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Citations for Promega :
1101 - 1150 of 3110 citations for Recombinant Human High Mobility Group Box 1 His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2022Quote: ... First-strand cDNA was synthesised using 1 µg RNA and 500 ng µl-1 Oligo(dT)15 primer (Promega, Southampton, UK), denatured at 65°C for 5 min ...
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bioRxiv - Molecular Biology 2024Quote: ... 1 ml ice-cold wash buffer 1 (1x PBS with 2 % BSA, 1 mM DTT and 0.5 U/µl RNasin® Plus Ribonuclease Inhibitor - Promega #N2615) was added and cells were spun at 500 g for 3 min at 4 C ...
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bioRxiv - Physiology 2024Quote: ... further steps were performed according to the manufacturer’s protocol using proteases trypsin and Lys-C (2 h at 47° C, 1:15 and 1:30, respectively, Promega, USA). Peptides (20 μg ...
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bioRxiv - Cell Biology 2024Quote: ... The HMEC cell lines stably expressing doxycycline-inducible Rb variants were generated by transfecting cells with 1 µg of doxycycline- inducible plasmid and 1 µg of PiggyBac Transposase plasmid using the FuGene HD reagent (Promega E2311). Zeocin (300 µg/ml ...
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bioRxiv - Molecular Biology 2021Quote: ... secondary antibody staining was performed for 1 h at room temperature using anti-rabbit HRP or anti-mouse HRP secondary antibodies (Promega, 1:10,000) in blocking buffer ...
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bioRxiv - Synthetic Biology 2020Quote: ... 1:2000) and the bound primary antibodies were detected by a HRP-conjugated goat anti-rabbit secondary antibody (W401B, Promega; 1:2000). Membranes were developed and visualized using the Pierce™ ECL immunoblotting substrate (ThermoFisher Scientific).
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bioRxiv - Cell Biology 2022Quote: ... 30 mM Tris-HCl (pH 7), 1% Triton X-100, 1% NaDOC, 100 μg/mL cycloheximide (Applichem, Germany) and 30 U/mL RNase Inhibitor (Promega, United States)] ...
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bioRxiv - Biochemistry 2022Quote: ... The proteins were digested with 1 μg lysyl endopeptidase (LysC) (FUJIFILM Wako Pure Chemical Corporation) and 1 μg trypsin (Promega, Tokyo, Japan) overnight at 37°C on a shaking incubator ...
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bioRxiv - Microbiology 2021Quote: ... The CaptureSelect Biotin anti-C-tag Conjugate (LifeTechnologies #7103252100; 1/4000) was mixed with Streptavidin Alkaline Phosphatase (Promega #V5591; 1/1000 dilution) for 10 minutes before use ...
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bioRxiv - Microbiology 2021Quote: ... The reaction mixture was prepared into 96-well plates as follows: 1 μl of 1 mM amino acid mixture minus methionine (Promega, Cat: L9961), 35 μl of rabbit reticulocyte lysate ...
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bioRxiv - Cancer Biology 2020Quote: ... and diluted to 1 M urea with 200 mM ammonium bicarbonate for trypsin digestion (1 µg, 37°C, 8 hr, Promega cat # V5113). After digestion ...
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bioRxiv - Microbiology 2019Quote: ... For transfection 0.5 or 2 µg of plasmid DNA in 25 or 200 µL serum-free medium were mixed with 1 or 4 µL of FuGENE HD transfection reagent (DNA to reagent ratio of 1:2, Promega, Mannheim, Germany) and incubated for 10 min at RT ...
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bioRxiv - Immunology 2020Quote: Caspase-1 and Caspase 3/7 activities were measured using Caspase-Glo® 1 and Caspase-Glo® 3/7 assay (Promega) respectively ...
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bioRxiv - Microbiology 2020Quote: ... The protein mixture was diluted four times and digested with LysC at 1:50(w/w) for four hours and then with Trypsin at 1:40 (w/w) (Promega, http://www.promega.com/) overnight.
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bioRxiv - Biochemistry 2024Quote: ... In-solution digestion was performed with LysC-Trypsin mix (1:100 enzyme: protein ratio) and trypsin (Promega; 1:50 enzyme: protein ratio). Peptides were desalted on C18 stage tips ...
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bioRxiv - Molecular Biology 2022Quote: ... we prepared a master mix containing the membrane-permeable Nluc substrate and a membrane-impermeable Nluc inhibitor using a 1:1000 dilution of Extracellular NanoLuc Inhibitor and a 1:333 dilution of NanoBRETTM Nano-Glo Substrate into PBS (Promega, Madison, WI). Aliquots of the Nluc substrate/inhibitor master mix (100 µl ...
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bioRxiv - Biochemistry 2022Quote: ... secondary antibody staining was performed for 1 h at room temperature using antirabbit HRP or anti-mouse HRP secondary antibodies (Promega,1:10,000) in blocking buffer ...
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bioRxiv - Synthetic Biology 2023Quote: ... The proteins were digested with 1 μg of lysyl endopeptidase (LysC) (FUJIFILM Wako Pure Chemical Corporation) and 1 μg of trypsin (Promega, Tokyo, Japan) overnight at 37 °C in a shaking incubator ...
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bioRxiv - Cell Biology 2023Quote: ... and 10μg of pLKO.1-mCherry-scrambled shRNA or pLKO.1-mCherry-IRE1α shRNA using FuGENE-HD reagent (Promega, Madison, WI, USA). After 24 h of transfection ...
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bioRxiv - Microbiology 2020Quote: ... 0.3% xylose and 1% low melting temperature agarose (Promega) on slides ...
