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Citations for Promega :
1101 - 1150 of 5340 citations for ssc mir 376a 5p Real time RT PCR Detection Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2020Quote: PCRs were performed in 25 μl reaction buffer (Promega) with MgCl2 at 1.5mM final ...
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bioRxiv - Neuroscience 2020Quote: ... NanoLuc was PCR amplified from plasmid pNL.1.1 (Promega) and cloned into pUS2-MT to generate pUS2-MT-Nanoluc ...
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bioRxiv - Microbiology 2021Quote: ... PCR was performed using GoTaq G2 DNA Polymerase (Promega) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2022Quote: ... were amplified by PCR from human genomic DNA (Promega), using the primers listed in Table S1 and then inserted into the psiCHECK-2 vector ...
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bioRxiv - Neuroscience 2019Quote: ... in 1 × PCR thermo DNA polymerase reaction buffer (Promega). Following the addition of 100 pMol each 5’ and 3’ primer and MgCl2 to 4 mM ...
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bioRxiv - Neuroscience 2021Quote: ... The PCR products were cloned into pGEMT (Promega #1360) and sequenced to verify the mutations ...
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bioRxiv - Microbiology 2020Quote: ... sucrose-resistant colonies by PCR using GoTaq® (Promega) and primers listed in Table S2 ...
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bioRxiv - Cell Biology 2021Quote: ... The PCR product was subcloned into plasmid psiCHECK2 (Promega) previously digested with NotI using the In-Fusion cloning kit (Takara Bio ...
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bioRxiv - Microbiology 2021Quote: ... PCR was performed using GoTaq Green master mix (Promega), per the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2019Quote: ... PCR amplification was carried out using GoTaq polymerase (Promega) at 1.5 mM MgCl2 using the following cycling parameters ...
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bioRxiv - Genetics 2019Quote: ... A PCR reaction using GoTaq G2 DNA Polymerase (Promega) for Cep164tm1a(EUCOMM)Wtsi/+ ...
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bioRxiv - Cell Biology 2020Quote: ... PCR reactions were performed using Master Mix (2X) (Promega) and carried out at an initial denaturation step of 95 °C for 2 min ...
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bioRxiv - Microbiology 2020Quote: ... PCR screens were carried out using GoTaq Green (Promega) with the primer combinations described.
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bioRxiv - Microbiology 2020Quote: ... The resulting PCR product and pHis6-Halo (Promega Corporation) were digested with PvuI and NotI and ligated to create pVJS77 that was confirmed via sequencing (Georgia Genomics Facility ...
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bioRxiv - Biochemistry 2020Quote: PCR was performed using GoTaq Green Master Mix (Promega) as per manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2021Quote: ... PCR products were subcloned in pGEM-T-easy (Promega) and sequenced ...
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bioRxiv - Genetics 2021Quote: ... the coding sequence was PCR amplified from pFN31K (Promega) using forward ...
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bioRxiv - Cancer Biology 2020Quote: ... PCR reactions were performed using GoTaq DNA Polymerase (Promega) following manufacturer protocols ...
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bioRxiv - Genetics 2021Quote: ... PCR was performed with GoTaq DNA polymerase (Promega #M3005) using the following program ...
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bioRxiv - Genomics 2022Quote: ... PCR was performed with GoTaq G2 DNA Polymerase (Promega) in a final volume of 40 μL containing 1 μL of total genomic DNA ...
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bioRxiv - Developmental Biology 2022Quote: ... PCR products were cloned into pGL4.23 vector (Promega, E841A) digested with XhoI and HindIII (New England Biolabs ...
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bioRxiv - Microbiology 2022Quote: ... The PCR products were cloned into pJET vector (Promega) and sequenced ...
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bioRxiv - Plant Biology 2022Quote: ... PCR amplifications were undertaken using GoTaq DNA polymerase (Promega) and PCR cycles of 95 °C for 5 min ...
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bioRxiv - Microbiology 2022Quote: ... Genotyping PCRs were performed using GoTaq Green Mastermix (Promega) (Fig ...
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bioRxiv - Microbiology 2023Quote: ... PCR products were cloned into vector pGEM-T (Promega). CRISPR A and B start naturally with a 6 nt cassette consisting of adenines and guanines that we used for synchronisation of transcription (see below) ...
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bioRxiv - Microbiology 2023Quote: ... unlabeled PCR was cloned into pGEM-T Easy (Promega) and positive clones were checked by amplification with the vector-based primers M13f and M13R.
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bioRxiv - Physiology 2023Quote: ... The PCR products were subcloned to T-vector (Promega) and sequenced ...
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bioRxiv - Cell Biology 2022Quote: ... After PCR clean-up (Promega #A9281, Madison, WI, USA), A-tailing of the PCR products with Taq Polymerase was performed as ...
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bioRxiv - Developmental Biology 2023Quote: ... PCR products were subcloned in pGEM-T-easy (Promega) and sequenced ...
