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Citations for Promega :
1151 - 1200 of 2410 citations for Dengue Virus Serotype 3 Envelope Protein Human Fc Tag HEK293 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2023Quote: ... Luminescence from HiBiT-tagged proteins was measured with GloMax Navigator (Promega). Luminescence from CMs and lysates of nontransfected cells were used for background correction.
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bioRxiv - Bioengineering 2023Quote: ... Proteins were digested over night by the addition of trypsin (Promega) and samples were acidified by using formic acid according to [29] ...
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bioRxiv - Microbiology 2023Quote: ... proteins were digested in-gel using modified trypsin (sequencing purity, Promega), as previously described [51] ...
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bioRxiv - Microbiology 2023Quote: ... Proteins were digested on-column with 0.75 µg of trypsin (Promega) in 50 mM TEAB pH 8.5 overnight at 37°C in a humidified incubator ...
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bioRxiv - Cancer Biology 2023Quote: ... 30μg of proteins were digested with 1µg Trypsin sequencing grade (Promega) for 14h at 37°C ...
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bioRxiv - Cell Biology 2023Quote: ... Proteins were then digested with sequencing grade trypsin (Promega, Madison, WI) at 37 °C overnight ...
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bioRxiv - Cancer Biology 2022Quote: ... Proteins were digested with endoproteinase LysC (Wako Chemicals) and trypsin (Promega) overnight at 37°C ...
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bioRxiv - Plant Biology 2023Quote: ... proteins were digested in-gel using trypsin (modified, sequencing purity, Promega), as previously described [88] ...
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bioRxiv - Systems Biology 2023Quote: ... Proteins were digested by addition of 1 µg of Trypsin (Promega) and incubated overnight at 37 °C at 600 rpm on a thermoshaker ...
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bioRxiv - Microbiology 2023Quote: ... and the protein solution was digested with 0.5 µg trypsin (Promega) overnight at room temperature ...
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bioRxiv - Plant Biology 2023Quote: ... 300 mM NaCl and protein inhibitor cocktail (Promega, Madison, Wisconsin, USA) at pH 7.4 ...
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bioRxiv - Microbiology 2023Quote: ... After 24h the cells were harvested using protein lysis buffer (Promega) and centrifuged for 10min at 13,000rpm ...
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bioRxiv - Cell Biology 2023Quote: Proteins were cleaved with the trypsin/endoproteinase LysC mixture (Promega, V5072) using the paramagnetic bead-based method (54) ...
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bioRxiv - Biochemistry 2023Quote: ... then digested with lysC/trypsin mixture (1: 50, enzyme: protein, Promega) at 37℃ overnight ...
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bioRxiv - Genomics 2023Quote: ... We added Nano-Glo Luciferase Assay Substrate and LgBiT protein (Promega) and imaged using the ImageQuant LAS4000 Imager.
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bioRxiv - Molecular Biology 2023Quote: ... Proteins were in-gel digested using 1µg trypsin (Trypsin Gold, Promega)(37) ...
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bioRxiv - Cancer Biology 2023Quote: ... proteins were digested in-gel using modified trypsin (sequencing grade, Promega), as previously described 58 ...
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bioRxiv - Molecular Biology 2023Quote: ... Proteins were eluted from beads using partial on-bead trypsination (Promega) followed by a second digestion ...
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bioRxiv - Cancer Biology 2024Quote: ... Proteins were digested overnight at 25°C with modified trypsin (Promega) using an enzyme-to-substrate ratio of 1:50 in 100 mM ammonium bicarbonate ...
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bioRxiv - Cancer Biology 2024Quote: ... Proteins were digested with sequencing-grade modified trypsin (Promega, Madison, WI) overnight at 37 °C ...
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bioRxiv - Cell Biology 2024Quote: ... Protein lysates were digested into peptides by addition of trypsin (Promega) and LysC (Wako ...
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bioRxiv - Cell Biology 2024Quote: ... Protein digestion was carried out with sequencing grade modified Trypsin (Promega) at 1/50 protease/protein (wt/wt ...
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bioRxiv - Bioengineering 2024Quote: ... Then freshly diluted trypsin dissolved in protein digestion buffer (from Promega), was added to the samples at a ratio of 1:50 of trypsin:protein and incubated overnight at 37 oC in a Thermomixer ...
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bioRxiv - Bioengineering 2024Quote: ... Proteins were subsequently digested with trypsin (sequencing grade, Promega, Madison, WI), at an enzyme/substrate ratio of 1:50 ...
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bioRxiv - Cell Biology 2024Quote: ... Proteins were digested on beads with 1 μg trypsin (Promega, UK) for 18 hours at 37 °C ...
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bioRxiv - Molecular Biology 2024Quote: ... The proteins were on-bead digested with 1 µg trypsin (Promega) for 4 hours at 37 °C under agitation ...
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bioRxiv - Molecular Biology 2024Quote: ... TNT® SP6 High-Yield Wheat Germ Protein Expression System (Promega) was used ...
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bioRxiv - Cancer Biology 2024Quote: ... Protein digestion was carried out with sequencing grade modified Trypsin (Promega) at 1/50 protease/protein (wt/wt ...
