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Citations for Promega :
1151 - 1200 of 3977 citations for Human Immunodeficiency Virus GP120 Protein HIV 1 Clade A KNH1144 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2024Quote: ... Proteins on the beads were digested by adding 200 ng trypsin/Lys-C mix (Promega) at 37 °C overnight ...
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bioRxiv - Cell Biology 2024Quote: ... cells transfected with HALO-fusion protein were supplemented with 100 nM TMR HALO ligand (Promega). Cells were fixed at RT for 20 min in 3.7% formaldehyde (FA ...
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bioRxiv - Molecular Biology 2024Quote: ... Proteins were digested by adding 5 μL of sequencing grade trypsin (0.1 mg/mL) (Promega) for three h at 37 °C ...
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bioRxiv - Biochemistry 2024Quote: ... For protein synthesis in reticulocyte lysate the TNT® Quick Coupled Transcription/Translation System (Promega) was used and performed according to the manufacture’s protocol employing 1 mM unlabeled methionine ...
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bioRxiv - Microbiology 2024Quote: ... Proteins were then digested using 250 ng of trypsin enzyme (Sequencing Grade Modified Trypsin, Promega) and incubation for 1h at 37°C ...
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bioRxiv - Microbiology 2021Quote: DNA extraction of samples was performed according to an adapted version of the “DNA purification from human feces using the Maxwell® RSC instrument and the Maxwell® RSC PureFood GMO and Authentication Kit” developed by Promega (Promega Corporation ...
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bioRxiv - Microbiology 2020Quote: ... 3’-untranslated region (3’-UTR) fragment of IκBα (human/rat) was inserted into the pGL3-basic vector (firefly luciferase; Promega, Madison, WI, USA), which was obtained from General Biosystems (Anhui ...
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bioRxiv - Molecular Biology 2021Quote: ... RPGR wild-type minigene construct was generated (Figure 2A) by amplification from a pool of human female genomic DNAs (Promega, Milan, Italy) using the following primers:
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bioRxiv - Biophysics 2022Quote: pHalo-PPARγ2 expresses human PPARγ isoform 2 fused to HaloTag in the N-terminus under a CMVd1 promoter (Promega ORF clone #FHC08305). PPARγ2 mutants were generated by nucleotide substitution using the QuikChange II XL Site Directed Mutagenesis Kit (Stratagene ...
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bioRxiv - Neuroscience 2023Quote: ... Sections were then incubated for 24-48hrs in the blocking solution containing chicken anti-human NT-4/5 antibody (10 mg/ ml, Promega, Madison, WI) or rabbit anti-human NT-4/5 (1:1000 ...
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bioRxiv - Immunology 2024Quote: ... qPCR targeting the β-globin gene was performed for each sample and evaluated alongside a β-globin calibration curve (human gDNA, cat# G1521, Promega).
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bioRxiv - Microbiology 2022Quote: The extent of ADCC activation induced by human γ-globulins was evaluated with the use of an ADCC Reporter Bioassay (Core Kit; Promega, G7010) and the EnSpireMultimode Plate Reader (PerkinElmer) ...
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bioRxiv - Cell Biology 2023Quote: ... the corresponding promoter regions of the human KLF2 and KLF4 genes were amplified using human genomic DNA as a template and cloned into the BglII site of the GL4 basic vector (Promega, Fitchburg, WI). Point mutants of the vectors were constructed by site-directed mutagenesis using a QuikChange Site-Directed Mutagenesis kit (Stratagene ...
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bioRxiv - Cell Biology 2019Quote: ... diluted 1/500 in PBS-BSA 1% and 0.4 U.mL-1 RNAsin (Promega). We visualized and quantified 5-FUrd incorporation by using high content imaging device (OPERETTA ...
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bioRxiv - Cancer Biology 2021Quote: ... short tandem repeat analysis verified the identity of mouse (Bioassay Methods Group, National Institute of Standards and Technology) and human (Promega GenePrint 10 System) cells.
