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Citations for Promega :
1201 - 1250 of 2167 citations for 3 Iodo 2 6 dimethyl benzoic acid since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2020Quote: ... DNA was extracted from these bacterial suspensions using a Maxwell® 16 automated nucleic acid purification system and the Maxwell® tissue DNA purification kit following the manufacturer’s instructions (Promega, UK). Purified DNA extracts were treated with RNase (New England BioLabs ...
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bioRxiv - Molecular Biology 2020Quote: ... the reaction mix in a final volume of 83 µl has been prepared by adding 21 µl of the Amino Acid Mixture (for a final concentration of 0.13 mM) and 80 units of RNasin (Promega Ref. number N2511) to 60 µl of RRL ...
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bioRxiv - Evolutionary Biology 2021Quote: Viral RNA (vRNA) was extracted from 100 μl of VTM using the Viral Total Nucleic Acid Purification kit (Promega, Madison, WI, USA) on a Maxwell RSC 48 instrument and eluted in 50 μL of nuclease-free H2O.
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bioRxiv - Microbiology 2023Quote: Total nucleic acid extraction from swab and tissue samples was performed using ReliaPrep™ Viral TNA Miniprep System (Promega, Madison, WI, USA). Swab samples were simply inverted in the tube and briefly centrifuged prior to extraction ...
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bioRxiv - Pathology 2024Quote: ... amniotic fluid and eluates from swab samples) using the Viral Total Nucleic Acid Purification Kit for the Maxwell RSC instrument (Promega, Madison WI) with the following modifications ...
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bioRxiv - Microbiology 2024Quote: ... Total RNA was extracted using the Maxwell 16 LEV simply RNA tissue kit in combination with a Maxwell nucleic acid extraction robot (Promega, WI, USA) for rabbit caecal content ...
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bioRxiv - Cell Biology 2024Quote: ... five µg protein were reduced by 2.5 mM dithiothreitol for 30 min at 37°C and alkylated with 10 mM iodoacetic acid for 30 min at 37°C in the dark before digestion by trypsin (Promega, Walldorf, Germany) in a protein to enzyme ratio 25:1 overnight at 37°C ...
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bioRxiv - Plant Biology 2020Quote: ... 3×HA-fused CabLCV MP and NSP proteins were independently expressed using the TNT system (Promega) according to the manufacturer’s recommendations ...
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bioRxiv - Molecular Biology 2020Quote: Apoptosis assays were performed using Caspase-Glo 3/7 reagent according to manufacturer’s instructions (Promega #0000239042). Cell viability assays were performed using WST-1 cell viability reagent according to manufacturer’s instructions (Sigma #18993700) ...
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bioRxiv - Cell Biology 2021Quote: ... Caspase activation was measured using the Caspase Glo 3/7 Assay Kit (Promega, Madison, WI, USA) according to manufacturer’s instructions.
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bioRxiv - Biochemistry 2020Quote: ... cell metabolic activity or caspase 3/7 activity was measured using the CellTiter-Glo assay (Promega) and Caspase-Glo 3/7 assay (Promega) ...
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bioRxiv - Cell Biology 2021Quote: ... Cells were transfected with Galectin-3-GFP plasmid (0.5 µg) using FuGene HD (Promega, Madison, WI) with a ratio of 3:1 for 24 h at 37 °C in 5% CO2 ...
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bioRxiv - Evolutionary Biology 2022Quote: ... Tryptic digestion was performed for 3 h with 0.1 μg trypsin (Promega Sequencing Grade; Madison, WI) per sample ...
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bioRxiv - Cell Biology 2022Quote: ... apoptosis was quantified using the Apo-ONE homogeneous caspase-3/7 assay kit (Promega, Madison, USA) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2020Quote: ... WT or mutant promoter of human Rpl28 gene was cloned into pGL-3-basic (Promega, E1751). The number is relative to transcriptional start site ...
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bioRxiv - Biophysics 2020Quote: ... 3 μL of translation reaction was added to 30 μL Bright-Glo Luciferase assay reagent (Promega) and the resulting luminescence was measured in the illuminometer over a spectral wavelength of 350–650 nm and an integration time of 10 s at room temperature ...
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bioRxiv - Cancer Biology 2020Quote: ... Luminescence was determined after 3 days of culture using the GloMax® microplate reader (GM3000, Promega) and normalized with seeding control plate.