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bioRxiv - Developmental Biology 2021Quote: ... and 300 U mL-1 RNase inhibitor (Promega, N2112S). The mixture was subsequently incubated with 20 µL of Dynabeads protein-A (Life Technologies ...
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bioRxiv - Developmental Biology 2021Quote: ... and 300 U mL-1 RNase inhibitor (Promega, N2112S). After the addition of 50 µL Dynabeads protein-A (Life Technologies ...
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bioRxiv - Neuroscience 2022Quote: ... anti-b-111-tubulin (mouse, Promega G712A, 1:500), Cleaved caspase-3 (rabbit ...
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bioRxiv - Microbiology 2022Quote: ... 1 µL of RNAse inhibitor (40 U/µl, Promega) was added to stop the reaction ...
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bioRxiv - Molecular Biology 2019Quote: Goat anti-mouse IgG HRP 1:2000 (Promega W4021)
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bioRxiv - Molecular Biology 2021Quote: ... rabbit anti-NLuc (kind gift of Promega, 1:1000) and b-actin-peroxidase (Sigma ...
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bioRxiv - Molecular Biology 2021Quote: ... rabbit anti-NLuc (kind gift of Promega, 1:100). The following Alexa Fluor secondary antibodies were used ...
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bioRxiv - Developmental Biology 2022Quote: ... Anti–β-galactosidase monoclonal mouse antibody (Promega, 1:500) was co-incubated with anti-mCherry polyclonal rabbit antibody (Bio Vision ...
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bioRxiv - Biochemistry 2020Quote: ... 1x reaction buffer (from Promega (containing 1 mM Co2+) for Supplementary Fig ...
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Neuronal L-Type Calcium Channel Signaling to the Nucleus Requires a Novel CaMKIIα-Shank3 InteractionbioRxiv - Neuroscience 2019Quote: ... HRP-conjugated anti-mouse (Promega, catalog W4021, 1:6000), HRP-conjugated anti-goat (Abcam ...
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Neuronal L-Type Calcium Channel Signaling to the Nucleus Requires a Novel CaMKIIα-Shank3 InteractionbioRxiv - Neuroscience 2019Quote: ... HRP-conjugated anti-rabbit (Promega, catalog W4011, 1:6000), HRP-conjugated anti-mouse (Promega ...
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bioRxiv - Bioengineering 2020Quote: ... and 1 unit T4 ligase with associated buffer (Promega). The following thermocycler program was run ...
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bioRxiv - Biochemistry 2021Quote: ... 1 mL of streptavidin-coupled magnetic beads (Promega, Z5481) per condition were washed three times during 10 min at 4°C (under intermittent shaking ...
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bioRxiv - Biochemistry 2020Quote: ... 1 µg of sequencing-grade modified trypsin (Promega, USA) was added to each solution ...
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bioRxiv - Neuroscience 2020Quote: ... and Donkey anti-rabbit HRP (Promega W401B, 1:10,000). Soluble protein samples were normalized to REVERT total protein stain (Li-Cor).
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bioRxiv - Cell Biology 2021Quote: ... with freshly added Halt protease inhibitor (1:100, Promega)) ...
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bioRxiv - Neuroscience 2020Quote: ... US) and TubβIII (mouse, 1:1000, G712A, Promega, UK). Slides were then incubated with the appropriate fluorophore-conjugated secondary antibodies ...
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bioRxiv - Neuroscience 2019Quote: ... The HRP-conjugated goat anti-mouse (1:10,000; Promega) and goat anti-rabbit IgG (1:10,000 ...
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bioRxiv - Microbiology 2019Quote: ... 100 μL of RQ-1 RNase free DNase (Promega) was added to the column and incubated at 25°C for 15 minutes ...
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bioRxiv - Biophysics 2020Quote: ... Trypsin was added to 1:50 (m/m) (Promega) and incubated overnight at 37°C with 600 rpm shaking on a thermomixer ...
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bioRxiv - Cell Biology 2021Quote: ... 4 mM isopropyl β-d-1-thiogalactopyranoside (IPTG; Promega) was added to each RNAi well for induction of dsRNA synthesis and plates were incubated for 3-4 hours at 30°C and 155 rpm ...
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bioRxiv - Genomics 2022Quote: ... or anti-Mouse IgG HRP Conjugate (Promega, 1:2,000) diluted in blocking buffer followed by washing in TBST ...
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bioRxiv - Molecular Biology 2022Quote: ... 1 μl of 0.2 mg/ml LysC protease (Promega) in 50 mM HEPES pH 8.5 was added and proteins were pre-digested for 2 hours rotating at room temperature ...
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bioRxiv - Cell Biology 2022Quote: ... followed by treatment with 1 µg/ml trypsin (Promega). Triton X-100 treatment was carried out on ice for 30 minutes ...
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bioRxiv - Immunology 2022Quote: ... 16 % dimethylformamide) supplemented with 0.5 µl 1 % Digitonin (Promega) and 5 µl Tn5 (Illumina ...
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bioRxiv - Cell Biology 2022Quote: ... and anti-rabbit IgG HRP (Promega, WB 1:10,000)
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bioRxiv - Biophysics 2022Quote: Cells were lysed in 1× passive lysis buffer (Promega) and combined with 4× Laemmli sample buffer (Bio-Rad ...
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bioRxiv - Cell Biology 2022Quote: ... and anti-rabbit IgG HRP (Promega, WB 1:10000).
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bioRxiv - Neuroscience 2019Quote: ... in 1 × PCR thermo DNA polymerase reaction buffer (Promega). Following the addition of 100 pMol each 5’ and 3’ primer and MgCl2 to 4 mM ...
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bioRxiv - Immunology 2020Quote: ... 1 µl RNAsin Plus RNAse inhibitor (10K, Promega AG), 1 µl Superscript III (200 U/ml ...