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bioRxiv - Microbiology 2019Quote: ... These stock solutions were then diluted to the indicated concentrations in sequencing-grade water and 10 ng commercial human DNA (Promega Corp cat#G3041) was added to all samples ...
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bioRxiv - Synthetic Biology 2022Quote: Cytotoxicity assay was performed on HCT116 human colon cells (ATCC, #CCL-247™) using CellTiter 96® AQueous One Solution Cell Proliferation Assay (Promega, #G3580) which is a colorimetric method based on MTS (3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2- (4-sulfophenyl)-2H-tetrazolium ...
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bioRxiv - Systems Biology 2023Quote: ... Peptide loading was assessed by comparing the intensity of the total ion current to 200 ng of a human K562 whole cell lysate standard (Promega, Madison, Wisconsin, USA) prepared at 0.2 μg/μL.
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Development of a genetically encoded sensor for probing endogenous nociceptin opioid peptide releasebioRxiv - Neuroscience 2024Quote: HEK293 cells growing at 70% confluency in a 10-cm dish were transfected with wild-type human NOPR or NOPLight (3 μg DNA) and GloSensor-20F (Promega, 2.5 μg DNA) using 12 μL Lipofectamine 2000 (Thermo Fisher ...
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bioRxiv - Microbiology 2020Quote: ... Reduced and alkylated proteins were in-gel digested with 100 ng trypsin (modified sequencing grade, Promega) overnight at 37°C ...
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bioRxiv - Cell Biology 2020Quote: ... GST fusion proteins were isolated by binding to glutathione magnetic beads (MagneGST™ Glutathione Particles, Promega) for 30 min at room temperature on a rotating wheel ...
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bioRxiv - Plant Biology 2020Quote: ... 3×HA-fused CabLCV MP and NSP proteins were independently expressed using the TNT system (Promega) according to the manufacturer’s recommendations ...
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bioRxiv - Molecular Biology 2020Quote: ... Proteins were digested with Lys-C (Wako Chemicals) for 6 hours and trypsin (Trypsin Gold, Promega) overnight.
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bioRxiv - Cell Biology 2022Quote: ... Pellets were lysed and bait proteins in the lysates were bound to HaloLink Magnetic Beads (Promega) according to manufacturer’s instructions ...
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bioRxiv - Immunology 2022Quote: ... and proteins were subjected to overnight digestion with mass spec grade Trypsin/Lys-C mix (Promega). Following digestion ...
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bioRxiv - Molecular Biology 2021Quote: ... Cells were transfected with plasmids encoding fluorescently tagged proteins using FuGENE® HD Transfection Reagent (Promega), following manufacturer’s protocol and assayed 1-3 days after transfection ...
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bioRxiv - Systems Biology 2019Quote: ... Enzymatic protein hydrolysis was initiated by the addition of trypsin (Sequencing Grade Modified Trypsin, Frozen, Promega) at a ratio of 1:50 ...
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bioRxiv - Microbiology 2019Quote: ... proteins were incubated overnight at 37 °C with 500bng trypsin (Trypsin Gold Mass Spectrometry Grade, Promega). Peptides were recovered by centrifugation.
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bioRxiv - Cell Biology 2019Quote: ... Proteins were digested in-gel with trypsin (0.17 μg in 10 μl 40 mM NH4HCO3, Promega) at 37°C over night ...
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bioRxiv - Molecular Biology 2019Quote: ... Activity was normalized to total protein following quantification using the Dual Luciferase Reporter Assay System (Promega).
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bioRxiv - Biochemistry 2020Quote: ... proteins were acetone precipitated and digested using LysC (Wako sequencing grade) and trypsin (Promega sequencing grade), as described in (Buczak et al. ...
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bioRxiv - Plant Biology 2021Quote: ... Halo–GmRAV fusion protein was expressed using the TNT SP6 Coupled Wheat Germ Extract System (Promega) following the manufacturer’s specifications for expression ...
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bioRxiv - Biochemistry 2022Quote: ... [35S]-labeled proteins were synthesized in vitro via a coupled transcription/translation rabbit reticulocyte kit (Promega) incubated at 30°C for 90 min with [35S]Met/Cys metabolic labeling reagent (MP Biomedical ...