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bioRxiv - Microbiology 2021Quote: ... Caspase activity was determined using the Caspase-Glo 3/7 assay system (Promega, Madison, WI, USA) according to the manufacturer’s instruction ...
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bioRxiv - Microbiology 2021Quote: ... Infectivity was determined after 3 days by luminescence following addition of BrightGlo reagent (Promega PR-E2620). IC50 were calculated by nonlinear regression analysis using GraphPad Prism (version 9.0 ...
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bioRxiv - Microbiology 2022Quote: Caspase 3/7 activity was measured using the Caspase-Glo assay kit (Promega, Madison, WI, USA), according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2022Quote: Caspase 3/7 activity and cell viability were determined by ApoTox-Glo assay kit (G6321, Promega). Briefly ...
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bioRxiv - Microbiology 2022Quote: ... the second set of plates was analyzed by adding CellTiter-Glo 2.0 Reagent (Promega, G9242/3) and measuring luminescence on a GloMax Discover System (Promega) ...
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bioRxiv - Molecular Biology 2022Quote: The 3’UTR of the genes of interest was cloned in the pSI-Check2 vector (Promega) using the primers listed in Supplementary Table S6 ...
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bioRxiv - Neuroscience 2024Quote: ... Caspase activity was measured with the Caspase-Glo® 3/7 (Promega Corp., Fitchburg, WI, USA) assay kit ...
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bioRxiv - Immunology 2024Quote: ... 3×106 ADCC and ADCP Bioassay Effector Cells expressing the human FcγRIIIa or FcγRIIa receptors (Promega) were added per well and the cells were incubated in at 37°C with 5% CO2 for 6 hours ...
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bioRxiv - Cancer Biology 2023Quote: ... Caspase-3 and −7 activity was measured following three days of treatment (Caspase-Glo #G8093, Promega), while cell viability was obtained after six days of treatment (CellTiter-Glo 3D #G9681) ...
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bioRxiv - Microbiology 2023Quote: ... following tryptic digest at 37 °C for 3 h in the same buffer with trypsin (Promega) in a 1:30 ratio (w/w ...
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bioRxiv - Microbiology 2023Quote: ... the cells were co-transfected using Fugene HD transfecting reagent at a 1:3 ratio (Promega) along with pRL-TK Renilla (Renilla Luciferase ...
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bioRxiv - Molecular Biology 2023Quote: ... Tryptic digestion was performed for 3 h with 0.1 μg trypsin at 37°C (Promega, V5113) per sample ...
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bioRxiv - Cell Biology 2023Quote: NRK cells were transfected with IP3R-3 siRNA (see below) using Transfast (Promega Corp.; Madison WI) using manufacturer’s instructions ...
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bioRxiv - Plant Biology 2024Quote: RNAs including 5’ and 3’ UTRs were synthesized in vitro using T7 RNA polymerase kit (Promega). cDNA from A ...
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bioRxiv - Cell Biology 2024Quote: ... were measured 48hr post-transfection using the Proteasome-Glo 3-Substrate Cell-Based Assay System (Promega) following the manufacturer’s instructions.
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Downregulation of Let-7 miRNA promotes Tc17 differentiation and emphysema via de-repression of RORγtbioRxiv - Immunology 2024Quote: Genomic fragment containing the murine Rorc 3’UTR was cloned into psiCHECK2 luciferase reporter plasmid (Promega). This construct was also used to generate the let-7 ʻseed’ deletion mutant derivative using the QuikChange Multi Site Mutagenesis Kit (catalog 200514-5 ...
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bioRxiv - Cell Biology 2020Quote: ... The AIO plasmid and the template plasmid were transfected into HAP1 STG-p62 (for mScarlet-AID-NBR1) or HAP1 WT (for GFP-AID-NBR1) cells using FuGene 6 transfection reagent (Promega, cat# e2691). 48h later the cells were sorted for mScarlet fluorescence in bulk ...
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bioRxiv - Microbiology 2020Quote: ... The env gene clones together with the env-deficient HIV-1 plasmid DNA (SG3Δenv) were contransfected into HEK293T cells (ATCC, Manassas, VA, USA) using the FuGene 6 transfection reagent (Promega, Madison, WI, USA). Two days after transfection ...
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bioRxiv - Biochemistry 2022Quote: ... (complete media) were co-transfected with our library of retinopathy variants and the Bxb1 recombinase expression vector using Fugene 6 (Promega, Madison, WI). Doxycycline (2 μg/mL ...