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bioRxiv - Cell Biology 2024Quote: ... proteins were digested in-gel using trypsin (modified, sequencing purity, Promega) as previously described (Casabona et al. ...
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bioRxiv - Cell Biology 2024Quote: ... Proteins were cleaved with the trypsin/endoproteinase LysC mixture (Promega, V5072) using the paramagnetic beads-based method41 ...
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bioRxiv - Neuroscience 2024Quote: ... 50 μg of protein per sample was digested with trypsin (Promega) overnight at 37°C using S-TRAP micro cartridges (Protifi LLC,) ...
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bioRxiv - Molecular Biology 2021Quote: ... Cells were transfected with a control Flag or HA-GSK-3βWT human plasmid using Fugene 6 (Promega #E2693) in a 2:1 ratio and incubated for 3 hours in a CO2 incubator ...
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bioRxiv - Cancer Biology 2020Quote: ... T47D cells and MCF7 cells were transfected with empty or human LIP-containing pcDNA3.1 via Fugene HD (Promega) using the manufactures protocol ...
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bioRxiv - Cancer Biology 2021Quote: The upstream 376 bp region of the human LGALS1 transcriptional start site was cloned into the pGL4.23 (Promega) vector to generate the LGALS1 luciferase reporter gene (LGALS1 pGL4.23 ...
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bioRxiv - Cancer Biology 2022Quote: ... and MV4-11 (DSMZ Cat# ACC-102, RRID:CVCL_0064) human AML cell lines were identified by PCR-single-locus-technology (Promega, PowerPlex21 PCR Kit ...
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bioRxiv - Biophysics 2019Quote: The cell-viability of human neuroblastoma (SH-SY5Y) cells was measured using MTT cell proliferation assay (Promega, G4000). SH-SY5Y cells were plated in a 96-well plate followed by differentiation in Neurobasal-A ...
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bioRxiv - Biophysics 2021Quote: ... Human Gβ with a C-terminal 15-amino-acid polypeptide linker followed by a HiBiT (peptide 86, Promega) and human Gγ were cloned into pFastBac vector ...
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bioRxiv - Neuroscience 2024Quote: ... human CaV1.3 cDNA (accession number NM_001128840.2) was de-novo synthesized and assembled into a HaloTag vector (Promega G7721) using restriction cloning ...
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bioRxiv - Microbiology 2023Quote: ... Standard curves were generated using purified genomic stocks (HSV-1 bacterial artificial chromosome and human genome Promega #G1471). Absolute copy number of genomic stocks was determined using droplet digital PCR (Biorad QX600) ...
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bioRxiv - Molecular Biology 2020Quote: Apoptosis assays were performed using Caspase-Glo 3/7 reagent according to manufacturer’s instructions (Promega #0000239042). Cell viability assays were performed using WST-1 cell viability reagent according to manufacturer’s instructions (Sigma #18993700) ...
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bioRxiv - Neuroscience 2019Quote: ... were transfected with FLAG-tagged Nlgn1 or GFP-tagged NGL-3 using Fugene6 (Promega, Cat #E2691). Transfected HEK293T cells were mechanically dissociated 24 hr after transfection and co-cultured with DIV10 cortical neurons prepared from C57BL/6J Sorcs1flox/flox mice for 16 hr.
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bioRxiv - Physiology 2019Quote: ... Plasmid DNA (6 µg) was mixed (1 : 3 ratio) with transfection reagent (Fugene 6; Promega, UK) in reduced serum media (OptiMEM ...
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bioRxiv - Developmental Biology 2019Quote: ... Nano-Luc activity was measured after 3 hours using the Nano-Glo® Luciferase Assay (Promega) and imaged with the IVIS Lumina LT system ...
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bioRxiv - Cell Biology 2021Quote: ... Caspase activation was measured using the Caspase Glo 3/7 Assay Kit (Promega, Madison, WI, USA) according to manufacturer’s instructions.
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bioRxiv - Biochemistry 2020Quote: ... cell metabolic activity or caspase 3/7 activity was measured using the CellTiter-Glo assay (Promega) and Caspase-Glo 3/7 assay (Promega) ...
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bioRxiv - Cell Biology 2021Quote: ... Cells were transfected with Galectin-3-GFP plasmid (0.5 µg) using FuGene HD (Promega, Madison, WI) with a ratio of 3:1 for 24 h at 37 °C in 5% CO2 ...
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bioRxiv - Cancer Biology 2021Quote: ... wild type or mutant 3′-UTR of DKK3 was cloned into the psicheck-2 vector (Promega). HCT116 cells were transfected with and wild-type or mutant 3′-UTR-luc by using Lipofectamine 3000 ...
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bioRxiv - Evolutionary Biology 2022Quote: ... Tryptic digestion was performed for 3 h with 0.1 μg trypsin (Promega Sequencing Grade; Madison, WI) per sample ...
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bioRxiv - Cell Biology 2022Quote: ... apoptosis was quantified using the Apo-ONE homogeneous caspase-3/7 assay kit (Promega, Madison, USA) according to the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2021Quote: ... 2-3 μg of the purified RNA was in vitro translated using wheat germ extract (Promega) at 25 °C for 2 h as per the manufacturer’s instructions ...