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bioRxiv - Biochemistry 2021Quote: ... and proteins were subjected to overnight digestion with Mass Spec Grade Trypsin/Lys-C mix (Promega). The beads were then pulled down and the solutions containing the digested peptides were desalted using AssayMap C18 cartridges mounted on an AssayMap Bravo liquid handling system (Agilent Technologies ...
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bioRxiv - Microbiology 2020Quote: ... 150 μg of protein from each sample digested overnight/37 °C with 1.5 μg trypsin (Promega), followed by addition of further 1.5 μg of trypsin and digestion for an additional 4-hrs ...
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bioRxiv - Cell Biology 2021Quote: ... and proteins were expressed with a TNT Quick Coupled Transcription/Translation System (Promega, Madison, WI, USA) according to the manufacturer’s protocol ...
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bioRxiv - Biochemistry 2021Quote: Mitochondrial 35S-labeled precursor proteins were synthesized using the TNT SP6-coupled transcription/translation kit (Promega) and plasmid-vectors pSP64 containing the genes of interest ...
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bioRxiv - Biophysics 2021Quote: Analysis of G-protein recruitment was performed by using a modified protocol of NanoBiT system (Promega) assay described previously5 ...
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bioRxiv - Cell Biology 2022Quote: ... 5 mM iodoacetamide (IAA) and proteins were eluted by digestion with 5 µg/ml trypsin (Promega). Eluted proteins were fully digested overnight ...
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bioRxiv - Neuroscience 2019Quote: ... 100 µg of protein was then added to 100 µl of prewashed HaloLink resin (Promega G1914), which was prepared by washing and pelleting for 2m at 800xg 3x in wash buffer (100 mM Tris pH 7.5 ...
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bioRxiv - Molecular Biology 2019Quote: Dried protein samples were digested in 200 mM EPPS buffer pH 8.5 with trypsin (Promega #V5111). Digests contained 2% acetonitrile (v/v ...
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Genome modularization reveals overlapped gene topology is necessary for efficient viral reproductionbioRxiv - Synthetic Biology 2020Quote: ... 30 μg of protein was taken for digestion with trypsin (2% (w/w)) (Promega, United States) and left overnight at 37°C (∼18-hours) ...
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bioRxiv - Developmental Biology 2021Quote: Proteins were synthesized with the TnT SP6 Quick Coupled Transcription/Translation System (Promega, Madison, Wisconsin, USA).
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bioRxiv - Microbiology 2019Quote: ... The gene that encodes the HaloTag protein was optimized for Magnetospirillum codons with permission from Promega Corporation (Madison ...
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bioRxiv - Microbiology 2021Quote: ... Proteins were subsequently digested off the beads by addition of 0.4 µg Trypsin/LysC (Promega, #V5072) for 1 h while shaking in a thermoshaker at 37°C ...
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bioRxiv - Cell Biology 2019Quote: ... the bead-bound protein was incubated with either 6.7 µM HaloTag-AlexaFluor660 or HaloTag-TMR (Promega), or SNAP-Surface Alex Fluor 647 (NEB) ...
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bioRxiv - Physiology 2019Quote: ... SDS was removed by chloroform/methanol precipitation and the proteins were proteolytically digested with trypsin (Promega) and purified using OMIX C18 Mini-Bed tips (Agilent Technologies ...
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bioRxiv - Developmental Biology 2020Quote: Tryptic digestion of bead-bound protein was performed in 5% w/w trypsin (Promega, cat. #V5280), 50mM triethylammonium bicarbonate buffer at 37 °C overnight ...
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bioRxiv - Developmental Biology 2020Quote: Unlabelled in vitro translated protein was produced using rabbit reticulocyte lysates according to manufacturer’s protocol (Promega).
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bioRxiv - Biochemistry 2022Quote: ... aliquots of EPO proteins were digested respectively using a combination of trypsin and Glu-C (Promega), or Glu-C (Promega) ...