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bioRxiv - Microbiology 2023Quote: ... Each PCR reaction contained 0.25 µl Taq polymerase (Promega), 2.5 µl 10 µM forward and reverse primers ...
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bioRxiv - Molecular Biology 2023Quote: ... PCR was performed using GoTaq Flexi DNA Polymerase (Promega), and PCR products were migrated on agarose gels ...
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bioRxiv - Cell Biology 2023Quote: ... the PCR product was cloned into pGEMTeasy (Promega A1380). The mutagenesis of the EPIYA sites were done one after the other using the kit Quick change II site-directed mutagenesis (Aligent #200523) ...
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bioRxiv - Cell Biology 2023Quote: ... the PCR product was cloned into pGEMTeasy (Promega A1380). The cagA fragment was digested with KpnI and EagI and subcloned into the lentivirus entry vector pEF-1α/pENTR (Addgene ...
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bioRxiv - Bioengineering 2023Quote: PCR was performed using GoTaq Flexi DNA polymerase (Promega) to amplify both junctions using primers listed in Table 1 and routinely 500 ng of genomic DNA from parental cells or each recombinant clone as template in 25 μl reactions ...
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Hepatitis B Virus genomes associate with cellular sites of DNA damage by inducing replication stressbioRxiv - Microbiology 2024Quote: ... transferred to a PCR clean-up column (Promega, A9282) and centrifuged ...
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bioRxiv - Microbiology 2023Quote: ... Each PCR reaction contained 0.25 µl Taq Polymerase (Promega), 2.5 µl 10 uM forward and reverse primers ...
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bioRxiv - Biochemistry 2024Quote: ... the PCR products were ligated (T4 DNA ligase, Promega) into similarly restricted pYES2 ...
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bioRxiv - Plant Biology 2024Quote: ... Genotyping PCRs were conducted using GoTaq DNA Polymerase (Promega) in accordance with the manufacturer’s guidelines ...
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bioRxiv - Cell Biology 2020Quote: ... Then samples were diluted 3 times in the buffer and 1 µg trypsin (Gold. Promega) was added overnight at 30°C ...
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bioRxiv - Cell Biology 2020Quote: ... followed by a 4 times dilution in Tris buffer and an overnight trypsin digestion (Promega) at a ratio 1/100 ...
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bioRxiv - Immunology 2023Quote: ... Luminescence was recorded with a 0.3 second integration time on a GloMax luminometer system (Promega).
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bioRxiv - Immunology 2023Quote: ... Luminescence was recorded with a 0.3 second integration time on a GloMax luminometer system (Promega).
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bioRxiv - Cancer Biology 2023Quote: ... In this time the Fugene HD mix (8.6 μl Fugene HD (Promega, Madison, WI, USA) in 110 μl Opti-MEM was prepared and mixed thoroughly ...
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bioRxiv - Microbiology 2022Quote: ... then dipped in a well of a 96-well PCR plate filled with 20 µl of a gyrB PCR mix (4 µl of 5x GoTaq buffer (Promega, Madison, WI, USA), 0.4 µl of 10mM dNTP ...
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bioRxiv - Molecular Biology 2020Quote: ... The expression of extracellular HiBiT-BNLN was normalized to the total intracellular pool of HiBiT-BNLN by lysing the cells and measuring luminescence with Nano-Glo HiBiT lytic detection system (Promega, N2422), following the protocol.
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bioRxiv - Neuroscience 2021Quote: ... For HiBiT detection, 25µL of HiBiT lytic reagent (1:25 substrate, 1:50 LgBiT) was added to the lysate (Promega, cat# N3050). For FFluc detection ...
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bioRxiv - Synthetic Biology 2020Quote: ... 5 μL normalised extract were diluted to 30 μL in passive lysis buffer and mixed with 30 μL Nano-Glo® HiBiT Extracellular Detection Reagent (Promega) and luminescence was detected as above ...
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bioRxiv - Synthetic Biology 2021Quote: The effect of DMSO and methyl ester version compounds (compound 34–38) on cell viability was determined using a CellTiter-GloTM ATP detection system (Promega #7573). For testing DMSO tolerance ...
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bioRxiv - Genetics 2021Quote: ... The luciferin is then utilized in a firefly luciferase reaction to generate a bright luminescent signal determined as RLUs using a GloMax-Multi Detection System: Luminometer (Promega, USA). 2/ Colorimetric detection of senescence-associated β galactosidase following the manufacturer’s protocol (Cell Signaling #9860) ...
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bioRxiv - Cell Biology 2020Quote: ... The assay plates were then equilibrated to room temperature for 15 minutes before the addition of 25 µl of Nano-Glo HiBiT Lytic Detection System (Promega #N3030.) The plates were then shaken at 300 rpm for 2 minutes on an orbital shaker ...