega) overnight at 37 °C ...
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bioRxiv - Neuroscience 2023Quote: ... Then the beads were incubated with 80 μL of 2 M urea in 50 mM Tris-HCL containing 1 mM DTT and 0.4 μg trypsin (Promega) at 25 °C for 1 hour while shaking at 1,000 rpm ...
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bioRxiv - Biochemistry 2023Quote: ... Beads were resuspended in 500 µL of 100 mM Tris-HCl pH 8.0 containing 2 mM CaCl2 and digested at 37 °C for 1 h with 0.2 μg of trypsin/LysC (#V5073 Promega). The samples were then loaded onto homemade SepPak C18 Tips packed by stacking three AttractSPE disk (#SPE-Disks-Bio-C18–100.47.20 ...
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bioRxiv - Cell Biology 2023Quote: ... various concentrations of the compounds were mixed with 1 - 2 nM sensor and 1,000-fold diluted furimazine (Nano-Glo Luciferase Assay Substrate, Promega) in 100 μL of buffer (50 mM HEPES ...
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bioRxiv - Immunology 2023Quote: Transient transfection was performed using 1 or 2 µg plasmid DNA and 3.5 or 7 µl FuGENE HD transfection reagent (Promega) per 5x 105 cells ...
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bioRxiv - Bioengineering 2022Quote: Induction of apoptosis after 24 h of doxorubicin treatment was quantified by analyzing Caspase 3/7 activity using the Caspase-Glo® 3/7 3D assay (Promega, Madison, WI). The assay protocol was followed from the manufacturer’s website ...
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bioRxiv - Molecular Biology 2023Quote: ... diluted with ABC to a final concentration of 1 M urea and digested using trypsin (Promega, V5113; 3 μg/mL, 37°C, 16 h). After digestion ...
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bioRxiv - Immunology 2019Quote: ... BRET1 between hRLuc8 and mVenus was measured 5 min after addition of 5 µM coelenterazine H (Promega). BRET1 readings were collected using a Mithras LB940 reader (Berthold) ...
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bioRxiv - Biochemistry 2021Quote: ... and then cells were pre-incubated 5 minutes at 37°C with 5 μM coelenterazine h (Promega) before adding ligands diluted in PBS supplemented with 0.9 mM CaCl2 and 0.5 mM MgCl2 ...
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bioRxiv - Biochemistry 2021Quote: ... and then cells are pre-incubated 5 minutes at 37°C with 5 μM coelenterazine h (Promega) before adding ligands diluted in PBS supplemented with 0.9 mM CaCl2 and 0.5 mM MgCl2 ...
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bioRxiv - Cancer Biology 2021Quote: ... The 5’ UTR of mouse 5’-tRFCys targets or non-target Gapdh were cloned into psiCHECK2 (Promega). Synthetic 5’-tRF-Cys antisense or a scrambled control sequence embedded in the tough decoy backbone (Haraguchi et al. ...
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bioRxiv - Biophysics 2022Quote: ... Blots were incubated with secondary antibodies for 1h at room-temperature (1:10000 in 5% skim-milk powder, anti-rb-HRP-conjugate (Promgea, W401B)/anti-ms-HRP-conjugate (Promega, W402B)) ...
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bioRxiv - Genomics 2022Quote: ... Membranes were washed in TBST and probed with anti-mouse HRP secondary antibody (Promega; 1:2,000 in 5% BSA in TBST) for 1 hour at room temperature followed by development in ECL (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2023Quote: ... Nanoluciferase bioluminescence was measured from the Hyp1-Nluc parasites by lysing the parasite culture 1:5 with 1x Nano-Glo Lysis Buffer (Promega, USA), and the addition of 1:1000 Nano-Glo (Promega) ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... Membranes were washed in TBST and probed with anti-mouse HRP secondary antibody (Promega; 1:2000 in 5% BSA in TBST) for 1 h at room temperature followed by development in ECL (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2020Quote: ... Transfection was performed in the 6-well culture plate using FuGene-HD (Promega, E2311) following manufacturer’s instructions ...
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bioRxiv - Cell Biology 2022Quote: Retrovirus was produced in Phoenix Amphotropic cells by transfection with FUGENE 6 (Promega E2692) of pLPC-TRF2 or pLPC-Empty ...
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bioRxiv - Cell Biology 2021Quote: ... and a pcDNA5/FRT plasmid of interest using the Fugene 6 transfection kit (Promega) or Lipofectamine 2000 (Invitrogen) ...
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bioRxiv - Cancer Biology 2022Quote: ... Plates were collected at day 6 and measured by CellTiter-Glo (Promega #PR-G7573) following the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2020Quote: HEK 293T cells grown on 10 cm dish were transfected using FuGENE 6 (Promega) with the transfer vector pxPAX2 (Addgene #12260 ...
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bioRxiv - Biochemistry 2019Quote: ... Cells were transiently transfected with 0.4 µg DNA using FuGENE 6 transfection reagent (Promega) in a 1:3 µg DNA:µL FuGENE ratio 48 h before electrophysiology measurements were performed.
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bioRxiv - Immunology 2020Quote: ... China) or the D614G variant (constructed by site-directed mutagenesis) using FuGENE 6 (Promega). The next day ...
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bioRxiv - Developmental Biology 2020Quote: ... For the immunolabeling experiment HeLa cells were transfected with plasmids using FuGENE 6 (Promega). For streptavidin pull-down assays from HEK293T cells ...
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bioRxiv - Genetics 2021Quote: ... Plasmid transfection of U2OS cells was carried out using FuGENE 6 Transfection Reagent (Promega) according to the manufacturer’s protocol ...
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bioRxiv - Immunology 2020Quote: ... Transfection with plasmid was performed following standard protocols for Fu gene 6 (Promega, #E2691). Cells were selected for stable expression using Geneticin (G418 sulfate ...
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bioRxiv - Biophysics 2021Quote: ... U2OS cells transfection with engineered lentiCRISPRv2 were done using FuGENE 6 transfection reagent (Promega). Positive clones were selected using puromycin and subsequently isolated.
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bioRxiv - Cell Biology 2022Quote: ... Bacmids were transfected to Sf9 insect cells by lipofection (FuGENE 6 Transfection Reagent, Promega), to generate baculovirus ...
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bioRxiv - Cell Biology 2023Quote: ... Plasmid transfection of U2OS cells was carried out using FuGENE 6 Transfection Reagent (Promega) according to the manufacturer’s protocol.
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bioRxiv - Cancer Biology 2023Quote: ... Plates were collected at day 6 and measured by CellTiter-Glo (Promega #PR-G7573). Cell viability values were analyzed following blank cell deductions and normalization to vehicle readings ...
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bioRxiv - Microbiology 2023Quote: ... cells were transfected with MX2-mScarlet vector using FuGENE 6 transfection reagent (#E2691, Promega) following the manufacturers protocol ...
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bioRxiv - Biochemistry 2024Quote: ... 24 hours after transient transfection with mouse or human ENPP3 via Fugene 6 (Promega), the media was gently removed and replaced with serum-free DMEM supplemented with 1% insulin-transferrin-selenium-sodium pyruvate (ThermoFisher ...
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bioRxiv - Cell Biology 2024Quote: ... cells were transfected with TPMT-FLAG or TPMT A80P-FLAG with FuGENE 6 (Promega). 24 h post transfection ...
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bioRxiv - Molecular Biology 2024Quote: ... 50 ng/μl of mRNA and 6 ng/μl QuantiLum Recombinant Luciferase protein (Promega) were coinjected ...