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bioRxiv - Immunology 2022Quote: ... L981F. Pseudovirions were produced in HEK 293T/17 cells (ATCC cat. no. CRL-11268) by transfection using Fugene 6 (Promega, Catalog #E2692). Pseudovirions for 293T/ACE2 infection were produced by co-transfection with a lentiviral backbone (pCMV ΔR8.2 ...
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bioRxiv - Biochemistry 2020Quote: ... The mutation was confirmed by full-length Spike gene sequencing. Pseudovirions were produced in HEK 293T/17 cells (ATCC cat. no. CRL-11268) by transfection using Fugene 6 (Promega Cat#E2692) and a combination of Spike plasmid ...
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bioRxiv - Cancer Biology 2021Quote: ... Plasmids were packaged with the third-generation lentiviral packaging system (VSV-G, pMDLg, and pRSV-REV) and expressed in HEK293T cells via transient transfection with FuGENE 6 (Promega, Madison, WI). Virus-containing supernatants were harvested after 24 and 48 hours ...
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bioRxiv - Immunology 2021Quote: ... The mutation was confirmed by full-length spike gene sequencing. Pseudovirions were produced in HEK 293T/17 cells (ATCC cat. no. CRL-11268) by transfection using Fugene 6 (Promega, Catalog #E2692). Pseudovirions for 293T/ACE2 infection were produced by co-transfection with a lentiviral backbone (pCMV ΔR8.2 ...
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bioRxiv - Immunology 2021Quote: ... All mutations were confirmed by full-length Spike gene sequencing. Pseudovirions were produced in HEK 293T/17 cells (ATCC cat. no. CRL-11268) by transfection using Fugene 6 (Promega Cat#E2692) and a combination of Spike plasmid ...
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bioRxiv - Cancer Biology 2022Quote: ... 1000 and 5000 µM) or remained untreated for 6 days and viability was analyzed using the CellTiter-Glo Luminescent Cell Viability Assay (Promega, Dübendorf, Switzerland) to determine the adenosine triphosphate (ATP ...
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bioRxiv - Biochemistry 2022Quote: ... cells grown in 10 cm dishes in complete media were co-transfected with our library of cystic fibrosis variants and the Bxb1 recombinase expression vectors using Fugene 6 (Promega, Madison, WI). Doxycycline (2 μg/mL ...
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bioRxiv - Immunology 2024Quote: ... 6 μg of each vector was transfected into tumor cells plated on a 6-well plate using FuGENE6 transfection reagent (Promega, cat. E2691) and OPTI-MEM (GIBCO ...
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bioRxiv - Biochemistry 2024Quote: Total RNA was isolated from the cells of 6-well plates treated as above using the Maxwell RSC simplyRNA kit and the Maxwell RSC Instrument (both Promega, Southampton, UK) according to manufacturer’s instructions ...
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bioRxiv - Microbiology 2024Quote: ... and 5 µg of the pHR’CMV-Luc (luciferase reporter) plasmid in 10-cm dishes containing 50-80% confluent 293T cells in 10% FBS/DMEM using Fugene 6 (Promega, Madison, WI). 48 h after transfections ...
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bioRxiv - Biophysics 2024Quote: ... For PMP22 experiments: 24 hrs later cells were transfected with 15 ug of pSF PMP22 N41Q-myc using Fugene 6 (Promega cat # E2691) following the manufacturers protocol ...
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bioRxiv - Cancer Biology 2024Quote: ... The extracted plasmid was transfected with packing plasmid (psPAX2) and envelope VSV-G plasmid (pMD2.G) using FuGENE® 6 Transfection Reagent (Promega, E2691). The following lentivirus harvest ...
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bioRxiv - Microbiology 2022Quote: ... The viral RNAs were extracted from the homogenized lung tissue samples (10% w/v) and nasal wash samples using the Maxwell® RSC Viral Total Nucleic Acid Purification Kit (Promega, Madison, WI). The whole genomes of SARS-CoV-2 used in this research were amplified using a modified ARTIC protocol with several primers replaced or added (57 ...
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bioRxiv - Evolutionary Biology 2020Quote: ... or pB.2-eIF2α-S51A plasmids by use of FuGENE6 (Promega) according to the manufacturer’s protocol ...